Volatile oil from Litsea lancilimba Merr.: a review of its chemical structure and biological activity

Litsea lancilimba Merr.is a Cinnamomum plant of Lauraceae.Phytochemical investigations of the Litsea lancilimba Merr.have resulted in the isolation of Volatile oil,flavonoid glycosides,tannins,alkaloids,lignans,organic acids and so on.Studies have showed that volatile oil is the main chemical component.Recent trials have demonstrated the efficacy of Litsea lancilimba Merr.in strengthening the function of stomach.The fruits have also been demonstrated to possess anti-inflammatory and bacteriostatic activities.This review deals mainly with the chemical structure and biological activities of Litsea lancilimba Merr.oil.

Network pharmacology-based virtual screening of the active volatile oil ingredients of Litsea lancilimba Merr. and their potential targets for treating liver cancer

Liver cancer remains the dominant cause of cancer-related death, despite the advent of targeted therapies and immunotherapies. Therefore, there is an urgent need to explore novel chemotherapeutic agents and effective strategies against the cancer. There are a lot of monoterpenes and sesquiterpenoids in L. lancilimba. It has been reported that monoterpenes and sesquiterpenoids have certain anti-tumor effects. Literature investigation has also shown that Litsea lancilimba Merr. has antibacterial and antiviral effects. In this experiment, we used network pharmacology to screen the active compounds from L. lancilimba and their potential targets for treating liver cancer. Four active volatile oil ingredients and four potential targets have been identified. Our results provide the lead compound from L. lancilimba in the treatment of liver cancer.

Diverse sesquiterpenoids from Litsea lancilimba Merr.with potential neuroprotective effects against H_(2)O_(2)-induced SH-SY5Y cell injury

Five undescribed sesquiterpenoids(1–5),and nine known sesquiterpenoids(6–14)were obtained from the fruits of Litsea lancilimba Merr.by LC-MS/MS molecular networking strategies.Litsemene A(1)possessed a unique 8-member ring through unexpected cyclization of the methyl group on C-10 of guaiane.Their structures were elucidated by spectroscopic techniques including IR,UV,NMR,HR-ESI-MS,and their absolute configurations were assigned by ECD calculations.All isolated sesquiterpenoids were analyzed by bioinformatics and evaluated for their neuroprotective effects against H_(2)O_(2)-induced injury in human neuroblastoma SH-SY5Y cells.

不同提取方法对大果木姜子挥发油成分及其抑菌活性的影响

比较超声提取(UE)、微波提取(ME)、索氏提取(XZ)、水蒸气蒸馏(SD)提取的大果木姜子挥发油的化学成分,并考察其对大肠杆菌(Eco)、金黄色葡萄球菌(Sta)、枯草芽孢杆菌(Bs)、铜绿假单胞菌(Pak)的抑菌能力。结果表明:SD、UE、ME、XZ提取的大果木姜子挥发油分别鉴定出69、65、72、60种成分,相对含量分别占其总挥发油的91.382%、88.323%、85.709%、89.864%。4种挥发油对Eco的最小抑菌浓度(MIC)为SD<XZ=UE=ME,对Sta的MIC为SD<XZ=UE<ME,对Bs的MIC为XZ=UE<ME<SD,对Pak的MIC为ME<XZ=UE<SD;4种挥发油对4种供试菌的抑菌效果均高于甲醇。相关性分析表明,醇类和烃类的含量与Bs的抑制作用呈正相关。

大果木姜子挥发油的提取工艺优化、成分分析及抗氧化活性

为研究大果木姜子挥发油的最优提取工艺及其成分组成和抗氧化活性,以大果木姜子挥发油得率为指标,在单因素实验基础上,采用响应面法对提取工艺进行优化;采用气相色谱-质谱法(GC-MS)分析挥发油的组成;运用清除DPPH·试验初步评价大果木姜子挥发油的体外抗氧化活性。结果表明,最佳提取工艺为浸泡时间5 h,料液比1:5 g/mL,提取时间8 h,在上述条件下,实际得率为10.67%。从大果木姜子挥发油中鉴定出74个成分,占挥发油总量的93.175%,其中相对含量高于3%的分别为1,8-桉叶素(21.854%)、正癸酸(12.893%)、β-蒎烯(4.873%)、对伞花烃(4.579%)、α-蒎烯(4.452%)、月桂酸(3.734%)、α-萜品烯醇(3.188%);大果木姜子挥发油抗氧化活性随着浓度的增加而逐渐升高,当浓度在1.0~6.0 mg/mL范围内时,最高清除率为92.94%。优化的工艺适用于大果木姜子挥发油的提取,大果木姜子挥发油中含有多种成分,其中以萜类、烃类及酸类成分为主,抗氧化结果提示其具有一定的体外抗氧化能力。

大果木姜子化学成分、药理作用与产业化研究进展

大果木姜子是贵州省十大苗药之一,为苗族、布依族民间常用药材,主要用于治疗心律失常、心血管疾病等。近年来,大果木姜子化学成分和药理作用文献报道较少,也未有新产品问世。本文对国内外大果木姜子化学成分、药理作用和相关产业的研究进行梳理,旨在为其今后的研究和新产品的开发提供参考。

大果木姜子主要挥发性成分防治冠心病的分子机制研究

目的:采用网络药理学分析大果木姜子主要挥发性成分治疗冠心病的分子机制。方法:通过顶空固相微萃取技术分析贵州大果木姜子挥发性成分,统计相对含量大于5%挥发性成分进行靶点筛选,通过Swiss Target Prediction对主要挥发性成分进行靶点筛选,在GeneCards数据库中以“Coronary heart disease”为关键词检索冠心病疾病靶点,基于R中clusterProfiler包进行GO(Gene ontology)和KEGG(Kyoto encyclopedia of genes and genomes)富集分析,通过STRING数据库检索靶点可能的潜在相互作用。结果:桧烯(15.54%)、桉树油(6.50%)、β-石竹烯(10.04%)相对含量大于5%,共筛选出126个靶点,基于GeneCards筛选出冠心病靶点1709个,通过FunRich取交集,筛选出大果木姜子治疗冠心病靶点66个,主要通过PPAR信号通路(PPAR signaling pathway)防治冠心病。通过蛋白相互作用分析发现alpha-2A肾上腺素能受体(Alpha-2A adrenergic receptor,ADRA2A)、alpha-2B肾上腺素能受体(Alpha-2B adrenergic receptor,ADRA2B)、Alpha-2C肾上腺素能受体(Alpha-2C adrenergic receptor,ADRA2C)、过氧化物异构酶体增殖子激活受体(Peroxisome proliferator-activated receptor gamma,PPAR-γ)为关键靶点。结论:大果木姜子主要挥发性成分防治冠心病的关键靶点为ADRA2A、ADRA2B、ADRA2C、PPAR-γ。

5种特色苗药品种的研究进展与文献分析

苗药与藏、蒙、维、傣、彝并称为六大民族药,其中常用药物289种,经筛选与再评价研究,已开发上百种苗药药材及成方制剂,但收集苗药的相关专著比较零散,无系统整理。文章首次梳理了苗药研究的主要专著,并重点对头花蓼、吉祥草、大果木姜子、艾纳香和黑骨藤5种特色苗药品种的资源分布和成方制剂进行归纳总结。通过分析近10年相关中英文文献、专利及基金资助情况,讨论苗药发展现状与存在问题,为其资源的可持续利用和深入研发提供依据。