MiR-25-3p attenuates the proliferation of tongue squamous cell carcinoma cell line Tca8113

Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.

Detecting land subsidence near metro lines in the Baoshan district of Shanghai with multi-temporal interferometric synthetic aperture radar

Land subsidence is a major factor that affects metro line （ML） stability. In this study, an improved multi- temporal interferometric synthetic aperture radar （InSAR） （MTI） method to detect land subsidence near MLs is presented. In particular, our multi-temporal InSAR method provides surface subsidence measurements with high observation density. The MTI method tracks both point-like targets and distributed targets with temporal radar back- scattering steadiness. First, subsidence rates at the point targets with low-amplitude dispersion index （ADI） values are extracted by applying a least-squared estimator on an optimized freely connected network. Second, to reduce error propagation, the pixels with high-ADI values are classified into several groups according to ADI intervals and processed using a Pearson correlation coefficient and hierarchical analysis strategy to obtain the distributed targets. Then, nonlinear subsidence components at all point-like and distributed targets are estimated using phase unwrapping and spatiotemporal filtering on the phase residuals. The proposed MTI method was applied to detect land subsidence near MLs of No. 1 and 3 in the Baoshan district of Shanghai using 18 TerraSAR-X images acquired between April 21, 2008 and October 30, 2010. The results show that the mean subsidence rates of the stations distributed along the two MLs are -12.9 and -14.0 ram/year. Furthermore, three subsidence funnels near the MLs are discovered through the hierarchical analysis. The testing results demonstrate the satisfactory capacity of the proposed MTI method in providing detailed subsidence information near MLs.

Correcting the Contamination of Second-order Spectra:Improving HαMeasurements in Reverberation Mapping Campaigns

Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contamination of second-order spectra(SOS)which will introduce some undesirable uncertainties at the red side of the spectra.In this paper,we test the effect of SOS and propose a method to correct it in the time domain spectroscopic data using the simultaneously observed comparison stars.Based on the reverberation mapping(RM)data of NGC 5548 in2019,one of the most intensively monitored AGNs by the Lijiang 2.4 m telescope,we find that the scientific object,comparison star,and spectrophotometric standard star can jointly introduce up to~30%SOS for Grism 14.This irregular but smooth SOS significantly affects the flux density and profile of the emission line,while having little effect on the light curve.After applying our method to each spectrum,we find that the SOS can be corrected effectively.The deviation between corrected and intrinsic spectra is~2%,and the impact of SOS on time lag is very minor.This method makes it possible to obtain the HαRM measurements from archival data provided that the spectral shape of the AGN under investigation does not have a large change.

Discovery of a variable broad absorption line in the BL Lac object PKS B0138-097

We report the discovery of a broad absorption line （BAL） of - 10^4 km s^-1 in width in the previously known BL Lac object PKS 0138-097, which we tentatively identified as an Mg II BAL. This is the first detection of a BAL, which is sometimes seen in powerful quasars with high accretion rates, in a BL Lac object. The BAL was clearly detected in its spectra spanning two epochs at a high luminosity state taken in the Sloan Digital Sky Survey （SDSS）, while it disappeared in three SDSS spectra taken at a low luminosity state. The BAL and its variability pattern were also found in its historical multi-epoch spectra in the literature, but have been overlooked previously. In its high resolution radio maps, PKS 0138-097 shows a core plus a one- sided parsec-scale jet. The BAL variability can be interpreted as follows： The optical emission is dominated by the core in a high state and by the jet in a low state and the BAL material is located between the core and jet so that the BAL appears only when the core is shining. Our discovery suggests that outflows may also be produced in active galactic nuclei at a low accreting state.

Magneto-induced Line Broadening of Magneto-sensitive Lines in Solar Magnetized Atmospheres

We analyze the spectral line broadening of those magneto-sensitive lines in solar magnetized atmospheres. The broadening at the line wings is due to the increase of the effective width of energy levels involved in Zeeman splitting,and the broadening at the line core also originated in Zeeman splitting under the condition that the Zeeman components are mixed. Therefore, the magneto-induced or Zeeman broadening take effects on the whole line. The observed Stokes parameter data in a sunspot and outside it acquired by Solar Stokes Spectrum Telescope （S^3T） are analyzed for the demonstration of this mechanism, and the Zeeman broadening rates are calculated for Fe16302.5 under some assumptions.Our result shows that the broadening is increased as the magnetic field strength becomes stronger, but the rate of increase at the line core is decreased as the field strength increases, while the rate at the wing does not show such an obvious regularity. The broadening is more effective in the line core than in the wings.

