Analysis of Volatile Constituents in Lonicera japonica Thunb. from Different Origins by GC-MS

[Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS analysis was performed with EI and quadruple mass analyzer. The volatile constituents in L. japonica Thunb. were identified by NIST02 and Wiley275 libraries, and their relative contents were determined with chromatographic peak area normalization method. [Result] According to GC-MS total ion-current chromatograms, 35 volatile constituents were identified in L. japonica Thunb. from Guangxi Zhuang Autonomous Region, mainly including methyl linolenate, n-hexadecanoic acid and ζ-muurolene; 18 volatile constituents were identified in L. japonica Thunb. from Hunan Province, mainly including n-hexadecanoic acid, linoleic acid and α-curcumene. [Conclusion] Main volatile constituents in L. japonica Thunb. from two different origins varied significantly.

Effect of Different Factors on Rooting Characteristics and Survival of Lonicera japonica Thunb

[Objective] The aim was to explore the effects of different factors on rapid propagation and seedling survival of Lonicera japonica Thunb. [Method] The cuttings of 1, 2 or 3 years old, with different length, were soaked in different hormones at different concentrations for different time and planted at different dates. Finally, their rooting rate and survival rate in different treatments were measured and compared. [Result] The rooting rate and survival rate of one-year old cuttings with length of 10-13 cm and planted on September 20 and October 20, 2012 were relatively higher among all the treatments. Hormone treatment could significantly promote the callus formation and accelerate the rooting of the cuttings, and improve their survival rate. The cuttings treated with 120-200 mg/L ABT1 or NAA for 35-45 min had higher rooting rate and survival rate. Among them, the survival rate of cuttings treated with 160 mg/L ABT1 for 35 min was the highest, up to 92.5%. [Conclusion] The study provided basis for improving the survival rate of L. japonica by cuttage, and for optimizing the rapid propagation technique.

HPLC测定“北花一号”金银花不同发育时期马钱苷的含量

为了测定“北花一号”金银花不同时期马钱苷的含量及确定最佳采收时期,采用高效液相色谱法(HPLC)测定其花朵4个发育时期马钱苷的含量。结果表明,以ZOR BAX Eclipse XDB-C18(250.0 mm×4.6 mm,5 μm)为色谱柱,乙腈-0.1%甲酸水为流动相,梯度洗脱35 min,流速1 mL/min,进样量10 μL,紫外检测波长为245 nm,金银花中马钱苷在5~200 μg/mL(R^(2)=0.999 8)范围内线性关系良好,精密度、稳定性、重复性、加标回收率均符合药典规定,建立准确、可靠、灵敏的HPLC测定马钱苷的方法。“北花一号”金银花中马钱苷的含量随着发育时期呈先上升后下降的趋势,三青期马钱苷的含量最高达0.103 2%,其次是二白期,大白期含量最低,考虑有效成分总含量可以选择二白期为最佳采收时期,为金银花中马钱苷含量测定及合理采收金银花提供理论依据。

Protective effect of different medicinal parts of Lonicera japonica Thunb. on myocardial ischemic injury induced by isoproterenol in rats

Background:To study the protective effect of different medicinal parts of honeysuckle on myocardial ischemia injury in rats.Method:SD mice were randomly divided into 6 groups:the normal group,model group,compound salvia miltiorrhiza group,Lonicera japonica Thunb.of flower bud group,flower group,and the stem leaf group.There were 8 rats in each group,including 4 males and 4 females.The normal group and model group were given equal amount of distilled water,whereas the other dosage groups were given the appropriate drug by intragastric administration,continuous gavage for 14 days.From the 12 days,in addition to the normal group were injected of the same amount of normal saline,the other groups were injected with 5g/kg.Injection of isoproterenol to establish the mouce mycardial ischemia model,its continuously 3d.To detect the serum of AST,CK,CK-MB,LDH and the myocardial issue of Chinese super oxideismutase and malondialdehyde level.Results:Compared with normal group,the levels of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde were significantly increased in the model group(P<0.01)and the level of super oxideismutase decreased significantly(P<0.01)the levels of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde in the serum of flower bud group,flower group and stem leaf group were significantly decreased(P<0.05),and the decreasing level of flower bud group,flower,leaf group of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde was flower bud group>flower>leaf group,the activity of super oxideismutase was obviously increased,and the activity level of super oxideismutase of flower bud group,flower group and stem leaf group was flower bud group>flower>leaf group.Conclusion:In the myocardial ischemic injury model of rats induced by isoproterenol,Lonicera japonica Thunb.had a protective effect in all parts,and Lonicera japonica Thunb.bud had the greatest protective effect.

金银花根际土壤放线菌分离及其抑菌活性测定

为研究金银花(Lonicera japonica Thunb.)根际土壤放线菌资源及其抗菌活性,采用稀释涂布法对金银花根际土壤的放线菌进行分离纯化,共得到7株不同形态特征的放线菌。通过滤纸片法进行抑菌活性探究,筛选出2株放线菌对金黄色葡萄球菌和枯草芽孢杆菌有一定的抑制作用,编号为4和7。经分子鉴定,4号、7号放线菌均属于链霉菌属,分别为黄三素链霉菌(Streptomyces flavotricin)和加纳链霉菌(Streptomyces gardneri)。

Extraction Process and Content Determination of Total Flavonoids in Lonicera japonica

[Objectives]To optimize the extraction process of total flavonoids of Lonicerae japonica and establish a method for its extraction and content determination.[Methods]The total flavonoids of L.japonica were extracted by reflux extraction method.Through a single factor experiment,the effects of extraction method,extraction solvent concentration,extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of L.japonica.[Results]The optimal extraction process of the total flavonoids of L.japonica was 70%ethanol,1∶30 of solid-to-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]This method can effectively determine the content of total flavonoids in L.japonica and is expected to provide a certain scientific basis for the study of the quality standard of L.japonica.This method has high reproducibility.It is stable and feasible in extraction of total flavonoids from L.japonica.

金银花绿原酸基因的发掘及生物信息学分析

绿原酸(chlorogenic acid, CGA)是金银花(Lonicera japonica Thunb.)重要的功效成分,具有抗菌、抗病毒、防癌、防衰老等作用。本研究参照17个物种中33个绿原酸基因编码蛋白的氨基酸序列,通过NCBIBLAST扫描金银花蛋白组数据库,结合亲缘关系分析和结构域分析,筛选获得5个调控金银花绿原酸候选基因,均为Phenylalanine amononia-lyase,分别命名为LjPAL1、LjPAL2、LjPAL3、LjPAL4、LjPAL5。理化性质分析和亚细胞定位发现,这5个候选基因编码的氨基酸个数为686~714,理论等电点为5.85~6.26,均为稳定的亲水性蛋白,均定位在细胞质中。本研究为金银花的分子标记辅助育种提供基因资源,为金银花绿原酸合成代谢途径的研究提供科学依据。

树型金银花外植体诱导技术

以树型金银花(Lonicera japonica Thunb.)萌芽条为外植体,开展不同外植体部位、消毒剂组合及诱导培养基、不同季节对无菌芽诱导的研究,筛选外植体最佳诱导方式,建立树型金银花组织培养无菌体系。结果表明:外植体选择顶芽以下第2个芽节至第4个芽节,先用75%酒精消毒30s,用0.1%升汞消毒10min,同时加1滴吐温-80的诱导方式成活率最高,达70.33%,最佳诱导培养基为MS附加6-BA 1.5mg/L、NAA 0.1mg/L,诱导萌芽率为92.33%,最适合诱导的季节是秋季,成功率为78.33%。