Seventeen morphological and anatomical characteristics of the leaves were selected from five natural populations to explore the variation in leaf traits of Lits ea core ana var.sinensis and the effects of geographical...Seventeen morphological and anatomical characteristics of the leaves were selected from five natural populations to explore the variation in leaf traits of Lits ea core ana var.sinensis and the effects of geographical environment on these variations.Nested analysis of variance,multiple comparisons,principal component analysis(PCA),and correlation analysis were conducted to explore the variations within and between populations and their correlation with geographical and climatic factors.Significant differences in the 17 leaf traits were observed within and among populations.On average,the relative contribution of within population variation to total variation was 24.8%,which was lower than among population variation(54.6%).The average differentiation coefficient of the traits was 65.8%,and the average coefficient of variation 11.8%,ranging from6.7%for main vein thickness to 21.4%for petiole length.The PC A results showed that morphological characteristics were divided into two categories,and the level of variation was greater than that of leaf anatomy.Most of the leaf traits were significantly correlated with geography and climate and showed a gradual variation with longitude,latitude,and altitude.In areas with high temperatures,less rainfall,and strong seasonal rainfall,the leaves are larger,longer and thicker.This study shows that variations in leaf traits of L.coreana var.sinensis mainly come from variations among populations.The level of trait differentiation among populations is high and the level of variation within populations low.These findings help further understand leaf morphological characteristics of this species and can provide a valuable reference for the protection and sustainable utilization of this natural resource.展开更多
Studying the dynamics of plant populations is crucial to the conservation and management of endangered plants.The diameter at breast height(DBH),tree height,crown width,and environmental factors of a total of 15 plots...Studying the dynamics of plant populations is crucial to the conservation and management of endangered plants.The diameter at breast height(DBH),tree height,crown width,and environmental factors of a total of 15 plots in five populations in Guizhou Province,China were measured in this study.The method of substituting diameter at breast height for age was used to divide the population into 7 age classes.The age structure,static life table,and relationship between the population characteristics and environmental factors of the Litsea coreana Levl.var.sinensis population were studied.Moreover,the time series model was used to predict the population dynamics.Results showed that the age structure of population in Zhengan County(Population ZA)had an inverted Jshape with abundant seedlings and insufficient middle-aged and elderly individuals.The age structures of populations in Daozhen County(Population DZ),Kaiyang County(KY),Meitan County(Population MT),and Xishui County(Population XS)were spindle-shaped with few young individuals and many middle-aged and elderly individuals.The mortality rate of each population fluctuated with the increase in age class,and the populations were extremely unstable.The survival curve was close to the Deevey III type,and the degree of deviation was related to the lack of young individuals.After the age stages II,IV,and VI,the number of aged plants in the different populations initially increased then decreased,and population stability was difficult to maintain.Redundancy analysis showed that the DBH,tree height and crown width of the population decreased with the increase of annual mean temperature,and the population density was restricted by soil nutrient conditions to a certain extent.The results of this work provide scientific basis for the conservation and sustainable utilization of natural populations of L.coreana var.sinensis in Guizhou Province,China.展开更多