THE DIFFERENTIATION OF HUMAN GASTRIC ADENOCAR-CINOMA CELL LINE MGc80-3 INDUCED BY DIBUTYRYL cAMP IN VITRO

For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells.

Line Broadening in a Limb Flare: Derivation of Macro-turbulent Velocity Fields

The line profiles of Ha in a limb flare on 1998 November 11 appear to be unusually broadened. It is considered that macro-turbulence (or macroscopic mass motions) may be one of the main causes. We use an inversion technique to extract the probability distribution of the line-of-sight velocity in the flare. There exist some differences between the velocity distributions deduced from Ha and from Ca II λ8542, which may be because the two lines depend differently on the temperature and velocity. Since the loop density is high, we obtain a rather short cooling time (several tens of seconds) from the hot X-ray loops to the cool loops visible in Ha. Possible origins of the large scale motions are discussed.

A search for evidence of small-scale inhomogeneities in dense cores from line profile analysis

In order to search for intensity fluctuations on the HCN（1-0） and HCO＋（1-0） line pro- files, which could arise due to possible small-scale inhomogeneous structure, long-term observations of high-mass star-forming cores S140 and S199 were carried out. The data were processed by the Fourier filtering method. Line temperature fluctuations that exceed the noise level were detected. Assuming the cores consist of a large number of randomly moving small thermal fragments, the total number of frag- ments is - 4 × 106 for the region with linear size - 0.1 pc in S140 and - 106 for the region with linear size - 0.3 pc in S 199. Physical parameters of fragments in S 140 were obtained from detailed modeling of the HCN emission in the framework of the clumpy cloud model.

ESTABLISHMENT OF A MAMMARY CANCER CELL LINE Ca 761-86 AND ITS BIOLOGICAL CHARACTERISTICS IN INBRED 615 MICE

A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes.

Strong optical and UV intermediate-width emission lines in the quasar SDSS J232444.80–094600.3:dust-free and intermediate-density gas at the skin of dusty torus?

Emission lines from the broad emission line region （BELR） and the narrow emission line region （NELR） of active galactic nuclei （AGNs） have been extensively studied. However, emission lines are rarely detected between these two regions. We present a detailed analysis of quasar SDSS J232444.80-094600.3 （SDSS J2324-0946）, which is remarkable for its strong intermediate-width emission lines （IELs） with FWHM ≈ 1800 km s^-1. The IEL component is present in different emission lines, including the permitted lines Lyαλ1216, CⅣ λ1549, semiforbidden line C Ⅲ] λ1909, and forbidden lines [OⅢ] ss4959, 5007. With the aid of photo-ionization models, we found that the IELs are produced by gas with a hydrogen density of nH - 10^6.2 -- 10^6.3 cm^-3, a distance from the central ionizing source of R - 35 - 50 pc, a covering factor of - 6%, and a dust-to-gas ratio of ≤ 4% that of the SMC. We suggest that the strong IELs of this quasar are produced by nearly dust-free and intermediate-density gas located at the skin of the dusty torus. Such strong IELs, which serve as a useful diagnostic, can provide an avenue to study the properties of gas between the BELR and the NELR.

Effect of DRB on the Biological Characteristics of Human Laryngeal Carcinoma Hep-2 Cell Line

In order to study the effect of 5, 6-Dichloro-l-13-D-ribofuranosyl-benzimidazole （DRB） on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry （FCM） and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose- and time-dependent manner. After being treated with 0, 10, 20, 40, 80 μmmol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was （0.68±0.19）%, （1.95±0.12）%, （8.51±0.26）%, （11.26±0.17）% and （14.99±0.32）%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 μmmol/L, and with the increase of DRB concentration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.

Relation between Starlight and Nebular Emission Lines of Star-Forming Galaxies

We present an exercise that intends to establish a relationship between the strength of nebular emission lines and optical stellar features in the spectrum of a galaxy. After accurately subtracting the stellar continuum and the underlying stellar absorption, we made reliable measurements of the emission lines of all the galaxies in the Sloan Digital Sky Survey Data Release 2 (SDSS DR2). More than 4000 star-forming galaxies with high S/N ratio of both the stellar spectrum and the emission lines are selected. These galaxy spectra are fitted with the 10 PCs of Yip et al., after all the emission line regions have been filtered out. We find that the flux of hydrogen Balmer emission lines, Ha and Hβ can be well recovered from the PCs, while the metal lines are not well reproduced. The fluxes of Ha and Hβ measured from the PC-reconstructed spectra and from the observed spectra agree well with an rms scatter of only - 0.1 dex. This result suggests that, with moderate spectral resolution and S/N ratio, the optical stellar spectrum of a galaxy can serve as an indicator of star formation rate.

