Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproper...Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproperties of high sliming paste from a copper mine in China.Based on the rheological properties test,the pressure and velocity of pipeline and elbow are simulated by CFD under different mass concentrations and different stowing capacities.The results show that the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with high sliming and pumping agent at the same mass concentration,and the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with low sliming while without pumping agent.It is very important to add pumping agent to whole-tailings paste with high sliming,and the resistance changes with mass concentration and stowing capacity at the same cement-to-sand ratio of1:5and tailings-to-waste ratio of6:1.However,the change is just limited,that is to say,the paste transportation system is of good stability.Furthermore,at the elbow,the maximum pressure and velocity transfer to the outside of the pipe from the inside.However,lubricating layer is formed at the pipe wall because of high content of fine particles in whole-tailings paste,which will protect the elbow from abrasion.CFD provides an intuitive and accurate basis for pipeline transportation study,and would have a wider application space in the study of paste rheological properties and resistance reduction methods.展开更多
BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified...BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3' untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3' untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.展开更多
Nicotinamide adenine dinucleotide (NAD) biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate (NA) via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-ci...Nicotinamide adenine dinucleotide (NAD) biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate (NA) via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-cies of NA conjugates, which are mainly involved in NA detoxification, were also found in all tested land plants. Among these conjugates, MeNA (NA methyl ester) has been widely detected in angiosperm plants, although its physiological function and the underlying mechanism for its production in planta remain largely unknown. Here, we show that MeNA is an NAD precursor undergoing more efficient long-distance trans-port between organs than NA and nicotinamide in Arabidopsis. We found that Arabidopsis has one meth- yltransferase (designated AtNaMT1) capable of catalyzing carboxyl methylation of NA to yield MeNA and one methyl esterase (MES2) predominantly hydrolyzing MeNA back to NA. We further uncovered that the transfer of [^14C]MeNA from the root to leaf was significantly increased in both MES2 knockdown and NaMTl-overexpressing lines, suggesting that both NaMT1 and MES2 fine-tune the long-distance transport of MeNA, which is ultimately utilized for NAD production. Abiotic stress (salt, abscisic acid, and mannitol) treatments, which are known to exacerbate NAD degradation, induce the expression of NaMT1 but sup-press MES2 expression, suggesting that MeNA may play a role in stress adaption. Collectively, our study indicates that reversible methylation of NA controls the biosynthesis of MeNA in Arabidopsis, which pre-sumably functions as a detoxification form of free NA for efficient long-distance transport and eventually NAD production especially under abiotic stress, providing new insights into the relationship between NAD biosynthesis and NA conjugation in plants.展开更多
Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress responses.Long-distance transport of tZ-type CKs meidated by Arabidopsis ATP...Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress responses.Long-distance transport of tZ-type CKs meidated by Arabidopsis ATP-binding cassette transporter subfamily G14(AtABCG14)has been well studied;however,less is known about the biochemical properties of AtABCG14 and its transporter activity toward iP-type CKs.Here we reveal the biochemical properties of AtABCG14 and provide evidence that it is also required for long-distance transport of iP-type CKs.AtABCG14 formed homodimers in human(Homo sapiens)HEK293T,tobacco(Nicotiana tabacum),and Arabidopsis cells.Transporter activity assays of AtABCG14 in Arabidopsis,tobacco,and yeast(Saccharomyces cerevisiae)showed that AtABCG14 may directly transport multiple CKs,including iP-and tZ-type species.AtABCG14 expression was induced by iP in a tZ-type CKdeficient double mutant(cypDM)of CYP735A1 and CYP735A2.The atabcg14 cypDM triple mutant exhibited stronger CK-deficiency phenotypes than cypDM.Hormone profiling,reciprocal grafting,and 2H6-iP isotope tracer experiments showed that root-to-shoot and shoot-to-root long-distance transport of iP-type CKs were suppressed in atabcg14 cypDM and atabcg14.These results suggest that AtABCG14 participates in three steps of the circular long-distance transport of iP-type CKs:xylem loading in the root for shootward transport,phloem unloading in the shoot for shoot distribution,and phloemunloading in the root for root distribution.We found that AtABCG14 displays transporter activity toward multiple CK species and revealed its versatile roles in circular long-distance transport of iP-type CKs.These findings provide newinsights into the transport mechanisms of CKs and other plant hormones.展开更多
Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexib...Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexible pipes and further diffuse into the annular space,incurring annular pressure build-up and/or production of acidic environment,which poses serious challenges to the structure and integrity of the flexible pipes.Gas permeation in pipes is a complex phenomenon governed by various factors such as internal pressure and temperature,annular structure,external temperature.In a long-distance gas flexible pipe,moreover,gas permeation exhibits non-uniform features,and the gas permeated into the annular space flows along the metal gap.To assess the complex gas transport behavior in long-distance gas flexible pipes,a mathematical model is established in this paper considering the multiphase flow phenomena inside the flexible pipes,the diffusion of gas in the inner liner,and the gas seepage in the annular space under varying permeable properties of the annulus.In addition,the effect of a variable temperature is accounted.A numerical calculation method is accordingly constructed to solve the coupling mathematical equations.The annular permeability was shown to significantly influence the distribution of annular pressure.As permeability increases,the annular pressure tends to become more uniform,and the annular pressure at the wellhead rises more rapidly.After annular pressure relief followed by shut-in,the pressure increase follows a convex function.By simulating the pressure recovery pattern after pressure relief and comparing it with test results,we deduce that the annular permeability lies between 123 and 512 m D.The results help shed light upon assessing the annular pressure in long distance gas flexible pipes and thus ensure the security of gas transport in the emerging development of offshore resources.展开更多
Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechani...Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.展开更多
Iron(Fe)transport and reallocation are essential to Fe homeostasis in plants,but it is unclear how Fe homeostasis is regulated,especially under stress.Here we report that NPF5.9 and its close homolog NPF5.8 redundantl...Iron(Fe)transport and reallocation are essential to Fe homeostasis in plants,but it is unclear how Fe homeostasis is regulated,especially under stress.Here we report that NPF5.9 and its close homolog NPF5.8 redundantly regulate Fe transport and reallocation in Arabidopsis.NPF5.9 is highly upregulated in response to Fe deficiency.NPF5.9 expresses preferentially in vasculature tissues and localizes to the trans-Golgi network,and NPF5.8 showed a similar expression pattern.Long-distance Fe transport and allocation into aerial parts was significantly increased in NPF5.9-overexpressing lines.In the double mutant npf5.8 npf5.9,Fe loading in aerial parts and plant growth were decreased,which were partially rescued by Fe supplementation.Further analysis showed that expression of PYE,the negative regulator for Fe homeostasis,and its downstream target NAS4 were significantly altered in the double mutant.NPF5.9 and NPF5.8 were shown to also mediate nitrate uptake and transport,although nitrate and Fe application did not reciprocally affect each other.Our findings uncovered the novel function of NPF5.9 and NPF5.8 in long-distance Fe transport and homeostasis,and further indicated that they possibly mediate nitrate transport and Fe homeostasis independently in Arabidopsis.展开更多
In this study,the Weather Research and Forecasting(WRF)model and meteorological observation data were used to research the long-distance moisture transport supply source of the extreme rainfall event that occurred on ...In this study,the Weather Research and Forecasting(WRF)model and meteorological observation data were used to research the long-distance moisture transport supply source of the extreme rainfall event that occurred on July 21,2012 in Beijing.Recording a maximum rainfall amount of 460 mm in 24 h,this rainstorm event had two dominant moisture transport channels.In the early stage of the rainstorm,the first channel comprised southwesterly monsoonal moisture from the Bay of Bengal(BOB)that was directly transported to north China along the eastern edge of Tibetan Plateau(TP)by orographic uplift.During the rainstorm,the southwesterly moisture transport was weakened by the transfer of Typhoon Vicente.Moreover,the southeasterly moisture transport between the typhoon and western Pacific subtropical high(WPSH)became another dominant moisture transport channel.The moisture in the lower troposphere was mainly associated with the southeasterly moisture transport from the South China Sea and the East China Sea,and the moisture in the middle troposphere was mainly transported from the BOB and Indian Ocean.The control experiment well reproduced the distribution and intensity of rainfall and moisture transport.By comparing the control and three sensitivity experiments,we found that the moisture transported from Typhoon Vicente and a tropical cyclone in the BOB both significantly affected this extreme rainfall event.After Typhoon Vicente was removed in a sensitivity experiment,the maximum 24-h accumulated rainfall in north China was reduced by approximately 50%compared with that of the control experiment,while the rainfall after removing the tropical cyclone was reduced by 30%.When both the typhoon and tropical cyclone were removed,the southwesterly moisture transport was enhanced.Moreover,the sensitivity experiment of removing Typhoon Vicente also weakened the tropical cyclone in the BOB.Thus,the moisture pump driven by Typhoon Vicente played an important role in maintaining and strengthening the tropical cyclone in the BOB through its westerly airflow.Typhoon Vicente was not only the moisture transfer source for the southwesterly monsoonal moisture but also affected the tropical cyclone in the BOB,which was a key supply source of long-distance moisture transport for the extreme rainfall event on July21,2012 in Beijing.展开更多
The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of mana...The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of managing personal moisture/thermal comfort in response to changing external environments remains a challenge.Herein,a smart cellulose-based Janus fabric was designed to dynamically manage personal moisture/heat.The cotton fabric was grafted with N-isopropylacrylamide to construct a temperature-stimulated transport channel.Subsequently,hydrophobic ethyl cellulose and hydrophilic cellulose nanofiber were sprayed on the bottom and top sides of the fabric to obtain wettability gradient.The fabric exhibits anti-gravity directional liquid transportation from hydrophobic side to hydrophilic side,and can dynamically and continuously control the transportation time in a wide range of 3–66 s as the temperature increases from 10 to 40℃.This smart fabric can quickly dissipate heat at high temperatures,while at low temperatures,it can slow down the heat dissipation rate and prevent the human from becoming too cold.In addition,the fabric has UV shielding and photodynamic antibacterial properties through depositing graphitic carbon nitride nanosheets on the hydrophilic side.This smart fabric offers an innovative approach to maximizing personal comfort in environments with significant temperature variations.展开更多
The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain functio...The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.展开更多
