AIM To evaluate the effects of chronic alcohol abuse on the mucosal permeability to lipopolysaccharide in the colon of rats. METHODS Escherichia coli lipopolysaccharide (LPS, 20mg/L) was injected into the colon...AIM To evaluate the effects of chronic alcohol abuse on the mucosal permeability to lipopolysaccharide in the colon of rats. METHODS Escherichia coli lipopolysaccharide (LPS, 20mg/L) was injected into the colon of chronic alcoholic rats ( n =10) which were supplied with Lieber diets every other day for 6 weeks. Before LPS injection and 5, 10, 20, 30 minutes after the injection, blood samples from the portal vein were obtained and contents of LPS in the blood were measured. The distribution of LPS in the colon tissues was observed with a confocal laser scanning microscope by immunofluorescent technique using the monoclonal antibody specific to the lipid A region of LPS. Normal rats were used as controls ( n =6). RESULTS Before LPS injection in the colon, LPS levels in the blood of portal vein of chronic alcoholic rats were significantly higher than those of normal controls (3 56ng/L±0 67ng/L, vs 2 45ng/L±0 15ng/L, P <0 01). At 5, 10, 20, 30 minutes after the injection of LPS, LPS contents were significantly higher than those before LPS injection (173 56ng/L±3 45ng/L, 154 78ng/L±0 57ng/L, 43 89ng/L±0 67ng/L, 45 38ng/L±0 89ng/L vs 3 56ng/L±0 67ng/L, P <0 01, respectively). Most mucosal cells showed strong positive reactions to LPS in the rats of chronic alcohol abuse, but no significant changes of LPS contents in blood from the portal vein and fluorescent reactions to LPS in mucosal cells of normal rats were found after LPS injection. CONCLUSION Chronic alcohol abuse resulted in a significant increase of permeability to LPS in colon mucosal cells in rats.展开更多
AIM To reveal the difference of Kupffer cells in the periportal and pericentral regions in the liver to uptake lipopolysaccharides (LPS) injected by the portal vein. METHODS Male Wistar rats were divided into two g...AIM To reveal the difference of Kupffer cells in the periportal and pericentral regions in the liver to uptake lipopolysaccharides (LPS) injected by the portal vein. METHODS Male Wistar rats were divided into two groups: normal control group ( n =6) and GdCl 3 treated group ( n =8). Sixteen hours before the experiment, rats of GdCl 3 treated group were injected GdCl 3 by the tail vein to eliminate the Kupffer cell function in the periportal region selectively. LPS at a dose of 20μg/100g body weight was injected to rats of both groups by the portal vein. Zero, 2, 5, 10, 30, 60 minutes after LPS injection, liver samples were obtained and distribution of LPS in Kupffer cells was observed by the immunofluorescent method using a monoclonal antibody specific to LPS with a confocal laser scanning microscope. RESULTS In normal control group, positive reactions to LPS were found in Kupffer cells in the periportal region with the peak at 2 minutes after LPS injection. Kupffer cells in the pericentral region showed the peak at 5 minutes after LPS injection, but its fluorescent intensity to LPS at the peak time in the cytoplasm was significantly lower than that of Kupffer cells in the pericentral region at the peak point. In GdCl 3 treated group, Kupffer cells in the pericentral region showed the peak at 2 minutes after LPS injection, and its fluorescent intensity to LPS showed no significant difference from that of the normal control rats at the peak point. No significant changes of fluorescent intensities to LPS were found in Kupffer cells in the periportal region at various time points after LPS injection in GdCl 3 treated rats. CONCLUSION Kupffer cells in the periportal and pericentral regions showed difference to uptake LPS in the portal vein.展开更多
文摘AIM To evaluate the effects of chronic alcohol abuse on the mucosal permeability to lipopolysaccharide in the colon of rats. METHODS Escherichia coli lipopolysaccharide (LPS, 20mg/L) was injected into the colon of chronic alcoholic rats ( n =10) which were supplied with Lieber diets every other day for 6 weeks. Before LPS injection and 5, 10, 20, 30 minutes after the injection, blood samples from the portal vein were obtained and contents of LPS in the blood were measured. The distribution of LPS in the colon tissues was observed with a confocal laser scanning microscope by immunofluorescent technique using the monoclonal antibody specific to the lipid A region of LPS. Normal rats were used as controls ( n =6). RESULTS Before LPS injection in the colon, LPS levels in the blood of portal vein of chronic alcoholic rats were significantly higher than those of normal controls (3 56ng/L±0 67ng/L, vs 2 45ng/L±0 15ng/L, P <0 01). At 5, 10, 20, 30 minutes after the injection of LPS, LPS contents were significantly higher than those before LPS injection (173 56ng/L±3 45ng/L, 154 78ng/L±0 57ng/L, 43 89ng/L±0 67ng/L, 45 38ng/L±0 89ng/L vs 3 56ng/L±0 67ng/L, P <0 01, respectively). Most mucosal cells showed strong positive reactions to LPS in the rats of chronic alcohol abuse, but no significant changes of LPS contents in blood from the portal vein and fluorescent reactions to LPS in mucosal cells of normal rats were found after LPS injection. CONCLUSION Chronic alcohol abuse resulted in a significant increase of permeability to LPS in colon mucosal cells in rats.
文摘AIM To reveal the difference of Kupffer cells in the periportal and pericentral regions in the liver to uptake lipopolysaccharides (LPS) injected by the portal vein. METHODS Male Wistar rats were divided into two groups: normal control group ( n =6) and GdCl 3 treated group ( n =8). Sixteen hours before the experiment, rats of GdCl 3 treated group were injected GdCl 3 by the tail vein to eliminate the Kupffer cell function in the periportal region selectively. LPS at a dose of 20μg/100g body weight was injected to rats of both groups by the portal vein. Zero, 2, 5, 10, 30, 60 minutes after LPS injection, liver samples were obtained and distribution of LPS in Kupffer cells was observed by the immunofluorescent method using a monoclonal antibody specific to LPS with a confocal laser scanning microscope. RESULTS In normal control group, positive reactions to LPS were found in Kupffer cells in the periportal region with the peak at 2 minutes after LPS injection. Kupffer cells in the pericentral region showed the peak at 5 minutes after LPS injection, but its fluorescent intensity to LPS at the peak time in the cytoplasm was significantly lower than that of Kupffer cells in the pericentral region at the peak point. In GdCl 3 treated group, Kupffer cells in the pericentral region showed the peak at 2 minutes after LPS injection, and its fluorescent intensity to LPS showed no significant difference from that of the normal control rats at the peak point. No significant changes of fluorescent intensities to LPS were found in Kupffer cells in the periportal region at various time points after LPS injection in GdCl 3 treated rats. CONCLUSION Kupffer cells in the periportal and pericentral regions showed difference to uptake LPS in the portal vein.
