随着物联网技术的快速发展,低功率广域网络(Low-Power Wide-Area Network,LPWAN)以其特有的优势得到广泛的应用。在众多的LPWAN技术应用场景中,地磁作为无线传感器被应用于各种停车管理的场景中,其中,国内停车行业关注度最高的是远距离...随着物联网技术的快速发展,低功率广域网络(Low-Power Wide-Area Network,LPWAN)以其特有的优势得到广泛的应用。在众多的LPWAN技术应用场景中,地磁作为无线传感器被应用于各种停车管理的场景中,其中,国内停车行业关注度最高的是远距离无线电(Long Range Radio,Lo Ra)和窄带物联网(Narrow Band Internet of Things,NB-Io T)两种通信技术的地磁。本文旨在从地磁的Lo Ra与NB-Io T通信技术方面进行系统的梳理和比较分析,为地磁的选择应用提供有力的技术参考和储备。展开更多
On the morning of Oct 12,2017,a representative office of France’s Seine-et-Marne department in the country’s Ile-de-France region was opened in Bailu Music Townof Pengzhou,a county-level city under the jurisdiction ...On the morning of Oct 12,2017,a representative office of France’s Seine-et-Marne department in the country’s Ile-de-France region was opened in Bailu Music Townof Pengzhou,a county-level city under the jurisdiction of Chengdu.Appropriately,the street was French-styled,and melodious music filled the air.Jean-Jacques Barbaux,president of Seine-展开更多
西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制...西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制率、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)泄漏量越大,相同乙醇浓度下,红西凤和食用酒精(对照组)干预组之间具有显著性差异(P<0.05)。动物实验结果表明,不同剂量红西凤和食用酒精干预均导致小鼠体重、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)含量降低,肝重体重比、AST、ALT、TC、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)含量升高,肝脏组织出现不同程度脂肪变性,肠道菌群发生明显变化(门水平上,厚壁菌门与拟杆菌门比例下降;属水平上,乳酸杆菌属、毛螺菌属等相对丰度降低,幽门螺杆菌属、链球菌属等相对丰度增加),LEfSe分析结果显示,小鼠肠道菌群存在的差异与红西凤和食用酒精干预剂量有一定关系,且高剂量干预对小鼠肠道菌群的影响有扩大的趋势,然而同剂量下,红西凤干预对小鼠的损伤程度轻于食用酒精,以低剂量红西凤干预对小鼠的影响最轻,推测红西凤中各微量成分可能通过调节小鼠肠道菌群从而减缓对肝脏的损伤程度。以上结果说明,适量摄入红西凤在一定程度上可以缓解酒精对LO2细胞及小鼠肠道菌群的负面影响,该研究将为西凤酒的健康消费提供理论依据。展开更多
Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocreba...Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.展开更多
The Tencoi Gyur Ro Tsel Ki Kar Chak Thub Ten Gyes Pe Nyi Oi(Bttan bcos vgyur ro vtshal gyi dkar chag thub bstan rgyas pavi nyivod in Tibetan),generally refers to Lo Manthang Tengyur Catalogue in the circle of the acad...The Tencoi Gyur Ro Tsel Ki Kar Chak Thub Ten Gyes Pe Nyi Oi(Bttan bcos vgyur ro vtshal gyi dkar chag thub bstan rgyas pavi nyivod in Tibetan),generally refers to Lo Manthang Tengyur Catalogue in the circle of the academy,is a Tengyur Catalogue supported by Drungchen Agon Sangpo(Drung chen a mgon bzang po in Tibetan)of Lo Manthang(glo smon thang in Tibetan).This catalog is little known in U-tsang area even though it bears an important value in the field of Tengyur studies.Your author of this article,using the compilation of this Lo Manthang Tengyur as an entry point,conducts an academic study on the time,the place,and the supporter of the compilation of Lo Manthang Tengyur,and discusses the compiler of the catalogue,which has been heavily controversial in the academic circle.Your author rectifies that the compiler of Lo Manthang Tengyur was Sakya Gelong Kunga Sangpo(sa skyavi dge slong kun dgav bzang po in Tibetan).At the same time,a comparative study of this Tengyur catalogue with Narthang Tengyur Catalogue by Wupa Losei(dbus pa blo gsal in Tibetan),the Shalu Tengyur Catalogue by Buton Rinchendrub(bu ston rin chen sgrub in Tibetan),and the Tshalpa Tengyur Catalogue compiled during the period of the Tsepa Trungchen Monlam Dorje(Tshal pa drung chen smon lam rdo rje in Tibetan)is carried out in general.Particularly,your author finds that the ways of classifying sections and chapters share similarities with Tsepa Tengyur Catalogue while the ways of categorifying the titles of the classical works and names of writers and translators display a unique characteristic.展开更多
