Positive regulatory role of R2R3 MYBs in terpene biosynthesis in Lilium‘Siberia’

Terpenoids are the main components contributing to the fragrance of Lilium‘Siberia’,and LiTPS2 plays a critical role in the biosynthesis of monoterpenoids.Although the major terpene synthases in Lilium‘Siberia’have been identified,how these TPS genes are transcriptionally regulated remains elusive in this distinguished flower.This study aimed to identify transcription factors that regulate the terpene synthesis in Lilium,and disclose the related underlying transcriptional regulation mechanism.In this study,we identified three R2R3-MYB TFs—LiMYB1,LiMYB305 and LiMYB330,which were involved in regulating the biosynthesis of terpenes in Lilium‘Siberia’.Quantitative real-time PCR showed spatial and temporal expression patterns consistent with the emission patterns of terpene compounds.When LiMYB1,LiMYB305 and LiMYB330were overexpressed in flowers,the release of some main monoterpenes,such as linalool and ocimene,as well as the expression of TPS genes,especially for LiTPS2,were enhanced.A virus-induced gene silencing(VIGS)assay showed that silencing these three LiMYBs decreased the level of monoterpenes by down-regulating the expression of the TPS genes.The yeast one-hybrid and transient expression assays indicated that all three LiMYBs could bind to and activate the promoter of LiTPS2.Moreover,the yeast two-hybrid assay verified that LiMYB1 could interact with LiMYB308 and LiMYB330,indicating their synergistic roles in the regulation of floral terpene biosynthesis.In general,these results indicated that LiMYB1,LiMYB305,and LiMYB330 might play essential roles in terpene biosynthesis in Lilium and would provide a new perspective for the transcriptional regulation of volatile terpenes in flowers.

WRKY11 up-regulated dirigent expression to enhance lignin/lignans accumulation in Lilium regale Wilson during response to Fusarium wilt

Lilium are highly economically valuable ornamental plants that are susceptible to Fusarium wilt caused by Fusarium oxysporum.Lilium regale Wilson,a wild lily native to China,is highly resistant to F.oxysporum.In this study,a WRKY transcription factor,WRKY11,was isolated from L.regale,and its function during the interaction between L.regale and F.oxysporum was characterized.The ectopic expression of LrWRKY11 in tobacco increased the resistance to F oxysporum,moreover,the transcriptome sequencing and UHPLC-MS/MS analysis indicated that the methyl salicylate and methyl jasmonate levels rose in LrWRKY11 transgenic tobacco,meanwhile,the expression of lignin/lignans biosynthesis-related genes including a dirigent(DiR)was up-regulated.The lignin/lignans contents in LrWRKY11-transgenic tobacco also significantly increased compared with the wild-type tobacco.In addition,the resistance of L.regale scales in which LrWRKY11 expression was silenced by RNAi evidently decreased,and additionally,the expression of lignin/lignans biosynthesis-related genes including LrDIR1 was significantly suppressed.Therefore,LrDIR1 and its promoter(PLrDIR1)sequence containing the W-box element were isolated from L.regale.The interaction assay indicated that LrWRKY11 specifically bound to the W-box element in PLrDIR1 and activated LrDIR1 expression.Additionally,β-glucuronidase activity in the transgenic tobacco co-expressing LrWRKY11/PLrDIR1-β-glucuronidase was higher than that in transgenic tobacco expressing PLrDIR1-β-glucuronidase alone.Furthermore,the ectopic expression of LrDIR1 in tobacco enhanced the resistance to F.oxysporum and increased the lignin/lignans accumulation.In brief,this study revealed that LrWRKY11 positively regulated L.regale resistance to F.oxysporum through interaction with salicylic acid/jasmonic acid signaling pathways and modulating LrDIR1 expression to accumulate lignin/lignans.

