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Microstructure and Element Composition of Shell Plates of Liolophura japonica
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作者 CHEN Daohai1,2 1. College of Fisheries, Ocean University of China, Qingdao 266003, Shangdong, China 2. Life Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, Guangdong, China 《Wuhan University Journal of Natural Sciences》 CAS 2010年第2期176-184,共9页
The microstructure characteristics of shell plates of chiton Liolophura japonica Lischke were analyzed using environmental scanning electron microscopy (ESEM). The results show that the internal structure of the she... The microstructure characteristics of shell plates of chiton Liolophura japonica Lischke were analyzed using environmental scanning electron microscopy (ESEM). The results show that the internal structure of the shell plate of chiton Liolophurajaponica is composed of seven calcium layers which were crossed lamellar crystallites, homoge- neous structure, granular crystallites, and trabecular type crystallites. The element compositions of shell plates were analyzed using X-ray photoelectron spectroscopy (XPS). The results determined by XPS show that the surface chemical states of various layers are different. There are 13 elements (Na, O, N, C, S, P, Ca, C1, Si, A1, K, Fe, Mg) on the shell plate of chiton L. japonica. Among the 13 elements, 11 are on the outside pigment layer except K and S. Among seven layers of shell plate, there is the largest quantity of elements on the outside pigment layer, and the least quantity of elements is on layer C (Articulamentum auctorum). There are 7 elements on the layer C (Articulamentum auctorum). Be- sides calcium carbonate, there are some inorganic compounds on the shell plate, such as NaC1, MgO, A1203, silicate, sulfate and phosphate. There are some organic chemical components, such as carbohydrate, organic sulfide, and organic nitride on the shell plates. 展开更多
关键词 liolophura japonica environmental scanning electron microscope X-ray photoelectron spectroscopy (XPS) microstructure of shell plate
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基于DNA条形码对浙南岛屿日本花棘石鳖的遗传特征分析 被引量:3
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作者 吴晓雯 张华伟 +2 位作者 余海 彭欣 张永普 《海洋渔业》 CSCD 北大核心 2018年第3期265-278,共14页
采用COⅠ、16S rRNA两种分子标记对中国浙江南部沿海岛屿岩相潮间带日本花棘石鳖(Liolophura japonica)13个群体遗传特征和亲缘关系进行了探讨。结果显示:COⅠ基因片段长度为675 bp,16S rRNA基因长度为517 bp,A+T比例分别为62.5%和64.9%... 采用COⅠ、16S rRNA两种分子标记对中国浙江南部沿海岛屿岩相潮间带日本花棘石鳖(Liolophura japonica)13个群体遗传特征和亲缘关系进行了探讨。结果显示:COⅠ基因片段长度为675 bp,16S rRNA基因长度为517 bp,A+T比例分别为62.5%和64.9%。COⅠ基因总多态位点数为41个,单倍型多样性指数0.847,核苷酸多态性指数为0.006 01,平均核苷酸差异数为4.057 8;16S rRNA基因的总多态位点数为9个,单倍型多样性指数0.665,核苷酸多态性指数为0.001 69,平均核苷酸差异数为0.871 2。以Acanthopleura brevispinosa和A.granulata为外群构建了ML和BI系统发育树,其拓扑结构显示,基于COⅠ基因的日本花棘石鳖形成2个分支;基于16S rRNA基因的日本花棘石鳖群体仅聚合为一支。不同地理群体单倍型散乱分布,没有出现明显的地理结构和遗传谱系结构,表明各岛屿的日本花棘石鳖为近期分化并存在基因交流。 展开更多
关键词 日本花棘石鳖 COⅠ基因 16S RRNA基因 遗传特征
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