The cross resistance of four resistant strains of Liposcelis bostrychophila Badonnel, an important stored-product insect pest, was investigated. These four strains included the HCO2-R (hypercarbia-resistant strain),...The cross resistance of four resistant strains of Liposcelis bostrychophila Badonnel, an important stored-product insect pest, was investigated. These four strains included the HCO2-R (hypercarbia-resistant strain), HCLO-R (hypoxia and hypercarbia resistant strain), DDVP-R (DDVP-resistant strain) and PH3-R (PH3-resistant strain). The results indicated that there were cross-resistances between CA and insecticides, and the quantities of the cross-resistance were different. The cross-resistance factor (RF) of HCO2-R to hypoxia and hypercarbia was 3.2458, whilst, that of HCLO-R to hypercarbia was 1.8280. The RF of DDVP-R to PH3 was 3.9614, whilst, that of PH3-R to DDVP was 2.7852. The RF values of DDVP- R and PH3-R to hypercarbia were 1.3550 and 1.1816, respectively. However, the RF of HCO2- R to DDVP was 2.1372. There also was a low cross-resistance between DDVP-R and HCO2-R. The RF of HCO2-R to PH3 was 3.3698. This suggested that the insects resistant to high CO2 concentration atmosphere would develop significant resistance to PH3. However, the insects resistant to PH3 remain sensitive to hypercarbia atmosphere. Both DDVP-R and PH3- R developed resistance to high CO2 treatment. There was a low cross-resistance between DDVP-R and HCLO-R. The insects resistant to hypoxia and hypercarbia were very sensitive to DDVP. There also was cross-resistance between HCLO-R and PH3-R, but the resistance of PH3-R to hypoxia and hypercarbia was lower than that of HCLO-R to PH3. The difference of the overlapping and separate values indicated that there were differences in the intercross-resistance of four resistant strains.展开更多
Among the diverse soil bacteria, plant growth promoting rhizobacteria (PGPR) mark an important role in enhancing plant growth through a range of beneficial functions. This is mainly achieved by effective rhizosphere c...Among the diverse soil bacteria, plant growth promoting rhizobacteria (PGPR) mark an important role in enhancing plant growth through a range of beneficial functions. This is mainly achieved by effective rhizosphere colonization by PGPR. Biofilm development by PGPR is considered as a survival strategy over the planktonic mode of growth under stress and natural conditions. Since the performance of microbial inoculants under field conditions is not always consistent due to various biotic and abiotic factors affecting survival, colonization and functions. Therefore, the rhizobacteria with efficient colonization ability and exhibiting multiple PGP traits are expected to perform better. We hypothesized that the biofilm forming ability of PGPR on plant root will be an added advantage to rhizosphere colonization. Therefore, we have selected a promising isolate of PGPR through random screening programme from rhizoplane of wheat (Triticum aestivum). The selection was based on biofilm development ability, multifarious PGP activities (production of indole acetic acid, sidero-phore, phosphate solubilization, hydrogen cyanide, ammonia production and biocontrol activity) and tolerance to salinity and heavy metals. The selected isolate was identified by 16 s rRNA partial gene sequencing as Pseudomonas entomophila-FAP1. The strain FAP1 formed strong biofilm in microtitre plate, glass surface as well as on the roots of wheat seedlings. Biofilm forming capacity of the FAP1 was characterized by scanning electron microscopy and confocal laser scanning microscopy. FAP1 exhibited biofilm-related traits such as the production of exopolysaccharides, EPS (1501.33 ± 1.08 μg ml-1), alginate (212.81 ± 1.09 μg ml-1), swarming motility (22 ± 1.36 mm), swimming motility (31 ± 2.12 mm) and cell surface hydrophobicity (63%). Rhizosphere colonization by FAP1 was found 7.5 Log CFU g-1 of soil comparable with rhizoplane colonization (7.2 Log CFU g-1 of root). Therefore, biofilm formation on plant roots by promising PGPR may be included as an additional criterion to select a better rhizosphere colonizer. Further, study with mutant deficient in biofilm should be developed for comparative study to explore the exact contribution of biofilm in root colonization under natural soil-plant system.展开更多
旨在建立嗜虫书虱的实时荧光定量PCR分析方法,为嗜虫书虱基因的定量分析提供技术支持。利用RT-PCR方法,从嗜虫书虱体内克隆获得了β-actin基因cDNA片段(GenBank登录号:FJ041117),该基因片段长度为822 bp,编码273个氨基酸残基(从第3个碱...旨在建立嗜虫书虱的实时荧光定量PCR分析方法,为嗜虫书虱基因的定量分析提供技术支持。利用RT-PCR方法,从嗜虫书虱体内克隆获得了β-actin基因cDNA片段(GenBank登录号:FJ041117),该基因片段长度为822 bp,编码273个氨基酸残基(从第3个碱基开始编码)。根据此β-actin基因的序列设计引物,建立了基于SYBR Green I染料技术的实时荧光定量PCR方法。建立的嗜虫书虱β-actin实时荧光定量PCR法扩增效率高、检测范围广、检测周期短,为β-actin作为内参基因进行嗜虫书虱功能基因的定量分析奠定了基础。展开更多
基金funded in part by the Nationa1 Natura1 Science Foundation(38800017)of ChinaFok Ying-Tung Education Foundation(71022).