R2R3-type Lo MYB21 affects jasmonate-regulated development and dehiscence of anthers in lily(Lilium oriental hybrids)

Lilies are widely cultivated for cut flowers,but their large anthers carry a considerable amount of colored pollen that is dispersed easily.Studying the molecular mechanism of anther development and dehiscence could help solve this problem.LoMYB21,encoding a putative R2R3v-myb avian myeloblastosis viral oncogene homolog(MYB)transcription factor,was identified from oriental lilies(Lilium‘Siberia’).Real-time quantitative PCR analysis showed that LoMYB21 was mainly expressed in the anther,filament and stigma and had high expression during the late stages of lily anther development.LoMYB21 had transactivation activity and was located in the nucleus through yeast one-hybrid assays and transient expression in Nicotiana benthamiana.Suppression of LoMYB21 by virus-induced gene silencing(VIGS)in Lilium‘Siberia’led to anther indehiscence and reduced the expression of genes related to Jasmonate acid(JA)biosynthesis and signal transduction.Induction of LoMYB21 in DEX::LoMYB21 transgenic Arabidopsis caused procumbent inflorescences that became infertile,accompanied by higher expression of JA biosynthetic and signaling genes.These results demonstrated that JA content and signaling were abnormal in silenced lily and transgenic LoMYB21 Arabidopsis,which affected anther development.Our study indicated that LoMYB21 could regulate lily anther dehiscence through JA biosynthesis and signaling during the late stages of anther development.

Monitoring Xylem Transport in the Stem of Lilium lancifolium Using Fluorescent Dye 5(6)-Carboxyfluorescein Diacetate

The xylem undergoes physiological changes in response to various environmental conditions during the process of plant growth.To understand these physiological changes,it is extremely important to observe the transport of xylem.In this study,the distribution and structure of vascular bundle in Lilium lancifolium were observed using the method of semithin section.Methods for introducing a fluorescent tracer into the xylem of the stems were evaluated.Then,the transport rule of 5(6)-Carboxyfluorescein diacetate(CFDA)in the xylem of the stem of L.lancifolium was studied by fluorescence dye in live cells tracer technology.The results showed that the vascular bundles of L.lancifolium were scattered in the basic tissue,the peripheral vascular bundles were smaller and densely distributed,and the closer to the center,the larger the volume of vascular bundles and the more sparsely distributed.The vascular bundles of L.lancifolium are limited external tenacity vascular bundles,which are composed of phloem and xylem.The most suitable method for CFDA labeling the xylem of isolated stem segments of L.lancifolium was solution soaking for 24 h.The running speed of CF in the isolated stem was 0.3 cm/h,which was consistent with the running speed of the material in the field.CF could be transported between the xylem and parenchyma cells,indicating that the material transport in the xylem could be through the symplastic pathway.The above results laid a foundation for the study of the xylem transport mechanism and the xylem pathogen disease of lily.

Interdecadal changes in the western Siberian summer mean and extreme rainfall during 1982-2021

本文研究发现,西西伯利亚夏季降水在1995年后显著增加,2012年后又有所减少,极端降水日数和强度呈现一致的变化特征.伴随这两次降水的年代际增加/减少,西西伯利亚上空出现异常气旋/反气旋和水汽幅合/幅散.极端降水发生时的区域环流特征在三个时段基本一致:西西伯利亚低空出现气旋性异常,高空为西北-东南向的异常气旋-反气旋偶极子型.1995-2011年期间,极端降水日数更多,强度更强,上述异常环流型也更强.

Human responses to flower fragrance of Lilium ‘Siberia’ and Rosa ‘Escimo’

The objective of our study was to lay a foundation for the effect of flower flagrance on human emotions and to provide a theory for the choice of indoor plants and the improvement of the olfactory environment. Specifically, our purpose was to study human physiological responses to flower fragrance of Lilium ＇Siberia＇ and Rosa ＇Escimo＇. The participants were 31 college students. Blood pressure, pulse rate, finger temperature （FT） and galvanic skin response （GSR） were measured. The results show that the fragrance of Rosa ＇Escimo＇ causes the diastolic pressure and pulse rate of the participants to reduce significantly. The average decrease was 0.37 kPa and 2.23 beats per minute, which indicates that sympathetic nervous activity increases, physiological arousal decreases and emotional alleviation occurs. Furthermore, the GSR of participants significantly increased by smelling the fragrance of Lilium ＇Siberia＇, indicating that both sympathetic nervous activity and physiological arousal increased. But the data could not prove that flower fragrance stimulation has an effect on changes in systolic pressure and finger temperature. Some other factors, such as basic emotion and weather, may have an effect.

