[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic h...[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.展开更多
Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous c...Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous cropping of Lanzhou lily(Lilium davidii var. unicolor). The changes of rhizosphere microbial biomass and diversity in interplanting and monoculturing systems were studied by using the Illumina Hi Seq sequencing technique. The contents and composition of lily root exudates were measured by gas chromatography–mass spectrometer(GC–MS). The intercropping results of Lanzhou lily showed:(1) There was no difference in the composition of the rhizosphere soil microbes at the phylum level, but the relative abundance of the microbes decreased; and the relative abundance of harmful fungi such as Fusarium sp. increased. The relative abundance of Pleosporales sp. and other beneficial bacteria were reduced. After OTU(operational taxonomic unit)clustering, there were some beneficial bacteria, such as Chaetomium sp., in the lily rhizosphere soil in the interplanting system that had not existed in the single-cropping system. We did not find harmful bacteria that had existed in the single-cropping systm in the rhizosphere soil of interplanting system. The above results indicated that the changes of relative abundance of soil fungi and bacteria in lily rhizosphere soil was not conducive to improving the ecological structure of rhizosphere soil microbes. At the same time, the microbial composition change is very complex—beneficial and yet inadequate at the same time.(2) Root exudates provide a matrix for the growth of microorganisms. Combined with the detection of root exudates, the decrease in the composition of the root exudates of the lily was probably the reason for the decrease of the relative abundance of microbes after intercropping. At the same time, the decrease of the relative content of phenolic compounds, which inhibit the growth of microorganisms, did not increase the relative content of rhizosphere soil microorganisms. Changes in amino acids and total sugars may be responsible for the growth of Fusarium sp.. The results showed that the intercropping pattern did not noticeably alleviate the obstacle to continuous cropping of Lanzhou lily, and the change of microbial biomass and diversity was even unfavorable. However, the emergence of some beneficial bacteria, the disappearance of harmful fungi, and other changes with intercropping are in favor of alleviation of obstacles to continuous cropping of Lanzhou lily.展开更多
Iron deficiency chlorosis of Lilium davidii var. unicolor is often the case in practice in alkaline soils of northwest region of China. It is difficult to control iron chlorosis because of high cost and short effectiv...Iron deficiency chlorosis of Lilium davidii var. unicolor is often the case in practice in alkaline soils of northwest region of China. It is difficult to control iron chlorosis because of high cost and short effective work time of conventional iron fertilizers. In this study, a 2-year field experiment was conducted to evaluate the effects of two slow-release fertilizers on the suppression of iron deficiency chlorosis, soil chemical properties, and the yield and quality of L. davidii var. unicolor. Results show that both coated slow-release iron fertilizers and embedded slow-release iron fertilizer effectively controlled iron-deficiency chlorosis. The application of slow-release iron fertilizers significantly increased plant height and chlorophyll content of L. davidii var. unicolor at different growth stages. Furthermore, coated iron fertilizer application significantly increased starch, protein, soluble sugar and vitamin C content of L. davidii var. unicolor, and it also significantly improved total amino acid content, with increases in essential amino acids(Trp, Leu, Lys, Phe, Val, and Thr contents) and in nonessential amino acids(Asp, Glu, Cit, Ihs, Acc, Ala, Pro, and Cys contents). It was concluded that application of coated slow-release iron fertilizer could be a promising option for suppression of iron deficiency chlorosis and deserves further study.展开更多
