An Analysis of Long Day’s Journey into Night from the Perspective of the Cooperative Principle

Eugene O’Neill,one of the essential writers of the 20th century,was hailed as the“American Shakespeare.”He enjoyed a high reputation in America owing to his special writing techniques.Long Day’s Journey into Night was his last play,which was an autobiographical play to some extent,and founded on his own authentic experience.The play told the story of an Irish immigrant family’s daily life from day to night.Among the studies at home and abroad,many discuss this play from a psychological perspective and Feminist perspective.The article,instead,gives an interpretation of this play according to Cooperative Principle.Through the analysis of their conversations,it points out what principles their communications violated so as to understand the implied meaning of their conversations.The thesis tries to study the conversations between Mary and other family members,and then demonstrate that Mary is the most tragic person in the family.

Low temperature-mediated repression and far-red light-mediated induction determine morning FLOWERING LOCUS T expression levels

In order to flower in the appropriate season,plants monitor light and temperature changes and alter downstream pathways that regulate florigen genes such as Arabidopsis(Arabidopsis thaliana)FLOWERING LOCUS T(FT).In Arabidopsis,FT messenger RNA levels peak in the morning and evening under natural long-day conditions(LDs).However,the regulatory mechanisms governing morning FT induction remain poorly understood.The morning FT peak is absent in typical laboratory LDs characterized by high red:far-red light(R:FR)ratios and constant temperatures.Here,we demonstrate that ZEITLUPE(ZTL)interacts with the FT repressors TARGET OF EATs(TOEs),thereby repressing morning FT expression in natural environments.Under LDs with simulated sunlight(R:FR=1.0)and daily temperature cycles,which are natural LD-mimicking environmental conditions,FT transcript levels in the ztl mutant were high specifically in the morning,a pattern that was mirrored in the toe1 toe2 double mutant.Low night-to-morning temperatures increased the inhibitory effect of ZTL on morning FT expression by increasing ZTL protein levels early in the morning.Far-red light counteracted ZTL activity by decreasing its abundance(possibly via phytochrome A(phyA))while increasing GIGANTEA(GI)levels and negatively affecting the formation of the ZTL-GI complex in the morning.Therefore,the phyA-mediated high-irradiance response and GI play pivotal roles in morning FT induction.Our findings suggest that the delicate balance between low temperature-mediated ZTL activity and the far-red light-mediated functions of phyA and GI offers plants flexibility in fine-tuning their flowering time by controlling FT expression in the morning.