东方百合‘Siberia’正反交及胚挽救

以‘Siberia’‘Martinique’‘After Eight O′clock’‘Sunshine Iceland’和‘Bonaire’为亲本进行杂交育种,并进行正反交实验,比较正反交结实率及有胚率;采用胚挽救技术克服受精后障碍,比较不同杂交组合、胚龄、培养方式、培养基对幼胚生长萌发的影响,以期建立一套适合东方百合‘Siberia’的正反交及胚挽救方案,创制东方百合新种质。结果表明:‘Siberia’与‘Martinique’‘After Eight O′clock’正反交存在受精障碍,但均获得了膨大子房及有胚种子;其中,‘Siberia’作父本的有胚率显著高于其作母本,在‘Siberia’与‘After Eight O′clock’组合中,其作父本的有胚率是其作母本的4.5倍;在‘Siberia’בMartinique’组合中有胚率是其作母本的2.3倍;在‘Siberia’בBonaire’杂交组合中有胚率是其作母本的1.01倍;在‘Siberia’בSunshine Iceland’杂交组合中有胚率是其作母本的1.2倍。胚挽救试验结果显示,70 d胚龄的幼胚最适合剥除胚乳培养,在‘Siberia’בSunshine Iceland’杂交组合中,其剥除胚乳培养的萌发率最高,是未剥除胚乳培养的5.5倍;胚离体培养的最佳培养基为MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA+0.3%蔗糖。杂交后的种子在播种20 d左右开始萌动,萌发率在0.8%~8.7%,40 d左右出苗率显著提高,其中在‘Siberia’בBonaire’杂交组合中最高达64.3%。

兰州百合鳞茎花青素合成相关MYB与bHLH转录因子的筛选分析

百合鳞茎在采后贮藏过程中,由于花青素的积累导致鳞茎变紫红色,进而影响其价值。该文基于兰州百合鳞茎转录组数据,利用生物信息学技术分析花青素相关转录因子,共分析了50个MYB转录因子与73个bHLH转录因子,包括序列的比对、理化性质分析、亚细胞定位、系统进化关系以及保守结构域预测。MYB分类结果显示,50个MYB转录因子中有2个属于1R-MYB类,44个属于R2R3-MYB类,3个属于3R-MYB类,1个属于4R-MYB类。50个MYB不稳定蛋白均为亲水性蛋白,亚细胞定位表明48个MYB定位于细胞核。73个bHLH均为不稳定蛋白,且72个都为亲水性,亚细胞定位显示58个bHLH定位于细胞核。通过与模式植物拟南芥MYB和bHLH转录因子构建进化树分析、保守结构域预测、基因表达量分析以及转录因子与结构基因的相关性分析,初步筛选出3个MYB基因和1个bHLH基因可促进兰州百合鳞茎花青素合成,为进一步深入研究兰州百合鳞茎花青素调控基因提供了理论支持。

Spatial analysis of vegetation cover response to climate trends in Khakassia(South Siberia)

An analysis of the changes in vegetation cover on the territory of the Republic of Khakassia in 2000–2021 due to climatic trends was carried out based on the MODIS data.The changes in vegetation cover were estimated based on trends in Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index(EVI).In general,in the 21st century,an increase in the biomass of vegetation cover is observed.Positive trends were observed in 16%–22%of the territory,and negative only in 1%–3%.For about 20%of the analyzed territory,a significant influence of climate on the changes in vegetation cover was revealed.The most pronounced negative impact on vegetation cover was caused by summer air and soil temperatures,spring temperature,and summer winds,and the positive impact was caused by summer precipitation and soil moisture.The response of the vegetation cover to climate was non-uniform concerning the topography.Thus,a significant correlation with the amount of precipitation was observed for~20%–35%of vegetation growing below 600 m above sea level and for less than 5%above this elevation.The negative effect of summer temperatures on plants prevailed mainly at an elevation below~1400 m above sea level.Projected climate change is likely to lead to significant degradation of vegetation in the steppe and foreststeppe in Khakassia in the coming decades.