This paper attempts to reveal a long-distance-relayed water vapor transport(LRWVT) east of Tibetan Plateau and its impacts. The results show that from August to October, east of Tibetan Plateau, there exists a unique ...This paper attempts to reveal a long-distance-relayed water vapor transport(LRWVT) east of Tibetan Plateau and its impacts. The results show that from August to October, east of Tibetan Plateau, there exists a unique LRWVT,and the water vapor from the South China Sea and the western Pacific can affect the Sichuan Basin, Northwest China and other Chinese regions far from the tropical sea through this way. From August to October, the precipitation of the region east of the Plateau is closely linked both in the intra-annual and inter-annual variations, and the LRWVT from the South China Sea and the western Pacific is an important connection mechanism. The large-scale circulation background of the LRWVT impacting the precipitation of the region east of the Plateau is as follows: At high levels,the South Asian High is generally stronger than normal and significantly enhances with its northward advance and eastward extension over the region east of the Plateau. At mid-level, a broad low pressure trough is over Lake Balkhash and its surroundings, and the Western Pacific Subtropical High(WPSH) is northward and westward located, and the western part of Sichuan Basin and the eastern part of Northwest China are located in the west and northwest edge of WPSH.展开更多
In addition to the loss of motor function,~60% of patients develop pain after spinal cord injury.The cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epice...In addition to the loss of motor function,~60% of patients develop pain after spinal cord injury.The cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epicenter,and caudal penumbra of the injury site initiates a cellularmolecular interplay that acts as a rewiring mechanism leading to central neuropathic pain.Sprouting can lead to the formation of new connections triggering abnormal sensory transmission.The excitatory glutamate transporters are responsible for the reuptake of extracellular glutamate which makes them a critical target to prevent neuronal hyperexcitability and excitotoxicity.Our previous studies showed a sexually dimorphic therapeutic window for spinal cord injury after treatment with the selective estrogen receptor modulator tamoxifen.In this study,we investigated the anti-allodynic effects of tamoxifen in male and female rats with spinal cord injury.We hypothesized that tamoxifen exerts anti-allodynic effects by increasing the expression of glutamate transporters,leading to reduced hyperexcitability of the secondary neuron or by decreasing aberrant sprouting.Male and female rats received a moderate contusion to the thoracic spinal cord followed by subcutaneous slow-release treatment of tamoxifen or matrix pellets as a control(placebo).We used von Frey monofilaments and the“up-down method”to evaluate mechanical allodynia.Tamoxifen treatment decreased allodynia only in female rats with spinal cord injury revealing a sexdependent effect.The expression profile of glutamatergic transporters(excitatory amino acid transporter 1/glutamate aspartate transporter and excitatory amino acid transporter 2/glutamate transporter-1)revealed a sexual dimorphism in the rostral,epicenter,and caudal areas of the spinal cord with a pattern of expression primarily on astrocytes.Female rodents showed a significantly higher level of excitatory amino acid transporter-1 expression while male rodents showed increased excitatory amino acid transporter-2 expression compared with female rodents.Analyses of peptidergic(calcitonin gene-related peptide-α)and non-peptidergic(isolectin B4)fibers outgrowth in the dorsal horn after spinal cord injury showed an increased calcitonin gene-related peptide-α/isolectin B4 ratio in comparison with sham,suggesting increased receptive fields in the dorsal horn.Although the behavioral assay shows decreased allodynia in tamoxifen-treated female rats,this was not associated with overexpression of glutamate transporters or alterations in the dorsal horn laminae fibers at 28 days post-injury.Our findings provide new evidence of the sexually dimorphic expression of glutamate transporters in the spinal cord.The dimorphic expression revealed in this study provides a therapeutic opportunity for treating chronic pain,an area with a critical need for treatment.展开更多
For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention....For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up.展开更多
The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, ...The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, it would be necessary to establish a system of information management for the pipeline. The monitoring, calculating and analyzing functions of the system serve to give controlling instructions and safe operating rules to the automatic equipment and technician, making sure the resistance coefficient distribution along the pipeline is reasonable; the hydraulic state transition is smooth when operating conditions change or water supply accidents occur, avoiding the damage of water hammer. This paper covered the composition structures of the information management system of long-distance water transmission pipelines and the functions of the subsystems, and finally elaborated on the approaches and steps of building a mathematics model for the analysis of dynamic hydraulic status.展开更多
The limited physical size for autonomous underwater vehicles (AUV) or unmanned underwater vehicles (UUV) makes it difficult to acquire enough space gain for localizing long-distance targets. A new technique about ...The limited physical size for autonomous underwater vehicles (AUV) or unmanned underwater vehicles (UUV) makes it difficult to acquire enough space gain for localizing long-distance targets. A new technique about long-distance target apperception with passive synthetic aperture array for underwater vehicles is presented. First, a synthetic aperture-processing algorithm based on the FFT transform in the beam space (BSSAP) is introduced. Then, the study on the flank array passive long-distance apperception techniques in the frequency scope of 11-18 kHz is implemented from the view of improving array gains, detection probability and augmenting detected range under a certain sea environment. The results show that the BSSAP algorithm can extend the aperture effectively and improve detection probability. Because of the augment of the transmission loss, the detected range has the trend of decline with the increase of frequency under the same target source level. The synthesized array could improve the space gain by nearly 7 dB and SNR is increased by about 5 dB. The detected range is enhanced to nearly 2 km under the condition of 108-118 dB of the target source level for AUV system in measurement interval of nearly 1 s.展开更多
