Ionic liquid-based transparent membrane-coupled human lung epithelium-on-a-chip demonstrating PM0.5 pollution effect under breathing mechanostress

The plausibility of human exposure to particulate matter(PM)has witnessed an increase within the last several years.PM of different sizes has been discovered in the atmosphere given the role of dust transport in weather and climate composition.As a regulator,the lung epithelium orchestrates the innate response to local damage.Herein,we developed a lung epithelium-ona-chip platform consisting of easily moldable polydimethylsiloxane layers along with a thin,flexible,and transparent ionic liquid-based poly(hydroxyethyl)methacrylate gel membrane.The epithelium was formed through the culture of human lung epithelial cells(Calu-3)on this membrane.The mechanical stress at the air–liquid interface during inhalation/exhalation was recapitulated using an Arduino-based servo motor system,which applied a uniaxial tensile strength from the two sides of the chip with 10%strain and a frequency of 0.2 Hz.Subsequently,the administration of silica nanoparticles(PM0.5)with an average size of 463 nm to the on-chip platform under static,dynamic,and dynamic+mechanical stress(DMS)conditions demonstrated the effect of environmental pollutants on lung epithelium.The viability and release of lactate dehydrogenase were determined along with proinflammatory response through the quantification of tumor necrosis factor-α,which indicated alterations in the epithelium.

Amethod for sharing dynamic geometry information in studies on liquid-based detectors

Liquid-based detectors are widely used in particle and nuclear physics experiments.Because fixed method is used to construct the geometry in detector simulations such as Geant4,it is usually difficult to describe the non-uniformity of the liquid in a detector.We propose a method based on geometry description markup language and a tessellated detector description to share the detector geometry information between computational fluid dynamics simulation software and detector simulation software.This method makes it possible to study the impact of a liquid flow and non-uniformity on the key performance of a liquid-based detector,such as the event vertex reconstruction resolution.This will also be helpful in the detector design and performance optimization.

Comparison of smear cytology with liquid-based cytology in pancreatic lesions: A systematic review and meta-analysis

BACKGROUND Endoscopic ultrasonography-guided fine-needle aspiration(EUS-FNA)is a safe and accurate technique to confirm the diagnosis of pancreatic cancers.Recently,numerous studies comparing the diagnostic efficacy of smear cytology(SC)and liquid-based cytology(LBC)for pancreatic lesions yielded mixed results.AIM To compare and identify the better cytology method for EUS-FNA in pancreatic lesions.METHODS A comprehensive search of PubMed,Embase,and Cochrane was undertaken through July 18,2020.The primary endpoint was diagnostic accuracy(sensitivity and specificity).Secondary outcomes included sample adequacy and post procedure complications.In addition,factors affecting diagnostic efficacy were discussed.RESULTS Data on a total of 1121 comparisons from 10 studies met the inclusion criteria.Pooled rates of sensitivity for SC and LBC were 78%(67%-87%)vs 75%(67%-81%),respectively.In any case,both SC and LBC exhibited a high specificity close to 100%.Inadequate samples more often appeared in LBC compared with SC.However,the LBC samples exhibited a better visual field than SC.Very few post procedure complications were observed.CONCLUSION Our data suggested that for EUS-FNA in pancreatic lesions(particularly solid lesions),SC with Rapid On-Site Evaluation represents a superior diagnostic technique.If Rapid On-Site Evaluation is unavailable,LBC may replace smears.The diagnostic accuracy of LBC depends on different LBC techniques.

Diagnostic value of liquid-based cytology and smear cytology in pancreatic endoscopic ultrasound-guided fine needle aspiration: A meta-analysis