Synoptic-scale reversal of dipole surface temperature anomalies over East Asia and Central Siberia in November 2021

2021年11月,东亚与中西伯利亚经历了相反的冷暖异常转换,表现为“中西伯利亚偏冷,东亚偏暖”与“中西伯利亚偏暖,东亚偏冷”的交替出现.该偶极型气温异常的天气尺度反转伴随着大尺度大气环流异常的反转.进一步分析表明,东亚与中西伯利亚的偶极型气温异常反转是1979-2021年期间11月欧亚气温日变化的主导模态之一(发生概率超过56%).

基于转录组信息的百合SWEET基因家族鉴定及表达特性分析

以东方百合品种‘西伯利亚’为试材,采用生物信息学方法,研究了百合SWEET基因家族成员编码蛋白的理化性质、二级结构、系统进化、保守结构域、Motif及基因表达模式,对百合SWEET基因家族成员进行鉴定,探究其在鳞茎发育过程中的作用,以期为该类基因生物学功能和百合地下茎生鳞茎发育机制的研究提供参考依据。结果表明:从转录组数据鉴定到了12个SWEET基因,所有家族成员都为稳定的疏水蛋白,且均含有MtN3_slv结构域,可划分为4个亚家族,不同成员在百合茎生鳞茎形成和进一步发育中差异表达。

百合育种技术研究进展

百合(Lilium spp.)是多年生球根草本植物,包括观赏、食用和药用百合,具有较高的经济价值。百合遗传背景复杂、杂合度高、远缘杂交不亲和,且传统杂交育种周期长、育种精度低,难以快速高效地培育目标品种。目前,包括现代杂交育种、诱变育种、倍性育种、体细胞杂交育种和基因工程育种在内的现代育种技术均已在百合中应用。本文总结了百合杂交育种中远缘杂交不亲和的克服方法,诱变育种中的诱变条件,倍性育种中多倍体育种和单倍体育种主要采取的技术手段,体细胞杂交育种原生质体分离纯化和杂交的条件以及基因工程育种中百合遗传转化体系和基因编辑体系的研究进展。在此基础上,列举了不同百合育种方法和技术的研究案例,分析了不同育种技术面临的问题,并展望了百合育种技术的进一步发展和应用前景,旨在为未来百合育种技术及其应用的研究提供有价值的参考,并为更多百合新品种的创制提供依据。

株行距和种植深度对西伯利亚百合生长的影响

阐明不同株行距及种植深度对西伯利亚百合在栽植阶段生长的影响,总结试验栽植地最适西伯利亚百合生长的最佳株行距及种植深度,从中挖掘提高西伯利亚百合切花品质的关键种植因素,寻求绿色、高效的增产提质方式。以西伯利亚2、3代种球为试验对象,按照标准化生产流程,采用二因素随机区组设计,探讨不同株行距及种植深度对百合鲜切花的花茎高度、花苞大小及花苞数量的影响。除株行距对2代种球的花苞数量影响达5%显著水平外,株行距及种植深度对西伯利亚百合2代和3代种球的其他生长指标影响均达1%极显著差异水平。处理3即15 cm×20 cm的株行距25 cm的种植深度组合于2代和3代种球均为最佳处理方式。处理7即25 cm×30 cm的株行距15 cm的种植深度组合对2代和3代种球的花茎高度和花苞直径的生长最不利,处理4不利于种球花苞数量的生长。种植深度对于2代和3代种球花苞数量增长的影响大于株行距。而花茎高度和花苞直径则有所区别,种植深度对2代种球花茎高度生长的影响大于株行距,3代种球反之;株行距对2代种球花苞直径的影响大于种植深度,3代种球反之。于2、3代种球的花茎高度和花苞直径而言,A1B3即以15 cm×20 cm的株行距25 cm的种植深度组合能够获得良好的生长效果。而A3B3即25 cm×30 cm的株行距25 cm的种植深度的组合对2代种球的花苞数量增加有利,A1B3则利于3代种球花苞数量的增加。合适的种植深度能够使植物的根系得到良好的发育,同时能够有效地吸收土壤中的养分和水分,促进植物生长。种球种植株行距与百合花苞数量、花茎高度及花苞大小都呈显著正相关。