Propagation properties of spatially pseudo-partially coherent Gaussian Schell-model beams through the atmo- spheric turbulence over a long-distance uplink path are studied by numerical simulation. A linear coordinatio...Propagation properties of spatially pseudo-partially coherent Gaussian Schell-model beams through the atmo- spheric turbulence over a long-distance uplink path are studied by numerical simulation. A linear coordination trans- formation is introduced to overcome the window effect and the loss-of-resolution problem. The beam spreading, beam wandering, and intensity scintillation as functions of turbulence strength, source correlation length, and change fre- quency of random phase that models the partial coherence of the source are analyzed. It is found that the beam spreading and the intensity scintillation of the partially coherent beam are less affected by the turbulence than those of the coherent one, but it suffers from a more severe diffractive effect, and the change frequency of random phase has no evident influence on it. The beam wandering is insensitive to the variation of source correlation length, and decreases firstly then goes to a fixed value as the change frequency increases.展开更多
in order to verify the heat-tolerance effect, two trainings, 90 min marching with load (WBGT 24. 6~35.6℃) and 10 km running (WBGT 25.0~31.1℃) were performed in laboratory and field under hot climate.Ten to twelve ...in order to verify the heat-tolerance effect, two trainings, 90 min marching with load (WBGT 24. 6~35.6℃) and 10 km running (WBGT 25.0~31.1℃) were performed in laboratory and field under hot climate.Ten to twelve times (days) of training were carried out展开更多
Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, pr...Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, previous studies focused on species dispersed via animal-mediated LDD, and ignored those dispersed by wind. The aim of this study was to assess the effects of canopy openness, edge, seed source, and patch tree density on the LDD of seeds by wind in forest. We collected birch seeds, a typical wind-dispersed species, throughout a larch plantation. We then assessed the relationship between birch LDD and each factor that may influence LDD of seeds by wind including distance to edge, canopy openness size, distance to mature forest, and the tree density of the larch plantation. We used univariate linear regression analysis to assess the influence of those factors on birch LDD, and partial correlations to calculate the contribution of each factor to LDD. The results showed that both canopy openness and edge had significant influences on birch LDD. Specifically, a negative relationship was observed between distance to edge and birch LDD, whereas there was a positive correlation between canopy openness size and LDD. In contrast, the distance to the mature forest was not correlated with birch LDD. Our results suggest that patch tree density could potently affect the probability of LDD by wind vectors, which provides novel and revealing insights regarding the effect of fragmentation on wind dynamics. The data also provide compelling evidence for the previously undocumented effect of habitat fragmentation on wind-dispersed organisms. As such, these observations will facilitate reasonable conservation planning, which requires a detailed understanding of the mechanisms by which patch properties hamper the delivery of seeds of wind-dispersed plants to fragmented areas.展开更多
According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows: the flat landform is the first choice in route alignment. The fo...According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows: the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment.展开更多
A steel underground pipeline with a diameter of 2.4 m and a total length of 3,617 m(plate thickness of 26 mm)has been constructed in a city in central Hubei,and the engineering,procurement,and construction(EPC)project...A steel underground pipeline with a diameter of 2.4 m and a total length of 3,617 m(plate thickness of 26 mm)has been constructed in a city in central Hubei,and the engineering,procurement,and construction(EPC)project has been lifted from the upstream channel to supplement water to the downstream lake inside the city.Through preliminary geological survey data,site topographic and geomorphic survey,urban construction,as well as the requirements of the construction party,the preliminary arrangement of working wells and receiving wells as well as the selection and customization of pipe jacking machines have been proposed.Frequency conversion motor and remote monitoring technology are adopted for geotechnical change and long-distance pipe jacking.Through detailed survey,the rock and soil change section as well as gradual change conditions have been determined,the accuracy of construction mechanics calculation and construction operation control have improved,the basis and analysis basis are provided,and some experiences in construction operation are summarized.展开更多
基金Project(2016YFC0600709)supported by the National Key R&D Program of ChinaProject(51574013)supported by the National Natural Science Foundation of ChinaProject(FRF-TP-17-024A1)supported by the Fundamental Research Funds for the Central Universities,China
文摘Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproperties of high sliming paste from a copper mine in China.Based on the rheological properties test,the pressure and velocity of pipeline and elbow are simulated by CFD under different mass concentrations and different stowing capacities.The results show that the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with high sliming and pumping agent at the same mass concentration,and the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with low sliming while without pumping agent.It is very important to add pumping agent to whole-tailings paste with high sliming,and the resistance changes with mass concentration and stowing capacity at the same cement-to-sand ratio of1:5and tailings-to-waste ratio of6:1.However,the change is just limited,that is to say,the paste transportation system is of good stability.Furthermore,at the elbow,the maximum pressure and velocity transfer to the outside of the pipe from the inside.However,lubricating layer is formed at the pipe wall because of high content of fine particles in whole-tailings paste,which will protect the elbow from abrasion.CFD provides an intuitive and accurate basis for pipeline transportation study,and would have a wider application space in the study of paste rheological properties and resistance reduction methods.