The developmental process of oil cells in the shoot of Litsea pungens Hemsl. has been studied with transmission electron microscopy. According to the development of the three layers of cell wall, the developmental pro...The developmental process of oil cells in the shoot of Litsea pungens Hemsl. has been studied with transmission electron microscopy. According to the development of the three layers of cell wall, the developmental process could be divided into 4 stages. In stage 1, the cell wall consisted only of a primary (the outmost) cellulose layer, which might further be divided into two substages, the oil cell initial, and the vacuolizing oil cell. During this stage, there were some small electron translucent vesicles and dark osmiophilic droplets of variant sizes in the different-shaped plastids. It was observed that some dark and gray osmiophilic materials coalesced to vacuoles in the cytoplasm. In stage 2, a lamellated suberin layer accumulated inside the primary cellulose layer. In stage 3, a thicker and looser inner cellulose wall layer was formed gradually inside the suberin layer. Some dark osmiophilic droplets have been observed in this loose inner cellulose wall layer. The plasmodesmata were blocked up and became a special structure. Then, the big vacuole, which is the oil sac, was full of osmiophilic oil. In stage 4, the oil cell became matured and the cytoplasm disintegrated. The oil sac enveloped from plasmalemma was attached to the cupule, which was formed by the protuberance of the inner cellulose wall layer into the lumen. After the maturity of oil cell, the ground cytoplasm began to disintegrate and became electron opaque or exhibited in a disordered state, and the osmiophilic oil appeared light gray.展开更多
In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill...In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. Significantly better rooting response （p ≤ 0.05） was observed with 0.1% IBA compared to control （0% IBA）. The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference （p≤0.05）. After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest （100 ± 0.00%） survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly （p≤0.05） among the treatments. Effects of three fertilizer treatments, viz. T0 （no fertilizer）, T1 （10g Urea, 20g TSP, 10g MOP dissolved in 1 L water） and T2 （10g Urea, 20g TSP, 10g MOP dissolved in 2 L water） on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher （p≤0.05） under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.展开更多
Litsea,a non-monophyletic group of the tribe Laureae(Lauraceae),plays important roles in the tropical and subtropical forests of Asia,Australia,Central and North America,and the islands of the Pacific.However,intergen...Litsea,a non-monophyletic group of the tribe Laureae(Lauraceae),plays important roles in the tropical and subtropical forests of Asia,Australia,Central and North America,and the islands of the Pacific.However,intergeneric relationships between Litsea and Laurus,Lindera,Parasassafras and Sinosassafras of the tribe Laureae remain unresolved.In this study,we present phylogenetic analyses of seven newly sequenced Litsea plastomes,together with 47 Laureae plastomes obtained from public databases,representing six genera of the Laureae.Our results highlight two highly supported monophyletic groups of Litsea taxa.One is composed of 16 Litsea taxa and two Lindera taxa.The 18 plastomes of these taxa were further compared for their gene structure,codon usage,contraction and expansion of inverted repeats,sequence repeats,divergence hotspots,and gene evolution.The complete plastome size of newly sequenced taxa varied between 152,377 bp(Litsea auriculata)and 154,117 bp(Litsea pierrei).Seven of the 16 Litsea plastomes have a pair of insertions in the IRa(trnL-trnH)and IRb(ycf2)regions.The 18 plastomes of Litsea and Lindera taxa exhibit similar gene features,codon usage,oligonucleotide repeats,and inverted repeat dynamics.The codons with the highest frequency among these taxa favored A/T endings and each of these plastomes had nine divergence hotspots,which are located in the same regions.We also identified six protein coding genes(accD,ndhJ,rbcL,rpoC2,ycf1 and ycf2)under positive selection in Litsea;these genes may play important roles in adaptation of Litsea species to various environments.展开更多
The tree of Litsea cubeba is widely spread in China, Indonesia and other part of Southeast Asia. The essential oil of Litsea cubeba (EOLC) is obtained by steam distillation from the pepper-like fruits tree Litsea cube...The tree of Litsea cubeba is widely spread in China, Indonesia and other part of Southeast Asia. The essential oil of Litsea cubeba (EOLC) is obtained by steam distillation from the pepper-like fruits tree Litsea cubeba. The EOLC consists of about 29 active compounds. Among them, citral is the main component;the content of citral is nearly 80% of the EOLC. Due to the special function group, citral is easy to react with many chemicals. Thus, EOLC is usually applied as starting material to carry out aldol condensation, reduction, and six-member ring forming reaction. The EOLC is extensively employed to synthesis of geranal nitriles, pseudonoe, ionone, methyl ionone, Vitamin E and Vitamin A. These products are broadly applied in the fields of fragrance, perfume, medicine and so on. This paper presents comprehensive utilization of EOLC as raw materials to synthesize many active chemicals.展开更多