文摘The cross resistance of four resistant strains of Liposcelis bostrychophila Badonnel, an important stored-product insect pest, was investigated. These four strains included the HCO2-R (hypercarbia-resistant strain), HCLO-R (hypoxia and hypercarbia resistant strain), DDVP-R (DDVP-resistant strain) and PH3-R (PH3-resistant strain). The results indicated that there were cross-resistances between CA and insecticides, and the quantities of the cross-resistance were different. The cross-resistance factor (RF) of HCO2-R to hypoxia and hypercarbia was 3.2458, whilst, that of HCLO-R to hypercarbia was 1.8280. The RF of DDVP-R to PH3 was 3.9614, whilst, that of PH3-R to DDVP was 2.7852. The RF values of DDVP- R and PH3-R to hypercarbia were 1.3550 and 1.1816, respectively. However, the RF of HCO2- R to DDVP was 2.1372. There also was a low cross-resistance between DDVP-R and HCO2-R. The RF of HCO2-R to PH3 was 3.3698. This suggested that the insects resistant to high CO2 concentration atmosphere would develop significant resistance to PH3. However, the insects resistant to PH3 remain sensitive to hypercarbia atmosphere. Both DDVP-R and PH3- R developed resistance to high CO2 treatment. There was a low cross-resistance between DDVP-R and HCLO-R. The insects resistant to hypoxia and hypercarbia were very sensitive to DDVP. There also was cross-resistance between HCLO-R and PH3-R, but the resistance of PH3-R to hypoxia and hypercarbia was lower than that of HCLO-R to PH3. The difference of the overlapping and separate values indicated that there were differences in the intercross-resistance of four resistant strains.
文摘Among the diverse soil bacteria, plant growth promoting rhizobacteria (PGPR) mark an important role in enhancing plant growth through a range of beneficial functions. This is mainly achieved by effective rhizosphere colonization by PGPR. Biofilm development by PGPR is considered as a survival strategy over the planktonic mode of growth under stress and natural conditions. Since the performance of microbial inoculants under field conditions is not always consistent due to various biotic and abiotic factors affecting survival, colonization and functions. Therefore, the rhizobacteria with efficient colonization ability and exhibiting multiple PGP traits are expected to perform better. We hypothesized that the biofilm forming ability of PGPR on plant root will be an added advantage to rhizosphere colonization. Therefore, we have selected a promising isolate of PGPR through random screening programme from rhizoplane of wheat (Triticum aestivum). The selection was based on biofilm development ability, multifarious PGP activities (production of indole acetic acid, sidero-phore, phosphate solubilization, hydrogen cyanide, ammonia production and biocontrol activity) and tolerance to salinity and heavy metals. The selected isolate was identified by 16 s rRNA partial gene sequencing as Pseudomonas entomophila-FAP1. The strain FAP1 formed strong biofilm in microtitre plate, glass surface as well as on the roots of wheat seedlings. Biofilm forming capacity of the FAP1 was characterized by scanning electron microscopy and confocal laser scanning microscopy. FAP1 exhibited biofilm-related traits such as the production of exopolysaccharides, EPS (1501.33 ± 1.08 μg ml-1), alginate (212.81 ± 1.09 μg ml-1), swarming motility (22 ± 1.36 mm), swimming motility (31 ± 2.12 mm) and cell surface hydrophobicity (63%). Rhizosphere colonization by FAP1 was found 7.5 Log CFU g-1 of soil comparable with rhizoplane colonization (7.2 Log CFU g-1 of root). Therefore, biofilm formation on plant roots by promising PGPR may be included as an additional criterion to select a better rhizosphere colonizer. Further, study with mutant deficient in biofilm should be developed for comparative study to explore the exact contribution of biofilm in root colonization under natural soil-plant system.
文摘旨在建立嗜虫书虱的实时荧光定量PCR分析方法,为嗜虫书虱基因的定量分析提供技术支持。利用RT-PCR方法,从嗜虫书虱体内克隆获得了β-actin基因cDNA片段(GenBank登录号:FJ041117),该基因片段长度为822 bp,编码273个氨基酸残基(从第3个碱基开始编码)。根据此β-actin基因的序列设计引物,建立了基于SYBR Green I染料技术的实时荧光定量PCR方法。建立的嗜虫书虱β-actin实时荧光定量PCR法扩增效率高、检测范围广、检测周期短,为β-actin作为内参基因进行嗜虫书虱功能基因的定量分析奠定了基础。
