The tree of Litsea cubeba is widely spread in China, Indonesia and other part of Southeast Asia. The essential oil of Litsea cubeba (EOLC) is obtained by steam distillation from the pepper-like fruits tree Litsea cube...The tree of Litsea cubeba is widely spread in China, Indonesia and other part of Southeast Asia. The essential oil of Litsea cubeba (EOLC) is obtained by steam distillation from the pepper-like fruits tree Litsea cubeba. The EOLC consists of about 29 active compounds. Among them, citral is the main component;the content of citral is nearly 80% of the EOLC. Due to the special function group, citral is easy to react with many chemicals. Thus, EOLC is usually applied as starting material to carry out aldol condensation, reduction, and six-member ring forming reaction. The EOLC is extensively employed to synthesis of geranal nitriles, pseudonoe, ionone, methyl ionone, Vitamin E and Vitamin A. These products are broadly applied in the fields of fragrance, perfume, medicine and so on. This paper presents comprehensive utilization of EOLC as raw materials to synthesize many active chemicals.展开更多
Anticancer activity in vitro ofLitsea cubeba fruit extracts was investigated, focusing on the fruit residue from essential oil extraction. The methanol extract was fractionated by an Amberlite XAD-7 column. Cell viabi...Anticancer activity in vitro ofLitsea cubeba fruit extracts was investigated, focusing on the fruit residue from essential oil extraction. The methanol extract was fractionated by an Amberlite XAD-7 column. Cell viability, cell proliferation and cell death were determined using conversion of WST-8, BrdU incorporation and measurement of released LDH, respectively. Activation of caspase-3/-7 was detected using Z-DEVD-R substrate and morphological characteristics of apoptotic cells were revealed by DAPI staining. It was found that 80-100% methanol fractions （RME-4B, -5A, -5B and -5C） were effective against HeLa cell viability and also promoted cell death. RME-SA and -5B were highly effective in suppressing DNA replication （IC50 4.89 and 3.26 g/mL at 48 h） and also in activation of caspase-3/-7 （9 and 17 times of untreated population at 12 h）. The presence of apoptotic bodies was clearly observed. The results of this study suggested that L. cubeba fruit residue has remarkable apoptosis induction potential for further use in cancer drug research and for waste management in the essential oil industry.展开更多
[ Objectlve] The aim of this study was to explore an explants induction method for L. cubeba. [ Method ] Different types of L. cubeba stem segments were collected at different time as explants materials for induction ...[ Objectlve] The aim of this study was to explore an explants induction method for L. cubeba. [ Method ] Different types of L. cubeba stem segments were collected at different time as explants materials for induction and culture. [ Result ] The results showed that sterilization with 0. 1% HgC12 for 10 min achieved better effects, with an explants contamination rate of dO. 0% and a mortality rate of 15.0% ; stem segments of the 2na -5th buds below the terminal bud were better explants materials for initial induction; the best sampling time was January, with an induction rate as high as 81.1%, while the contamination rate was only 11.7% ; modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA was the optimal medium for explants induction of L. cubeba. [ Conclusion] Semi-lignified stem segments of L cubeba collected in January, sterilized with 0.1% HgCI2 for 10 min and cultured in modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA a- chieved better induction effects on L. cubeba seedlings.展开更多
通过对山苍子幼嫩叶片、顶芽、花蕾3种组织的DNA提取效果分析和对影响酶切及选择性扩增效果的4个主要因素(酶切时间、Mg2+浓度、dNTPs浓度、引物浓度)的比较研究,建立了适合于山苍子AFLP分析的技术体系。结果表明,山苍子的顶芽是较好的...通过对山苍子幼嫩叶片、顶芽、花蕾3种组织的DNA提取效果分析和对影响酶切及选择性扩增效果的4个主要因素(酶切时间、Mg2+浓度、dNTPs浓度、引物浓度)的比较研究,建立了适合于山苍子AFLP分析的技术体系。结果表明,山苍子的顶芽是较好的DNA提取材料;山苍子基因组DNA经5 U EcoR I和5 U Mse I酶切1 h即可完全酶切;最佳的选择性扩增体系为20μL反应体系中含有1.0 U rTaq聚合酶、2.0μL 10×PCR缓冲液、1.8μL 25mmol.L-1MgCl2、1.4μL 2.5 mmol.L-1dNTP、100 ng.μL-1引物各1.0μL。使用该反应体系获得了清晰、稳定的DNA指纹图谱,并筛选出10对多态性较好的AFLP引物组合,为利用AFLP标记技术进一步开展山苍子种群遗传结构、遗传分化等研究奠定了基础。展开更多
