巢式RT-PCR定量检测肝癌、癌旁组织及正常肝组织内AFPmRNA

目的 :探讨肝癌、癌旁组织及正常肝组织内AFPmRNA的表达含量。方法 :应用巢式RT PCR定量检测肝癌、癌旁组织及正常肝组织内AFPmRNA。结果 :17例AFP(+ )癌旁组织内AFPmRNA表达强阳性( ) ,其癌旁组织内AFPmRNA表达呈中度阳性 ( )。 15例AFP(- )肝癌、癌旁组织及正常肝组织内AFPmR NA表达弱阳性 (+ )。结论 :AFP(+ )肝癌组织过量表达AFPmRNA基因 ,正常肝组织仅微弱表达AFPmRNA。

Changes of AFPmRNA in blood after percutaneous ethanol injection treatment of hepatocellular carcinoma

Objective: To study the effects of percutaneous etha- nol injection (PEI) in treatment of hepatocellular carcinoma (HCC). Methods: Blood samples were taken from 9 HCC pa- tients and detected by nested RT-PCR to find out AFPmRNA after PEI treatment 3-5 times within 2 weeks. Results: Three (44.44%) of 4 patients with AFPmR- NA positive turned to be negative after PEI treatment and 1 with AFPmRNA positive (11.11%) lasted within 2 weeks. Conclusion: PEI may diminish the possibility of dis- tant metastasis of HCC.

原发性肝癌组织VEGF与外周血AFP mRNA检测的临床意义

目的 探讨肝癌组织中血管内皮生长因子 (VEGF)蛋白表达与外周血液中甲胎蛋白 (AFP)mRNA表达在原发性肝细胞癌转移中的意义。方法 用巢式逆转录PCR法检测 2 0例肝癌患者术前外周血液中的AFPmRNA ,对其手术切除标本采用免疫组化方法检测VEGF蛋白表达 ,结合临床病理资料进行配对分析。结果 (1) 2 0例原发性肝细胞癌中 10例VEGF为阳性表达 ,其表达情况与临床病理参数无明显相关 ;(2 ) 2 0例肝癌中 13例AFP≥ 2 0 0 μg/L ,15例外周血液中AFPmRNA阳性 ;(3 )VEGF阳性的 10例病例中有 9例在外周血中可检测到AFPmRNA。结论 (1)肝癌组织VEGF和外周血中AFPmRNA检测都可作为判断原发性肝癌有无血行转移倾向的指标 ,前者较为简单 ,而后者较为客观准确。 (2 )对肝癌高危人群的普查和肝癌术后随访 ,应用外周血中AFPmRNA的检测方法较血清AFP水平更加敏感。

Expression of AFPmRAN in blood of patients with recurrent hepatocellular carcinoma

OBJECTIVES: To detect the expression of human alpha-fetoprotein mRNA (AFPmRNA) in peripheral blood (PBL) of recurrent hepatocellular carcinoma (HCC) by using nested reverse transcriptase polymerase chain reaction (nested RT-PCR) and to discuss relations between AFPmRNA and recurrent HCC. METHODS: Five ml peripheral blood samples were obtained from 19 patients with recurrent HCC. To identify HCC cells in PBL, liver-specific AFPmRNA was amplified from the total RNA extracted from the whole blood by nested RT-PCR. RESULTS: Seven patients (36.8%) with recurrent HCC were detected AFPmRNA, and 12 patients with recurrent HCC were AFPmRNA negative (63.2%). There was no significant relation between patients with AFPmRNA positive and AFPmRNA negative (P>0.05) in judging the origin of recurrent HCC. CONCLUSION: AFPmRNA may not be used as a marker of origin of recurrent HCC.

肝癌切除手术前后周围静脉血液中AFPmRNA的变化及其意义

目的探讨肝癌切除手术前后周围静脉血液中AFPmRNA的变化及其意义。方法应用巢式RT-PCR检测术后48小时、72小时及术后1周肝癌患者周围静脉血液中AFPmRNA,并观察变化。结果28例术前血液中AFPmRNA阴性的肝癌患者中有4例术后48小时转为阳性(14.30%),但术后1周时复转阴性;而16例术前血液中AFPmRNA阳性的肝癌患者中有4例(25.00%)术后72小时转为阴性。结论手术操作可引起肿瘤细胞播散人血循环,但它不增加手术危险性。术后短期适量应用γ-干扰素或白介素2(IL-2)是必要的。