Changes in carbohydrate, phenols, free amino acids and endogenous hormones in middle scales of Lilium davidii var.unicolor bulbs during the 101 days storage at 2, 6 and 10°C in moist sawdust were studied. The res...Changes in carbohydrate, phenols, free amino acids and endogenous hormones in middle scales of Lilium davidii var.unicolor bulbs during the 101 days storage at 2, 6 and 10°C in moist sawdust were studied. The results indicated that asthe storage time increased and the storage temperature delined, contents of starch and abscisic acid (ABA) decreasedmarkedly, and the level of total soluble sugar, phenols, gibberellic acid (GA3) and indole acetic acid (IAA) increasedsharply. In the first 34 days, the contents of the above substances changed most actively. Correlation analysis revealedthat the increase in content of total soluble sugar and phenols was respectively caused by activation of amylase andphenylalanine ammonia-lyase (PAL). During the storage, the content of free amino acids decreased, and the level ofarginine was the highest and changed most remarkably. According to the results of stepwise regression analysis and pathanalysis, we could primarily conclude that the metabolism of carbohydrates, phenols and free amino acids in the middlescales may be mainly regulated by endogenous GA3.展开更多
为探究枯草芽孢杆菌(Bacillus subtilis)对兰州百合(Lilium davidii var. unicolor)生长发育及鳞茎代谢物的影响,以兰州百合为试验材料,设置CK(泥炭+珍珠岩)、TR(泥炭+珍珠岩+枯草芽孢杆菌)2组处理,采用灌根的方式施用枯草芽孢杆菌,观...为探究枯草芽孢杆菌(Bacillus subtilis)对兰州百合(Lilium davidii var. unicolor)生长发育及鳞茎代谢物的影响,以兰州百合为试验材料,设置CK(泥炭+珍珠岩)、TR(泥炭+珍珠岩+枯草芽孢杆菌)2组处理,采用灌根的方式施用枯草芽孢杆菌,观测不同时期(现蕾期、开花期、半枯期)兰州百合生长变化,并采用LC-MS法检测分析半枯期兰州百合鳞茎中差异代谢物。结果表明:与CK处理相比,TR处理对兰州百合的株高、茎粗、叶片数量、叶绿素含量、基生根数量、茎生根数量和鳞茎周径都有一定的促进作用。相较于CK处理,TR处理的株高和茎粗在现蕾期表现出显著差异(P<0.05),分别增加了20.06%和40.68%;叶片数量在现蕾期和开花期显著提高(P<0.05),达到CK处理的1.35倍和1.45倍;在开花期,TR处理的兰州百合鳞茎周径和茎生根数量相较于CK处理分别增加了15.87%和58.14%,差异显著(P<0.05)。在鳞茎代谢物分析中,通过CK组与TR组对比从兰州百合鳞茎中筛选出408种差异代谢物,富集的代谢途径有14条,其中差异代谢物主要富集在氨基酸代谢和合成通路。综上,配施枯草芽孢杆菌能够促进兰州百合生长发育,并影响鳞茎中营养物质代谢。展开更多
文摘[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.
基金funded by Lanzhou Branch of the Chinese Academy of Sciences institutional cooperation program(2BY52BI61)the Key program of Chinese Academy of Sciences(22Y622AM1)
文摘Both yield and quality of Lanzhou lily(Lilium davidii var. unicolor) are seriously affected by continuous cropping. We attempted to understand the effects of intercropping on the obstacles associated with continuous cropping of Lanzhou lily(Lilium davidii var. unicolor). The changes of rhizosphere microbial biomass and diversity in interplanting and monoculturing systems were studied by using the Illumina Hi Seq sequencing technique. The contents and composition of lily root exudates were measured by gas chromatography–mass spectrometer(GC–MS). The intercropping results of Lanzhou lily showed:(1) There was no difference in the composition of the rhizosphere soil microbes at the phylum level, but the relative abundance of the microbes decreased; and the relative abundance of harmful fungi such as Fusarium sp. increased. The relative abundance of Pleosporales sp. and other beneficial bacteria were reduced. After OTU(operational taxonomic unit)clustering, there were some beneficial bacteria, such as Chaetomium sp., in the lily rhizosphere soil in the interplanting system that had not existed in the single-cropping system. We did not find harmful bacteria that had existed in the single-cropping systm in the rhizosphere soil of interplanting system. The above results indicated that the changes of relative abundance of soil fungi and bacteria in lily rhizosphere soil was not conducive to improving the ecological structure of rhizosphere soil microbes. At the same time, the microbial composition change is very complex—beneficial and yet inadequate at the same time.(2) Root exudates provide a matrix for the growth of microorganisms. Combined with the detection of root exudates, the decrease in the composition of the root exudates of the lily was probably the reason for the decrease of the relative abundance of microbes after intercropping. At the same time, the decrease of the relative content of phenolic compounds, which inhibit the growth of microorganisms, did not increase the relative content of rhizosphere soil microorganisms. Changes in amino acids and total sugars may be responsible for the growth of Fusarium sp.. The results showed that the intercropping pattern did not noticeably alleviate the obstacle to continuous cropping of Lanzhou lily, and the change of microbial biomass and diversity was even unfavorable. However, the emergence of some beneficial bacteria, the disappearance of harmful fungi, and other changes with intercropping are in favor of alleviation of obstacles to continuous cropping of Lanzhou lily.