为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、...为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好(R2>0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35%。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。展开更多
This paper was to determine the effects of dietary Litsea cubeba essential oil(LEO)supplementation on growth performance,immune function,antioxidant level,intestinal morphology and microbial composition in weaned pigl...This paper was to determine the effects of dietary Litsea cubeba essential oil(LEO)supplementation on growth performance,immune function,antioxidant level,intestinal morphology and microbial composition in weaned piglets.One hundred and ninety-two piglets(Duroc×[Large White×Landrace])with 6.85±0.22 kg mean body weight weaned at 21 d of age were randomly assigned to 4 treatment groups with 8 replicates and were fed with a basal diet(CON)or CON diet containing 100(LLEO),200(MLEO)and 400(HLEO)mg/kg LEO.The results revealed that HLEO supplementation(P<0.05)increased the average daily gain on d 28 compared with CON.MLEO and HLEO supplementation decreased(P<0.05)feed conversion ratio.LEO-containing diets had a lower(P<0.05)diarrhea rate.Supple-mentation with HLEO increased(P<0.05)total antioxidant capacity(T-AOC)both in the serum and liver.Meanwhile,the supplementation of MLEO and HLEO resulted in higher(P<0.05)glutathione peroxidase(GPx)activities both in serum and liver.Supplementation of HLEO increased(P<0.05)serum immu-noglobulin A,immunoglobulin G and interleukin-10,whereas supplementation with MLEO and HLEO decreased(P<0.05)tumor necrosis factor-α.Villus height in the duodenum or jejunum was increased(P<0.05)in the HLEO group,and the villus height to crypt depth ratio in the jejunum was also improved(P<0.05)in the MLEO group.The addition of LEO increased(P<0.05)the richness and diversity of the microbial community in the cecum,which mainly increased the relative abundance of Oscillospir-aceae_UCG-005,Faecalibacterium,Blautia and Coprococcus.Piglets supplemented with HLEO increased(P<0.05)the concentration of short chain fatty acids(SCFA),including acetic acid in the cecum and propionic acid in the colon.In conclusion,these findings indicated that LEO supplementation improved growth performance and intestinal health in weaned piglets.展开更多
文摘The tree of Litsea cubeba is widely spread in China, Indonesia and other part of Southeast Asia. The essential oil of Litsea cubeba (EOLC) is obtained by steam distillation from the pepper-like fruits tree Litsea cubeba. The EOLC consists of about 29 active compounds. Among them, citral is the main component;the content of citral is nearly 80% of the EOLC. Due to the special function group, citral is easy to react with many chemicals. Thus, EOLC is usually applied as starting material to carry out aldol condensation, reduction, and six-member ring forming reaction. The EOLC is extensively employed to synthesis of geranal nitriles, pseudonoe, ionone, methyl ionone, Vitamin E and Vitamin A. These products are broadly applied in the fields of fragrance, perfume, medicine and so on. This paper presents comprehensive utilization of EOLC as raw materials to synthesize many active chemicals.
文摘Anticancer activity in vitro ofLitsea cubeba fruit extracts was investigated, focusing on the fruit residue from essential oil extraction. The methanol extract was fractionated by an Amberlite XAD-7 column. Cell viability, cell proliferation and cell death were determined using conversion of WST-8, BrdU incorporation and measurement of released LDH, respectively. Activation of caspase-3/-7 was detected using Z-DEVD-R substrate and morphological characteristics of apoptotic cells were revealed by DAPI staining. It was found that 80-100% methanol fractions （RME-4B, -5A, -5B and -5C） were effective against HeLa cell viability and also promoted cell death. RME-SA and -5B were highly effective in suppressing DNA replication （IC50 4.89 and 3.26 g/mL at 48 h） and also in activation of caspase-3/-7 （9 and 17 times of untreated population at 12 h）. The presence of apoptotic bodies was clearly observed. The results of this study suggested that L. cubeba fruit residue has remarkable apoptosis induction potential for further use in cancer drug research and for waste management in the essential oil industry.
