In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor poly...In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots(cDNA-POSSPQDs)were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen quantification.In this system,bar 1 was labeled with the S.T.and V.P.Apts,and then bar 2 was functionalized with cDNA-POSS-PQDs.When S.T.and V.P.were introduced,pathogen-Apt complexes would form and be released into the supernatant from bar 1.Under agitation,the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs,which were immobilized on MXene.Then,the encoded probes would be detached from bar 2 to generate FL signals in the supernatant.Notably,the pathogens can resume their free state and initiate next cycle.They swim between the two bars,and the FL signals can be gradually enhanced to maximum after several cycles.The FL signals from released encoded probes can be used to detect the analytes.In particular,live pathogens can be distinguished from dead ones by using an assay.The detection limits and linear range for S.T.and V.P.were 30 and 10 CFU/mL and 10^(2) -10^(6) CFU/mL,respectively.Therefore,this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.展开更多
目的研究双歧杆菌三联活菌辅助蓝光照射对新生儿黄疸的价值。方法选取南京市高淳人民医院于2022年5月—2023年7月收治的136例黄疸新生儿为研究对象,以随机数表法分为两组,各68例。对照组采用蓝光照射治疗,研究组在对照组基础上采用双歧...目的研究双歧杆菌三联活菌辅助蓝光照射对新生儿黄疸的价值。方法选取南京市高淳人民医院于2022年5月—2023年7月收治的136例黄疸新生儿为研究对象,以随机数表法分为两组,各68例。对照组采用蓝光照射治疗,研究组在对照组基础上采用双歧杆菌三联活菌治疗。治疗前及治疗6 d后,比较两组血清总胆红素水平(Total Serum Bilirubin,TSB)、直接胆红素(Direct Bilirubin,DBIL)、经皮黄疸指数、总胆红素与白蛋白比值(Bilirubin to Albumin Ratio,TSB/ALB)、乳酸脱氢酶水平(Lactate Dehydrogenase,LDH)水平以及不良反应发生情况。结果治疗前,两组机体胆红素水平比较,差异无统计学意义(P>0.05);治疗后,研究组TSB、DBIL及经皮黄疸指数水平低于对照组,差异有统计学意义(P均<0.05)。治疗前,两组TSB/ALB及LDH水平比较,差异无统计学意义(P均>0.05);治疗后,研究组TSB/ALB及LDH水平低于对照组,差异有统计学意义(P均<0.05)。研究组不良反应发生率为2.94%,与对照组的4.41%对比,差异无统计学意义(χ^(2)=0.208,P=0.649)。结论新生儿黄疸使用双歧杆菌三联活菌辅助蓝光照射能够降低胆红素水平,减少胆红素脑病发生风险,且不会增加不良反应。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.:21974074)Ningbo Public Welfare Technology Plan Project of China(Grant Nos.:2021Z056,2022Z170,2022S011,and 202002N3112)+2 种基金Zhejiang Provincial Top Discipline of Biological Engineering(Level A)(Grant Nos.:CX2021051 and KF2021004)Zhejiang Province Public Welfare Technology Application Research Analysis Test Plan(Grant No.:LGC20B 050006)K.C.Wong Magna Fund in Ningbo University.
文摘In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots(cDNA-POSSPQDs)were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen quantification.In this system,bar 1 was labeled with the S.T.and V.P.Apts,and then bar 2 was functionalized with cDNA-POSS-PQDs.When S.T.and V.P.were introduced,pathogen-Apt complexes would form and be released into the supernatant from bar 1.Under agitation,the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs,which were immobilized on MXene.Then,the encoded probes would be detached from bar 2 to generate FL signals in the supernatant.Notably,the pathogens can resume their free state and initiate next cycle.They swim between the two bars,and the FL signals can be gradually enhanced to maximum after several cycles.The FL signals from released encoded probes can be used to detect the analytes.In particular,live pathogens can be distinguished from dead ones by using an assay.The detection limits and linear range for S.T.and V.P.were 30 and 10 CFU/mL and 10^(2) -10^(6) CFU/mL,respectively.Therefore,this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.
文摘目的研究双歧杆菌三联活菌辅助蓝光照射对新生儿黄疸的价值。方法选取南京市高淳人民医院于2022年5月—2023年7月收治的136例黄疸新生儿为研究对象,以随机数表法分为两组,各68例。对照组采用蓝光照射治疗,研究组在对照组基础上采用双歧杆菌三联活菌治疗。治疗前及治疗6 d后,比较两组血清总胆红素水平(Total Serum Bilirubin,TSB)、直接胆红素(Direct Bilirubin,DBIL)、经皮黄疸指数、总胆红素与白蛋白比值(Bilirubin to Albumin Ratio,TSB/ALB)、乳酸脱氢酶水平(Lactate Dehydrogenase,LDH)水平以及不良反应发生情况。结果治疗前,两组机体胆红素水平比较,差异无统计学意义(P>0.05);治疗后,研究组TSB、DBIL及经皮黄疸指数水平低于对照组,差异有统计学意义(P均<0.05)。治疗前,两组TSB/ALB及LDH水平比较,差异无统计学意义(P均>0.05);治疗后,研究组TSB/ALB及LDH水平低于对照组,差异有统计学意义(P均<0.05)。研究组不良反应发生率为2.94%,与对照组的4.41%对比,差异无统计学意义(χ^(2)=0.208,P=0.649)。结论新生儿黄疸使用双歧杆菌三联活菌辅助蓝光照射能够降低胆红素水平,减少胆红素脑病发生风险,且不会增加不良反应。