SMA molecular line survey towards the massive star-forming region G10.6-0.4in W31complex

Line surveys of complex molecules with millimeter and sub-millimeter telescopes are important for probing the physical and chemical environments of massive star forming regions(MSFRs).We present a molecular line survey with the Submillimeter Array(SMA) in the frequency ranges of 220.3–222.3 GHz and 230.3–232.3 GHz toward G10.6-0.4, the brightest star forming core in the W31 complex. Ninety-nine transitions from 22 molecular species and their isotopologues are identified. The moment 0 images of typical molecules show a compact core which is concentrated at the continuum peak position. Based on the local thermodynamic equilibrium assumption, the molecular line data are modeled. The rotational temperatures of those molecular species range from 96 to 178 K and their column densities range from 2.0×1014to 3.7×1017cm-2. The observational data suggest that all complex molecules are located in a warm environment. Chemical environments of the molecules are discussed. We compared molecular abundances and gas temperatures in G10.6-0.4 with those in other MSFRs, and found that gas temperatures and fractional abundances of specific molecules in G10.6-0.4 are similar to the typical MSFR W51 North, suggesting that there are similar physical and chemical environments in these two MSFRs.

Effects of 4-(3-Chloro-Benzyl)-6,7-Dimethoxy-Quinazoline on Kinetics of P120-Catenin and Periplakin in Human Buccal Mucosa Squamous Carcinoma Cell Line

In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human buccal mucosa squamous cancer cell line BICR 10 treated with 3 nM tyrphostin. Growth of BICR 10 cells was inhibited by treatment with tyrphostin. Although changes were not observed in the expression of EGFR and p120-catenin, expression of Akt, Src and periplakin in BICR 10 treated with 3 nM tyrphostin tended to decrease. In addition, phosphorylation of EGFR, Akt and Src was inhibited by treatment with tyrphostin. On immunocytochemical staining, immunoreactions with phosphorylated EGFR, phosphorylated Akt and phosphorylated p120-catenin were weak in BICR 10 treated with tyrphostin. There was a slight immunocy to chemical reaction to periplakin in BICR 10 cells induced by tyrphostin. In conclusion, the decrease in phosphorylation in EGFR and p120-catenin by tyrphostin, following the decrease in Src or Akt phosphorylation, may inhibit expression of several growth factors associated with the proliferation and migration of cancer cells.

Relation between the “Double-Hump” Behavior in the Radio Band and the Broad-Line Luminosity for Blazars

The physics behind the spectral energy distribution （SED） of blazars remains open. We assembled 36 blazars to tackle the factors that control their SED. Now, many blazar spectra have the ＂double hump＂ feature in the radio and far-IR frequencies. For these a parameter, △, is created to characterize the behavior of the SED. We found a significant correlation between the broad-line luminosity （LBLR） and △. Because L BLR is an indicator of the accreting power of the source in blazars, we derived a linear correlation, △ oc M1/3.18, which suggests that the SED of blazars may depend on the accretion rate, like that of BL Lac objects. We also found a significant correlation between m and △ for a sample of 11 blazars （out of one of 36） with available black hole masses. This implies the Eddington accretion ratio may influence the shape of the SED of blazars.

Relations between the IR-UV-X-ray Continuum and Emission Lines for a Large Composite Sample of Narrow Line and Normal Seyfert 1 Galaxies

We report on our research on the UV, optical line parameters and the infrared through UV to soft X-ray continuum parameters for a composite sample of narrow line and normal Seyfert 1 galaxies. The strong correlations among the line width of HB, optical line strength of Fell and the soft X-ray slope are confirmed. We found no correlations between the UV line parameters, the equivalent widths of Lya and CIV and their ratio on one hand and the optical line parameters on the other. The UV and X-ray luminosities strongly correlate with the line widths of HB and the Fe II/HB ratio. No significant correlation is found between the infrared-soft X-my continuum slope aix and the line width of HB.