BACKGROUND Smear cytology(SC)using endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)is the established and traditional choice for diagnosing pancreatic lesions.Liquid-based cytology(LBC)is a novel alternative cytological method,however,the comparative diagnostic efficacy of LBC remains inconclusive.AIM To examine the diagnostic efficacy of LBC and SC for pancreatic specimens obtained through EUS-FNA via a systematic review and meta-analysis.METHODS A systematic literature search was performed using PubMed,EMBASE,the Cochrane Library,and Web of Science.The numbers of true positives,false positives,true negatives,and false negatives for each cytological test(LBC and CS)were extracted from the included studies.The pooled sensitivity and specificity and the area under the summary receiver operating characteristic curve(AUC)were calculated,and the AUC was compared by Tukey's multiple comparisons test.The quality of the included studies was assessed using the Quality Assessment of Diagnostic Accuracy Studies II tool.RESULTS A total of 1656 patients in eight studies were included.The pooled sensitivity and specificity and the AUC for LBC were 0.76(95%CI:0.72-0.79),1.00(95%CI:0.98-1.00),and 0.9174,respectively,for diagnosing pancreatic lesions.The pooled estimates for SC were as follows:Sensitivity,0.68(95%CI:0.64-0.71);specificity,0.99(95%CI:0.96-100.00);and AUC,0.9714.Similarly,the corresponding values for LBC combined with SC were 0.87(95%CI:0.84-0.90),0.99(95%CI:0.96-1.00),and 0.9894.Tukey’s multiple comparisons test was used to compare the sensitivities and AUCs of the three diagnostic methods;statistically significant differences were found between the three methods,and LBC combined with SC was superior to both LBC(P<0.05)and SC(P<0.05).The pooled sensitivity and AUC did not change significantly in the sensitivity analysis.CONCLUSION LBC may be sensitive than SC in the cytological diagnosis of pancreatic lesions,however,the superior diagnostic performance of their combination emphasizes their integrated usage in the clinical evaluation of pancreatic lesions.

Comparative Study of Cell Findings by Conventional Smear and Liquid-Based Cytology for Oral Exfoliative Cytology

Background: Liquid-based cytology (LBC) is a method of manufacturing cyto-diagnostic specimens. Improved accuracy is expected from standardizing specimen production and use of this method is rapidly spreading in oral cytology. On the other hand, LBC reportedly requires training to show peculiar cell findings compared to those of conventional smear cytology (CVC). Few studies have compared detailed cell findings for oral CVC and LBC. Objectives: The aim of this study was to compare cytological findings between CVC and LBC using cytomorphological image analysis. Materials and Methods: Cytological specimens were collected from 20 patients (negative for squamous neoplasia in 10, dysplasia in 5, squamous cell carcinoma in 5) and 5 controls of the tongue between January 2017 and December 2018. Two different preparation techniques were investigated cytomorphologically for CVC and LBC (BD CytorichTM). Results: LBC showed significantly higher cell numbers than CVC for all lesions. LBC-to-CVC ratio ranged from 9.52 (hyperkeratosis) to 1.87 (deep cells in oral squamous cell carcinoma (OSCC)). Nuclear area of normal, hyperkeratosis, and inflammation were significantly higher in LBC than those of CVC. Hyperchromasia was significantly more frequent with CVC than with LBC for hyperkeratosis, inflammation, dysplasia and OSCC. There was no significant difference in circularity between CVC and LBC among all lesions. Conclusion: Only one cytomorphological disadvantage was seen with LBC, in the form of decreased hyperchromasia. Further clarification of the advantages and disadvantages of LBC is needed, including management of precision and screening practices.

基于Slide的研山铁矿高陡边坡稳定性分析

露天矿山边坡稳定性对采场安全生产至关重要。为探究边坡施工过程中的安全隐患,以研山铁矿高陡边坡为研究对象,考虑爆破震动及地震作用荷载,运用Slide二维边坡数值模拟软件对典型剖面进行稳定性计算。结果表明:在正常工况条件、爆破震动及地震作用下,边坡整体安全系数符合要求,整体处于稳定状态。但在爆破震动及地震作用下,边坡局部安全系数介于临界值,可能存在局部滑坡风险。为保证采场生产安全,提出了相应的边坡管理预防措施,为相关实践工作提供一定参考。

Vaginal Intraepithelial Neoplasia Detected with Cervical Liquid-Based Cytology: Old Concerns or New Facilities?

Background: The detection of vaginal intraepithelial neoplasia (VAIN) in cervical samples is not a common finding. Therefore, we aimed to report VAINs detected in liquid-based cytology (LBC) from women examined at Hospital das Clínicas of Faculty of Medicine, Sao Paulo State University. Materials and Methods: We evaluated LBC samples from women referred to gynecology examination for different reasons (previous abnormal PapTest, follow up of treated cervical lesion, ecc) and women examined for regular screening proposals, and compared with biopsy diagnoses, including the controversial diagnoses of vaginal intraepithelial neoplasia (VAIN). Results: From 1866 patients, 1423 (76.3%) cases were negative and 443 (23.7%) were positive for any cellular alteration. Age of patients ranged from 12 to 86 years. We detected 25 histologically confirmed VAIN (1.3% p = 0.0002 by Fisher’s exact test IC 95% 0.0090 - 0.0198) and 1.1% VAIN (p = 0.0031 by Fisher’s exact test IC 95% 0.0077 - 0.0179). Conclusion: The identification of VAIN in routine is feasible;the professionals involved with cytological examination should be aware of these lesions in Pap test samples.