基金supported by the United States Departmentof Agriculture NRI-CGP award no.2008-02806National Science Foundation-Plant Genome Research Program award no.0820659
文摘BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3' untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3' untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.
文摘Nicotinamide adenine dinucleotide (NAD) biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate (NA) via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-cies of NA conjugates, which are mainly involved in NA detoxification, were also found in all tested land plants. Among these conjugates, MeNA (NA methyl ester) has been widely detected in angiosperm plants, although its physiological function and the underlying mechanism for its production in planta remain largely unknown. Here, we show that MeNA is an NAD precursor undergoing more efficient long-distance trans-port between organs than NA and nicotinamide in Arabidopsis. We found that Arabidopsis has one meth- yltransferase (designated AtNaMT1) capable of catalyzing carboxyl methylation of NA to yield MeNA and one methyl esterase (MES2) predominantly hydrolyzing MeNA back to NA. We further uncovered that the transfer of [^14C]MeNA from the root to leaf was significantly increased in both MES2 knockdown and NaMTl-overexpressing lines, suggesting that both NaMT1 and MES2 fine-tune the long-distance transport of MeNA, which is ultimately utilized for NAD production. Abiotic stress (salt, abscisic acid, and mannitol) treatments, which are known to exacerbate NAD degradation, induce the expression of NaMT1 but sup-press MES2 expression, suggesting that MeNA may play a role in stress adaption. Collectively, our study indicates that reversible methylation of NA controls the biosynthesis of MeNA in Arabidopsis, which pre-sumably functions as a detoxification form of free NA for efficient long-distance transport and eventually NAD production especially under abiotic stress, providing new insights into the relationship between NAD biosynthesis and NA conjugation in plants.
基金the National Natural Science Foundation of China(31470370 to K.Z.and 32100270 to J.Z.)the Natural Science Foundation of Zhejiang Province(LY22C020003 to J.Z.)C.-J.L.was supported by the DOE Office of Basic Energy Sciences,specifically through the Physical Biosciences Program of the Chemical Sciences,Geosciences and Biosciences Division,under contract DESC0012704.
文摘Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress responses.Long-distance transport of tZ-type CKs meidated by Arabidopsis ATP-binding cassette transporter subfamily G14(AtABCG14)has been well studied;however,less is known about the biochemical properties of AtABCG14 and its transporter activity toward iP-type CKs.Here we reveal the biochemical properties of AtABCG14 and provide evidence that it is also required for long-distance transport of iP-type CKs.AtABCG14 formed homodimers in human(Homo sapiens)HEK293T,tobacco(Nicotiana tabacum),and Arabidopsis cells.Transporter activity assays of AtABCG14 in Arabidopsis,tobacco,and yeast(Saccharomyces cerevisiae)showed that AtABCG14 may directly transport multiple CKs,including iP-and tZ-type species.AtABCG14 expression was induced by iP in a tZ-type CKdeficient double mutant(cypDM)of CYP735A1 and CYP735A2.The atabcg14 cypDM triple mutant exhibited stronger CK-deficiency phenotypes than cypDM.Hormone profiling,reciprocal grafting,and 2H6-iP isotope tracer experiments showed that root-to-shoot and shoot-to-root long-distance transport of iP-type CKs were suppressed in atabcg14 cypDM and atabcg14.These results suggest that AtABCG14 participates in three steps of the circular long-distance transport of iP-type CKs:xylem loading in the root for shootward transport,phloem unloading in the shoot for shoot distribution,and phloemunloading in the root for root distribution.We found that AtABCG14 displays transporter activity toward multiple CK species and revealed its versatile roles in circular long-distance transport of iP-type CKs.These findings provide newinsights into the transport mechanisms of CKs and other plant hormones.
基金supported by the Natural Science Research Project of Guangling College of Yangzhou University,China (ZKZD18004)General Program of Natural Science Research in Higher Education Institutions of Jiangsu Province,China (20KJD430006)。
文摘Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexible pipes and further diffuse into the annular space,incurring annular pressure build-up and/or production of acidic environment,which poses serious challenges to the structure and integrity of the flexible pipes.Gas permeation in pipes is a complex phenomenon governed by various factors such as internal pressure and temperature,annular structure,external temperature.In a long-distance gas flexible pipe,moreover,gas permeation exhibits non-uniform features,and the gas permeated into the annular space flows along the metal gap.To assess the complex gas transport behavior in long-distance gas flexible pipes,a mathematical model is established in this paper considering the multiphase flow phenomena inside the flexible pipes,the diffusion of gas in the inner liner,and the gas seepage in the annular space under varying permeable properties of the annulus.In addition,the effect of a variable temperature is accounted.A numerical calculation method is accordingly constructed to solve the coupling mathematical equations.The annular permeability was shown to significantly influence the distribution of annular pressure.As permeability increases,the annular pressure tends to become more uniform,and the annular pressure at the wellhead rises more rapidly.After annular pressure relief followed by shut-in,the pressure increase follows a convex function.By simulating the pressure recovery pattern after pressure relief and comparing it with test results,we deduce that the annular permeability lies between 123 and 512 m D.The results help shed light upon assessing the annular pressure in long distance gas flexible pipes and thus ensure the security of gas transport in the emerging development of offshore resources.