Objective:To investigate antioxidant and antimicrobial activities of two plant species,Litsea elliptica(L elliptica) and Litsea resinosa(L resinosa).Methods:In vitro method-2,2-diphenyl-1-picrylhydrazyl radical scaven...Objective:To investigate antioxidant and antimicrobial activities of two plant species,Litsea elliptica(L elliptica) and Litsea resinosa(L resinosa).Methods:In vitro method-2,2-diphenyl-1-picrylhydrazyl radical scavenging assay was conducted for antioxidant activity detennination while antimicrobial assay consisted of agar well diffusion assay and mycelial radial growth assay.Results:Methanol extracts of root and stem of L elliptica and L resinosa exhibited the highest antioxidant activity with EC_(50)of 23.99,41.69,11.22 and 33.48 mg/l.respectively.All methanol extracts of L resinosa as well as root extracts from L elliptica showed significant scavenging activity.Hexane extract from stem of L resinosa presented the largest inhibition zone in Gramnegative bacteria Pseudomonas aeruginosa and Escherichia coli while chloroform extract from inner bark of L resinosa showed major inhibition towards Gram-positive bacteria Bacillus subtilis.Essential oils from the root of both species showed significant antifungal activities which are 80.11%and 66.85%respectively.Conclusions:Overall,methanol extracts from root and stem of both species showed antioxidant activity comparable to standard butylated hydroxytoluene.Extracts from L resinosa demonstrated stronger antimicrobial properties compared to that from L elliptica.展开更多
Anticancer activity in vitro ofLitsea cubeba fruit extracts was investigated, focusing on the fruit residue from essential oil extraction. The methanol extract was fractionated by an Amberlite XAD-7 column. Cell viabi...Anticancer activity in vitro ofLitsea cubeba fruit extracts was investigated, focusing on the fruit residue from essential oil extraction. The methanol extract was fractionated by an Amberlite XAD-7 column. Cell viability, cell proliferation and cell death were determined using conversion of WST-8, BrdU incorporation and measurement of released LDH, respectively. Activation of caspase-3/-7 was detected using Z-DEVD-R substrate and morphological characteristics of apoptotic cells were revealed by DAPI staining. It was found that 80-100% methanol fractions （RME-4B, -5A, -5B and -5C） were effective against HeLa cell viability and also promoted cell death. RME-SA and -5B were highly effective in suppressing DNA replication （IC50 4.89 and 3.26 g/mL at 48 h） and also in activation of caspase-3/-7 （9 and 17 times of untreated population at 12 h）. The presence of apoptotic bodies was clearly observed. The results of this study suggested that L. cubeba fruit residue has remarkable apoptosis induction potential for further use in cancer drug research and for waste management in the essential oil industry.展开更多
The aim of the current study is to review the medicinal properties of the plants found in Brunei Darussalam namely Litsea elliptica, Dillenia suffruticosa, Dillenia excelsa, Aidia racemosa, Vitex pinnata and Senna ala...The aim of the current study is to review the medicinal properties of the plants found in Brunei Darussalam namely Litsea elliptica, Dillenia suffruticosa, Dillenia excelsa, Aidia racemosa, Vitex pinnata and Senna alata. The known phytochemical constituents of these plants and their ability to bring about a range of biological activities are included in this review. These plants have been used traditionally for a multitude of diseases and illnesses. There is a lot of untapped potential in these medicinal plants which could cure multiple diseases.展开更多
