Matrine is a promising botanical antifungal;however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothid...Matrine is a promising botanical antifungal;however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothidea as a model strain. Matrine strongly inhibited mycelial growth of B. dothidea in a dose-dependent manner. Matrine-treated B. dothidea showed morphological and ultrastructural alterations, including shriveled hyphae, plasmolysis, and leakage of cytoplasm related to cell membrane deterioration. In addition, matrine caused significantly high conductivity and absorbance (260 nm) in extracellular matrices and low lipid contents in B. dothidea, indicating increased membrane permeability. Lipid peroxidation showed that matrine resulted in increased malondialdehyde content while enhancing the generation of reactive oxygen species and the activities of superoxide dismutase, catalase, and peroxidase. These results showed that matrine inhibited the mycelial growth of B. dothidea by enhancing cell membrane permeability via membrane lipid peroxidation.展开更多
Red Pine seedlings were grown in the potted soils with different acidity (pH=4.5,5.5,6.5,7.5,8.0) to investigate their physiological responses to soil pH,and futher to evaluate the soil pH feasibility. Dramatic physio...Red Pine seedlings were grown in the potted soils with different acidity (pH=4.5,5.5,6.5,7.5,8.0) to investigate their physiological responses to soil pH,and futher to evaluate the soil pH feasibility. Dramatic physiological responses of Red Pine seedlings were detected under various soil pH treatments. Under soil pH 5.5,the seedling foliages grew best,with the lowest malondialdehyde content,ion leakage and soluble substance content,and the highest carotinoid content and antioxidant enzyme activities;With soil pH 7.5 and 8.0 treatments,the seedling needles developed poorest,with greater membrane lipid peroxidation and lower antioxidant enzyme activities,and the greater cell membrane injury and soluble substance content. Results suggested that Red Pine seedlings was mostly adapt to soil with pH of 5.5,followed by soils with pH of 4.5 and 6.5,and could hardly adapted to soil with pH of 7.5 and higher.展开更多
基金funded by the Special Fund for Forest-Scientific Research in the Public Interest(No.201304403-4)the Fundamental Research Funds for the Central Universities(2572015AA25)
文摘Matrine is a promising botanical antifungal;however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothidea as a model strain. Matrine strongly inhibited mycelial growth of B. dothidea in a dose-dependent manner. Matrine-treated B. dothidea showed morphological and ultrastructural alterations, including shriveled hyphae, plasmolysis, and leakage of cytoplasm related to cell membrane deterioration. In addition, matrine caused significantly high conductivity and absorbance (260 nm) in extracellular matrices and low lipid contents in B. dothidea, indicating increased membrane permeability. Lipid peroxidation showed that matrine resulted in increased malondialdehyde content while enhancing the generation of reactive oxygen species and the activities of superoxide dismutase, catalase, and peroxidase. These results showed that matrine inhibited the mycelial growth of B. dothidea by enhancing cell membrane permeability via membrane lipid peroxidation.
文摘Red Pine seedlings were grown in the potted soils with different acidity (pH=4.5,5.5,6.5,7.5,8.0) to investigate their physiological responses to soil pH,and futher to evaluate the soil pH feasibility. Dramatic physiological responses of Red Pine seedlings were detected under various soil pH treatments. Under soil pH 5.5,the seedling foliages grew best,with the lowest malondialdehyde content,ion leakage and soluble substance content,and the highest carotinoid content and antioxidant enzyme activities;With soil pH 7.5 and 8.0 treatments,the seedling needles developed poorest,with greater membrane lipid peroxidation and lower antioxidant enzyme activities,and the greater cell membrane injury and soluble substance content. Results suggested that Red Pine seedlings was mostly adapt to soil with pH of 5.5,followed by soils with pH of 4.5 and 6.5,and could hardly adapted to soil with pH of 7.5 and higher.
