[ Objeelive] This study aimed to develop a rapid propagation method in a novel temporary immersion bioreactor system （TIS） for herbal plantlets com- pared with solid culture method. [ Method ] Three herbal species, ...[ Objeelive] This study aimed to develop a rapid propagation method in a novel temporary immersion bioreactor system （TIS） for herbal plantlets com- pared with solid culture method. [ Method ] Three herbal species, including Dendrobium candidum Wall. Ex IJndl （D. candidum）, Anoectochilus roxburghii （Wall.）Lindl. （A. roxburghii） and Lilium davidii var. unicolor （L. davidii）, were used and tested by TIS against solid culture method. [Result] When the two culture methods were compared, the multiplication rate of D. candidum in TIS was found to be 1 ： 24.71, which was 6.55 times to those of solid culture. The multiplication rate of A. roxburghii was higher than those of the solid culture, but the plantlets was poorer than those of solid culture at the last phase in bioreactor culture, under the culture condition tested. The multiplication rate in TIS ofL. davidii was 1：17.23 whilst the rate was only 1：4.45 on solid culture, resulting larger bulbs than those in the solid culture. [ Conclusion] The TIS designed in our study could provide a potential mean for industrial production of plandets. However, the parameters vary greatly among different species, and it is to be optimized according to plant species.展开更多
以芹菜、百合为主要原料,辅以柠檬酸、白砂糖,研制出口感优良、营养价值高的复合饮料。在单因素试验的基础上,通过正交试验设计,确定芹菜百合复合饮料的最佳配方为:百合汁和芹菜汁混合比例为1∶4(m L/m L),白砂糖6 g/100 m L,柠檬酸0.08...以芹菜、百合为主要原料,辅以柠檬酸、白砂糖,研制出口感优良、营养价值高的复合饮料。在单因素试验的基础上,通过正交试验设计,确定芹菜百合复合饮料的最佳配方为:百合汁和芹菜汁混合比例为1∶4(m L/m L),白砂糖6 g/100 m L,柠檬酸0.08 g/100 m L。通过试验,确定黄原胶0.03 g/100 m L与CMC-Na0.03 g/100 m L混合稳定剂为饮料的最佳稳定剂。此复合饮料营养丰富、风味独特,具有一定的应用前景。展开更多
基金Supported by National Natural Science Foundation of China(30971898)
文摘[ Objeelive] This study aimed to develop a rapid propagation method in a novel temporary immersion bioreactor system （TIS） for herbal plantlets com- pared with solid culture method. [ Method ] Three herbal species, including Dendrobium candidum Wall. Ex IJndl （D. candidum）, Anoectochilus roxburghii （Wall.）Lindl. （A. roxburghii） and Lilium davidii var. unicolor （L. davidii）, were used and tested by TIS against solid culture method. [Result] When the two culture methods were compared, the multiplication rate of D. candidum in TIS was found to be 1 ： 24.71, which was 6.55 times to those of solid culture. The multiplication rate of A. roxburghii was higher than those of the solid culture, but the plantlets was poorer than those of solid culture at the last phase in bioreactor culture, under the culture condition tested. The multiplication rate in TIS ofL. davidii was 1：17.23 whilst the rate was only 1：4.45 on solid culture, resulting larger bulbs than those in the solid culture. [ Conclusion] The TIS designed in our study could provide a potential mean for industrial production of plandets. However, the parameters vary greatly among different species, and it is to be optimized according to plant species.
文摘以芹菜、百合为主要原料,辅以柠檬酸、白砂糖,研制出口感优良、营养价值高的复合饮料。在单因素试验的基础上,通过正交试验设计,确定芹菜百合复合饮料的最佳配方为:百合汁和芹菜汁混合比例为1∶4(m L/m L),白砂糖6 g/100 m L,柠檬酸0.08 g/100 m L。通过试验,确定黄原胶0.03 g/100 m L与CMC-Na0.03 g/100 m L混合稳定剂为饮料的最佳稳定剂。此复合饮料营养丰富、风味独特,具有一定的应用前景。