Length-weight relationships and condition factors are among the commonly used parameters to link the length of a fish to its weight. The present study was initiated to find out the growth patterns and overweight statu...Length-weight relationships and condition factors are among the commonly used parameters to link the length of a fish to its weight. The present study was initiated to find out the growth patterns and overweight status of 15 fish species in the Loémé basin in Mayombe, Republic of Congo. In the period from February 2020 to September 2021, sampling was carried out in eight stations, following a standardised capture method using four gillnets of 10, 12, 15, and 25 mm mesh size and a hawk net of 1.90 m radius with 10 mm mesh size. Length-weight relationships were calculated according to P = aLS<sup>b</sup> and the condition factor, using K = (P/LS<sup>b</sup>)* 100. A total of 1153 specimens representing 15 species, 10 genera and 6 families were recorded. The number of specimens ranged from 10 to 214 for Chrysichthys auratus and Enteromius camptacanthus, respectively. The allometry coefficient b minimum of 2.697 for Chromidotilapiamelaniae and maximum of 3.589 for Garraornata, with a mean of 3.03 ± 0.2 is significantly not different from 3 at the 0.05 probability threshold and indicates isometric growth for almost all species, except for E. camptacanthus where b = 2.858 was statistically different from 3 at the same threshold. The positive and high values of the coefficients of determination r<sup>2</sup> = 0.787-0.989 of the species, obtained during this study, suggest that weight increases with size in the fish of the Loémé basin. The average condition factor varies from 0.524 ± 0.064 for G. ornata to 4.917 ± 0.440 for C. melaniae.展开更多
文摘随着物联网技术的快速发展,低功率广域网络(Low-Power Wide-Area Network,LPWAN)以其特有的优势得到广泛的应用。在众多的LPWAN技术应用场景中,地磁作为无线传感器被应用于各种停车管理的场景中,其中,国内停车行业关注度最高的是远距离无线电(Long Range Radio,Lo Ra)和窄带物联网(Narrow Band Internet of Things,NB-Io T)两种通信技术的地磁。本文旨在从地磁的Lo Ra与NB-Io T通信技术方面进行系统的梳理和比较分析,为地磁的选择应用提供有力的技术参考和储备。
文摘On the morning of Oct 12,2017,a representative office of France’s Seine-et-Marne department in the country’s Ile-de-France region was opened in Bailu Music Townof Pengzhou,a county-level city under the jurisdiction of Chengdu.Appropriately,the street was French-styled,and melodious music filled the air.Jean-Jacques Barbaux,president of Seine-
文摘西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制率、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)泄漏量越大,相同乙醇浓度下,红西凤和食用酒精(对照组)干预组之间具有显著性差异(P<0.05)。动物实验结果表明,不同剂量红西凤和食用酒精干预均导致小鼠体重、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)含量降低,肝重体重比、AST、ALT、TC、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)含量升高,肝脏组织出现不同程度脂肪变性,肠道菌群发生明显变化(门水平上,厚壁菌门与拟杆菌门比例下降;属水平上,乳酸杆菌属、毛螺菌属等相对丰度降低,幽门螺杆菌属、链球菌属等相对丰度增加),LEfSe分析结果显示,小鼠肠道菌群存在的差异与红西凤和食用酒精干预剂量有一定关系,且高剂量干预对小鼠肠道菌群的影响有扩大的趋势,然而同剂量下,红西凤干预对小鼠的损伤程度轻于食用酒精,以低剂量红西凤干预对小鼠的影响最轻,推测红西凤中各微量成分可能通过调节小鼠肠道菌群从而减缓对肝脏的损伤程度。以上结果说明,适量摄入红西凤在一定程度上可以缓解酒精对LO2细胞及小鼠肠道菌群的负面影响,该研究将为西凤酒的健康消费提供理论依据。
基金Natural Science Foundation of Hainan Province(No.820RC776)。
文摘Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.
