Investigation of ultraweak emissions in the processes of chemiluminescence, CL, and electrochemiluminesce, ECL, requires special techniques of their recording and spectral analysis. From among the hitherto proposed me...Investigation of ultraweak emissions in the processes of chemiluminescence, CL, and electrochemiluminesce, ECL, requires special techniques of their recording and spectral analysis. From among the hitherto proposed methods of detection of the emission spectra of these processes, that of the cut-off filter was most sensitive. The usefulness of this method in interpretation of the CL and ECL systems of the quantum yields in the range 1×10^-9-1×10^-11 containing ions and complexes of Eu（Ⅲ）, Tb（Ⅲ） and Dy0ID was shown. Exceptional character of the emission bands of lanthanide ions, being a result of the f-f electron transitions and in particular their low FWHM, permitted the appli- cation of the cut-off filter method to their analysis. The results obtained for CL and ECL on the basis of analysis of ultraweak emission proved to be successful in analytical applications. The systems containing Eu（Ⅲ） ions hydrated or complexed with organic ligands enabled inferring changes in the coordination sphere of the ions.展开更多
Objective:To compare the effects of clinical application of chemiluminescence and real-time,fluorescence-based quantitative PCR in the detection Epstein-Barr virus(EBV).Methods:The data of chemiluminescence and real-t...Objective:To compare the effects of clinical application of chemiluminescence and real-time,fluorescence-based quantitative PCR in the detection Epstein-Barr virus(EBV).Methods:The data of chemiluminescence and real-time fluorescent quantitative PCR.fromipaEsfwo were suspected of being infectea w1tn rito1 roro January 2016 to January 2019 in our hospital were analyzed.The specific stage of EBV infection was analyzed,and the differences in results of the two detection methods were compared.Results:Chemiluminescence method was used to detect EBV infection during the active phase.The sensitivity of the chemiluminescence method was 76.7%(56/73)and the real-time quantitative PCRmethod was 90.4%(66/73).There was a statistical difference between the two detection methods(P<0.05).Conclusion:There was no statistical difference in positive predictive values between the chemiluminescence method and the real-time,fluorescence-based quantitative PCR method in the detection of EBV infection,but the sensitivity of chemiluminescence method is slightly lower than the real-time quantitative PCRmethod.It is noteworthy that chemiluminescence method is convenient and fast while the real-time,fluorescence-based quantitative PCR method is more accurate,which can provide a more accurate reference for clinical treatment.展开更多
Catalytic chemiluminescence of over twenty metallic ions in the systemof 2, 6, 7-tihydroxyl-9- (4'-chlorophenyl)-3-fluorone (Cl-PF )-H2O2 has been tested.Trace amount of Co(Ⅱ) strong1y catalysed this chemilumines...Catalytic chemiluminescence of over twenty metallic ions in the systemof 2, 6, 7-tihydroxyl-9- (4'-chlorophenyl)-3-fluorone (Cl-PF )-H2O2 has been tested.Trace amount of Co(Ⅱ) strong1y catalysed this chemiluminescence reaction, especiallyin presence of cationic surfactant cetyltrimethylammonium bromide (CTMAB), andthe chemiluminescence intensity was proportional to the concentraction of Co(Ⅱ) in therange 0. 5~6ng. ml-1 Co(Ⅱ). Two steps could be involved in the chemiluminescentreaction of C1-PF. In alkaline solution, the O2 radical ion should attack an oxygenbridge between the benzene rings of Cl-PF with break in oxygen bridge bond to produce reaction product which is excited by the energy derived from this chemical reaction. The electronic excited state emits the radiation(560 nm) on falling to the groundstate, followed by formation of polyphenol derivatives. the polyphenol derivatives arefurther oxidized to produce chemiluminescence (480 nm) which is due to emission fromsinglet oxygen.展开更多
Objective: To analyze the dynamic evaluation of chemiluminescence, colloidal gold, and immunofluorescence chromatography in detecting antibodies in COVID-19 patients within four weeks of infection, and to provide evid...Objective: To analyze the dynamic evaluation of chemiluminescence, colloidal gold, and immunofluorescence chromatography in detecting antibodies in COVID-19 patients within four weeks of infection, and to provide evidence for clinical application. Method: 74 patients with confirmed SARS-COV-2 infection in the local area were selected as the experimental group, while 231 patients with negative SARS-COV-2 results but not vaccinated with Covid19 vaccine were selected as the control group;during the first, second, third, and fourth weeks after enrollment in the experimental group, three methods were used to detect SARS-COV-2 IgG and IgM in patients’ blood: chemiluminescence method, colloidal gold antibody method, and immuno-fluorescence chromatography. In the control group, three methods were used to detect SARS-COV-2 IgG and IgM during physical examination for SARS-COV-2 nucleic acids. The ROC curve was drawn to analyze the value of each indicator in predicting SARS-COV-2 infection, and the kappa method was used to analyze the consistency of the detection results of each indicator. Results: There was no significant difference in the positive rates of SARS-COV-2 IgM and IgG antibodies detected by chemiluminescence, colloidal gold, and immunofluorescence chromatography during the four-week period (P > 0.05). The positive rates of SARS-COV-2 IgM and IgG antibodies detected by the three methods during the first week of infection were not higher than 60%;when the three methods were used to detect SARS-COV-2 IgM and IgG in vivo, the AUC diagnosed by the test results was less than 0.80 at the first week, the diagnostic efficacy of the three methods was above 0.95 from the second week to the fourth week, and the diagnostic efficacy of the three methods was higher than 0.97 at the fourth week. The diagnostic efficacy of the three methods was comparable;the three methods for detecting SARS-COV-2 IgM and IgG antibodies showed high consistency in four cycles. Conclusion: Chemiluminescence, colloidal gold, and immunofluorescence chromatography are highly consistent in the detection of SARS-COV-2 IgM and IgG antibodies, and can be used as an auxiliary diagnosis and efficacy observation of novel coronavirus infections according to the needs, but the positive rate of infected people in the first week is low.展开更多
基金support from the Polish Ministry of Science and Higher EducationGrant N N204 028236
文摘Investigation of ultraweak emissions in the processes of chemiluminescence, CL, and electrochemiluminesce, ECL, requires special techniques of their recording and spectral analysis. From among the hitherto proposed methods of detection of the emission spectra of these processes, that of the cut-off filter was most sensitive. The usefulness of this method in interpretation of the CL and ECL systems of the quantum yields in the range 1×10^-9-1×10^-11 containing ions and complexes of Eu（Ⅲ）, Tb（Ⅲ） and Dy0ID was shown. Exceptional character of the emission bands of lanthanide ions, being a result of the f-f electron transitions and in particular their low FWHM, permitted the appli- cation of the cut-off filter method to their analysis. The results obtained for CL and ECL on the basis of analysis of ultraweak emission proved to be successful in analytical applications. The systems containing Eu（Ⅲ） ions hydrated or complexed with organic ligands enabled inferring changes in the coordination sphere of the ions.