基金supported by the 111 Project(Grant No.B17043)the 2115 Talent Development Program of China Agricultural University。
文摘Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.
基金R&D Program of China(2016YFD0100700)in part by the Ministry of Agriculture of China for Transgenic Research(2016ZX08009003-005-003).
文摘Iron(Fe)transport and reallocation are essential to Fe homeostasis in plants,but it is unclear how Fe homeostasis is regulated,especially under stress.Here we report that NPF5.9 and its close homolog NPF5.8 redundantly regulate Fe transport and reallocation in Arabidopsis.NPF5.9 is highly upregulated in response to Fe deficiency.NPF5.9 expresses preferentially in vasculature tissues and localizes to the trans-Golgi network,and NPF5.8 showed a similar expression pattern.Long-distance Fe transport and allocation into aerial parts was significantly increased in NPF5.9-overexpressing lines.In the double mutant npf5.8 npf5.9,Fe loading in aerial parts and plant growth were decreased,which were partially rescued by Fe supplementation.Further analysis showed that expression of PYE,the negative regulator for Fe homeostasis,and its downstream target NAS4 were significantly altered in the double mutant.NPF5.9 and NPF5.8 were shown to also mediate nitrate uptake and transport,although nitrate and Fe application did not reciprocally affect each other.Our findings uncovered the novel function of NPF5.9 and NPF5.8 in long-distance Fe transport and homeostasis,and further indicated that they possibly mediate nitrate transport and Fe homeostasis independently in Arabidopsis.
基金National Natural Science Foundation of China(91937301,42030611)Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK0103,2019QZKK0105)Scientific and Technological Research Program of China Railway Eryuan Engineering Group CO.LTD(KYY2020066(20-22))
文摘In this study,the Weather Research and Forecasting(WRF)model and meteorological observation data were used to research the long-distance moisture transport supply source of the extreme rainfall event that occurred on July 21,2012 in Beijing.Recording a maximum rainfall amount of 460 mm in 24 h,this rainstorm event had two dominant moisture transport channels.In the early stage of the rainstorm,the first channel comprised southwesterly monsoonal moisture from the Bay of Bengal(BOB)that was directly transported to north China along the eastern edge of Tibetan Plateau(TP)by orographic uplift.During the rainstorm,the southwesterly moisture transport was weakened by the transfer of Typhoon Vicente.Moreover,the southeasterly moisture transport between the typhoon and western Pacific subtropical high(WPSH)became another dominant moisture transport channel.The moisture in the lower troposphere was mainly associated with the southeasterly moisture transport from the South China Sea and the East China Sea,and the moisture in the middle troposphere was mainly transported from the BOB and Indian Ocean.The control experiment well reproduced the distribution and intensity of rainfall and moisture transport.By comparing the control and three sensitivity experiments,we found that the moisture transported from Typhoon Vicente and a tropical cyclone in the BOB both significantly affected this extreme rainfall event.After Typhoon Vicente was removed in a sensitivity experiment,the maximum 24-h accumulated rainfall in north China was reduced by approximately 50%compared with that of the control experiment,while the rainfall after removing the tropical cyclone was reduced by 30%.When both the typhoon and tropical cyclone were removed,the southwesterly moisture transport was enhanced.Moreover,the sensitivity experiment of removing Typhoon Vicente also weakened the tropical cyclone in the BOB.Thus,the moisture pump driven by Typhoon Vicente played an important role in maintaining and strengthening the tropical cyclone in the BOB through its westerly airflow.Typhoon Vicente was not only the moisture transfer source for the southwesterly monsoonal moisture but also affected the tropical cyclone in the BOB,which was a key supply source of long-distance moisture transport for the extreme rainfall event on July21,2012 in Beijing.
基金support of this work by National Key Research and Development Program of China(2019YFC19059003)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(23KJB430024)+1 种基金Jiangsu Funding Program for Excellent Postdoctoral Talent(2023ZB680)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)are gratefully acknowledged.
文摘The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of managing personal moisture/thermal comfort in response to changing external environments remains a challenge.Herein,a smart cellulose-based Janus fabric was designed to dynamically manage personal moisture/heat.The cotton fabric was grafted with N-isopropylacrylamide to construct a temperature-stimulated transport channel.Subsequently,hydrophobic ethyl cellulose and hydrophilic cellulose nanofiber were sprayed on the bottom and top sides of the fabric to obtain wettability gradient.The fabric exhibits anti-gravity directional liquid transportation from hydrophobic side to hydrophilic side,and can dynamically and continuously control the transportation time in a wide range of 3–66 s as the temperature increases from 10 to 40℃.This smart fabric can quickly dissipate heat at high temperatures,while at low temperatures,it can slow down the heat dissipation rate and prevent the human from becoming too cold.In addition,the fabric has UV shielding and photodynamic antibacterial properties through depositing graphitic carbon nitride nanosheets on the hydrophilic side.This smart fabric offers an innovative approach to maximizing personal comfort in environments with significant temperature variations.
