基于LotusDomino的OA系统开发与实现

OA系统的建设,是促进企业信息化建设、实现企业快速发展的重要保障。通过从某企业的实际需求出发,对OA系统从C/S结构向B/S结构转换,以Lotus Domino作为开发平台,详细阐述了设计开发B/S办公自动化系统的流程和关键要素,并介绍了进行系统测试的常用方法。

Co_(3)S_(4)-pyrolysis lotus fiber flexible textile as a hybrid electrocatalyst for overall water splitting

Electrocatalytic overall water splitting(OWS),a pivotal approach in addressing the global energy crisis,aims to produce hydrogen and oxygen.However,most of the catalysts in powder form are adhesively bounding to the electrodes,resulting in catalyst detachment by bubble generation and other uncertain interference,and eventually reducing the OWS performance.To surmount this challenge,we synthesized a hybrid material of Co_(3)S_(4)-pyrolysis lotus fiber(labeled as Co_(3)S_(4)-p LF)textile by hydrothermal and hightemperature pyrolysis processes for electrocatalytic OWS.Owing to the natural LF textile exposing the uniformly distributed functional groups(AOH,ANH_(2),etc.)to anchor Co_(3)S_(4)nanoparticles with hierarchical porous structure and outstanding hydrophily,the hybrid Co_(3)S_(4)-p LF catalyst shows low overpotentials at 10 m A cm^(-2)(η_(10,HER)=100 m Vη_(10,OER)=240 mV)alongside prolonged operational stability during electrocatalytic reactions.Theoretical calculations reveal that the electron transfer from p LF to Co_(3)S_(4)in the hybrid Co_(3)S_(4)-p LF is beneficial to the electrocatalytic process.This work will shed light on the development of nature-inspired carbon-based materials in hybrid electrocatalysts for OWS.

Predicting bioactive compounds and cancer-related molecular targets of lotus seedpod (Receptaculum Nelumbinis) based on network pharmacology and molecular docking

Background:Lotus seedpod(Receptaculum Nelumbinis)is the abundant by-products produced during lotus seed processing,and the sources are usually considered to be wastes and are abandoned outdoors or incinerated.This study aims at predicting its bioactive compounds and cancer-related molecular targets against six cancers,including lung cancer,gastric cancer,liver cancer,breast cancer,ovarian cancer and cervical cancer.Methods:Network pharmacology and molecular docking methods were performed.Results:Network pharmacology results indicated that 14 core compounds(liensinine,tetrandrine,lysicamine,tricin,sanleng acid,cireneol G,ricinoleic acid,linolenic acid,5,7-dihydroxycoumarin,apigenin,luteolin,morin,quercetin and isorhamnetin)and 10 core targets(AKT1,ESR1,HSP90AA1,JUN,MAPK1,MAPK3,PIK3CA,PIK3R1,SRC and STAT3)were screened for lotus seedpod against the six cancers.Molecular docking analysis suggested that the binding abilities between the core compounds and the core targets were mostly strong.GO analysis revealed that the intersected targets between the bioactive compounds of lotus seedpod and the six cancers were significantly related to biological processes,cell compositions and molecular functions.KEGG analysis showed that PI3K-Akt,TNF,Ras,MAPK,HIF-1 and C-type lectin receptor signaling pathways were notably involved in the anti-cancer activities of lotus seedpod against the six cancers.Conclusions:14 core compounds and 10 core targets were screened for lotus seedpod against lung cancer,gastric cancer,liver cancer,breast cancer,ovarian cancer and cervical cancer.This study supports the application of lotus seedpod in treating cancers,and promotes the recycling and the high-value utilization.

Quercetin-derivatives painting the yellow petals of American lotus(Nelumbo lutea)and enzymatic basis for their accumulation

