对一养殖场所发生的大菱鲆(S cophtha lm usm ax im us L.)病害进行了发病情况、临床表现、病理变化及病原等方面的检验,结果表明为细菌性败血感染症。通过对15尾病(死)大菱鲆病变组织中细菌检查、细菌分离与鉴定,以及人工感染试验的致...对一养殖场所发生的大菱鲆(S cophtha lm usm ax im us L.)病害进行了发病情况、临床表现、病理变化及病原等方面的检验,结果表明为细菌性败血感染症。通过对15尾病(死)大菱鲆病变组织中细菌检查、细菌分离与鉴定,以及人工感染试验的致病作用等,表明所检出的细菌为利斯顿氏菌属(L istonella M acD one ll and Co lw e ll 1986)的鳗利斯顿氏菌[L.angu illarum(B ergem an 1909)M acD one ll and Co lw e ll 1986],系该病例的病原菌。展开更多
Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs...Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs of the diseased tongue sole included several petechiae and ecchymoses on the body and fin necrosis and hemorrhagic lesion at the base of the fin. Bacteria were isolated from kidney, liver and hemorrhagic lesions of the diseased tongue sole. Among 14 strains, SJ060621 was proved to be highly virulent to juvenile tongue sole with LD50 value of 〈1.0×10^5 colony forming units （CFU）mL^-1, while the remaining 13 were avirulent. Among the 16 antibiotics tested, SJ060621 was sensitive to gentamicin and nitrofurantoin. It was identified as Listonella anguillantm with conventional plate and tube tests in combination with API 20E analysis. 16S rRNA gene and partial HSP60 gene sequenceing analysis revealed that the strain was highly homologous with L. anguillarum. Examination of the infected musculature by electron microscopy indicated numerous bacteria and lots of macrophages containing phagocytosed bacteria. Histopathological investigations revealed severe necrotic degenerative changes in the infected organs. Indirect immunofluorescence assay （IFA） was employed to detect the location of occurrence of bacteria, and bacteria were found in aggregations in the inflammatory areas in musculature.展开更多
Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium （Cd） on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The ...Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium （Cd） on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase （SOD）, glutathione peroxidase （GPx）, acid phosphatase （ACP）, Na＋, K＋-ATPase in gills and hepatopancreas, and phenoloxidase-like （POL） enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 （IrtSP9~））, metallothionein （MT）, and bactericidal/permeability increasing （BPI） protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na＋, K＋-ATPase activities in both tissues, and MT expression was increased in Cd- exposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters＇ immune responses and energy metabolism following vibrio infection.展开更多
对从大菱鲆(Turbot,Scophthalmus maximus L.)细菌性败血感染症的病(死)鱼中分离到并经鉴定的病原鳗利斯顿氏菌(Listonella anguillarum)进行了对常用抗菌类药物及弧菌抑制剂O/129的敏感性测定。结果表明,供试的20株鳗利斯顿氏菌均对所...对从大菱鲆(Turbot,Scophthalmus maximus L.)细菌性败血感染症的病(死)鱼中分离到并经鉴定的病原鳗利斯顿氏菌(Listonella anguillarum)进行了对常用抗菌类药物及弧菌抑制剂O/129的敏感性测定。结果表明,供试的20株鳗利斯顿氏菌均对所测定的37种抗菌类药物中的头孢噻肟等18种药物高度敏感、对青霉素G等6种药物耐药、对头孢唑啉等10种药物耐药性菌株间有差异;2株(S010610-3和S010610-4)对弧菌抑制剂O/129具抗性,余18株均表现敏感。展开更多
Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic infor...Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic information of pathogenic bacteria was studied, including morphology, physiological and biochemical characteristics, and the mol% G+C of the DNA. In addition, representative strains (S010623-1, LH031120-1) were selected for molecular identification by partial 16S rRNA gene sequencing. The results show that the isolates (LH031120-1 to LH031120-6, HT040308-1 to HT040308-6, HT040620-1 to HT040620-6) from three farms were identified as Edwardsiella tarda. The isolates (S010610-1 to S010610-10, S010623-1 to S010623-20) from one farm were identified as Listonella anguillarum. We conducted studies on the pathogenicity of isolates by artificial infection, and revealed all infected groups in morbidity and mortality. The septicaemia infected turbot showed a syndrome similar to that of the naturally infected fish. Antibiotic sensitivity showed that of 37 antimicrobial agents, E. tarda was sensitive to 27 agents, and L. anguillarum was sensitive to 21 agents.展开更多
文摘对一养殖场所发生的大菱鲆(S cophtha lm usm ax im us L.)病害进行了发病情况、临床表现、病理变化及病原等方面的检验,结果表明为细菌性败血感染症。通过对15尾病(死)大菱鲆病变组织中细菌检查、细菌分离与鉴定,以及人工感染试验的致病作用等,表明所检出的细菌为利斯顿氏菌属(L istonella M acD one ll and Co lw e ll 1986)的鳗利斯顿氏菌[L.angu illarum(B ergem an 1909)M acD one ll and Co lw e ll 1986],系该病例的病原菌。
基金This study was supported by National High Technology Development Program of China(863,Grant 2006AA100306)the Science Foundation of Shandong Province(032070104).
