Breeding and Application Process of a New Variety‘Dianyinhua 3’in Lonicera macranthoides Hand.-Mazz.

‘Dianyinhua 3’is bred through the selection of a good single variant plant based on Hunan Lonicerae Flos variety‘Xianglei 1’as the basic material.The scions of the variant plant were grafted and propagated with wild Lonicera macranthoides Hand.-Mazz.as rootstocks.This variety belongs to L.macaroides,which has the characteristics of early flowering,large and numerous buds,concentrated flowering and abundant yield.The content of chlorogenic acid is as high as 6%,and the content of luteolin is 0.017%.It matures from late May to early June in Yunnan.The buds are rod-shaped,slightly curved,4.6-5.8 cm long,with a diameter of 6.47 mm at the top and 2.68 mm at the bottom,and the petals have a thickness of 0.33 mm.The thousand-flower bud weight was 15.8-18.2 g,and the average yield of dried flowers per plant was 1360-1680 g in the peak period,and the yield was 4887 kg/hm^2.It is suitable for the introduction and trial planting in Xiangyang Mountainous Area or rocky desertification mountainous area in Yunnan,with an altitude lower than 2200 m or in areas with similar climates such as Sichuan,Guizhou and Guangxi.

Two new triterpenoid saponins from Lonicera macranthoides

Two new triterpenoid saponins named lonimacranthoideⅣ（1） and lonimacranthoideⅤ（2） were isolated from the flower buds of Lonicera macranthoides Hand.-Mazz.（Caprifoliaceae）.They have hederagenin as aglycone.LonimacranthoideⅣ（1） is a rare chlorogenic acid ester acylated on the C-23 of hederagenin.LonimacranthoideⅣis a new sulfated triterpenoid saponin.The structures of the saponins were established by chemical and spectral methods.

广西产红腺忍冬与灰毡毛忍冬红外光谱初探

[目的]建立红腺忍冬与灰毡毛忍冬红外光谱快速鉴别的评价方法。[方法]采用红外光谱法对采自广西10个不同产地的红腺忍冬样品和6个不同产地的灰毡毛忍冬进行红外光谱试验研究,运用Omnic 7.0软件对红外图谱进行平滑机基线校正处理,并以他们的平均图谱为标准求出各样品的相似度,通过其红外光谱特征吸收峰及各样品的相似度对样品进行品种鉴别评价。[结果]得到广西产红腺忍冬和灰毡毛忍冬的特征吸收峰,并求得红腺忍冬各样品的相似度在0.993 4～0.976 7,相似度的RSD值为0.58%(n=10);灰毡毛忍冬各样品的相似度在0.685 4～0.823 8,相似度的RSD值为6.92%(n=6)。[结论]该方法简便,快速,稳定,可用于红腺忍冬与灰毡毛忍冬的鉴别。

灰毡毛忍冬新品种‘渝蕾一号’的组织培养研究

目的：建立灰毡毛忍冬新品种‘渝蕾一号’Lonicera macranthoides Hand.-Mazz.‘Yulei1’组培快繁体系,为实现其工厂化育苗提供理论依据。方法：以‘渝蕾一号’幼嫩茎段和叶片为外植体,以MS为基本培养基,在组织培养的不同阶段添加不同浓度的NAA、6-BA、TDZ、IBA等植物生长调节物质,对愈伤组织的诱导、增殖及分化,茎段腋芽诱导、增殖及生根炼苗进行系统研究。结果：外植体的最佳灭菌时间：茎段为2~3 min,叶片为1~2 min;愈伤组织诱导最佳培养基为MS＋2,4-D 1.0 mg/L＋6-BA 0.1 mg/L;腋芽诱导最佳培养基为MS＋6-BA 1.0 mg/L＋NAA0.5 mg/L;愈伤组织增殖最佳培养基为MS＋TDZ（0.2~1.0）mg/L＋NAA（0.02~0.05）mg/L;愈伤组织分化最佳培养基为MS＋6-BA（0.5~1.0）mg/L＋NAA（0.02~0.05）mg/L;芽增殖最佳培养基为MS＋6-BA 1.5 mg/L＋NAA0.5 mg/L;最佳生根培养为1/2MS＋IBA 1.0 mg/L＋NAA 0.2 mg/L。试管苗在珍珠岩和腐殖质（1∶1）基质中炼苗,成活率达75%以上。结论：筛选出了外植体适宜的消毒方法及愈伤组织诱导、增殖、分化及芽增殖、生根的适宜培养基,建立了‘渝蕾一号’组培快繁体系。

灰毡毛忍冬组培苗生根过程中内源生长调节剂的变化

以灰毡毛忍冬(Lonicera macranthoides Hand.-Mazz)组培苗为试验材料,用双抗体一步夹心酶联免疫法测定灰毡毛忍冬生根诱导过程(0、6、12、18、24、30 d)中生长素(IAA)、脱落酸(ABA)、赤霉素(GA3)、玉米素核苷(ZR)含量的变化。结果表明,ABA含量呈先上升后下降的趋势;IAA、GA3和ZR含量均呈先下降后上升的趋势。IAA、GA3含量和IAA/ABA比值与生根率呈显著或极显著正相关,ABA含量与生根率呈负相关,ZR含量与生根率相关性不显著。