The CCCH proteins play important roles in plant growth and development,hormone response,pathogen defense and abiotic stress tolerance.However,the knowledge of their roles in thermotolerance are scarce.Here,we identifi...The CCCH proteins play important roles in plant growth and development,hormone response,pathogen defense and abiotic stress tolerance.However,the knowledge of their roles in thermotolerance are scarce.Here,we identified a heat-inducible CCCH gene LlC3H18 from lily.LIC3H18 was localized in the cytoplasm and nucleus under normal conditions,while it translocated in the cytoplasmic foci and co-located with the markers of two messenger ribonucleoprotein(mRNP)granules,processing bodies(PBs)and stress granules(SGs)under heat stress conditions,and it also exhibited RNA-binding ability.In addition,LIC3H18 exhibited transactivation activity in both yeast and plant cells.In lily and Arabidopsis,overexpression of LIC3H18 damaged their thermotolerances,and silencing of LIC3H18 in lily also impaired its thermotolerance.Similarly,Arabidopsis atc3h18 mutant also showed decreased thermotolerance.These results indicated that the appropriate expression of C3H18 was crucial for establishing thermotolerance.Further analysis found that LIC3H18 directly bound to the promoter of LIWRKY33 and activated its expression.Besides,it was found that LIMYB305 acted as an upstream factor of LlC3H18 and activated its expression.In conclusion,we demonstrated that there may be a LIMYB305-LlC3H18-LIWRKY33 regulatory module in lily that is involved in the establishment of thermotolerance and finely regulates heat stress response.展开更多
基金Open access funding provided by Shanghai Jiao Tong Universitysupported by the National Natural Science Foundation of China(32272761,31902055)+1 种基金the Fundamental Research Funds for the Central Universities(KYZZ2022004)the Project for Crop Germplasm Resources Conservation of Jiangsu(2021-SJ-011).
文摘The CCCH proteins play important roles in plant growth and development,hormone response,pathogen defense and abiotic stress tolerance.However,the knowledge of their roles in thermotolerance are scarce.Here,we identified a heat-inducible CCCH gene LlC3H18 from lily.LIC3H18 was localized in the cytoplasm and nucleus under normal conditions,while it translocated in the cytoplasmic foci and co-located with the markers of two messenger ribonucleoprotein(mRNP)granules,processing bodies(PBs)and stress granules(SGs)under heat stress conditions,and it also exhibited RNA-binding ability.In addition,LIC3H18 exhibited transactivation activity in both yeast and plant cells.In lily and Arabidopsis,overexpression of LIC3H18 damaged their thermotolerances,and silencing of LIC3H18 in lily also impaired its thermotolerance.Similarly,Arabidopsis atc3h18 mutant also showed decreased thermotolerance.These results indicated that the appropriate expression of C3H18 was crucial for establishing thermotolerance.Further analysis found that LIC3H18 directly bound to the promoter of LIWRKY33 and activated its expression.Besides,it was found that LIMYB305 acted as an upstream factor of LlC3H18 and activated its expression.In conclusion,we demonstrated that there may be a LIMYB305-LlC3H18-LIWRKY33 regulatory module in lily that is involved in the establishment of thermotolerance and finely regulates heat stress response.