龙牙百合褐斑病病原的鉴定、生物学特性及室内药剂筛选

为明确龙牙百合褐斑病病原,采集龙牙百合褐斑病典型症状的叶片,进行病原物分离、纯化和致病性测定,结合病原菌形态学观察及其rDNA-ITS、gapdh、tef1、RPB2和Alt a1等基因的序列分析,确定引起龙牙百合褐斑病的病原菌为枝状链格孢Alternaria arborescens。采用菌丝生长速率法,测定了光照、温度、pH值、碳源、氮源和不同培养基等处理对该病原菌生长的影响,并测定了病原菌对7种杀菌剂的敏感性。结果表明,最适合菌丝生长的条件为温度25℃,pH值5.0,碳源为蔗糖,氮源为牛肉浸膏,培养基为马铃薯葡萄糖琼脂(PDA)培养基;菌落致死温度为50℃。在7种参试药剂中,木霉菌抑制作用最强,其半数效应浓度(EC_(50))和90%效应浓度(EC_(90))值最低,分别是8.758×10^(-5)和0.198 mg·L^(-1);噁唑菌酮代森锰锌的毒力最低,EC_(50)和EC_(90)值分别为13.713和816.937 mg·L^(-1)。本研究结果为龙牙百合褐斑病菌的精准防控提供了理论依据。

不同温度对鲜切百合鳞茎片紫红色变化的影响

在百合鳞茎的贮藏和加工过程中,鳞茎片经常发生紫红色变化的现象。为研究温度对鲜切百合鳞茎片紫红色变化的影响,采用不同温度(4、20、35℃)贮藏兰州百合鳞茎片,测定了百合鳞茎片表面色泽、总酚含量、总花青素含量、花青素合成相关酶及多酚氧化酶(polyphenol oxidase,PPO)和过氧化物酶(peroxidase,POD)活力变化。结果表明,较高的温度提高了百合鳞茎片总酚含量。在贮藏中期,温度通过促进PPO和POD的活力,增加了百合鳞茎片的褐变度,导致其表面品质下降。同时,较高的温度促进了苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL)、查尔酮合成酶(chalcone synthase,CHS)、二氢黄酮醇还原酶(dihydroflavonol reductase,DFR)和花青素合成酶(anthocyanin synthase,ANS)等花青素合成相关酶的活力,且20℃的贮藏温度可以显著增加百合鳞茎片中花青素的积累,但都不足以使百合鳞茎片表面呈现紫红色。因此,温度不是影响鲜切百合鳞茎片紫红色变化的主要因素。

在西伯利亚远东“发现”中共党史新文献和新问题

俄罗斯西伯利亚远东地区在20世纪20—50年代是联共(布)和共产国际发动东方革命和中国革命的基地。这一地区也是中国共产党在艰难困苦条件下进行组织建设、干部培养、经费筹集、物资运输的关键通道,亦是中国共产党与联共(布)、共产国际、东方国家或民族的共产党和共产主义力量保持党际和国际联系的前沿地带。在上述地区保留了大量涉及中共党史的档案文献,未被充分发掘和利用,并且有相关的中共党史新问题因档案文献的缺乏而湮灭无现。新西伯利亚州国家档案馆、哈巴罗夫斯克边疆区国家档案馆、滨海边疆区国家档案馆(海参崴)的馆藏最值得关注。