文摘Objective:To compare the effects of clinical application of chemiluminescence and real-time,fluorescence-based quantitative PCR in the detection Epstein-Barr virus(EBV).Methods:The data of chemiluminescence and real-time fluorescent quantitative PCR.fromipaEsfwo were suspected of being infectea w1tn rito1 roro January 2016 to January 2019 in our hospital were analyzed.The specific stage of EBV infection was analyzed,and the differences in results of the two detection methods were compared.Results:Chemiluminescence method was used to detect EBV infection during the active phase.The sensitivity of the chemiluminescence method was 76.7%(56/73)and the real-time quantitative PCRmethod was 90.4%(66/73).There was a statistical difference between the two detection methods(P<0.05).Conclusion:There was no statistical difference in positive predictive values between the chemiluminescence method and the real-time,fluorescence-based quantitative PCR method in the detection of EBV infection,but the sensitivity of chemiluminescence method is slightly lower than the real-time quantitative PCRmethod.It is noteworthy that chemiluminescence method is convenient and fast while the real-time,fluorescence-based quantitative PCR method is more accurate,which can provide a more accurate reference for clinical treatment.
文摘Catalytic chemiluminescence of over twenty metallic ions in the systemof 2, 6, 7-tihydroxyl-9- (4'-chlorophenyl)-3-fluorone (Cl-PF )-H2O2 has been tested.Trace amount of Co(Ⅱ) strong1y catalysed this chemiluminescence reaction, especiallyin presence of cationic surfactant cetyltrimethylammonium bromide (CTMAB), andthe chemiluminescence intensity was proportional to the concentraction of Co(Ⅱ) in therange 0. 5~6ng. ml-1 Co(Ⅱ). Two steps could be involved in the chemiluminescentreaction of C1-PF. In alkaline solution, the O2 radical ion should attack an oxygenbridge between the benzene rings of Cl-PF with break in oxygen bridge bond to produce reaction product which is excited by the energy derived from this chemical reaction. The electronic excited state emits the radiation(560 nm) on falling to the groundstate, followed by formation of polyphenol derivatives. the polyphenol derivatives arefurther oxidized to produce chemiluminescence (480 nm) which is due to emission fromsinglet oxygen.
文摘Objective: To analyze the dynamic evaluation of chemiluminescence, colloidal gold, and immunofluorescence chromatography in detecting antibodies in COVID-19 patients within four weeks of infection, and to provide evidence for clinical application. Method: 74 patients with confirmed SARS-COV-2 infection in the local area were selected as the experimental group, while 231 patients with negative SARS-COV-2 results but not vaccinated with Covid19 vaccine were selected as the control group;during the first, second, third, and fourth weeks after enrollment in the experimental group, three methods were used to detect SARS-COV-2 IgG and IgM in patients’ blood: chemiluminescence method, colloidal gold antibody method, and immuno-fluorescence chromatography. In the control group, three methods were used to detect SARS-COV-2 IgG and IgM during physical examination for SARS-COV-2 nucleic acids. The ROC curve was drawn to analyze the value of each indicator in predicting SARS-COV-2 infection, and the kappa method was used to analyze the consistency of the detection results of each indicator. Results: There was no significant difference in the positive rates of SARS-COV-2 IgM and IgG antibodies detected by chemiluminescence, colloidal gold, and immunofluorescence chromatography during the four-week period (P > 0.05). The positive rates of SARS-COV-2 IgM and IgG antibodies detected by the three methods during the first week of infection were not higher than 60%;when the three methods were used to detect SARS-COV-2 IgM and IgG in vivo, the AUC diagnosed by the test results was less than 0.80 at the first week, the diagnostic efficacy of the three methods was above 0.95 from the second week to the fourth week, and the diagnostic efficacy of the three methods was higher than 0.97 at the fourth week. The diagnostic efficacy of the three methods was comparable;the three methods for detecting SARS-COV-2 IgM and IgG antibodies showed high consistency in four cycles. Conclusion: Chemiluminescence, colloidal gold, and immunofluorescence chromatography are highly consistent in the detection of SARS-COV-2 IgM and IgG antibodies, and can be used as an auxiliary diagnosis and efficacy observation of novel coronavirus infections according to the needs, but the positive rate of infected people in the first week is low.
