Quality Standards of Carpesium lipskyi

[Objectives]The paper was to establish the quality standard of Carpesium lipskyi,and to provide a scientific reference for its quality control and rational development and utilization.[Methods]Morphological identification,microscopic identification and thin layer chromatography(TLC)identification of the herbal samples were performed.The contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extract of the herbal samples were determined,and their total flavonoid content was determined by UV-spectrophotometry.[Results]The average contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of 10 batches of C.lipskyi were 10.38%-12.27%,8.03%-14.72%,1.01%-3.06%and 20.84%-26.82%,respectively.The total flavonoids content ranged from 4.60%to 5.63%.[Conclusions]The established standard can provide a reference for the quality control of C.lipskyi.The tentative regulations stipulate that the moisture content of C.lipskyi shall not exceed 13.0%;the total ash content shall not exceed 15.0%;the acid-insoluble ash content shall not exceed 4.0%;the alcohol-soluble extract shall not be less than 16.0%;and the total flavonoids shall not be less than 3.0%.

New Sesquiterpene Lactones from Carpesium Lipskyi

ＮｏｐｒｅｖｉｏｕｓｗｏｒｋｏｎｔｈｅｃｈｅｍｉｃａｌｃｏｎｓｔｉｔｕｅｎｔｓｏｆＣａｒｐｅｓｉｕｍｌｉｐｓｋｙｉ（ｆａｍｉｌｙ：Ｃｏｍｐｏｓｉｔａｅ），ｕｓｅｄａｓｆｏｌｋｍｅｄｉｃｉｎｅ，ｓｉｎｃｅａｎｃｉｅｎｔｔｉｍｅｓ，ｆｏｒｒｅｌｉｅｖｉｎ...

基于植物代谢组学混合物氢谱测定概念快速发现先导化合物的方法学研究

目的建立从复杂植物样品中快速发现有意义的先导化合物的研究方法。方法混合组分测定氢谱,用混合物氢谱测定获得的线索指导进一步分离。结果以基于植物代谢组学概念的混合物氢谱测定方法为指导,从高原天名精中快速分离得到有价值的倍半萜类化合物,避免了天然产物化学经典分离方法的大量、无意义的重复实验,提高了发现先导化合物的效率。结论基于植物代谢组学混合物氢谱测定指导下的分离是一种高效发现先导化合物的新方法。

高原天名精挥发油成分的GC-MS分析

[目的]分析高原天名精全草挥发油的化学成分。[方法]采用水蒸气蒸馏法提取挥发油,用GC-MS联用技术对挥发油成分进行分离鉴定,采用面积归一化法确定各成分的相对含量。[结果]共鉴定出49个成分,占总峰面积的99.55%。其主要化学成分为马鞭烯醇、3,4-二乙氧基苯乙腈、5-甲基-2-烯丙基苯酚、6S-2,3,8,8-四甲基三环[5.2.2.0(1,6)]十一-2-烯、环氧柏木烷、香树烯氧化物(1)、2,3,5,9-四甲基-三环[6.3.0.0(1,5)]十一碳-2-烯-4-酮、植酮。[结论]该研究为高原天名精的深入开发利用提供一定的科学依据。

高原天名精化学成分研究

目的:对藏药高原天名精Carpesium lipskyi甲醇提取物化学成分进行分离鉴定。方法:利用普通硅胶柱色谱方法进行分离、纯化,并经超导核磁共振(NMR)、质谱(MS)、红外(IR)等现代波谱技术确定其结构。结果:从高原天名精的甲醇室温提取物乙酸乙酯部位获得6个化合物,其结构被确定为β-谷甾醇(I),豆甾醇(II),二十二烷醇(III),胡萝卜苷(IV),5-羟基-3,6,7,4’-四甲氧基黄酮(V),异鼠李素-3-O-β-D-葡萄糖苷(VI)。结论:6个化合物均为首次从该植物中分离得到。