Predicting impact forces on pipelines from deep-sea fluidized slides:A comprehensive review of key factors

Deep-sea pipelines play a pivotal role in seabed mineral resource development,global energy and resource supply provision,network communication,and environmental protection.However,the placement of these pipelines on the seabed surface exposes them to potential risks arising from the complex deep-sea hydrodynamic and geological environment,particularly submarine slides.Historical incidents have highlighted the substantial damage to pipelines due to slides.Specifically,deep-sea fluidized slides(in a debris/mud flow or turbidity current physical state),characterized by high speed,pose a significant threat.Accurately assessing the impact forces exerted on pipelines by fluidized submarine slides is crucial for ensuring pipeline safety.This study aimed to provide a comprehensive overview of recent advancements in understanding pipeline impact forces caused by fluidized deep-sea slides,thereby identifying key factors and corresponding mechanisms that influence pipeline impact forces.These factors include the velocity,density,and shear behavior of deep-sea fluidized slides,as well as the geometry,stiffness,self-weight,and mechanical model of pipelines.Additionally,the interface contact conditions and spatial relations were examined within the context of deep-sea slides and their interactions with pipelines.Building upon a thorough review of these achievements,future directions were proposed for assessing and characterizing the key factors affecting slide impact loading on pipelines.A comprehensive understanding of these results is essential for the sustainable development of deep-sea pipeline projects associated with seabed resource development and the implementation of disaster prevention measures.

CrossLinkNet: An Explainable and Trustworthy AI Framework for Whole-Slide Images Segmentation

In the intelligent medical diagnosis area,Artificial Intelligence(AI)’s trustworthiness,reliability,and interpretability are critical,especially in cancer diagnosis.Traditional neural networks,while excellent at processing natural images,often lack interpretability and adaptability when processing high-resolution digital pathological images.This limitation is particularly evident in pathological diagnosis,which is the gold standard of cancer diagnosis and relies on a pathologist’s careful examination and analysis of digital pathological slides to identify the features and progression of the disease.Therefore,the integration of interpretable AI into smart medical diagnosis is not only an inevitable technological trend but also a key to improving diagnostic accuracy and reliability.In this paper,we introduce an innovative Multi-Scale Multi-Branch Feature Encoder(MSBE)and present the design of the CrossLinkNet Framework.The MSBE enhances the network’s capability for feature extraction by allowing the adjustment of hyperparameters to configure the number of branches and modules.The CrossLinkNet Framework,serving as a versatile image segmentation network architecture,employs cross-layer encoder-decoder connections for multi-level feature fusion,thereby enhancing feature integration and segmentation accuracy.Comprehensive quantitative and qualitative experiments on two datasets demonstrate that CrossLinkNet,equipped with the MSBE encoder,not only achieves accurate segmentation results but is also adaptable to various tumor segmentation tasks and scenarios by replacing different feature encoders.Crucially,CrossLinkNet emphasizes the interpretability of the AI model,a crucial aspect for medical professionals,providing an in-depth understanding of the model’s decisions and thereby enhancing trust and reliability in AI-assisted diagnostics.

基于SLIDE软件的某矿山排土场稳定性分析

为研究某矿山排土场稳定性,使用SLIDE软件,采用简化Bishop法、Janbu修正法和Spencer法对该排土场自然工况、降雨工况和地震工况下的安全系数进行计算。结果表明,该排土场边坡在自然和降雨工况下处于稳定,在地震工况下处于基本稳定,由此为该排土场将来的维护和治理作业提供了参考价值。

基于Slide2的某铁矿尾矿库坝体稳定性分析

尾矿库是冶金矿山的重要附属设施,一旦失事将造成严重的环境污染和重大损失,尾矿库安全的核心在于尾矿坝是否稳定。通过采用Slide2软件,根据岩土工程勘察资料,建立山西某铁矿尾矿库坝体典型剖面的二维数值模型,对坝体三种运行工况下的渗流稳定性和坝坡稳定性进行分析,以确定尾矿库的安全性,并分析Slide2软件应用于尾矿坝稳定性分析中的可靠性和功能性。