基金supported by the National Natural Science Foundation of China,Nos.32371070 (to JT),31761163005 (to JT),32100824 (to QX)the Shenzhen Science and Technology Program,Nos.RCBS20210609104606024 (to QX),JCY20210324101813035 (to DL)+4 种基金the Guangdong Provincial Key S&T Program,No.2018B030336001 (to JT)the Key Basic Research Program of Shenzhen Science and Technology Innovation Commission,Nos.JCYJ20200109115405930 (to JT),JCYJ20220818101615033 (to DL),JCYJ20210324115811031 (to QX),JCYJ20200109150717745 (to QX)Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases,No.ZDSYS20220304163558001 (to JT)Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,No.2023B1212060055 (to JT)the China Postdoctoral Science Foundation,No.2021M693298 (to QX)。
文摘The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.
基金Major Program of the National Natural Science Foundation of China(41290255)National Natural Science Foundation of China(41305082)Fundamental Research Funds of Chengdu Institute of Plateau Meteorology,CMA
文摘This paper attempts to reveal a long-distance-relayed water vapor transport(LRWVT) east of Tibetan Plateau and its impacts. The results show that from August to October, east of Tibetan Plateau, there exists a unique LRWVT,and the water vapor from the South China Sea and the western Pacific can affect the Sichuan Basin, Northwest China and other Chinese regions far from the tropical sea through this way. From August to October, the precipitation of the region east of the Plateau is closely linked both in the intra-annual and inter-annual variations, and the LRWVT from the South China Sea and the western Pacific is an important connection mechanism. The large-scale circulation background of the LRWVT impacting the precipitation of the region east of the Plateau is as follows: At high levels,the South Asian High is generally stronger than normal and significantly enhances with its northward advance and eastward extension over the region east of the Plateau. At mid-level, a broad low pressure trough is over Lake Balkhash and its surroundings, and the Western Pacific Subtropical High(WPSH) is northward and westward located, and the western part of Sichuan Basin and the eastern part of Northwest China are located in the west and northwest edge of WPSH.
基金supported by COBRE(P30GM149367)the Puerto Rico Science&Technology Trust(2022-00125)+1 种基金MBRS-RISE Program(R25 GM061838)SC1GM144032 program(all to JDM)。
文摘In addition to the loss of motor function,~60% of patients develop pain after spinal cord injury.The cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epicenter,and caudal penumbra of the injury site initiates a cellularmolecular interplay that acts as a rewiring mechanism leading to central neuropathic pain.Sprouting can lead to the formation of new connections triggering abnormal sensory transmission.The excitatory glutamate transporters are responsible for the reuptake of extracellular glutamate which makes them a critical target to prevent neuronal hyperexcitability and excitotoxicity.Our previous studies showed a sexually dimorphic therapeutic window for spinal cord injury after treatment with the selective estrogen receptor modulator tamoxifen.In this study,we investigated the anti-allodynic effects of tamoxifen in male and female rats with spinal cord injury.We hypothesized that tamoxifen exerts anti-allodynic effects by increasing the expression of glutamate transporters,leading to reduced hyperexcitability of the secondary neuron or by decreasing aberrant sprouting.Male and female rats received a moderate contusion to the thoracic spinal cord followed by subcutaneous slow-release treatment of tamoxifen or matrix pellets as a control(placebo).We used von Frey monofilaments and the“up-down method”to evaluate mechanical allodynia.Tamoxifen treatment decreased allodynia only in female rats with spinal cord injury revealing a sexdependent effect.The expression profile of glutamatergic transporters(excitatory amino acid transporter 1/glutamate aspartate transporter and excitatory amino acid transporter 2/glutamate transporter-1)revealed a sexual dimorphism in the rostral,epicenter,and caudal areas of the spinal cord with a pattern of expression primarily on astrocytes.Female rodents showed a significantly higher level of excitatory amino acid transporter-1 expression while male rodents showed increased excitatory amino acid transporter-2 expression compared with female rodents.Analyses of peptidergic(calcitonin gene-related peptide-α)and non-peptidergic(isolectin B4)fibers outgrowth in the dorsal horn after spinal cord injury showed an increased calcitonin gene-related peptide-α/isolectin B4 ratio in comparison with sham,suggesting increased receptive fields in the dorsal horn.Although the behavioral assay shows decreased allodynia in tamoxifen-treated female rats,this was not associated with overexpression of glutamate transporters or alterations in the dorsal horn laminae fibers at 28 days post-injury.Our findings provide new evidence of the sexually dimorphic expression of glutamate transporters in the spinal cord.The dimorphic expression revealed in this study provides a therapeutic opportunity for treating chronic pain,an area with a critical need for treatment.
