To analyze the bacterial communities in lime concretion black soil upon the incorporation of crop residues for two years in wheat-maize system, total DNA was directly extracted and PCR-amplified with the F357GC and R5...To analyze the bacterial communities in lime concretion black soil upon the incorporation of crop residues for two years in wheat-maize system, total DNA was directly extracted and PCR-amplified with the F357GC and R518 primers targeting the 16S rRNA genes of V3 region. The amplified fragments were analyzed by perpendicular DGGE. Analyzing of species richness index S and Shannon diversity index H revealed that there was a high diversity of soil bacterial community compositions among all treatments after incorporation of crop residues and fertilizing under field conditions. Eleven DGGE bands recovered were re-amplified, sequenced. Phylogenetic analysis of the representative DGGE fingerprints identified four groups of the prokaryotic communities in the soil by returning wheat residues and fertilizing under field conditions. The bacterial communities belonged to gamma proteobacterium, Cupriavidus sp, halophilic eubacterium, Acidobacterium sp, Sorangium sp, delta proteobacterium, Streptococcus sp and Streptococcus agalactiae were main bacterial communities. Principal Component Analysis (PCA) showed that there were the differences in DNA profiles among the six treatments. It showed that wheat residue returning, maize residue returning and fertilizing all can improve bacterial diversity in varying degrees. As far as improvement of bacterial diversity was concerned, wheat residue returning was higher than fertilizing, and fertilizing higher than maize residue returning.展开更多
用城市生活垃圾焚烧灰渣替代部分集料,按照9种不同的配比制备二灰灰渣碎石路面基层材料,进行路用性能试验。结果表明:与普通二灰碎石对比,在相同的二灰含量下,灰渣碎石的最大干密度降低8.8%,而最佳含水量增大38.4%;无侧限抗压强度最高可...用城市生活垃圾焚烧灰渣替代部分集料,按照9种不同的配比制备二灰灰渣碎石路面基层材料,进行路用性能试验。结果表明:与普通二灰碎石对比,在相同的二灰含量下,灰渣碎石的最大干密度降低8.8%,而最佳含水量增大38.4%;无侧限抗压强度最高可达1.03 M Pa;粉煤灰含量对材料强度影响较为显著,其中粉煤灰含量减少1%,无侧限抗压强度值降低21%;材料的干缩系数最大为67.2μ/%;石灰与粉煤灰含量越低,材料的干缩应变越小,平均干缩系数也越小,当二灰含量减少2.5%,平均干缩系数降低3.1%。二灰灰渣碎石材料满足基层的相关要求,灰渣可以为公路建设提供可持续的集料来源。展开更多
This study assesses the reactivity of four (04) different types of clayey earthen materials (Kamboinsé, Pabré, Saaba, and Kossodo) with Ca(OH)<sub>2</sub>, analytical lime (AL) versus lime residu...This study assesses the reactivity of four (04) different types of clayey earthen materials (Kamboinsé, Pabré, Saaba, and Kossodo) with Ca(OH)<sub>2</sub>, analytical lime (AL) versus lime residu (LR), in ambient conditions. The effect of the stabilization of the earth with the LR was also studied on the performances of compressed earth blocks (CEB). The lime content varied from 0% to 20% with respect to the mass of dry earth material. The analysis of the reactivity and physico-mechanical properties were respectively carried out on the solutions of the mixtures (earth + LR and earth + AL) and CEB (earth + LR). The mixtures were cured between 0 and 60 days at the ambient temperature of laboratory (30<span style="white-space:nowrap;">℃</span> ± 5<span style="white-space:nowrap;">℃</span>). The results showed that the reactivity of the clayey earth materials with AL is comparable to that with the LR. At the same time, this reactivity improved the physical and mechanical properties of stabilized CEB depending on the type of earth material, lime content and curing time. The maximum values of the dry compressive strength, observed for each material stabilized with 20% LR, are 6 and 7 MPa (Kamboinsé), 8.8 and 9.3 MPa (Pabré), 6 and 6.5 MPa (Saaba), 8.8 and 9.7 MPa (Kossodo) respectively at curing time of 28 and 45 days. The structural efficiency of CEB was also improved which implies that, in the current conditions, the stabilization of earth materials using LR allows to produce the CEB for potential applications in wall masonry.展开更多
