期刊文献+
共找到16篇文章
< 1 >
每页显示 20 50 100
Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences 被引量:1
1
作者 孟庆磊 王师 +2 位作者 张玲玲 黄晓婷 包振民 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2013年第1期128-133,共6页
In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP... In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP is a method that is generally more specific and sensitive than conventional techniques such as fluorescence in situ hybridization (FISH), primed in situ labeling (PRINS), and cycling primed in situ labeling (C-PRINS). Here, we describe the development and application of in situ LAMP to identify the chromosomal localization of DNA sequences. To benchmark this technique, we successfully applied this technique to localize the major ribosomal RNA gene on the chromosomes of the Zhikong scallop ( Chlarnys farreri). 展开更多
关键词 chromosomal localization in situ loop-mediated isothermal amplification (in situ lamp major rRNA Chlamys farreri
下载PDF
The Development of a Loop-Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic 被引量:1
2
作者 Mariana de Lira Nunes Carina Lucena Mendes-Marques +1 位作者 Alzira Maria Paiva de Almeida Nilma Cintra Leal 《American Journal of Analytical Chemistry》 2014年第16期1069-1077,共9页
Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control m... Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65&deg;C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies. 展开更多
关键词 PLAGUE YERSINIA PESTIS Diagnosis Tests loop-mediated isothermal amplification (lamp)
下载PDF
Preliminary investigation and detection based on loop-mediated isothermal amplification(LAMP)of phytoplasmas associated with diseases in B.napus L.
3
作者 Yancheng Wen Shufen Zhang +8 位作者 Junping He Dongfang Cai Jiacheng Zhu Jianping Wang Jinhua Cao Kun Hu Lei Zhao Dongguo Wang Yizi Liu 《Oil Crop Science》 CSCD 2022年第4期219-224,共6页
In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detectio... In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L. 展开更多
关键词 B.napus L. Phytoplasma associated disease PATHOGEN DETECTION loop-mediated isothermal amplification (lamp)
下载PDF
One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA 被引量:9
4
作者 Xue-en FANG Jian LI Qin CHEN 《Virologica Sinica》 SCIE CAS CSCD 2008年第3期167-172,共6页
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of fo... Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article. 展开更多
关键词 Nucleic acid amplification loop-mediated isothermal amplification lamp APPLICATION
下载PDF
Development of a Loop-Mediated Isothermal Amplification Assay for Porcine Circovirus Type 2 被引量:3
5
作者 Ye-bing Liu Lei Zhang +2 位作者 Qin-hong Xue Yi-bao Ning Zhi-gang Zhang 《Virologica Sinica》 SCIE CAS CSCD 2011年第3期214-220,共7页
In this study, the loop-mediated isothermal amplification (LAMP) method was used to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). According to the PCV2 sequences published in GenBan... In this study, the loop-mediated isothermal amplification (LAMP) method was used to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). According to the PCV2 sequences published in GenBank, multiple LAMP primers were designed targeting conserved sequences of PCV2. Using the DNA extracted from PCV2 isolates HUN-09 and SD-09 as the