Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture

The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.

Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and Metabolites Regulated in Treated Cancer Cell Lines

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

Investigation of the Acceptability of Cultured Meat in University Students

Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

Historiography and Scientific Research of Religious Buildings of the Cucuteni-Trypillian Culture

The purpose of this article is to reveal the historiography and current state of research related to the cult buildings of the Eneolithic period of the Cucuteni-Trypillian cultural community.The article describes the historical path of archaeological discoveries,from the first Trypillian sanctuaries to the discovery of the largest Nebelivka temple complex in Ukraine in 2012.At the same time,the work partially raises the issue of religious beliefs of the ancient farmers of Central Europe in connection with the discovery of their sacred buildings.Since special scientific works have not comprehensively addressed this issue and are still fragmentary in nature,it is important at the present stage of research to make some coverage and systematization of existing materials on this issue.

Comparative Study of the Widal Test against Stool Culture in the Diagnosis of Suspected Cases of Typhoid Fever in Some Low Income Communities in the Adamawa Region of Cameroon

Introduction: Infectious diseases constitute a major concern of public health in developing countries. Facilities and well trained staff have been shown to be one of the major obstacles in the rapid and quality diagnosis of these diseases. As such, we carried out an analysis to compare the Widal test and stool culture to identify febrile patients with Salmonella infection. Method: A cross sectional study was conducted to diagnose salmonella infection with out-patients who demonstrated signs of salmonella infection. Serum was harvested from blood collected from 368 (Vina = 234, Mayo Banyo 65, and Djerem = 69) patients accompanied by stool, Widal test was conducted on the spot and stool was taken to a reference laboratory for culture using standard microbiological methods, sociological set up was calculated in percentages, prevalence was calculated using excel while statistical difference was calculated using SPSS. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated to compare the Widal test against stool culture. Results: A total of 368 (50.8% females and 49.2% males) participants took part in the survey. Salmonella prevalence (66.24%) in stool culture in the Vina division was significantly different (p 0.05). The sensitivity,specificity, PPV, and NPV of slide agglutination test against stool culture varied from different areas (Vina: 51.6%, 73.62%, 79.21% and 43.61%;Mayo Banyo: 60.53%, 77.78%, 79.31% and 58.33%;Djerem: 53.18%, 83.73% 73.91% and 67.39%) respectively. Slide agglutination test has a fair agreement with the stool culture (kappa, Vina = 0.202;Mayo Banyo = 0.37 and Djerem = 0.38). Conclusion: Generally, in the three areas of study, the Widal test had a fair correlation with the stool culture;This means the Widal test should not be used alone but in combination with stool culture in the detection of salmonella infections.

The effect of"rural cultural auditorium"on the revitalization of rural culture:A case study of villages A and B in Zhejiang Province

The construction of rural cultural auditorium is conducive to gathering local popularity,excavating history and continuing civilization,and it has become a powerful driving force for cultural revitalization in rural revitalization.However,the development of"Rural Cultural Auditorium"encountered many problems so far.From the perspective of rural cultural revitalization,and under the background of the new socialist rural cultural construction in China,this paper takes the rural cultural auditorium as the topic.The field questionnaire survey and semi-structured interview were constructed in Village A and Village B of Zhejiang Province,.SPSS software is used to analyze the collected questionnaire data.On the basis of combing related concepts and theories,it summs up the problems of"rural cultural auditorium",such as the existence of cultural auditorium,low participation rate of cultural auditorium activities,poor information communication,etc.Through visiting and investigating,going deep into the local area,according solutions are put forward such as increasing content diversity,paying attention to publicity and increasing interaction,so as to promote the healthy and sustainable development of rural cultural auditorium.It is hoped that this paper will explore the significance of the construction of rural cultural auditorium to the revitalization of rural culture in China,and put forward ideas on how to further build rural cultural auditorium,so as to provide reference for rural cultural construction services.

Integrating Chinese Culture Into Language Curriculum:Teaching Chinese Culture to International Students in China

This paper explores the integration of Chinese culture into language education for German students at the University of Shanghai for Science and Technology(USST).Focusing on USST’s Chinese curriculum and pedagogical strategies,the study emphasizes the importance of cultural immersion,experiential learning,and authentic materials.Drawing on Byram’s Intercultural Communicative Competence(ICC)model,the Cultural Studies Approach,and Task-Based Language Teaching(TBLT),the paper presents a case study on incorporating Chinese calligraphy into regular classes.This hands-on approach not only enriches cultural understanding but also enhances language skills.The findings stress the need for tailored,multifaceted pedagogical approaches to prepare international students for cross-cultural interactions in a globalized context.