[ Objectlve] The aim of this study was to explore an explants induction method for L. cubeba. [ Method ] Different types of L. cubeba stem segments were collected at different time as explants materials for induction ...[ Objectlve] The aim of this study was to explore an explants induction method for L. cubeba. [ Method ] Different types of L. cubeba stem segments were collected at different time as explants materials for induction and culture. [ Result ] The results showed that sterilization with 0. 1% HgC12 for 10 min achieved better effects, with an explants contamination rate of dO. 0% and a mortality rate of 15.0% ; stem segments of the 2na -5th buds below the terminal bud were better explants materials for initial induction; the best sampling time was January, with an induction rate as high as 81.1%, while the contamination rate was only 11.7% ; modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA was the optimal medium for explants induction of L. cubeba. [ Conclusion] Semi-lignified stem segments of L cubeba collected in January, sterilized with 0.1% HgCI2 for 10 min and cultured in modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA a- chieved better induction effects on L. cubeba seedlings.展开更多
基金the National Natural Science Foundation of China(32060349)China Scholarship Council([2021]15)。
文摘Seventeen morphological and anatomical characteristics of the leaves were selected from five natural populations to explore the variation in leaf traits of Lits ea core ana var.sinensis and the effects of geographical environment on these variations.Nested analysis of variance,multiple comparisons,principal component analysis(PCA),and correlation analysis were conducted to explore the variations within and between populations and their correlation with geographical and climatic factors.Significant differences in the 17 leaf traits were observed within and among populations.On average,the relative contribution of within population variation to total variation was 24.8%,which was lower than among population variation(54.6%).The average differentiation coefficient of the traits was 65.8%,and the average coefficient of variation 11.8%,ranging from6.7%for main vein thickness to 21.4%for petiole length.The PC A results showed that morphological characteristics were divided into two categories,and the level of variation was greater than that of leaf anatomy.Most of the leaf traits were significantly correlated with geography and climate and showed a gradual variation with longitude,latitude,and altitude.In areas with high temperatures,less rainfall,and strong seasonal rainfall,the leaves are larger,longer and thicker.This study shows that variations in leaf traits of L.coreana var.sinensis mainly come from variations among populations.The level of trait differentiation among populations is high and the level of variation within populations low.These findings help further understand leaf morphological characteristics of this species and can provide a valuable reference for the protection and sustainable utilization of this natural resource.
基金supported by the National Natural Science Foundation of China(32060349)China Scholarship Council([2021]15)。
文摘Studying the dynamics of plant populations is crucial to the conservation and management of endangered plants.The diameter at breast height(DBH),tree height,crown width,and environmental factors of a total of 15 plots in five populations in Guizhou Province,China were measured in this study.The method of substituting diameter at breast height for age was used to divide the population into 7 age classes.The age structure,static life table,and relationship between the population characteristics and environmental factors of the Litsea coreana Levl.var.sinensis population were studied.Moreover,the time series model was used to predict the population dynamics.Results showed that the age structure of population in Zhengan County(Population ZA)had an inverted Jshape with abundant seedlings and insufficient middle-aged and elderly individuals.The age structures of populations in Daozhen County(Population DZ),Kaiyang County(KY),Meitan County(Population MT),and Xishui County(Population XS)were spindle-shaped with few young individuals and many middle-aged and elderly individuals.The mortality rate of each population fluctuated with the increase in age class,and the populations were extremely unstable.The survival curve was close to the Deevey III type,and the degree of deviation was related to the lack of young individuals.After the age stages II,IV,and VI,the number of aged plants in the different populations initially increased then decreased,and population stability was difficult to maintain.Redundancy analysis showed that the DBH,tree height and crown width of the population decreased with the increase of annual mean temperature,and the population density was restricted by soil nutrient conditions to a certain extent.The results of this work provide scientific basis for the conservation and sustainable utilization of natural populations of L.coreana var.sinensis in Guizhou Province,China.