template, LAMP reactions in a PCV2 LAMP system was performed, the amplification products were detected by adding SYBR Green I and could be observed directly by the naked eye. The results showed highly-efficient and specific amplification in 30 min at 63°C with a LAMP real-time turbidimeter. Furthermore, PCV2 DNA templates, with a detection limit of 5.5×10-5 ng of nucleic acid, indicated that this assay was highly sensitive. The results obtained with the naked eye after SYBR Green I staining were consistent with those detected by the real-time turbidimeter, showing the potential simplicity of interpretation of the assay results. The LAMP assay appeared to have greater accuracy than PCR and virus isolation for the analysis of 18 clinical samples. In addition it offers higher specificity and sensitivity, shorter reaction times and simpler procedures than the currently available methods of PCV2 detection. It is therefore a promising tool for the effective and efficient detection of PCV2. 展开更多
关键词 Porcine circovirus type 2 (PCV2) loop-mediated isothermal amplification lamp Virus detection
下载PDF
Development and Evaluation of a Loop-mediated Isothermal Amplification Assay for the Rapid Detection and Identification of Pectobacterium carotovorum on Celery in the Field 被引量:3
6
作者 Yanxia Shi Zhiwen Jin +4 位作者 Xianglong Meng Lixue Wang Xuewen Xie Ali Chai Baoju Li 《Horticultural Plant Journal》 SCIE 2020年第5期313-320,共8页
Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species.Once P.carotovorum infects the plant,the spread of the disease is difficult to control.In this study,a rap... Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species.Once P.carotovorum infects the plant,the spread of the disease is difficult to control.In this study,a rapid and sensitive method based on loop-mediated isothermal amplification(LAMP)was developed for detecting P.carotovorum in celery with soft rot using a primer set designed from the pmrA conserved sequence of P.carotovorum.The specificity of the LAMP primer set for P.carotovorum was extensively validated on both P.carotovorum strains and nontarget strains.The sensitivity was 1 pg of P.carotovorum genomic DNA,which demonstrated 10 times more sensitive than the conventional PCR assay.LAMP was also used to detect P.carotovorum in bacterial suspension.The lowest detection concentration was 104 CFU·mL^−1.In addition,a LAMP assay,in conjunction with a crude DNA extraction method,was successfully performed on P.carotovorum-infected samples derived from both artificially and naturally infected plants.In summary,the LAMP assay established in this study constitutes a simple,sensitive,and rapid method for the detection of P.carotovorum,and has potential application in the control of celery soft rot disease through early detection. 展开更多
关键词 Bacterial soft rot P.carotovorum loop-mediated isothermal amplification(lamp) CELERY pmrA gene field detection
下载PDF
Rapid,sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification 被引量:2
7
作者 高宏伟 李富花 +2 位作者 张晓军 王兵 相建海 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第1期62-66,共5页
Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine... Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65℃ in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V, anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field. 展开更多
关键词 loop-mediated isothermal amplification lamp detection assay empA gene Vibrio anguillarum
下载PDF
Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification 被引量:1
8
作者 ZHANG Fengying SHI Yanhong +2 位作者 JIANG Keji XU Zhaoli MA Lingbo 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第2期139-146,共8页
A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid de- tection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (... A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid de- tection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection lim- its of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum. 展开更多
关键词 Alexandrium eatenella Alexandrium minutum detection loop-mediated isothermal amplification lamp ribosomal DNA internal transcribed spacer (ITS)
下载PDF
Combination of Loop-Mediated Isothermal Amplification Assay and Nested PCR for Detection of Borrelia burgdorferi sensu lato in Human Serum Samples 被引量:1
9
作者 ZHANG Liu Li HOU Xue Xia +3 位作者 GENG Zhen LOU Yong Liang WAN Kang Lin HAO Qin 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第4期312-315,共4页
A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAM... A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.I. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.I. in human serum samples. 展开更多
关键词 PCR lamp Combination of loop-mediated isothermal amplification Assay and Nested PCR for Detection of Borrelia burgdorferi sensu lato in Human Serum Samples
下载PDF
新疆外来入侵杂草豚草属LAMP快速检测技术的建立
10
作者 于海鑫 付开赟 +4 位作者 丁新华 贾尊尊 吐尔逊·阿合买提 王兰 郭文超 《生物安全学报》 CSCD 北大核心 2023年第3期277-281,共5页
【目的】开发外来入侵生物三裂叶豚草和豚草不同生育期、不同部位的环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术,以达到田间快速、准确和高效识别的目的。【方法】以SYBR Green Ⅰ为指示剂,分别针对三裂叶豚草和... 【目的】开发外来入侵生物三裂叶豚草和豚草不同生育期、不同部位的环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术,以达到田间快速、准确和高效识别的目的。【方法】以SYBR Green Ⅰ为指示剂,分别针对三裂叶豚草和豚草不同发育阶段(幼苗期、生长期、种子期)开展LAMP技术开发。【结果】特异性验证结果显示,所检测杂草的LAMP产物均呈阳性(产生白色沉淀),而与其对照的其他2种杂草的LAMP产物均为阴性(无白色沉淀)。灵敏度检测结果显示,该体系的DNA最低检测限为10^(-10) ng·μL-1,比常规聚合酶链式反应灵敏度高。【结论】本研究建立的LAMP检测体系能有效应用于三裂叶豚草和豚草样本的快速检测,为其快速、高效识别提供技术支撑。 展开更多
关键词 三裂叶豚草 豚草 环介导等温扩增(lamp) 快速检测技术
下载PDF
桑源阴沟肠杆菌LAMP检测方法的建立和应用 被引量:4
11
作者 杨宏宇 周轶楠 +8 位作者 孙勋勋 王亚洁 王狗旦 陈杰湖 易辉玉 刘伟强 黄志君 田铃 刘吉平 《蚕业科学》 CAS CSCD 北大核心 2019年第3期321-330,共10页
桑源阴沟肠杆菌是引起桑树发生枯萎症状的重要病原之一。对桑源阴沟肠杆菌全基因组序列进行分析,得到桑源阴沟肠杆菌特异基因序列,并以该特异基因序列为靶序列,设计筛选合适的环介导等温扩增(loop-mediated isothermal amplification,LA... 桑源阴沟肠杆菌是引起桑树发生枯萎症状的重要病原之一。对桑源阴沟肠杆菌全基因组序列进行分析,得到桑源阴沟肠杆菌特异基因序列,并以该特异基因序列为靶序列,设计筛选合适的环介导等温扩增(loop-mediated isothermal amplification,LAMP)引物组,通过荧光扩增曲线法、PCR产物琼脂糖凝胶电泳法及PCR产物SYBR Green Ⅰ染色法3种判定方法优化反应体系,进而建立桑源阴沟肠杆菌LAMP检测技术和产品。采用Yt4引物组,在60℃、外内引物浓度比为1∶10的最优反应条件下,桑源阴沟肠杆菌阳性质粒检测灵敏度为25×10^6拷贝/μL。在特异性实验中,桑源阴沟肠杆菌菌株的阳性反应能产生S型荧光扩增曲线,电泳法可得到梯形条带,染色法反应液变为绿色,而在其他供试菌株及阴性对照中均未发生这些现象。应用建立的LAMP检测法和普通PCR方法分别对来自4个不同地区的11个桑树枯萎症状样品进行同步平行检测,2种方法的检测结果一致,但LAMP检测法更简单、快捷和灵敏。建立的桑源阴沟肠杆菌LAMP检测方法,为桑源阴沟肠杆菌的快速检测及桑树枯萎病的诊断提供了新技术。 展开更多
关键词 桑源阴沟肠杆菌 环介导等温扩增技术 检测
下载PDF
猪圆环病毒1型LAMP检测方法的建立及初步应用 被引量:6
12
作者 田瑶 李佳昕 +9 位作者 杨莹 王硕 时建立 彭喆 李琛 徐绍建 吴晓燕 刘畅 韩红 李俊 《山东农业科学》 北大核心 2021年第3期127-131,共5页
