[Objectives]This study aimed to optimize the extraction process of longan(Dimocarpus longan Lour.)leaves by multi-indicator comprehensive evaluation method.[Methods]The contents of gallic acid ethyl ester,astragalin,q...[Objectives]This study aimed to optimize the extraction process of longan(Dimocarpus longan Lour.)leaves by multi-indicator comprehensive evaluation method.[Methods]The contents of gallic acid ethyl ester,astragalin,quercetin,luteolin and kaempferol in longan leaves were determined simultaneously by HPLC.Using the multi-indicator comprehensive evaluation method,the methanol concentration,solid to liquid ratio and extraction time for extraction of longan leaves were optimized by orthogonal test.[Results]The optimal extraction process of longan leaves are as follows:methanol concentration of 100%,solid to liquid ratio of 1∶5,and extraction time of 50 min.[Conclusions]The optimized process is simple and feasible,and it can be used to determine the contents of different ingredients in longan leaves.展开更多
基金National Natural Science Foundation of China(81560691)Special Fund Project for Innovation Driven Development of Guangxi(Gui Ke AA17202046)+7 种基金Open Program for Construction of TCM Pharmacy Doctor Station in Guangxi University of Chinese Medicine(201410-06)The First-Class Subject of Traditional Chinese Medicine(Ethnic Pharmacy)in Guangxi(Gui Jiao Ke Yan[2018]No.12)Program of Collaborative Innovation Center of Zhuang and Yao Medicine(Gui Jiao Ke Yan[2013]No.20)Program of Key Laboratory of Zhuang and Yao Medicine[Gui Ke Ji Zi[2014]No.32]The Eighth Batch of Special Experts Project of Guangxi Zhuang Autonomous Region(Study on Quality Standard of Zhuang&Yao Medicine,[2019]No.13)High-level Talent Team Cultivation Program of"Qihuang Project"of Guangxi University of Chinese Medicine(2018002)High Level Innovation Team and Excellent Scholar Program of Guangxi Universities(Gui Jiao Shi Fan[2019]No.52)Guangxi One Thousand Young and Middle-aged College and University Backbone Teachers Cultivation Program(Gui Jiao Ren[2019]No.5).
文摘[Objectives]This study aimed to optimize the extraction process of longan(Dimocarpus longan Lour.)leaves by multi-indicator comprehensive evaluation method.[Methods]The contents of gallic acid ethyl ester,astragalin,quercetin,luteolin and kaempferol in longan leaves were determined simultaneously by HPLC.Using the multi-indicator comprehensive evaluation method,the methanol concentration,solid to liquid ratio and extraction time for extraction of longan leaves were optimized by orthogonal test.[Results]The optimal extraction process of longan leaves are as follows:methanol concentration of 100%,solid to liquid ratio of 1∶5,and extraction time of 50 min.[Conclusions]The optimized process is simple and feasible,and it can be used to determine the contents of different ingredients in longan leaves.