东方百合Siberia多倍体诱导及其细胞学鉴定

本文以东方百合Siberia的试管苗为试验材料,研究了预培养时间、秋水仙素处理方法及浓度等因素对诱导试管苗多倍体的影响。研究结果表明:秋水仙素溶液直接浸泡较共培养法好,且以0.05%的浓度和浸泡48h为最佳组合,最终获得了18株四倍体试管苗。通过根尖染色体计数确定其为稳定的同源四倍体。显微观测气孔特征后发现:二倍体与四倍体植株的叶下表皮气孔密度及气孔保卫细胞长、宽差异均达到了极显著水平。

卷丹百合黄酮提取物抗氧化活性研究

该实验旨在研究卷丹百合黄酮提取物抗氧化活性。以化学法、秀丽隐杆线虫模型为评价方法,用自由基清除率、抗氧化酶活力及线虫寿命等为指标,探究卷丹百合黄酮化合物的抗氧化能力。结果表明,卷丹百合黄酮提取物在料液比为1:10(g:mL)时,抗氧化活性达到最大值,其中DPPH自由基清除率为83.46%,ABTS阳离子自由基清除率为73.26%。卷丹百合黄酮提取物能显著提高线虫体内抗氧化酶活力和谷胱甘肽含量,提高线虫抗氧化能力,起到延长线虫寿命的作用。经过卷丹百合黄酮提取物处理的线虫在氧化应激条件下平均存活时间能达到10 h。卷丹百合黄酮提取物具有良好的抗氧化活性,具备开发食品抗氧化剂或功能食品的潜力。

百合鳞茎营养素与植物化学成分研究进展

百合是多年生草本植物,兼具观赏、食用和药用价值,其可食用或药用部位一般为鳞茎。近年来,百合鳞茎作为一种特色蔬菜,受到消费者青睐。本文重点从不同品种视角对百合鳞茎进行了分析,首先归纳了碳水化合物(包括活性多糖)、蛋白质及氨基酸、脂类、矿物质和维生素等营养素的详细构成,进一步总结了其甾体皂苷(包括螺甾烷醇、异螺甾烷醇、变形螺甾烷醇、呋甾烷醇类)、酚酸类(包括单聚酚酸、酚酸甘油酯和苷类)、黄酮类(包括黄酮醇、黄烷醇、查尔酮、黄烷酮、黄烷酮醇、花色苷类等)和生物碱类(包括异喹啉、吡咯啉、哌啶和甾体糖苷类)等已知植物化学成分的构成及其生物活性,最后对百合在保健食品中的应用进行概括,以期为百合研究及非药用加工利用提供理论依据和研究思路。

基于UHPLC-QTOF-MS的兰州百合植物化学成分鉴定

为探究地理标志产品兰州百合(Lilium davidii var.unicolor)的植物化学成分,以甘肃省五个主要产区(兰州市榆中县、兰州市七里河区、定西市临洮县、定西市渭源县、临夏市永靖县)的兰州百合鳞茎为研究对象,基于超高效液相色谱串联四极杆飞行时间质谱法的非靶向代谢组学技术鉴定其植物化学成分。结果表明,在兰州百合鳞茎中初步鉴定出62种植物化学成分,包含谷胱甘肽等22种氨基酸、多肽及其衍生物,溶血磷脂酰胆碱等9种油脂和磷脂类,鼠李糖等7种糖类,阿魏酸、咖啡酸等7种酚酸类,山奈酚等4种黄酮类,核黄素、维生素C等4种天然维生素,阿魏酰腐胺等3种酚胺类,大丁苷等2种香豆素类,葫芦巴碱等2种生物碱,萜类组分松香酸和三萜皂苷类组分刺五加苷E。本研究丰富了兰州百合植物化学成分的现有数据,为兰州百合的研究与利用提供了科学依据。