Contextualized Textiles Chinese anthropologist explores the fabric of Malay culture

Yi Village in Kelantan State,Malaysia,is known for its batik production.Batik is a wax-dyed fabric that evolved from sarong garments.To make batik,artisans first use melted wax to draw various lines and patterns on white or pre-dyed fabric,then fill in different colors between the lines.Then,the fabric is placed under the sun to dry.

Inclusiveness Nurtures Diversity Exchange of ethnic culture brings stability and prosperity in a multiethnic country

In China,unity fosters diversity,and the diversity enriches the unity.The process of collision and integration was not aimed at eliminating each other but at enhancing each other,resulting in the emergence of a more inclusive civilization.

Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough

The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.

High-quality Development of the Pearl River Night Tour in the Context of Deep Integration of Culture and Tourism

Urban night tourism is a new form of urban tourism development and an important driving force for the prosperity of urban night economy.As the most prestigious business card among many night tour projects in Guangzhou,the Pearl River water night tour has contributed to the development of Guangzhou s night economy,which cannot be underestimated.However,in the process of rapid development,there are also problems such as diversified operators,insufficient integration of intangible cultural elements,excessive commercial attributes,issues of tourism facilities and service facilities.Under the new stage of integrated development of culture and tourism,how to use mutual promotion of culture and tourism in the Pearl River night tour to seek innovative paths for high-quality development in the future is the main problem expected to be solved in this paper.Therefore,based on the perspective of culture and tourism integration,this paper analyzes the general situation and development status of the Pearl River night tour,and proposes five development strategies:the overall planning of culture and tourism integration,the deep integration of intangible cultural elements,the balance of commercial attributes and tourism attributes,the high integration of culture,business,tourism and education,and the creation of tourism atmosphere,in order to provide inspiration for the healthy,orderly and high-quality development of the Pearl River night tour.

Polar Models of Culture-Regressivism Versus Progressivism

The culture wars that simmer within any nation may have been escalating recently;but regardless of their national settings or milieus,many of these wars are informed by two opposing paradigms of culture.This paper analyzes two of these leading paradigms,designated here as Regressivism and Progressivism.Other theorists have long chronicled the differing strengths and weaknesses of these paradigms,taking the former as expressing more conservative,traditional,and nationalistic values,and the latter as expressing more liberal,pluralistic,and cosmopolitan values.Going beyond these perennial distinctions,I argue that Progressivism is more benign and beneficial than the former-by meeting basic human needs better and supporting more effective adaptation to changing exigencies.I also argue that Progressivism does not express merely subjective or relativistic preferences and values,but objectively preferable and quantifiable ones,that benefit not only our personal lives more but also our global village and communal lives more.

Traumatized Interpretations of Female Characters in American Southern Culture in The Glass Menagerie

Tennessee Williams is the most famous Southern playwright in the history of 20th century American literature,and southern identity is a thread that runs throughout Williams’work.The Glass Menagerie is one of his highly representative dramatic works.The play tells the story of how an ordinary family in deep identity crisis escapes the harsh reality and cultural trauma during the Great Depression in the United States.Using trauma theory as a tool,this paper explores the complex relationship between the deep-seated causes of the tragedy of the two female characters in the play and the historical context through the traumatic events experienced by the characters in the play and the different attitudes they show in the face of trauma.Through this,the vulnerability and identity crisis of individuals during the period of social change and cultural breaks are analyzed.The purpose of the paper is to arouse human beings’thoughts on how to cope with cultural integration and foreign cultural invasion,and how to obtain salvation when they are amid an identity crisis.

Consumer Culture in The Great Gatsby

F.Scott Fitzgerald’s The Great Gatsby successfully portrays the“Jazz Age”.In the early 20th century,the United States transitioned from a production-based society to a consumer-based society,leading to an era of unparalleled prosperity.However,in the context of consumer culture,individuals often transition from being masters of their possessions to becoming enslaved by them.As a result,their behaviours and values become increasingly associated with extravagance and consumption.This paper uses Baudrillard’s theory of consumer society and textual analysis to explore the impact of consumer culture on human beings through the symbolization of men and objectification of women in The Great Gatsby.The meanings embedded in the novel are revealed from a different perspective.

