Alexandrium pacificum(A.pacificum)is a typical paralytic shellfish poisonous dinoflagellate.Harmful algal blooms(HABs)caused by this species can bring serious environmental problems and economic losses to the aquacult...Alexandrium pacificum(A.pacificum)is a typical paralytic shellfish poisonous dinoflagellate.Harmful algal blooms(HABs)caused by this species can bring serious environmental problems and economic losses to the aquaculture industry.In this study,transcriptome sequencing and analyses were performed on the neural tissue of Litopenaeus vannamei(L.vannamei)after acute exposure to A.pacificum disrupted solution for 72 h,and differentially expressed genes(DEGs)were identified.The results showed that,compared with the control samples,300 DEGs were identified in the experimental group,of which 194 were up-regulated,and 106 down-regulated.The gene ontology(GO)functional enrichment analysis showed that DEGs were significantly enriched in the cortical cytoskeleton organization,troponin complex,amylo-alpha-1,6-glucosidase and thymidine phosphorylase.Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis found that DEGs were mainly enriched in the oxidative phosphorylation process,intercellular tight junctions and mitophagy.The results showed that the proteoglycans,signaling pathways,and various metabolic processes that regulate cell proliferation,differentiation,and apoptosis all played an essential role in the response of L.vannamei to A.pacificum toxins.展开更多
Quality deterioration induced by microbial community in Litopenaeus vannamei during superchilling storage were demonstrated in this study.In this study,6 microorganisms were identified as the biomarkers by 16S rDNA se...Quality deterioration induced by microbial community in Litopenaeus vannamei during superchilling storage were demonstrated in this study.In this study,6 microorganisms were identified as the biomarkers by 16S rDNA sequencing and 71 differential metabolites were identified by untargeted metabolomics.The correlation analysis between microorganisms and differential metabolites revealed that Oceanicaulis,Aliihoeflea,Prauserella,Chelativorans and Pseudoalteromonas had the potential to be the important microorganisms of deterioration of superchilled shrimp.Moreover,the spearman correlation analysis revealed that lightness value had highly significant correlation with Shewanella(P<0.01),and thiobarbituric acid content,total viable count value,total sulfhydryl content,trichloroacetic acid-soluble peptide and Ca2+-ATPase activity showed significantly correlation with Oceanicaulis,Prauserella,Chelativorans,Aliihoefl ea and Pseudoalteromonas(P<0.05).This study offers a better understanding for mechanisms behind quality degradation induced by microorganisms,which may help the quality maintaining of Pacific white shrimp during superchilling storage.展开更多
利用7个凡纳滨对虾(Litopenaeus vannamei)引进群体,通过双列杂交和巢式交配设计构建家系,建立育种基础群体,估计体重、存活性状的遗传参数和基因型与环境互作效应(genotype by environment interactions,G×E)。结果表明,凡纳滨对...利用7个凡纳滨对虾(Litopenaeus vannamei)引进群体,通过双列杂交和巢式交配设计构建家系,建立育种基础群体,估计体重、存活性状的遗传参数和基因型与环境互作效应(genotype by environment interactions,G×E)。结果表明,凡纳滨对虾基础群体体重和存活性状的遗传力估计值范围分别在(0.19±0.09)—(0.43±0.09),(0.27±0.04)—(0.45±0.06),均属于中高遗传力水平,并且统计检验显著(P<0.05)。Z-score检验表明,体重和存活性状遗传力估计值在河北黄骅(HBHH)和青岛鳌山(QBAS)两个测试场间差异均不显著。体重和存活性状的表型和遗传相关系数分别为0.007和0.008,表现为低度线性负相关。对于体重和存活性状,HBHH和QBAS场间的遗传相关分别为0.83±0.04和0.40±0.11。体重性状的G×E效应不显著(K<0.5),但存活性状存在显著的G×E效应(K>0.5)。上述结果显示,凡纳滨对虾基础群体体重和存活性状的遗传变异丰富,多代选择可获得较大的遗传进展。展开更多
基金supported by the Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province(No.K22226)the National Natural Science Foundation of China(No.32102796)+3 种基金the Natural Science Foundation of Guangdong Province(No.2020A1515110086)the Program for Scientific Research Start-up Funds of Guangdong Ocean University(Nos.060302022102,060302022201)the Program of Shrimp Aquaculture Talent Development(No.B22424)the Undergraduate Innovation Team of Guangdong Ocean University(No.CXTD2023002).