文摘The developmental process of oil cells in the shoot of Litsea pungens Hemsl. has been studied with transmission electron microscopy. According to the development of the three layers of cell wall, the developmental process could be divided into 4 stages. In stage 1, the cell wall consisted only of a primary (the outmost) cellulose layer, which might further be divided into two substages, the oil cell initial, and the vacuolizing oil cell. During this stage, there were some small electron translucent vesicles and dark osmiophilic droplets of variant sizes in the different-shaped plastids. It was observed that some dark and gray osmiophilic materials coalesced to vacuoles in the cytoplasm. In stage 2, a lamellated suberin layer accumulated inside the primary cellulose layer. In stage 3, a thicker and looser inner cellulose wall layer was formed gradually inside the suberin layer. Some dark osmiophilic droplets have been observed in this loose inner cellulose wall layer. The plasmodesmata were blocked up and became a special structure. Then, the big vacuole, which is the oil sac, was full of osmiophilic oil. In stage 4, the oil cell became matured and the cytoplasm disintegrated. The oil sac enveloped from plasmalemma was attached to the cupule, which was formed by the protuberance of the inner cellulose wall layer into the lumen. After the maturity of oil cell, the ground cytoplasm began to disintegrate and became electron opaque or exhibited in a disordered state, and the osmiophilic oil appeared light gray.
文摘In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. Significantly better rooting response （p ≤ 0.05） was observed with 0.1% IBA compared to control （0% IBA）. The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference （p≤0.05）. After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest （100 ± 0.00%） survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly （p≤0.05） among the treatments. Effects of three fertilizer treatments, viz. T0 （no fertilizer）, T1 （10g Urea, 20g TSP, 10g MOP dissolved in 1 L water） and T2 （10g Urea, 20g TSP, 10g MOP dissolved in 2 L water） on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher （p≤0.05） under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.
基金supported by the National Natural Science Foundation of China(Grant No.32060710,31970223,31860005,31860620)Applied Basic Research Projects of Yunnan(Grant No.2019FB057).
文摘Litsea,a non-monophyletic group of the tribe Laureae(Lauraceae),plays important roles in the tropical and subtropical forests of Asia,Australia,Central and North America,and the islands of the Pacific.However,intergeneric relationships between Litsea and Laurus,Lindera,Parasassafras and Sinosassafras of the tribe Laureae remain unresolved.In this study,we present phylogenetic analyses of seven newly sequenced Litsea plastomes,together with 47 Laureae plastomes obtained from public databases,representing six genera of the Laureae.Our results highlight two highly supported monophyletic groups of Litsea taxa.One is composed of 16 Litsea taxa and two Lindera taxa.The 18 plastomes of these taxa were further compared for their gene structure,codon usage,contraction and expansion of inverted repeats,sequence repeats,divergence hotspots,and gene evolution.The complete plastome size of newly sequenced taxa varied between 152,377 bp(Litsea auriculata)and 154,117 bp(Litsea pierrei).Seven of the 16 Litsea plastomes have a pair of insertions in the IRa(trnL-trnH)and IRb(ycf2)regions.The 18 plastomes of Litsea and Lindera taxa exhibit similar gene features,codon usage,oligonucleotide repeats,and inverted repeat dynamics.The codons with the highest frequency among these taxa favored A/T endings and each of these plastomes had nine divergence hotspots,which are located in the same regions.We also identified six protein coding genes(accD,ndhJ,rbcL,rpoC2,ycf1 and ycf2)under positive selection in Litsea;these genes may play important roles in adaptation of Litsea species to various environments.
文摘The tree of Litsea cubeba is widely spread in China, Indonesia and other part of Southeast Asia. The essential oil of Litsea cubeba (EOLC) is obtained by steam distillation from the pepper-like fruits tree Litsea cubeba. The EOLC consists of about 29 active compounds. Among them, citral is the main component;the content of citral is nearly 80% of the EOLC. Due to the special function group, citral is easy to react with many chemicals. Thus, EOLC is usually applied as starting material to carry out aldol condensation, reduction, and six-member ring forming reaction. The EOLC is extensively employed to synthesis of geranal nitriles, pseudonoe, ionone, methyl ionone, Vitamin E and Vitamin A. These products are broadly applied in the fields of fragrance, perfume, medicine and so on. This paper presents comprehensive utilization of EOLC as raw materials to synthesize many active chemicals.