文摘To analyze the bacterial communities in lime concretion black soil upon the incorporation of crop residues for two years in wheat-maize system, total DNA was directly extracted and PCR-amplified with the F357GC and R518 primers targeting the 16S rRNA genes of V3 region. The amplified fragments were analyzed by perpendicular DGGE. Analyzing of species richness index S and Shannon diversity index H revealed that there was a high diversity of soil bacterial community compositions among all treatments after incorporation of crop residues and fertilizing under field conditions. Eleven DGGE bands recovered were re-amplified, sequenced. Phylogenetic analysis of the representative DGGE fingerprints identified four groups of the prokaryotic communities in the soil by returning wheat residues and fertilizing under field conditions. The bacterial communities belonged to gamma proteobacterium, Cupriavidus sp, halophilic eubacterium, Acidobacterium sp, Sorangium sp, delta proteobacterium, Streptococcus sp and Streptococcus agalactiae were main bacterial communities. Principal Component Analysis (PCA) showed that there were the differences in DNA profiles among the six treatments. It showed that wheat residue returning, maize residue returning and fertilizing all can improve bacterial diversity in varying degrees. As far as improvement of bacterial diversity was concerned, wheat residue returning was higher than fertilizing, and fertilizing higher than maize residue returning.
文摘用城市生活垃圾焚烧灰渣替代部分集料,按照9种不同的配比制备二灰灰渣碎石路面基层材料,进行路用性能试验。结果表明:与普通二灰碎石对比,在相同的二灰含量下,灰渣碎石的最大干密度降低8.8%,而最佳含水量增大38.4%;无侧限抗压强度最高可达1.03 M Pa;粉煤灰含量对材料强度影响较为显著,其中粉煤灰含量减少1%,无侧限抗压强度值降低21%;材料的干缩系数最大为67.2μ/%;石灰与粉煤灰含量越低,材料的干缩应变越小,平均干缩系数也越小,当二灰含量减少2.5%,平均干缩系数降低3.1%。二灰灰渣碎石材料满足基层的相关要求,灰渣可以为公路建设提供可持续的集料来源。
文摘This study assesses the reactivity of four (04) different types of clayey earthen materials (Kamboinsé, Pabré, Saaba, and Kossodo) with Ca(OH)<sub>2</sub>, analytical lime (AL) versus lime residu (LR), in ambient conditions. The effect of the stabilization of the earth with the LR was also studied on the performances of compressed earth blocks (CEB). The lime content varied from 0% to 20% with respect to the mass of dry earth material. The analysis of the reactivity and physico-mechanical properties were respectively carried out on the solutions of the mixtures (earth + LR and earth + AL) and CEB (earth + LR). The mixtures were cured between 0 and 60 days at the ambient temperature of laboratory (30<span style="white-space:nowrap;">℃</span> ± 5<span style="white-space:nowrap;">℃</span>). The results showed that the reactivity of the clayey earth materials with AL is comparable to that with the LR. At the same time, this reactivity improved the physical and mechanical properties of stabilized CEB depending on the type of earth material, lime content and curing time. The maximum values of the dry compressive strength, observed for each material stabilized with 20% LR, are 6 and 7 MPa (Kamboinsé), 8.8 and 9.3 MPa (Pabré), 6 and 6.5 MPa (Saaba), 8.8 and 9.7 MPa (Kossodo) respectively at curing time of 28 and 45 days. The structural efficiency of CEB was also improved which implies that, in the current conditions, the stabilization of earth materials using LR allows to produce the CEB for potential applications in wall masonry.