文摘[ Objectlve] The aim of this study was to explore an explants induction method for L. cubeba. [ Method ] Different types of L. cubeba stem segments were collected at different time as explants materials for induction and culture. [ Result ] The results showed that sterilization with 0. 1% HgC12 for 10 min achieved better effects, with an explants contamination rate of dO. 0% and a mortality rate of 15.0% ; stem segments of the 2na -5th buds below the terminal bud were better explants materials for initial induction; the best sampling time was January, with an induction rate as high as 81.1%, while the contamination rate was only 11.7% ; modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA was the optimal medium for explants induction of L. cubeba. [ Conclusion] Semi-lignified stem segments of L cubeba collected in January, sterilized with 0.1% HgCI2 for 10 min and cultured in modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA a- chieved better induction effects on L. cubeba seedlings.
文摘通过对山苍子幼嫩叶片、顶芽、花蕾3种组织的DNA提取效果分析和对影响酶切及选择性扩增效果的4个主要因素(酶切时间、Mg2+浓度、dNTPs浓度、引物浓度)的比较研究,建立了适合于山苍子AFLP分析的技术体系。结果表明,山苍子的顶芽是较好的DNA提取材料;山苍子基因组DNA经5 U EcoR I和5 U Mse I酶切1 h即可完全酶切;最佳的选择性扩增体系为20μL反应体系中含有1.0 U rTaq聚合酶、2.0μL 10×PCR缓冲液、1.8μL 25mmol.L-1MgCl2、1.4μL 2.5 mmol.L-1dNTP、100 ng.μL-1引物各1.0μL。使用该反应体系获得了清晰、稳定的DNA指纹图谱,并筛选出10对多态性较好的AFLP引物组合,为利用AFLP标记技术进一步开展山苍子种群遗传结构、遗传分化等研究奠定了基础。
文摘为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好(R2>0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35%。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。
基金National Key R&D Program of China(2021YFD1300403)the Youth Science Fund Project of the Natural Science Foundation of Hunan Province(2022JJ40165)Earmarked Fund for China Agricultural Research System(CARS-35)+1 种基金Hunan Provincial Key Research and Development Project(2020NK2031)National Natural Science Foundation of China(32130099).
文摘This paper was to determine the effects of dietary Litsea cubeba essential oil(LEO)supplementation on growth performance,immune function,antioxidant level,intestinal morphology and microbial composition in weaned piglets.One hundred and ninety-two piglets(Duroc×[Large White×Landrace])with 6.85±0.22 kg mean body weight weaned at 21 d of age were randomly assigned to 4 treatment groups with 8 replicates and were fed with a basal diet(CON)or CON diet containing 100(LLEO),200(MLEO)and 400(HLEO)mg/kg LEO.The results revealed that HLEO supplementation(P<0.05)increased the average daily gain on d 28 compared with CON.MLEO and HLEO supplementation decreased(P<0.05)feed conversion ratio.LEO-containing diets had a lower(P<0.05)diarrhea rate.Supple-mentation with HLEO increased(P<0.05)total antioxidant capacity(T-AOC)both in the serum and liver.Meanwhile,the supplementation of MLEO and HLEO resulted in higher(P<0.05)glutathione peroxidase(GPx)activities both in serum and liver.Supplementation of HLEO increased(P<0.05)serum immu-noglobulin A,immunoglobulin G and interleukin-10,whereas supplementation with MLEO and HLEO decreased(P<0.05)tumor necrosis factor-α.Villus height in the duodenum or jejunum was increased(P<0.05)in the HLEO group,and the villus height to crypt depth ratio in the jejunum was also improved(P<0.05)in the MLEO group.The addition of LEO increased(P<0.05)the richness and diversity of the microbial community in the cecum,which mainly increased the relative abundance of Oscillospir-aceae_UCG-005,Faecalibacterium,Blautia and Coprococcus.Piglets supplemented with HLEO increased(P<0.05)the concentration of short chain fatty acids(SCFA),including acetic acid in the cecum and propionic acid in the colon.In conclusion,these findings indicated that LEO supplementation improved growth performance and intestinal health in weaned piglets.