基金Funded by Universiti Malaysia Sarawak(UNIMAS)research grant E14052-F07-49-792/2011(2)scholarship to the postgraduate by Universiti Teknologi MARA(UITM)
文摘Objective:To investigate antioxidant and antimicrobial activities of two plant species,Litsea elliptica(L elliptica) and Litsea resinosa(L resinosa).Methods:In vitro method-2,2-diphenyl-1-picrylhydrazyl radical scavenging assay was conducted for antioxidant activity detennination while antimicrobial assay consisted of agar well diffusion assay and mycelial radial growth assay.Results:Methanol extracts of root and stem of L elliptica and L resinosa exhibited the highest antioxidant activity with EC_(50)of 23.99,41.69,11.22 and 33.48 mg/l.respectively.All methanol extracts of L resinosa as well as root extracts from L elliptica showed significant scavenging activity.Hexane extract from stem of L resinosa presented the largest inhibition zone in Gramnegative bacteria Pseudomonas aeruginosa and Escherichia coli while chloroform extract from inner bark of L resinosa showed major inhibition towards Gram-positive bacteria Bacillus subtilis.Essential oils from the root of both species showed significant antifungal activities which are 80.11%and 66.85%respectively.Conclusions:Overall,methanol extracts from root and stem of both species showed antioxidant activity comparable to standard butylated hydroxytoluene.Extracts from L resinosa demonstrated stronger antimicrobial properties compared to that from L elliptica.
文摘Anticancer activity in vitro ofLitsea cubeba fruit extracts was investigated, focusing on the fruit residue from essential oil extraction. The methanol extract was fractionated by an Amberlite XAD-7 column. Cell viability, cell proliferation and cell death were determined using conversion of WST-8, BrdU incorporation and measurement of released LDH, respectively. Activation of caspase-3/-7 was detected using Z-DEVD-R substrate and morphological characteristics of apoptotic cells were revealed by DAPI staining. It was found that 80-100% methanol fractions （RME-4B, -5A, -5B and -5C） were effective against HeLa cell viability and also promoted cell death. RME-SA and -5B were highly effective in suppressing DNA replication （IC50 4.89 and 3.26 g/mL at 48 h） and also in activation of caspase-3/-7 （9 and 17 times of untreated population at 12 h）. The presence of apoptotic bodies was clearly observed. The results of this study suggested that L. cubeba fruit residue has remarkable apoptosis induction potential for further use in cancer drug research and for waste management in the essential oil industry.
基金Supported by Universiti Brunei Darussalam and the Brunei Research Council(Grant No.JPKE/UBD/BRC6)
文摘The aim of the current study is to review the medicinal properties of the plants found in Brunei Darussalam namely Litsea elliptica, Dillenia suffruticosa, Dillenia excelsa, Aidia racemosa, Vitex pinnata and Senna alata. The known phytochemical constituents of these plants and their ability to bring about a range of biological activities are included in this review. These plants have been used traditionally for a multitude of diseases and illnesses. There is a lot of untapped potential in these medicinal plants which could cure multiple diseases.
文摘[ Objectlve] The aim of this study was to explore an explants induction method for L. cubeba. [ Method ] Different types of L. cubeba stem segments were collected at different time as explants materials for induction and culture. [ Result ] The results showed that sterilization with 0. 1% HgC12 for 10 min achieved better effects, with an explants contamination rate of dO. 0% and a mortality rate of 15.0% ; stem segments of the 2na -5th buds below the terminal bud were better explants materials for initial induction; the best sampling time was January, with an induction rate as high as 81.1%, while the contamination rate was only 11.7% ; modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA was the optimal medium for explants induction of L. cubeba. [ Conclusion] Semi-lignified stem segments of L cubeba collected in January, sterilized with 0.1% HgCI2 for 10 min and cultured in modified MS ＋ 1.0 mg/L of 6-BA ＋ 0.2 mg/L of NAA a- chieved better induction effects on L. cubeba seedlings.