文摘The Tencoi Gyur Ro Tsel Ki Kar Chak Thub Ten Gyes Pe Nyi Oi(Bttan bcos vgyur ro vtshal gyi dkar chag thub bstan rgyas pavi nyivod in Tibetan),generally refers to Lo Manthang Tengyur Catalogue in the circle of the academy,is a Tengyur Catalogue supported by Drungchen Agon Sangpo(Drung chen a mgon bzang po in Tibetan)of Lo Manthang(glo smon thang in Tibetan).This catalog is little known in U-tsang area even though it bears an important value in the field of Tengyur studies.Your author of this article,using the compilation of this Lo Manthang Tengyur as an entry point,conducts an academic study on the time,the place,and the supporter of the compilation of Lo Manthang Tengyur,and discusses the compiler of the catalogue,which has been heavily controversial in the academic circle.Your author rectifies that the compiler of Lo Manthang Tengyur was Sakya Gelong Kunga Sangpo(sa skyavi dge slong kun dgav bzang po in Tibetan).At the same time,a comparative study of this Tengyur catalogue with Narthang Tengyur Catalogue by Wupa Losei(dbus pa blo gsal in Tibetan),the Shalu Tengyur Catalogue by Buton Rinchendrub(bu ston rin chen sgrub in Tibetan),and the Tshalpa Tengyur Catalogue compiled during the period of the Tsepa Trungchen Monlam Dorje(Tshal pa drung chen smon lam rdo rje in Tibetan)is carried out in general.Particularly,your author finds that the ways of classifying sections and chapters share similarities with Tsepa Tengyur Catalogue while the ways of categorifying the titles of the classical works and names of writers and translators display a unique characteristic.
文摘Length-weight relationships and condition factors are among the commonly used parameters to link the length of a fish to its weight. The present study was initiated to find out the growth patterns and overweight status of 15 fish species in the Loémé basin in Mayombe, Republic of Congo. In the period from February 2020 to September 2021, sampling was carried out in eight stations, following a standardised capture method using four gillnets of 10, 12, 15, and 25 mm mesh size and a hawk net of 1.90 m radius with 10 mm mesh size. Length-weight relationships were calculated according to P = aLS<sup>b</sup> and the condition factor, using K = (P/LS<sup>b</sup>)* 100. A total of 1153 specimens representing 15 species, 10 genera and 6 families were recorded. The number of specimens ranged from 10 to 214 for Chrysichthys auratus and Enteromius camptacanthus, respectively. The allometry coefficient b minimum of 2.697 for Chromidotilapiamelaniae and maximum of 3.589 for Garraornata, with a mean of 3.03 ± 0.2 is significantly not different from 3 at the 0.05 probability threshold and indicates isometric growth for almost all species, except for E. camptacanthus where b = 2.858 was statistically different from 3 at the same threshold. The positive and high values of the coefficients of determination r<sup>2</sup> = 0.787-0.989 of the species, obtained during this study, suggest that weight increases with size in the fish of the Loémé basin. The average condition factor varies from 0.524 ± 0.064 for G. ornata to 4.917 ± 0.440 for C. melaniae.