American lotus(Nelumbo lutea)is one of the two species in Nelumbo and has only yellow flower.Identification of total flavonoids showed wild American lotus contained almost only flavonols with quercetin 3-O-glucuronide to be the dominant pigment.The variation tendency of the total flavonol content was coincident with yellow color variation of petals during flower development.To understand the mechanism of accumulation and constituent of pigments in petals,three pivotal genes,NlFLS1,NlFLS2 and NlFLS3,which were predicted to encode flavonol synthases were isolated and characterized by analyses of basic bioinformatics,temporal and spatial expression patterns and enzymatic activity.Their temporal expression levels showed the same variation tendency,which was also consistent with the development-dependent variation of total flavonol content.Spatial expression patterns indicated the three genes should function in petals.All the three proteins were demonstrated to be bifunctional dioxygenase,possessing both flavonol synthase activity and flavanone 3-hydroxylase activity.Besides,other flavonol biosynthesis related genes were also investigated on their expression levels to give more clues on the mechanism.Substrate preferences of the three FLSs,substrate competitions between the FLSs and other flavonol biosynthesis related enzymes,and the greatly differential expression levels between F3’H(flavonoid 3-hydroxylase)and F3’5’H(flavonoid 3,5-hydroxylase)contributed to the flavonol constituent in the petals of America lotus,namely abundant quercetin-derivatives while very few kaempferol-derivatives and myricetin-derivatives.

Genetic diversity and inferred ancestry of Asian lotus(Nelumbo nucifera) germplasms in Thailand and Vietnam

Tropical lotus(Nelumbo) is an important and unique ecological type of lotus germplasm.Understanding the genetic relationship and diversity of the tropical lotus is necessary for its sustainable conservation and utilization.Using 42 EST-SSR(expressed sequence tag-simple sequence repeats) and 30 SRAP(sequence-related amplified polymorphism) markers,we assessed the genetic diversity and inferred the ancestry of representative tropical lotus from Thailand and Vietnam.In total,164 and 41 polymorphic bands were detected in 69 accessions by 36 EST-SSR and seven SRAP makers,respectively.Higher genetic diversity was revealed in Thai lotus than in Vietnamese lotus.A Neighbor-Joining tree of five main clusters was constructed using combined EST-SSR and SRAP markers.Cluster Ⅰ included 17 accessions of Thai lotus;cluster Ⅱ contained three Thai accessions and 11 accessions from southern Vietnam;and cluster Ⅲ was constituted by 13 accessions of seed lotus.Consistent with the results from the NeighborJoining tree,the genetic structure analysis showed that the genetic background of most Thai and Vietnamese lotus was pure,as artificial breeding has been rare in both countries.Furthermore,these analyses indicate that Thai and Vietnamese lotus germplasms belong to two different gene pools or populations.Most lotus accessions are genetically related to geographical distribution patterns in Thailand or Vietnam.Our findings showed that the origin or genetic relationships of some unidentified lotus sources can be evaluated by comparing morphological characteristics and the data of molecular markers.In addition,these findings provide reliable information for the targeted conservation of tropical lotus and parent selection in breeding novel cultivars of lotus.

The high-quality genome of lotus reveals tandem duplicate genes involved in stress response and secondary metabolites biosynthesis

Dear Editor,Lotus(Nelumbo nucifera Gaertn.),which has been domesticated and cultivated for several thousands of years and endowed with religious and cultural symbolism[1],belongs to the Nelumbo genus Nelumbonaceae family.As an early eudicot,it is not only essential for plant phylogeny but also widely used as a vegetable,a medic-inal herb,and for ornamental use.It contains abundant func-tional compounds,such as f lavonoids and benzylisoquinoline alkaloids(BIAs),which are used to treat diverse diseases.

Optimization of slow-release fertilizer application improves lotus rhizome quality by affecting the physicochemical properties of starch

To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.However,the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.This study was designed to investigate the photosynthetic characteristics and the synthesis,accumulation,and physicochemical properties of lotus rhizome starches under six SRF levels(CK,S1,S2,S3,S4,and S5).Compared with CK(0 kg ha^(–1)),the net photosynthetic rate(P_(n))and SPAD values of leaves remained at higher levels under SRF treatment.Further research showed that SRF increased the lotus rhizome yield,the contents of amylose,amylopectin,and total starch,and the number of starch granules.Among the six SRF levels,S3(1035 kg ha^(–1))showed the greatest difference from CK and produced the highest levels.With the increasing SRF levels,the peak,hot and final viscosities decreased at first and then increased,but the setback viscosity and pasting temperature increased.In order to interpret these changes at the molecular level,the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.Each of these parameters also increased under SRF treatment,especially under the S3 treatment.The results of this study show that SRF,especially S3(1035 kg ha^(–1)),is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.