Coupling coordination analysis of Mazu culture,socio-economy and ecological environment of Meizhou Island

Taking the pilgrimage,tourism and cultural island of Meizhou Island as an example,the evaluation index system of the coupling and coordinated development of“Mazu culture,socio-economy,eco-environment”(MSE)compound system was constructed.The index weights were determined by AHP-entropy method,and the coupling degree,coordinated degree,comprehensive evaluation index and grey correlation degree of MSE system of Mazu Island from 2012 to 2022 were measure.The results showed that:(1)the comprehensive evaluation indexes of the three subsystems was on the rise in general,but the evaluation index of the ecological subsystems increased relatively slowly.(2)The coupling degree was only in the running-in stage in 2012,and the other years were in the coordinated coupling stage.(3)The coupling coordination degree increased from 0.35 in 2012 to 0.82 in 2022,the coupling coordination level was changed from mild imbalance to good coordination.(4)Through the comparison of grey correlation degree,the 24 indexes in the evaluation index system had great influence on the coupling coordination degree of MSE system.The coupling coordination degree was closely related to the development of socio-economy and the spread of Mazu culture.With the rapid development of tourism brought about by the spread of Mazu culture,the pressure on the ecological environment will be increasing.Compared with the rapid growth of tourism and economy,it is equally important to strengthen environmental protection and pay attention to the quality of ecological environment development.

The Application of the Domestication and Foreignization Translation Strategies in the Context of the Dissemination of Chinese Culture:A Case Study of the English Translation of Long

In January 2024,the Chinese Year of the Loong,how to translate the Chinese character Long went viral on the Internet.Taking the English translation of Long as an example,this study tends to make a tentative analysis of the application of translation strategies in the macro context of the dissemination of Chinese culture.Moreover,it probes into the phenomenon of Chinese cultural aphasia by analyzing the domestication and foreignization of Long in three aspects.The three attitudes provided ought to be addressed in the Chinese-English translations.This study emphasizes enhancing translators’cultural awareness and their cultural confidence in the process of translating the source language to the target language.

Research on the Enhancement of Chongqing Cyberpunk Cultural Tourism Experience under the Background of Culture and Tourism Integration

As a new trend in tourism and cultural development,the integration of culture and tourism is capturing the zeitgeist with its unique charm.In this context,various regions have explored distinctive cultural tourism resources and created unique tourism brands through in-depth exploration and innovation of local culture.Chongqing,with its unique geomorphological features and distinctive architectural style,retains the deep flavor of traditional Chinese culture while cleverly integrating the futuristic sense of modern technology.This combination aligns perfectly with today’s popular Chinese cyberpunk style,making Chongqing an ideal place for the development of cyberpunk cultural tourism.This article investigates the history of cyberpunk culture and its current development status in Chongqing through a literature review.It then discusses the feasibility of developing cyberpunk cultural tourism in terms of social and cultural development needs and market demand.Based on a survey of cyberpunk tourists’experiences,the article deeply analyzes the existing problems of cyberpunk cultural tourism in Chongqing and proposes corresponding experience enhancement strategies,aiming to provide references for the development of cyberpunk cultural tourism in Chongqing Municipality.

The Cult of Mazu in Hainan,China and Among Vietnamese Hainanese Diaspora:A Comparative Study of Mazu Culture

The cult of Mazu is a common folk belief in China and Vietnam.Originating from the Song and Yuan Dynasties,particularly during the Ming and Qing Dynasties,the cult of Mazu spread to Vietnam through maritime trade and migration from coastal regions of China.After centuries of integration and development,it evolved into its own cultural system of Mazu worship,becoming an important component of Vietnamese folk culture.Hainan,China,as a crucial supply station on the Maritime Silk Road,has close connections with the transmission of Mazu culture to Vietnam.This paper adopts field investigations and literature research methods to explore the Mazu worship and culture in Hainan,China,and the Qiongfu Assembly Halls in Vietnam.

Chinese Culture,Classical Philosophy,and the Cultivation of Life

This paper explores the intricate relationship between Chinese culture and the nation’s rise,emphasizing the indispensable role of cultural education in fostering a robust national spirit and civilization.Drawing from classical Chinese texts and contemporary philosophical insights,the study examines how cultural virtues and educational practices can lead to societal harmony,national rejuvenation,and global contributions.The analysis highlights the enduring relevance of Confucian principles and their potential to address modern challenges,advocating for a renewed focus on cultural education to achieve a prosperous and peaceful world.Additionally,it underscores the necessity of integrating traditional values with modern educational practices to create a holistic approach that fosters ethical leadership and global cooperation.