文摘Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs of the diseased tongue sole included several petechiae and ecchymoses on the body and fin necrosis and hemorrhagic lesion at the base of the fin. Bacteria were isolated from kidney, liver and hemorrhagic lesions of the diseased tongue sole. Among 14 strains, SJ060621 was proved to be highly virulent to juvenile tongue sole with LD50 value of 〈1.0×10^5 colony forming units （CFU）mL^-1, while the remaining 13 were avirulent. Among the 16 antibiotics tested, SJ060621 was sensitive to gentamicin and nitrofurantoin. It was identified as Listonella anguillantm with conventional plate and tube tests in combination with API 20E analysis. 16S rRNA gene and partial HSP60 gene sequenceing analysis revealed that the strain was highly homologous with L. anguillarum. Examination of the infected musculature by electron microscopy indicated numerous bacteria and lots of macrophages containing phagocytosed bacteria. Histopathological investigations revealed severe necrotic degenerative changes in the infected organs. Indirect immunofluorescence assay （IFA） was employed to detect the location of occurrence of bacteria, and bacteria were found in aggregations in the inflammatory areas in musculature.
基金Supported by the 100 Talents Program of Chinese Academy of Sciencesthe Development Plan of Science and Technology in Shandong Province(No.2012GGA06032)the Key Deployment Program of Chinese Academy of Sciences(No.KZZD-EW-14-03)
文摘Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium （Cd） on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase （SOD）, glutathione peroxidase （GPx）, acid phosphatase （ACP）, Na＋, K＋-ATPase in gills and hepatopancreas, and phenoloxidase-like （POL） enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 （IrtSP9~））, metallothionein （MT）, and bactericidal/permeability increasing （BPI） protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na＋, K＋-ATPase activities in both tissues, and MT expression was increased in Cd- exposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters＇ immune responses and energy metabolism following vibrio infection.
文摘对从大菱鲆(Turbot,Scophthalmus maximus L.)细菌性败血感染症的病(死)鱼中分离到并经鉴定的病原鳗利斯顿氏菌(Listonella anguillarum)进行了对常用抗菌类药物及弧菌抑制剂O/129的敏感性测定。结果表明,供试的20株鳗利斯顿氏菌均对所测定的37种抗菌类药物中的头孢噻肟等18种药物高度敏感、对青霉素G等6种药物耐药、对头孢唑啉等10种药物耐药性菌株间有差异;2株(S010610-3和S010610-4)对弧菌抑制剂O/129具抗性,余18株均表现敏感。
基金Major State Basic Research Development Program (No. G1999012002)Science Technology Department Project of Hebei Province (No. 302431)
文摘Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic information of pathogenic bacteria was studied, including morphology, physiological and biochemical characteristics, and the mol% G+C of the DNA. In addition, representative strains (S010623-1, LH031120-1) were selected for molecular identification by partial 16S rRNA gene sequencing. The results show that the isolates (LH031120-1 to LH031120-6, HT040308-1 to HT040308-6, HT040620-1 to HT040620-6) from three farms were identified as Edwardsiella tarda. The isolates (S010610-1 to S010610-10, S010623-1 to S010623-20) from one farm were identified as Listonella anguillarum. We conducted studies on the pathogenicity of isolates by artificial infection, and revealed all infected groups in morbidity and mortality. The septicaemia infected turbot showed a syndrome similar to that of the naturally infected fish. Antibiotic sensitivity showed that of 37 antimicrobial agents, E. tarda was sensitive to 27 agents, and L. anguillarum was sensitive to 21 agents.