基金supported by the National Natural Science Foundation of China(Grants No.52179062 and 51879087).
文摘For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up.
基金Hi-Tech Research and Development Program of China (863 Program)(2002AA601140)
文摘The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, it would be necessary to establish a system of information management for the pipeline. The monitoring, calculating and analyzing functions of the system serve to give controlling instructions and safe operating rules to the automatic equipment and technician, making sure the resistance coefficient distribution along the pipeline is reasonable; the hydraulic state transition is smooth when operating conditions change or water supply accidents occur, avoiding the damage of water hammer. This paper covered the composition structures of the information management system of long-distance water transmission pipelines and the functions of the subsystems, and finally elaborated on the approaches and steps of building a mathematics model for the analysis of dynamic hydraulic status.
文摘The limited physical size for autonomous underwater vehicles (AUV) or unmanned underwater vehicles (UUV) makes it difficult to acquire enough space gain for localizing long-distance targets. A new technique about long-distance target apperception with passive synthetic aperture array for underwater vehicles is presented. First, a synthetic aperture-processing algorithm based on the FFT transform in the beam space (BSSAP) is introduced. Then, the study on the flank array passive long-distance apperception techniques in the frequency scope of 11-18 kHz is implemented from the view of improving array gains, detection probability and augmenting detected range under a certain sea environment. The results show that the BSSAP algorithm can extend the aperture effectively and improve detection probability. Because of the augment of the transmission loss, the detected range has the trend of decline with the increase of frequency under the same target source level. The synthesized array could improve the space gain by nearly 7 dB and SNR is increased by about 5 dB. The detected range is enhanced to nearly 2 km under the condition of 108-118 dB of the target source level for AUV system in measurement interval of nearly 1 s.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 61107066 and 40805006)
文摘Propagation properties of spatially pseudo-partially coherent Gaussian Schell-model beams through the atmo- spheric turbulence over a long-distance uplink path are studied by numerical simulation. A linear coordination trans- formation is introduced to overcome the window effect and the loss-of-resolution problem. The beam spreading, beam wandering, and intensity scintillation as functions of turbulence strength, source correlation length, and change fre- quency of random phase that models the partial coherence of the source are analyzed. It is found that the beam spreading and the intensity scintillation of the partially coherent beam are less affected by the turbulence than those of the coherent one, but it suffers from a more severe diffractive effect, and the change frequency of random phase has no evident influence on it. The beam wandering is insensitive to the variation of source correlation length, and decreases firstly then goes to a fixed value as the change frequency increases.
文摘in order to verify the heat-tolerance effect, two trainings, 90 min marching with load (WBGT 24. 6~35.6℃) and 10 km running (WBGT 25.0~31.1℃) were performed in laboratory and field under hot climate.Ten to twelve times (days) of training were carried out
基金National Natural Science Foundation of China(No.31300526)National Key Technologies R&D Program of China(No.2012BAD22B04)Chinese Forest Ecosystem Research Network&GENE Award Funds on Ecological Paper
文摘Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, previous studies focused on species dispersed via animal-mediated LDD, and ignored those dispersed by wind. The aim of this study was to assess the effects of canopy openness, edge, seed source, and patch tree density on the LDD of seeds by wind in forest. We collected birch seeds, a typical wind-dispersed species, throughout a larch plantation. We then assessed the relationship between birch LDD and each factor that may influence LDD of seeds by wind including distance to edge, canopy openness size, distance to mature forest, and the tree density of the larch plantation. We used univariate linear regression analysis to assess the influence of those factors on birch LDD, and partial correlations to calculate the contribution of each factor to LDD. The results showed that both canopy openness and edge had significant influences on birch LDD. Specifically, a negative relationship was observed between distance to edge and birch LDD, whereas there was a positive correlation between canopy openness size and LDD. In contrast, the distance to the mature forest was not correlated with birch LDD. Our results suggest that patch tree density could potently affect the probability of LDD by wind vectors, which provides novel and revealing insights regarding the effect of fragmentation on wind dynamics. The data also provide compelling evidence for the previously undocumented effect of habitat fragmentation on wind-dispersed organisms. As such, these observations will facilitate reasonable conservation planning, which requires a detailed understanding of the mechanisms by which patch properties hamper the delivery of seeds of wind-dispersed plants to fragmented areas.
文摘According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows: the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment.
文摘A steel underground pipeline with a diameter of 2.4 m and a total length of 3,617 m(plate thickness of 26 mm)has been constructed in a city in central Hubei,and the engineering,procurement,and construction(EPC)project has been lifted from the upstream channel to supplement water to the downstream lake inside the city.Through preliminary geological survey data,site topographic and geomorphic survey,urban construction,as well as the requirements of the construction party,the preliminary arrangement of working wells and receiving wells as well as the selection and customization of pipe jacking machines have been proposed.Frequency conversion motor and remote monitoring technology are adopted for geotechnical change and long-distance pipe jacking.Through detailed survey,the rock and soil change section as well as gradual change conditions have been determined,the accuracy of construction mechanics calculation and construction operation control have improved,the basis and analysis basis are provided,and some experiences in construction operation are summarized.