To facilitate the rapid production of lily （Liliumformolongi）, conditions favoring the germination of dormant lily seeds and in vitro regeneration from tissue cultttre were studied, using the germinating plumule as ...To facilitate the rapid production of lily （Liliumformolongi）, conditions favoring the germination of dormant lily seeds and in vitro regeneration from tissue cultttre were studied, using the germinating plumule as the explants. The optimal inducing conditions for dormant lily dry seeds to germinate were low tempera- ture （ 10 ℃ ） treatment for one hour followed by incubating at 18 ℃ with light illumination. After 8 days of incubation/induction, over 90% of the seeds were ger- minated. MS ＋ 6-BA 1.0 mg,/L ＋ NAA 0.5 mg/L was the most efficient medium formula in lily callus induction. MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.5 mg/L was the optimal medium for calli growth. 1/2MS ＋ NAA 1.0 mg/L was the medium gave the highest root inductive rate.展开更多
The optimal conditions of seeds germination for different cultivars was studied.The results showed that the suitable germination temperature varied with different cultivars.The optimal germination temperature was 20 ...The optimal conditions of seeds germination for different cultivars was studied.The results showed that the suitable germination temperature varied with different cultivars.The optimal germination temperature was 20 ℃ for early flowering cultivars ‘Raizan No.1’,while for medium flowering cultivar ‘Rinzan No.2’and late flowering cultivar ‘Raizan No.3’,the suitable temperature were 18 ℃ and 15 ℃ separately.During the process of seed germination,lighting time and light constitutes didn’t have significant effect on germination rate.The experiments showed that the germination rates of three cultivars decreased greatly when germination temperature was over 24 ℃,especially for ‘Raizan No.1’.So,different pre-chilling treatments of ‘Raizan No.1’ were taken,in order to solve this problem.The results indicated that germination at 30 ℃ was accelerated by pre-chilling treatment at 10 ℃ for more than 10 days,germination rate was more than 75%,and it was 85.22% by the same pretreatment for 20 days,the same as optimal germination temperature 20 ℃.Whereas pretreatment at 3 or 5 ℃ had no promotive effects at 30 ℃ germination condition,germination rate was less than 40%.In conclusion,pre-chilling treatmet at 10 ℃ for more than 10 days was recommended to germination of Lilium×formolongi under high temperature conditions.展开更多
文摘To facilitate the rapid production of lily （Liliumformolongi）, conditions favoring the germination of dormant lily seeds and in vitro regeneration from tissue cultttre were studied, using the germinating plumule as the explants. The optimal inducing conditions for dormant lily dry seeds to germinate were low tempera- ture （ 10 ℃ ） treatment for one hour followed by incubating at 18 ℃ with light illumination. After 8 days of incubation/induction, over 90% of the seeds were ger- minated. MS ＋ 6-BA 1.0 mg,/L ＋ NAA 0.5 mg/L was the most efficient medium formula in lily callus induction. MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.5 mg/L was the optimal medium for calli growth. 1/2MS ＋ NAA 1.0 mg/L was the medium gave the highest root inductive rate.
文摘The optimal conditions of seeds germination for different cultivars was studied.The results showed that the suitable germination temperature varied with different cultivars.The optimal germination temperature was 20 ℃ for early flowering cultivars ‘Raizan No.1’,while for medium flowering cultivar ‘Rinzan No.2’and late flowering cultivar ‘Raizan No.3’,the suitable temperature were 18 ℃ and 15 ℃ separately.During the process of seed germination,lighting time and light constitutes didn’t have significant effect on germination rate.The experiments showed that the germination rates of three cultivars decreased greatly when germination temperature was over 24 ℃,especially for ‘Raizan No.1’.So,different pre-chilling treatments of ‘Raizan No.1’ were taken,in order to solve this problem.The results indicated that germination at 30 ℃ was accelerated by pre-chilling treatment at 10 ℃ for more than 10 days,germination rate was more than 75%,and it was 85.22% by the same pretreatment for 20 days,the same as optimal germination temperature 20 ℃.Whereas pretreatment at 3 or 5 ℃ had no promotive effects at 30 ℃ germination condition,germination rate was less than 40%.In conclusion,pre-chilling treatmet at 10 ℃ for more than 10 days was recommended to germination of Lilium×formolongi under high temperature conditions.