Lotus root polysaccharide inhibits the growth of ovarian cancer cells by blocking the cell cycle

Background:Lotus root polysaccharide is a natural antioxidant.As a new anticancer drug,it has anti-proliferation and pro-apoptotic effects in a variety of tumour cells,but its effect on ovarian cancer is not clear.In study,we attempted to elucidate the role and mechanism of lotus root polysaccharide in SKOV3 cells.Methods:In this study,the effect of lotus root polysaccharide on mRNA of SKVO3 cells was analyzed by RNA-seq,and verified by Western blot,flow cytometry,fluorescence detection and other techniques.Results:The results showed that lotus root polysaccharide could inhibit the proliferation of ovarian cancer cells.Then,a change in gene expression was found by RNA-seq.In the mRNA(differentially expressed mRNA)with these differences,significant changes in the cell cycle were found by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.Subsequently,the proportion of cells in S phase decreases and G2/M phase increases,as seen with propidium iodide staining.Gene Set Enrichment Analysis showed inhibition of the cell cycle,and the gene and protein expression of CDK1,CCNA1 and CCNB1 were inhibited.Conclusion:Our results show that lotus root polysaccharide can inhibit the growth of SKOV3 cells in vitro by blocking the cell cycle at the G2/M phase,which reveals the potential of lotus root polysaccharide in the treatment of ovarian cancer.

Ecological Risk Assessment of Heavy Metals Pollution in Soil and Lotus Seeds in Xiangtan Lotus Planting Area

In order to study the pollution characteristics and potential ecological risks of heavy metals in soil of Xiangtan lotus planting area,the contents of Mn,Cu,As,Cd,Pb and Cr in soil and lotus seeds were analyzed in six main lotus planting areas.The comprehensive potential ecological risks of heavy metals in soil were evaluated by the potential ecological hazard index.The results showed that Cd concentration(mean value)was higher than the risk screening value and lower than the risk control value,and other heavy metals were lower or close to the risk screening value.The ecological risks of each element from large to small were Cd,Pb,As,Cu,Cr and Mn.The average value of the comprehensive ecological hazard index of the six sites was 224.7,which was a medium level of pollution risk.The lotus seeds corresponding to each site were also polluted by heavy metals to a certain extent,and Cd and Pb in the seeds exceeded the standard.Through the above analysis,the heavy metals in the soil of the lotus planting area presented a medium ecological risk.In order to ensure the safety of lotus production,it is necessary to strengthen the soil testing and management in the lotus planting area.

Analysis on the Problems and Strategies of Marketing Channels of Agricultural Products : Taking Liujun Lotus Root as an Example

Marketing channels are an important part of the circulation of agricultural products.With the development of China s agricultural industry,the marketing channels of agricultural products are continuously innovated and optimized.Taking Liujun Lotus Root as an example,on the basis of studying the existing model of marketing channels of agricultural products,this paper analyzes the problems in the existing marketing channels of Liujun Lotus Root,and puts forward relevant strategies.

藕片热泵干燥工艺优化及全藕粉品质评价

为解决新鲜莲藕采后易霉变、品质下降等问题,以莲藕为原料,以藕片干燥速率、复水性,藕粉色泽、水分含量为指标,研究切片厚度、干燥温度和装载量对干燥后莲藕和全藕粉品质的影响。结果表明,当干燥温度70℃、切片厚度5 mm、装载量3 kg/m^(2)时,藕片干燥速率较快、复水性较好,全藕粉色泽鲜亮,含水量较低,为莲藕热泵干燥的最优条件。试验将新鲜莲藕加工成全藕粉,可有效保留莲藕的营养成分,为莲藕采后贮藏和加工提供理论依据。

美洲莲地下茎顶芽离体培养

以美洲莲地下茎顶芽为外植体,主要对消毒处理方法、顶芽初代培养激素种类及其浓度进行摸索,并比较取材时间和培养温度对顶芽离体培养成苗的影响。研究表明:对顶芽采取消毒后去掉一层鳞片再消毒,或在此基础上再去掉一层鳞片,将去掉一层或两层鳞片后的剩余部分接种至含有0.1%植物组培抗菌保护剂(Plant preservative mixture,PPM)或250 mg∕L头孢霉素的培养基中培养,污染率能降低到10.71%—15.99%。外植体在含3 mg∕L激动素(Kinetin,KT)+0.2 mg∕L萘乙酸(1-Naphthaleneacetic acid,NAA)的MS(Murashige and Skoog)培养基上培养1个月后转移至含1 mg∕L KT+0.1 mg∕L NAA的培养基上,或始终在含2 mg∕L KT+0.2 mg∕L NAA培养基上培养,能获得较好的成苗和增殖效果。10—12月份冬季休眠期的芽比3—5月份春季萌芽期至营养生长前期的芽更易培养成苗。30℃较25℃培养更利于顶芽的成苗。该研究建立的美洲莲地下茎顶芽离体培养体系可为该物种的推广应用以及试验研究提供一致性的材料和技术支持。

