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银杏叶提取物对实验性糖尿病大鼠脑组织的保护作用 被引量:7
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作者 李剑敏 李旭升 +5 位作者 陈国荣 王芳 毛孙忠 吴步猛 方周溪 杨开颜 《中国临床药理学与治疗学》 CAS CSCD 2005年第8期935-939,共5页
目的:研究银杏叶提取物(extractofGinkgobi-loba,EGB)对糖尿病大鼠脑的保护作用及其机制。方法:选用雄性SD大鼠,随机分为糖尿病组、银杏叶组和正常对照组。应用链脲佐菌素(STZ)诱导制备大鼠糖尿病模型。银杏叶组腹腔注射银杏叶提取物注... 目的:研究银杏叶提取物(extractofGinkgobi-loba,EGB)对糖尿病大鼠脑的保护作用及其机制。方法:选用雄性SD大鼠,随机分为糖尿病组、银杏叶组和正常对照组。应用链脲佐菌素(STZ)诱导制备大鼠糖尿病模型。银杏叶组腹腔注射银杏叶提取物注射液,其余两组给予同容积的生理盐水,用药5周后,测定脑组织内皮素(ET)、丙二醛(MDA)、NO产物NO2-/NO3-的含量及超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的活性,以及血清中的血糖、胰岛素、ET水平,并作光镜及透射电镜观察。结果:糖尿病脑组织光镜下可见脑水肿、脑软化、神经细胞变性、白质脱髓鞘,透射电镜下可见神经元及神经胶质细胞内线粒体肿胀、嵴变短、神经纤维脱髓鞘、血脑屏障受损。EGB治疗后光镜、电镜下的病变明显减轻。与糖尿病组相比,银杏叶组大鼠血糖、血浆ET水平下降、胰岛素水平升高,脑组织内SOD活性明显升高,NOS活性、ET、MDA、NO2-/NO3-的含量明显下降。结论:EGB可能通过抗脂质过氧化及降低NO、ET水平,对糖尿病大鼠脑组织产生保护作用。 展开更多
关键词 银杏叶提取物 糖尿病 一氧化氮 脂质过氧化 内皮素
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香菇多糖对糖尿病大鼠巨噬细胞脂质过氧化和一氧化氮的影响
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作者 陈三妹 李剑敏 +2 位作者 陈国荣 毛孙忠 胡芸 《温州医学院学报》 CAS 2003年第5期325-326,共2页
目的:研究香菇多糖(LNT)对糖尿病大鼠巨噬细胞脂质过氧化作用和一氧化氮水平的影响。方法:SD大鼠分三组,正常对照组、糖尿病组、LNT治疗组,分别灌洗大鼠腹腔及肺泡巨噬细胞,并测定巨噬细胞内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GS... 目的:研究香菇多糖(LNT)对糖尿病大鼠巨噬细胞脂质过氧化作用和一氧化氮水平的影响。方法:SD大鼠分三组,正常对照组、糖尿病组、LNT治疗组,分别灌洗大鼠腹腔及肺泡巨噬细胞,并测定巨噬细胞内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、一氧化氮合酶(NOS)的活性及一氧化氮(NO)、丙二醛(MDA)的含量。结果:糖尿病大鼠腹腔及肺泡巨噬细胞内SOD、GSH-PX、NOS活性及NO含量下降,MDA含量增高,经LNT治疗后,SOD、GSH-PX、NOS活性及NO含量升高,MDA含量下降。结论:LNT可提高糖尿病大鼠巨噬细胞抗脂质过氧化作用及NO水平。 展开更多
关键词 糖尿病 实验性 香菇多糖 巨噬细胞 脂质过氧化作用 一氧化氮
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Lactobacillus plantarum B7 inhibits Helicobacter pylori growth and attenuates gastric inflammation 被引量:6
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作者 Chompoonut Sunanliganon Duangporn Thong-Ngam +1 位作者 Somying Tumwasorn Naruemon Klaikeaw 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第20期2472-2480,共9页
AIM:To determine the anti-Helicobacter property of Lactobacillus plantarum B7(L.plantarum)B7 supernatants in vitro and the protective effects of L.plantarum B7 on serum tumor necrosis factor-alpha(TNF-?),gastric malon... AIM:To determine the anti-Helicobacter property of Lactobacillus plantarum B7(L.plantarum)B7 supernatants in vitro and the protective effects of L.plantarum B7 on serum tumor necrosis factor-alpha(TNF-?),gastric malondialdehyde(MDA)level,apoptosis,and histopathology in Helicobacter pylori(H.pylori)-induced gastric inflammation in rats. METHODS:In vitro,the inhibition of H.pylori growth was examined using L.plantarum B7 supernatants at pH 4 and pH 7 and at the concentration of 1×,5×and 10×on plates inoculated with H.pylori.The inhibitory effect of H.pylori was interpreted by the size of the inhibition zone.In vitro,male Sprague-Dawley rats were randomly divided into four groups including group 1(control group),group 2(H.pylori infected group), group 3(H.pylori infected with L.plantarum B7 106 CFUs/mL treated group)and group 4(H.pylori infected with L.plantarum B7 1010 CFUs/mL treated group).One week after H.pylori inoculation,L.plantarum B7 106 CFUs/mL or 10 10 CFUs/mL were fed once daily to group 3 and group 4,respectively,for one week.Blood and gastric samples were collected at the end of the study. RESULTS:In vitro,at intact pH 4,mean inhibitory zone diameters of 8.5 mm and 13 mm were noted at concentrations of 5×and 10×of L.plantarum B7 supernatant disks,respectively.At adjusted pH 7, L.plantarum B7 supernatants at concentrations of 5 ×and 10×yielded mean inhibitory zone diameters of 6.5 mm and 11 mm,respectively.In the in vitro study, in group 2,stomach histopathology revealed mild to moderate H.pylori colonization and inflammation.The level of gastric MDA and epithelial cell apoptosis were significantly increased compared with group 1.The serum TNF-??level was significant decreased in group 3 compared with group 2(P<0.05).In addition,L.plantarum B7 treatments resulted in a significant improvement in stomach pathology,and decreased gastric MDA level and apoptotic epithelial cells. CONCLUSION:L.plantarum B7 supernatant inhibits H.pylori growth.This inhibition was dose-dependent and greater at pH 4.Moreover,L.plantarum B7 attenuated H.pylori-induced gastric inflammation. 展开更多
关键词 Apoptosis Gastric inflammation Helicobacter pylori Lactobacillus plantarum B7 lipid peroxi dation
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