SDSS J143030.22-001115.1: A Misclassified Narrow-line Seyfert 1 Galaxy with Flat X-ray Spectrum

We used multi-component profiles to model the HE and [O Ⅲ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy （NLS1） in a sample of 150 NLS1 candidates selected from the Sloan Digital Sky Survey （SDSS）, Early Data Release （EDR）. After subtracting the HE contribution from narrow line regions （NLRs）, we found that its full width half maximum （FWHM） of broad H/3 line is nearly 2900km s^-1, significantly larger than the customarily adopted criterion of 2000 km s^-1. With its weak Fe II multiples, we believe that SDSS J143030.22-001115.1 should not be classified as a genuine NLS1. When we calculate the virial black hole masses of NLSls, we should use the Hβ linewidth after subtracting the NLR component.

Study on the method of optimal location on explosion events at near-source site

IntroductionEarthquake location is one of the inverse problems in seismology. The accurate earthquake source location is the basis of studies of earthquake images, the relation analysis between seismicity and tectonics, and the important parameters of earthquakes. It is also the basic data used to study the space distribution of aftershocks after a strong earthquake. The earthquake location accuracy mainly depends on reliability of the velocity models for the earth￠s crust, the reasonable design of the seismograph network, the accuracy of arrival time, and the location method used (ZHAO, 1983; ZHU, ZHAO, 1997; LI, et al, 1999). The reliability of the velocity model directly influences loca-tion results. The reasonable seismograph network design can improve the location accuracy; otherwise it will in-troduce ill-conditioned equations and large location error. If arrival time is not accurate, for example, if the seismic phase is wrongly identified or the time service is inaccurate, large arrival time error will be produced, and the location results and real hypocenters will be discordant. Different location algorithms adopted will produce differ-ent location errors. The residual criterion chosen has influence on location results. Besides, whether the event is correctly associated also influences the location quality. In practice, it can often be seen that large location devia-tion introduced by wrong event association.

Generation of selectable marker-free soft transgenic rice with transparent kernels by downregulation of SSSⅡ-2

The amylose content(AC) of rice endosperm starch varies from 0 to 35%,and is associated with rice cooking and eating quality.Soft rice has low AC,generally between 6% and 15%,and its eating quality is high whether it is consumed hot or cold.However,the appearance quality of current soft rice cultivars needs to be improved,especially opaque endosperm.Conventional genetic engineering has improved some agronomic traits of soft rice varieties,but not endosperm appearance.In the present study,a RNAi construct of the soluble starch synthase Ⅱ-2(SSSⅡ-2) and the hygromycin phosphotransferase(HPT) gene were introduced into an elite japonica rice variety,Kangtiaowuyunjing(KWY8) by co-transformation.Several selectable marker-free(SMF) transgenic lines were obtained,and SSSⅡ-2 expression was significantly downregulated in selected transgenic lines,resulting in lower AC of the endosperm.The physicochemical properties of the transgenic rice kernels,including gel consistency(GC) and rapid visco analyzer(RVA) profile,differed significantly from those of wild-type rice and were similar to those of a soft rice variety,Nanjing 46(NJ46).These findings indicate that the cooking,eating,and processing qualities of transgenic rice are comparable to those of NJ46.However,the transgenic rice endosperm retained a transparent appearance under low-moisture conditions.Thus,SMF SSSⅡ-2 RNAi rice provides a resource for breeding soft rice with transparent endosperm.

A Multi-Wavelength Study of the 3B/X1.2 Flare Observed on 2003 October 26

We report results from a multi-wavelength study of the 3B/X1.2 tworibbon disk flare （S15E44）, which was well observed by both ground-based and space-borne instruments. Two pairs of conjugate kernels - K1 and K4, and K2 and K3 - in the Ha images are identified. These kernels are linked by two different systems of EUV loops. K1 and K4 correspond to the two 17 GHz and 34 GHz microwave sources observed by the Nobeyama Radioheliograph （NoRH）, while K2 and K3 have no corresponding microwave sources. Optical spectroscopic observations suggest that all the four kernels are possible precipitating sites of non-thermal electrons. Thus the energy of electron deposited in K2 and K3 should be less than 100keV. Two-dimensional distributions of the full widths at half maximum （FWHM） of the Ha profiles and the line-of-sight （LOS） velocities derived from the Ca n 8542 A profiles indicate that the largest FWHM and LOS velocity tends to be located near the outer edges of Hα kernels, which is consistent with the scenario of current two-ribbon flare models and previous results. When non-thermal electron bombardment is present, the observed Hα and Ca II 8542 A profiles are similar to previous observational and theoretical results, while the He I 10830 A profiles are different from the theoretical ones. This puts some constraints on future theoretical calculation of the He I 10830 A line.