Cloning,localization and expression analysis of two fw2.2-like genes in small-and large-fruited pear species

Fruit size is one of the most important agronomic characters,which is mainly determined by cell number and cell size.However,our current knowledge about pear is largely unknown.Through counting of pear mesocarp cells at different stages,we found that the cell number,rather than the cell size,is responsible for the differences between small- and large-fruited cultivars.Fruit weight-2.2（fw2.2） is an important quantitative trait locus（QTL） affecting fruit weight in tomato and functions as a negative regulator in carpel cell division.To get more insights into this QTL in pear fruit development,we isolated two putative homologous fw2.2 genes,which were designated as fw2.2-like（PbFWL） genes.PbFWLs encode Cys-rich proteins with the CCXXXXCPC motif and belong to the PLAC8 superfamily.In addition,results from the subcellular localization indicated that PbFWLs were localized in the plasma membrane.The expression profile of the PbFWL genes by qRT-PCR showed they expressed higher in small-sized fruit cultivar than that in large-sized fruit cultivar during the cell division period.In summary,our data suggest that these two PbFWLs might be negatively related to the cell division in pear fruit.

CD93 and GIPC expression and localization during central nervous system inflammation

CD93 and GAIP-interacting protein, C termius （GIPC） have been shown to interactively alter phagocytic processes of immune cells. CD93 and GIPC expression and localization during cen-tral nervous system inflammation have not yet been reported. In this study, we established a rat model of brain inlfammation by lipopolysaccharide injection to the lateral ventricle. In the brain of rats with inlfammation, western blots showed increased CD93 expression that decreased over time. GIPC expression was unaltered. Immunohistochemistry demonstrated extensive distribution of CD93 expression mainly in cell membranes in the cerebral cortex. After lipopoly-saccharide stimulation, CD93 expression increased and then reduced, with distinct staining in the cytoplasm and nucleus. Double immunolfuorescence staining in cerebral cortex of normal rats showed that CD93 and GIPC widely expressed in resting microglia and neurons. CD93 was mainly expressed in microglial and neuronal cell membranes, while GIPC was expressed in both cell membrane and cytoplasm. In the cerebral cortex at 9 hours after model establishment, CD93-immunoreactive signal diminished in microglial membrane, with cytoplasmic transloca-tion and aggregation detected. GIPC localization was unaltered in neurons and microglia. These results are the ifrst to demonstrate CD93 participation in pathophysiological processes of central nervous system inlfammation.

Facial expression recognition with contextualized histograms

A new algorithm taking the spatial context of local features into account by utilizing contextualized histograms was proposed to recognize facial expression. The contextualized histograms were extracted fromtwo widely used descriptors—the local binary pattern（ LBP） and weber local descriptor（ WLD）. The LBP and WLD feature histograms were extracted separately fromeach facial image,and contextualized histogram was generated as feature vectors to feed the classifier. In addition,the human face was divided into sub-blocks and each sub-block was assigned different weights by their different contributions to the intensity of facial expressions to improve the recognition rate. With the support vector machine（SVM） as classifier,the experimental results on the 2D texture images fromthe 3D-BU FE dataset indicated that contextualized histograms improved facial expression recognition performance when local features were employed.

曹禺剧作表现主义的本土化特征

曹禺的戏剧创作虽然吸收了大量西方表现主义文学流派的营养,但他的剧作始终浸润在中国传统文化之中,在确立主题和表现艺术上处处呈现出本土化的特征。对传统文化内涵的传承,对中国古代戏曲表现手法的借鉴,以及在艺术审美上追求的"诗化"风格,使曹禺剧作在表现主义特征之外打上了鲜明的中国文化的烙印。

由写实到表现:戏曲现代戏中萌动的表现意识

依照戏曲发展的整体脉络,现代戏萌动着表现主义的当代意识。表现主义直接揭示人物内心,呈现心等理空间的幻梦,为舞台的假定性、表演的虚拟性、舞台的抽象化、叙事的歌舞化提供了广阔的表现空间。环视当下的戏曲现代戏探索,现实主义戏剧观由于直观而真实,在地方戏曲现代戏中影响深远,当代依然不乏豫剧《焦裕禄》那样洋溢着现实主义迷人气质的代表之作,但舞台上萌动着的表现意识也是颇为引人瞩目的风景。

A Multiscale Model of Mass-Functionally Graded Beam-Fluid System Under Bending and Vibration Responses

In this paper,a multiscale model is developed for the mass functionally graded(FG)beam-fluid system to investigate its static and dynamic responses based on 3D printed porous beam free vibration tests,which are determined by two aspects.At the microstructural level,the gradient variation is realized by arbitrary distribution of matrix pores,and the effective moduli under specific distribution are obtained using the micromechanics homogenization theory.In the meantime,at the structural level,the mechanical responses of FG porous beams subjected to mass loading are considered in a static fluid environment.Then,the explicit expressions of local finite-element(FE)expressions corresponding to the static and dynamic responses are given in the appendices.The present results are validated against numerical and experimental results from the literature and mechanical tests of 3D printed structures,with good agreement generally obtained,giving credence to the present model.On this basis,a comprehensive parametric study is carried out,with a particular focus on the effects of boundary conditions,fluid density,and slenderness ratio on the bending and vibration of FG beams with several different gradations.