基金supported by China's National Natural Science Foundation (No. 41501043)by the "West Light" project of the Chinese Academy of Sciencesby the project of 60th Chinese postdoctorate science fund (No. 2016M602904)
文摘Iron deficiency chlorosis of Lilium davidii var. unicolor is often the case in practice in alkaline soils of northwest region of China. It is difficult to control iron chlorosis because of high cost and short effective work time of conventional iron fertilizers. In this study, a 2-year field experiment was conducted to evaluate the effects of two slow-release fertilizers on the suppression of iron deficiency chlorosis, soil chemical properties, and the yield and quality of L. davidii var. unicolor. Results show that both coated slow-release iron fertilizers and embedded slow-release iron fertilizer effectively controlled iron-deficiency chlorosis. The application of slow-release iron fertilizers significantly increased plant height and chlorophyll content of L. davidii var. unicolor at different growth stages. Furthermore, coated iron fertilizer application significantly increased starch, protein, soluble sugar and vitamin C content of L. davidii var. unicolor, and it also significantly improved total amino acid content, with increases in essential amino acids(Trp, Leu, Lys, Phe, Val, and Thr contents) and in nonessential amino acids(Asp, Glu, Cit, Ihs, Acc, Ala, Pro, and Cys contents). It was concluded that application of coated slow-release iron fertilizer could be a promising option for suppression of iron deficiency chlorosis and deserves further study.
基金This work was supported by the National Natural Science Foundation of China(30400307).
文摘Changes in carbohydrate, phenols, free amino acids and endogenous hormones in middle scales of Lilium davidii var.unicolor bulbs during the 101 days storage at 2, 6 and 10°C in moist sawdust were studied. The results indicated that asthe storage time increased and the storage temperature delined, contents of starch and abscisic acid (ABA) decreasedmarkedly, and the level of total soluble sugar, phenols, gibberellic acid (GA3) and indole acetic acid (IAA) increasedsharply. In the first 34 days, the contents of the above substances changed most actively. Correlation analysis revealedthat the increase in content of total soluble sugar and phenols was respectively caused by activation of amylase andphenylalanine ammonia-lyase (PAL). During the storage, the content of free amino acids decreased, and the level ofarginine was the highest and changed most remarkably. According to the results of stepwise regression analysis and pathanalysis, we could primarily conclude that the metabolism of carbohydrates, phenols and free amino acids in the middlescales may be mainly regulated by endogenous GA3.
文摘为探究枯草芽孢杆菌(Bacillus subtilis)对兰州百合(Lilium davidii var. unicolor)生长发育及鳞茎代谢物的影响,以兰州百合为试验材料,设置CK(泥炭+珍珠岩)、TR(泥炭+珍珠岩+枯草芽孢杆菌)2组处理,采用灌根的方式施用枯草芽孢杆菌,观测不同时期(现蕾期、开花期、半枯期)兰州百合生长变化,并采用LC-MS法检测分析半枯期兰州百合鳞茎中差异代谢物。结果表明:与CK处理相比,TR处理对兰州百合的株高、茎粗、叶片数量、叶绿素含量、基生根数量、茎生根数量和鳞茎周径都有一定的促进作用。相较于CK处理,TR处理的株高和茎粗在现蕾期表现出显著差异(P<0.05),分别增加了20.06%和40.68%;叶片数量在现蕾期和开花期显著提高(P<0.05),达到CK处理的1.35倍和1.45倍;在开花期,TR处理的兰州百合鳞茎周径和茎生根数量相较于CK处理分别增加了15.87%和58.14%,差异显著(P<0.05)。在鳞茎代谢物分析中,通过CK组与TR组对比从兰州百合鳞茎中筛选出408种差异代谢物,富集的代谢途径有14条,其中差异代谢物主要富集在氨基酸代谢和合成通路。综上,配施枯草芽孢杆菌能够促进兰州百合生长发育,并影响鳞茎中营养物质代谢。