文摘Alexandrium pacificum(A.pacificum)is a typical paralytic shellfish poisonous dinoflagellate.Harmful algal blooms(HABs)caused by this species can bring serious environmental problems and economic losses to the aquaculture industry.In this study,transcriptome sequencing and analyses were performed on the neural tissue of Litopenaeus vannamei(L.vannamei)after acute exposure to A.pacificum disrupted solution for 72 h,and differentially expressed genes(DEGs)were identified.The results showed that,compared with the control samples,300 DEGs were identified in the experimental group,of which 194 were up-regulated,and 106 down-regulated.The gene ontology(GO)functional enrichment analysis showed that DEGs were significantly enriched in the cortical cytoskeleton organization,troponin complex,amylo-alpha-1,6-glucosidase and thymidine phosphorylase.Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis found that DEGs were mainly enriched in the oxidative phosphorylation process,intercellular tight junctions and mitophagy.The results showed that the proteoglycans,signaling pathways,and various metabolic processes that regulate cell proliferation,differentiation,and apoptosis all played an essential role in the response of L.vannamei to A.pacificum toxins.
基金supported by the Guangdong Basic and Applied Basic Research Foundation(2020A1515110360)Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(320LH037)+3 种基金Hainan Provincial Natural Sciences Foundation of China(322QN434)Guangzhou Basic and Applied Basic Research Foundation(202102021062)Research and Development Projects in Key Areas of Guangdong Province,China(2021B0202060002)Postgraduate Education Innovation Project of Guangdong Ocean University(202242).
文摘Quality deterioration induced by microbial community in Litopenaeus vannamei during superchilling storage were demonstrated in this study.In this study,6 microorganisms were identified as the biomarkers by 16S rDNA sequencing and 71 differential metabolites were identified by untargeted metabolomics.The correlation analysis between microorganisms and differential metabolites revealed that Oceanicaulis,Aliihoeflea,Prauserella,Chelativorans and Pseudoalteromonas had the potential to be the important microorganisms of deterioration of superchilled shrimp.Moreover,the spearman correlation analysis revealed that lightness value had highly significant correlation with Shewanella(P<0.01),and thiobarbituric acid content,total viable count value,total sulfhydryl content,trichloroacetic acid-soluble peptide and Ca2+-ATPase activity showed significantly correlation with Oceanicaulis,Prauserella,Chelativorans,Aliihoefl ea and Pseudoalteromonas(P<0.05).This study offers a better understanding for mechanisms behind quality degradation induced by microorganisms,which may help the quality maintaining of Pacific white shrimp during superchilling storage.
文摘利用7个凡纳滨对虾(Litopenaeus vannamei)引进群体,通过双列杂交和巢式交配设计构建家系,建立育种基础群体,估计体重、存活性状的遗传参数和基因型与环境互作效应(genotype by environment interactions,G×E)。结果表明,凡纳滨对虾基础群体体重和存活性状的遗传力估计值范围分别在(0.19±0.09)—(0.43±0.09),(0.27±0.04)—(0.45±0.06),均属于中高遗传力水平,并且统计检验显著(P<0.05)。Z-score检验表明,体重和存活性状遗传力估计值在河北黄骅(HBHH)和青岛鳌山(QBAS)两个测试场间差异均不显著。体重和存活性状的表型和遗传相关系数分别为0.007和0.008,表现为低度线性负相关。对于体重和存活性状,HBHH和QBAS场间的遗传相关分别为0.83±0.04和0.40±0.11。体重性状的G×E效应不显著(K<0.5),但存活性状存在显著的G×E效应(K>0.5)。上述结果显示,凡纳滨对虾基础群体体重和存活性状的遗传变异丰富,多代选择可获得较大的遗传进展。