基于Slide的Webdav服务器的设计与实现

描述了一个支持Webdav协议的文档管理系统，它以Apache的Jakarta项目组中的Slide模块为实现基础，任何支持Webdav协议的客户端都可以方便地使用该系统。

SLIDE在深基坑支护可靠性分析中的应用

基于可靠性分析理论,利用SLIDE软件,对某深基坑复合土钉支护进行了数值模拟,计算了安全系数和可靠性指标。结果表明:SLIDE是基坑可靠性分析的一种有效工具,可在考虑土层参数不确定性的条件下,得出基坑支护的可靠性指标。

支气管镜在儿童Slide气管成形术中的应用

目的 总结支气管镜在儿童Slide气管成形术中的作用。方法 回顾性分析2017—2020年湖南省人民医院收治的4例气管狭窄患儿的诊疗经过,总结支气管镜在Slide气管成形术术前评估、术中定位与测量及术后创面评估和治疗中的作用。结果 Slide气管成形术术前支气管镜评估显示,4例患儿中3例存在完全气管环,2例合并肺动脉吊带,2例存在多处狭窄。3例在该院完成Slide气管成形术,术中支气管镜下判断狭窄段中点及测量狭窄段长度,协助离断气管狭窄段,术后灌洗明确病原;1例外院Slide气管成形术术后9个月出现瘢痕牵拉,经支气管镜下介入治疗好转。2例术后第4天支气管镜下发现气管黏膜改变,调整治疗方案;2例术后1个月出现肉芽增生,经支气管镜下冷冻治疗好转。1例因吻合口坏死放弃治疗死亡,存活的3例随访6个月以上,预后可,但均存在气管支气管软化。结论 支气管镜可用于气管狭窄患儿Slide气管成形术的管理,有助于术后康复及随访。

基于Slide的某钨矿尾矿坝稳定性及概率分析

尾矿坝的稳定运行是钨矿山安全生产的重要保障。研究结合某钨矿尾矿库的工程地质特征,采用Slide软件建立坝体的概化模型,通过Bishop法计算尾矿坝在正常、洪水及特殊三种工况下的稳定性,结合瑞典条分法对坝体的抗滑稳定最小安全系数进行校核。同时运用概率分析法,对安全系数在服从正态分布及对数正态分布下开展可靠性指数的计算,并得出坝体的失效概率。综合分析坝体的稳定性和概率分析的结果,认为该尾矿坝在三种工况下能正常运行但存在洪水漫顶和地震液化的隐患,并针对隐患提出相应的整改建议。

数字音频广播Slide Show业务的研究与实现

该文重点介绍了数字音频广播数据业务中Slide Show业务的数据结构,然后详细分析说明了Slide Show业务数据的传输过程及其解码的具体方法和流程,并给出了基于计算机的DMBPlayer平台上实现与验证方案。

基于Slide程序对云南某矿排土场边坡稳定性与不确定性分析

露天矿排土场边坡的稳定性对露天矿山安全有序的生产与运行起着至关重要的作用。该文采用极限平衡法对排土场边坡进行了稳定性分析,通过排土场边坡的各种不确定影响因素及其相互关系,对排土场边坡进行了不确定性分析。基于Slide程序对云南某露天矿排土场进行了简要分析与模拟,其模拟结果与该排土场现状基本吻合,同时分析了该排土场的不确定性,为今后如何进行堆排提出了建议。

Slide在边坡稳定分析中的应用——以某水电站放空洞出水口边坡为例

在各种边坡稳定分析方法中,极限平衡分析法是应用最广泛的方法之一。Slide软件主要采用毕肖普、简布、Spencer等方法对边坡的稳定系数及失效概率进行二维分析,可用于解决圆弧、非圆弧及复合滑移面等多种滑移面的土质及岩质边坡稳定问题。利用该软件对某水电站放空洞出水口边坡的稳定性进行分析,为边坡下步开挖与支护提供地质依据。

Slide软件在边坡稳定性计算中的应用

介绍了一款新的边坡稳定性计算的专业软件Slide,并结合某桥梁的工程实例,运用Slide软件进行建模和计算分析。

基于数控系统的Slide Show模块的开发与设计

针对数控系统提出了一种以显示图片的方式介绍系统功能的Slide Show模块设计方案。作者分析了图片设计的要求与标准,讨论了Slide Show模块的实现方法,并且提供了程序执行的流程图。文章分析了Slide Show模块的性能,对目前还存在的问题提出改进的设想。实践证明：使用Slide Show模块提高了用户对数控系统的使用效率。