为建立猪圆环病毒1型(PCV1)环介导等温扩增(LAMP)检测方法,为现场诊断提供技术支持,本试验根据GenBank公布的PCV1全基因组序列,在其保守区设计了4条特异性LAMP引物,并通过反应条件优化、敏感性试验、特异性试验以及临床样本检测,首次建... 为建立猪圆环病毒1型(PCV1)环介导等温扩增(LAMP)检测方法,为现场诊断提供技术支持,本试验根据GenBank公布的PCV1全基因组序列,在其保守区设计了4条特异性LAMP引物,并通过反应条件优化、敏感性试验、特异性试验以及临床样本检测,首次建立了PCV1的LAMP检测方法。结果表明,62℃水浴60min为最佳反应条件;LAMP的检出限为1×10^(1)copies/μL,普通PCR最低检测限为1×10^(3)copies/μL;对猪细小病毒(PPV)、猪伪狂犬病毒(PRV)、猪圆环病毒2型(PCV2)、猪圆环病毒3型(PCV3)进行扩增,结果显示为阴性;应用该方法对103个临床疑似病料进行检测,阳性率为34.0%(35例),普通PCR阳性率为29.1%(30例),两种检测方法符合率为89.5%。本试验为PCV1临床检测提供了一种特异、快速、成本低廉的方法。 展开更多
关键词 猪圆环病毒1型 环介导等温扩增技术 特异性 敏感性
下载PDF
不同方法检测子宫内膜炎患者解脲支原体和生殖支原体感染的实验研究 被引量:4
13
作者 高丹 向华国 《海南医学》 CAS 2018年第16期2278-2280,共3页
目的探究环介导恒温扩增技术(LAMP)、实时恒温扩增RNA技术(SAT)和连接酶链反应法(LCR)对子宫内膜炎患者解脲支原体(UU)和生殖支原体(MG)检出率的差异。方法收集2015年12月至2017年4月就诊于深圳市宝安区福永人民医院的子宫内膜炎患者186... 目的探究环介导恒温扩增技术(LAMP)、实时恒温扩增RNA技术(SAT)和连接酶链反应法(LCR)对子宫内膜炎患者解脲支原体(UU)和生殖支原体(MG)检出率的差异。方法收集2015年12月至2017年4月就诊于深圳市宝安区福永人民医院的子宫内膜炎患者186例,分别采用三种方法对患者宫颈分泌物进行检测,对测量结果进行特异性和敏感度比较。结果 SAT检测UU的敏感度为91.5%,与LAMP、LCR检测方法(88.4%和83.1%)比较,差异均有统计学意义(P<0.05);SAT与LAMP对MG的敏感度分别为86.8%和86.0%,差异无统计学意义(P>0.05),但是均明显高于LCR的79.2%,差异均有统计学意义(P<0.05);SAT方法诊断UU合并MG的敏感度最高,为83.3%,与LCR方法的75.5%比较差异有统计学意义(P<0.05),SAT和LAMP检测的特异度分别为96.8%、96.6%,差异无统计学意义(P>0.05)。结论相对于LAMP和LCR,SAT对子宫内膜炎患者UU和MG的检测具有较好的敏感性,该方法的独特优势值得在女性专科和大型综合医院实验室推荐使用。 展开更多
关键词 环介导恒温扩增技术 实时恒温扩增RNA技术 连接酶链反应法 解脲支原体 生殖支原体
下载PDF
植物病原真菌分子检测技术的研究进展 被引量:23
14
作者 彭丹丹 张源明 +1 位作者 舒灿伟 周而勋 《基因组学与应用生物学》 CAS CSCD 北大核心 2017年第5期2015-2022,共8页
真菌是一类非常重要的植物病原菌。对这类病原菌进行分子快速检测是病害早期诊断、检疫、监测、预测预报和病害防治等工作的重要基础。本综述详细介绍与评述了一些常见的以PCR技术为基础的检测技术以及近年来发展起来的一些新技术如环... 真菌是一类非常重要的植物病原菌。对这类病原菌进行分子快速检测是病害早期诊断、检疫、监测、预测预报和病害防治等工作的重要基础。本综述详细介绍与评述了一些常见的以PCR技术为基础的检测技术以及近年来发展起来的一些新技术如环介导等温扩增和核酸滚环扩增技术在植物病原真菌分子检测中的应用,旨在对该研究领域的进展有一个全面的了解。 展开更多
关键词 植物病原真菌 分子检测 基于PCR的技术 环介导等温扩增 核酸滚环扩增
原文传递
环介导等温扩增反应的原理及应用 被引量:6
15
作者 黄思佳 解庭波 严家新 《中国生物制品学杂志》 CAS CSCD 2011年第12期1511-1513,共3页
环介导等温扩增反应(Loop-mediated isothermal amplification,LAMP)是日本学者Notomi于2000年在NucleicAcids Res杂志上公开的一种新的基因诊断技术,该法操作简便,扩增效率及灵敏度高,已广泛应用于食品、临床、农业等领域,有望成为主... 环介导等温扩增反应(Loop-mediated isothermal amplification,LAMP)是日本学者Notomi于2000年在NucleicAcids Res杂志上公开的一种新的基因诊断技术,该法操作简便,扩增效率及灵敏度高,已广泛应用于食品、临床、农业等领域,有望成为主流的基因诊断方法。本文对LAMP的反应特征及原理、优缺点、临床应用作一综述。 展开更多
关键词 环介导等温扩增反应 核酸扩增技术
原文传递
Charting the challenges behind the testing of COVID-19 in developing countries:Nepal as a case study 被引量:2
16
作者 Anil K.Giri Divya RSJB Rana 《Biosafety and Health》 2020年第2期53-56,共4页
The infrastructure needed to detect Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)infection(COVID-19)that complies completely withWHO guidelines is lacking across many parts of the globe,especially in dev... The infrastructure needed to detect Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)infection(COVID-19)that complies completely withWHO guidelines is lacking across many parts of the globe,especially in developing countries,including Nepal.We outline the problems faced by such countries and suggest that the national and international community should collaborate in the development and adoption of novel protocols for the rapid detection of COVID-19 according to locally available infrastructure,in order to fight against the outbreak. 展开更多
关键词 COVID-19 SARS-CoV-2 loop-mediated isothermal amplification(lamp) ASSAY Biosafety Level-2(BSL-2)
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部