[Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [Methods]With superior individuals of wild ...[Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [Methods]With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer,polyploid induction was performed twice on diploid plants by addition method,and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results]Among the 7 polyploidy plants,four materials were tetraploid,the concentration and treatment time of which were 300 mg/L and 7 and 14 d,respectively; and three materials were octaploid,the concentrations and treatment time of which were 300 and 500 mg/L and 14 d,respectively. [Conclusions]The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects,and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore,colchicine is an polyploid inducer suitable for L. edulis.展开更多
An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branch...An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A fourfactor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L-1 6-BA, 0.3 mg · L-1 IBA and 1.5 mg · L-1 GA3.展开更多
Lonicera edulis is a perennial berry shrub that prefers the cold and wet climate,with high nutritional and medicinal value. In this study,ecological coupling capability of L. edulis with light,heat,water and soil reso...Lonicera edulis is a perennial berry shrub that prefers the cold and wet climate,with high nutritional and medicinal value. In this study,ecological coupling capability of L. edulis with light,heat,water and soil resources in cold regions was investigated to analyze comprehensively ecological effects of quantitative characters and genetic effects of parents,aiming at providing a theoretical basis for the breeding,introduction and domestication of fine varieties of L. edulis in cold regions of China. The results showed that fruit characters in L. edulis exhibited certain variations among different habitat types. To be specific,fruit vertical diameter varied slightly,fruit horizontal diameter varied greatly and seed number varied maximally,with the average variance coefficients of 9. 38%,11. 92% and20. 64%,respectively; in addition,fruit characters in L. edulis exhibited moderate heritability,moderate genetic gain and low level of genetic differentiation; the heritability of fruit vertical diameter,fruit horizontal diameter and seed number was 0. 825,0. 559 and 0. 627,respectively; the genetic gain of these three fruit characters was 7. 53%,5. 72% and 11. 94%,respectively,resulting in significant economical benefits.展开更多
In order to solve the problems including low fruit-bearing rate and low yield caused by blind plantation in production,the medium formula for pollen germination of Lonicera caerulea L. var. edulis Turcz. ex Herd.,its ...In order to solve the problems including low fruit-bearing rate and low yield caused by blind plantation in production,the medium formula for pollen germination of Lonicera caerulea L. var. edulis Turcz. ex Herd.,its pollination habit,self-compatibility and open pollination compatibility between different superior individuals were investigated with superior individuals from superior wild individuals of L. caerulea in Changbai Mountains as experimental materials. Self-compatibility and open pollination compatibility among different fine plants provide theoretical basis for its further application and further selection of new varieties. The results showed the optimal medium for germination of L. caerulea pollen was 27. 5% sucrose + 100 PPM boric acid with a pH value of 6. 0. On this medium,the superior individuals L1,L2 and L3 had the pollen germination rates of 53. 4%,50. 9% and 51. 6%,respectively. The three superior individuals had no significant differences in the quantity of germinated pollen tubes,which ranged from 1 to 4. The three excellent single plants were the most likely to germinate a single pollen tube,accounting for 83. 3% of the pollen germinated and 89. 6% of the pollen germinated. Therefore,in production,it is necessary to plant L1 with a certain amount of pollination trees or plant it together with multiple species,to improve its yield. On the contrary,L2 and L3 have no need for pollination trees theoretically,but whether the fruits obtained by self-pollination and outcrossing differ in quality still needs further study.展开更多
基金Supported by The Cooperative Project between the Yanbian University,ChinaNational Institute of Horticultural and Herbal Science of the Rural Development Administration(RDA)of the Republic of Korea
文摘[Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [Methods]With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer,polyploid induction was performed twice on diploid plants by addition method,and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results]Among the 7 polyploidy plants,four materials were tetraploid,the concentration and treatment time of which were 300 mg/L and 7 and 14 d,respectively; and three materials were octaploid,the concentrations and treatment time of which were 300 and 500 mg/L and 14 d,respectively. [Conclusions]The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects,and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore,colchicine is an polyploid inducer suitable for L. edulis.
基金Supported by the State Bureau of Foreign Experts 2013 Annual Talent Introduction of Foreign Technology,Project Management(20132300046)
文摘An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A fourfactor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L-1 6-BA, 0.3 mg · L-1 IBA and 1.5 mg · L-1 GA3.
基金Supported by Project of Running Service of National Forest Tree Germplasm Resource Platform(2011DKA21003-07)Special Fund for Forest Scientific Research in the Public Welfare(201204307-07)Science and Technology Support Program of Heilongjiang Province(GB06B306,GB06B306-02)
文摘Lonicera edulis is a perennial berry shrub that prefers the cold and wet climate,with high nutritional and medicinal value. In this study,ecological coupling capability of L. edulis with light,heat,water and soil resources in cold regions was investigated to analyze comprehensively ecological effects of quantitative characters and genetic effects of parents,aiming at providing a theoretical basis for the breeding,introduction and domestication of fine varieties of L. edulis in cold regions of China. The results showed that fruit characters in L. edulis exhibited certain variations among different habitat types. To be specific,fruit vertical diameter varied slightly,fruit horizontal diameter varied greatly and seed number varied maximally,with the average variance coefficients of 9. 38%,11. 92% and20. 64%,respectively; in addition,fruit characters in L. edulis exhibited moderate heritability,moderate genetic gain and low level of genetic differentiation; the heritability of fruit vertical diameter,fruit horizontal diameter and seed number was 0. 825,0. 559 and 0. 627,respectively; the genetic gain of these three fruit characters was 7. 53%,5. 72% and 11. 94%,respectively,resulting in significant economical benefits.
基金Supported by the Cooperative Project between the Yanbian University,China and National Institute of Horticultural and Herbal Science of the Rural Development Administration(RDA)of the Republic of Korea
文摘In order to solve the problems including low fruit-bearing rate and low yield caused by blind plantation in production,the medium formula for pollen germination of Lonicera caerulea L. var. edulis Turcz. ex Herd.,its pollination habit,self-compatibility and open pollination compatibility between different superior individuals were investigated with superior individuals from superior wild individuals of L. caerulea in Changbai Mountains as experimental materials. Self-compatibility and open pollination compatibility among different fine plants provide theoretical basis for its further application and further selection of new varieties. The results showed the optimal medium for germination of L. caerulea pollen was 27. 5% sucrose + 100 PPM boric acid with a pH value of 6. 0. On this medium,the superior individuals L1,L2 and L3 had the pollen germination rates of 53. 4%,50. 9% and 51. 6%,respectively. The three superior individuals had no significant differences in the quantity of germinated pollen tubes,which ranged from 1 to 4. The three excellent single plants were the most likely to germinate a single pollen tube,accounting for 83. 3% of the pollen germinated and 89. 6% of the pollen germinated. Therefore,in production,it is necessary to plant L1 with a certain amount of pollination trees or plant it together with multiple species,to improve its yield. On the contrary,L2 and L3 have no need for pollination trees theoretically,but whether the fruits obtained by self-pollination and outcrossing differ in quality still needs further study.
