Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway.In this study,D.officinale were grown aeroponically with light/dark cycles of 12 h/1...Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway.In this study,D.officinale were grown aeroponically with light/dark cycles of 12 h/12 h,4 h/4 h,and 2 h/2 h for 150 d.The photosynthetic electron transfer characteristics,photosynthetic CO_(2) fixation pathways,and accumulations of biomass and soluble polysaccharides in D.officinale leaves were studied.The results showed that the photosynthetic apparatus states of D.officinale in aeroponic cultivation under short light/dark cycles of 4 h/4 h and 2 h/2 h were better than that under 12 h/12 h.The dark net CO_(2) exchange percentages of D.officinale were negative in short light/dark cycles of 4 h/4 h and 2 h/2 h,and the daily net CO_(2) exchange amount and dry/fresh weight increases were doubled compared with those in 12 h/12 h light/dark cycle.High soluble polysaccharides content and the soluble polysaccharides yield of D.officinale were obtained in the shorter light/dark cycle of 2 h/2 h.Therefore,the photosynthetic pathway of D.officinale could be switched from CAM to C3 by short light/dark cycles of 4 h/4 h and 2 h/2 h,and its higher biomass accumulation and soluble polysaccharides yield could be obtained by the shorter light/dark cycle of 2 h/2 h in aeroponic cultivation.展开更多
为了制定标准车辆能耗规范和标定车辆排放,优化汽车使用性能,以呼和浩特市区道路上轻型汽车为研究对象,对轻型汽车行驶工况进行了分析.首先通过中国汽车检测工况研究和开发(China automotive test cycle,CATC)专用数据采集设备,收集了7...为了制定标准车辆能耗规范和标定车辆排放,优化汽车使用性能,以呼和浩特市区道路上轻型汽车为研究对象,对轻型汽车行驶工况进行了分析.首先通过中国汽车检测工况研究和开发(China automotive test cycle,CATC)专用数据采集设备,收集了74台车辆的行驶工况样本数据,数据采集覆盖所有时段类型、道路类型、轻型车类型和驾驶员类型;其次通过加权二次构建控制不同车辆类型的比例,采用主成分分析和聚类分析预处理数据,制定短行程规则;最后将运动学片段进行裁剪和特征值分类,构建了城市道路轻型汽车行驶工况.研究结果表明:CATC平均速度为25.87 km/h,运行平均速度为33.92 km/h、匀速比例为20.59%,怠速比例为23.72%,加速比例为28.56%,减速比例为27.13%.与欧盟提出的轻型车循环测试工况相对比,平均速度、运行平均速度、匀速比例低于欧洲工况,加速比例、减速比例、怠速比例高于欧洲工况.展开更多
基金supported by China Agriculture Research System(Grant No.CARS-21)National Natural Science Foundation of China(Grant No.31372089)。
文摘Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway.In this study,D.officinale were grown aeroponically with light/dark cycles of 12 h/12 h,4 h/4 h,and 2 h/2 h for 150 d.The photosynthetic electron transfer characteristics,photosynthetic CO_(2) fixation pathways,and accumulations of biomass and soluble polysaccharides in D.officinale leaves were studied.The results showed that the photosynthetic apparatus states of D.officinale in aeroponic cultivation under short light/dark cycles of 4 h/4 h and 2 h/2 h were better than that under 12 h/12 h.The dark net CO_(2) exchange percentages of D.officinale were negative in short light/dark cycles of 4 h/4 h and 2 h/2 h,and the daily net CO_(2) exchange amount and dry/fresh weight increases were doubled compared with those in 12 h/12 h light/dark cycle.High soluble polysaccharides content and the soluble polysaccharides yield of D.officinale were obtained in the shorter light/dark cycle of 2 h/2 h.Therefore,the photosynthetic pathway of D.officinale could be switched from CAM to C3 by short light/dark cycles of 4 h/4 h and 2 h/2 h,and its higher biomass accumulation and soluble polysaccharides yield could be obtained by the shorter light/dark cycle of 2 h/2 h in aeroponic cultivation.
