The transcriptional factor GATA-6 gene produces two translational isoforms from a single mRNA through ribosomal leaky scanning. L-type GATA-6 has an extension of 146 amino acid residues at its amino terminus. In the e...The transcriptional factor GATA-6 gene produces two translational isoforms from a single mRNA through ribosomal leaky scanning. L-type GATA-6 has an extension of 146 amino acid residues at its amino terminus. In the extension, there is a unique PEST sequence (Glu31-Cys46), which is composed of an amino terminal Pro-rich segment and a carboxyl terminal Ser-cluster. Substitution of either half of the PEST sequence with Ala residues by cassette mutagenesis reduced the apparent molecular size of L-type GATA-6 on SDS-polyacrylamide gel-electrophoresis. However, the effect of substitution of the Pro-rich segment was much more significant;the mobility increase of the Pro-rich segment on the gel was 13% while that of the Ser-cluster was 8%. Substitution of each amino acid residue demonstrated that the effect of Pro substitution is greater than that of the Ser and Thr residues. Such increased mobility of L-type GATA-6 in the presence of a detergent may apparently correlate with the decrease in transcription activity in vivo as determined by means of luciferase reporter gene assay. The activity of ΔAla (with Ala residues instead of the PEST sequence) was reduced to one fifth of that of ΔA (with the PEST sequence). These results suggest that the PEST sequence of L-type GATA-6 does not function as a constitutive protein degradation signal, but rather plays structural and functional roles in the activation of gene expression on the GATA responsive promoter.展开更多
中国生态系统研究网络(Chinese Ecosystem Research Network,CERN)生物长期样地背景和植被分类特征本底数据集是22个CERN自然生态系统生态站95个长期样地的本底数据的综合集成。基于对CERN生态站长期样地地理位置、建立时间、面积、样...中国生态系统研究网络(Chinese Ecosystem Research Network,CERN)生物长期样地背景和植被分类特征本底数据集是22个CERN自然生态系统生态站95个长期样地的本底数据的综合集成。基于对CERN生态站长期样地地理位置、建立时间、面积、样地代表性等背景信息的梳理,依据样地建立之初的植物群落调查数据,参照最新的中国植被分类系统,对每个样地植物群落所属的植被型组、植被型、植被亚型进行了明确划分,并依据每个样地的优势种名单,对样地植被所属群系和群丛进行了鉴定和命名。本数据集构建通过了多轮的专家审核-台站核查-专家复审-台站接受等过程,包含生态站代码、生态站名称、样地代码、样地名称、样地类别、样地代表性、地理位置、海拔高度、样地面积及形状、样地建立时间和设计使用年数、植被型组、植被型、植被亚型、群系、群丛、优势种等信息。本数据集可以为植物区系、植被资源分布、生物多样性保护等方面的研究提供基础数据支持。展开更多
文摘The transcriptional factor GATA-6 gene produces two translational isoforms from a single mRNA through ribosomal leaky scanning. L-type GATA-6 has an extension of 146 amino acid residues at its amino terminus. In the extension, there is a unique PEST sequence (Glu31-Cys46), which is composed of an amino terminal Pro-rich segment and a carboxyl terminal Ser-cluster. Substitution of either half of the PEST sequence with Ala residues by cassette mutagenesis reduced the apparent molecular size of L-type GATA-6 on SDS-polyacrylamide gel-electrophoresis. However, the effect of substitution of the Pro-rich segment was much more significant;the mobility increase of the Pro-rich segment on the gel was 13% while that of the Ser-cluster was 8%. Substitution of each amino acid residue demonstrated that the effect of Pro substitution is greater than that of the Ser and Thr residues. Such increased mobility of L-type GATA-6 in the presence of a detergent may apparently correlate with the decrease in transcription activity in vivo as determined by means of luciferase reporter gene assay. The activity of ΔAla (with Ala residues instead of the PEST sequence) was reduced to one fifth of that of ΔA (with the PEST sequence). These results suggest that the PEST sequence of L-type GATA-6 does not function as a constitutive protein degradation signal, but rather plays structural and functional roles in the activation of gene expression on the GATA responsive promoter.
文摘中国生态系统研究网络(Chinese Ecosystem Research Network,CERN)生物长期样地背景和植被分类特征本底数据集是22个CERN自然生态系统生态站95个长期样地的本底数据的综合集成。基于对CERN生态站长期样地地理位置、建立时间、面积、样地代表性等背景信息的梳理,依据样地建立之初的植物群落调查数据,参照最新的中国植被分类系统,对每个样地植物群落所属的植被型组、植被型、植被亚型进行了明确划分,并依据每个样地的优势种名单,对样地植被所属群系和群丛进行了鉴定和命名。本数据集构建通过了多轮的专家审核-台站核查-专家复审-台站接受等过程,包含生态站代码、生态站名称、样地代码、样地名称、样地类别、样地代表性、地理位置、海拔高度、样地面积及形状、样地建立时间和设计使用年数、植被型组、植被型、植被亚型、群系、群丛、优势种等信息。本数据集可以为植物区系、植被资源分布、生物多样性保护等方面的研究提供基础数据支持。
文摘目的 分析2型糖尿病(T2DM)采取长效胰岛素+口服降糖药治疗的效果。方法 96例T2DM患者,随机分为观察组和对照组,每组48例。对照组给予口服降糖药治疗,观察组给予长效胰岛素+口服降糖药治疗。比较两组患者治疗前后的血糖[糖化血红蛋白(HbA1c)、餐后2 h血糖(2 h PG)、空腹血糖(FPG)]水平、生活质量(总体健康、精力、社会功能、心理健康、躯体疼痛、躯体角色功能、情绪角色功能、躯体健康)评分以及不良反应发生情况(体重增加、低血糖)、治疗效果。结果 治疗后,观察组患者HbA1c(6.20±0.91)%、2 h PG(8.63±1.37)mmol/L、FPG(5.83±0.72)mmol/L均低于对照组的(8.34±1.15)%、(9.56±1.60)mmol/L、(7.91±1.31)mmol/L,差异具有统计学意义(P<0.05)。观察组不良反应发生率2.08%(1/48)低于对照组的14.58%(7/48),差异具有统计学意义(χ^(2)=4.909,P<0.05)。治疗后,观察组患者总体健康评分(63.25±4.48)分、精力评分(74.25±4.54)分、社会功能评分(83.67±3.91)分、心理健康评分(81.48±4.25)分、躯体疼痛评分(74.48±6.38)分、躯体角色功能评分(73.54±4.18)分、情绪角色功能评分(84.54±3.67)分、躯体健康评分(71.37±4.28)分;对照组患者总体健康评分(54.54±3.25)分、精力评分(61.22±4.01)分、社会功能评分(76.32±2.75)分、心理健康评分(61.24±3.75)分、躯体疼痛评分(66.58±4.70)分、躯体角色功能评分(61.24±4.42)分、情绪角色功能评分(73.05±2.50)分、躯体健康评分(52.31±2.76)分。观察组患者总体健康、精力、社会功能、心理健康、躯体疼痛、躯体角色功能、情绪角色功能、躯体健康评分均高于对照组,差异有统计学意义(P<0.05)。观察组治疗总有效率95.83%高于对照组的79.17%,差异具有统计学意义(P<0.05)。结论 长效胰岛素联合口服降糖药物对T2DM治疗可有效改善血糖水平,降低不良反应发生率,提升疗效。