压热及酶解脱支处理对莲藕淀粉品质的影响

淀粉是莲藕的主要成分。本研究通过压热处理、酶解脱支处理进行莲藕淀粉改性,并对其品质进行比较。结果发现:改性后莲藕淀粉中直链淀粉与碘结合能力显著增强,其中普鲁兰酶脱支处理莲藕淀粉(ELRS)的碘结合力最高,即可溶性直链淀粉含量最高;水合能力显著增强,其中压热法处理莲藕淀粉(HLRS)的持水性、持油性和膨胀度(21.766%)最高,析水率(14.300%)最低;压热后普鲁兰酶脱支处理莲藕淀粉(HELRS)的溶解度(63.555%)、透明度(0.717%)最高;改性后莲藕淀粉的糊化起始温度(T0)、热焓值(ΔH)、糊化温度(ΔT)均有所下降,其中HELRS的ΔH最低,表明改性后熔解莲藕淀粉所需的能量减少;检测改性后淀粉的凝胶特性表明,莲藕淀粉的硬度、弹性、内聚性、粘性和回弹性均有所下降。该研究结论为食品生产中针对不同需求进行淀粉改性提供了理论依据,并为莲藕淀粉的高附加值产品开发提供指导。

嗜热酸性Ⅲ型普鲁兰水解酶协同压热制备莲子抗性淀粉的工艺优化及其功能特性研究

本研究以莲子淀粉为原料,采用嗜热酸性Ⅲ型普鲁兰水解酶(TK-PUL)与压热联合制备RS3型抗性淀粉(EHP-LRS3)。通过单因素实验和响应面试验对TK-PUL与压热联合制备EHP-LRS3的工艺参数进行了优化,采用扫描电子显微镜和X-射线衍射对EHP-LRS3的形貌、晶体结构进行了观察与分析,并考察了EHP-LRS3对长双歧杆菌的促增殖能力。结果表明,在TK-PUL酶解温度为80℃时制备EHP-LRS3的最佳工艺为:向质量分数为35.32%的莲子淀粉乳(pH5.00)中添加25.00 U/g(莲子淀粉)的TK-PUL,将混合物于80℃处理12.70 h,再将其依次于121℃压热处理10 min、4℃回生处理24 h。在最佳工艺条件下,EHP-LRS3的得率为58.46%。扫描电镜分析显示EHP-LRS3呈不规则的沟壑状结构。X-射线衍射分析表明EHP-LRS3的晶体结构呈现出不同于莲子淀粉A型晶体结构的B型晶体结构。EHP-LRS3对长双歧杆菌的促增殖能力优于压热法制备的RS3型抗性淀粉(HP-LRS3)。本研究采用TK-PUL与压热联合制备得到高得率的RS3型抗性淀粉(EHP-LRS3),并验证了EHP-LRS3对长双歧杆菌的促增殖能力,为莲子淀粉的高值化利用提供了理论依据。

高昌回鹘女子花蕾冠考释

高昌回鹘壁画中有一例特殊冠饰“花蕾冠”,蕴含着独特的服饰文化。运用图像学、历史学等方法,对花蕾冠的造型特征、演变过程、佩戴者身份等级展开探讨。研究表明花蕾冠的造型是以佛教莲花为主要参考物;随着佛教的兴盛,莲花的花瓣与花苞形态也开始演变,花蕾冠由早期出现的“双支菱形”样式逐渐演变为“单只或双支花苞”样式;佩戴人群身份为官员的家人,并非皇室阶层。花蕾冠不仅完善了莲花冠饰体系,也使民族宗教、社会礼俗具象化。

论《金瓶梅词话》饮食叙事的时空特征及其小说史意义

《金瓶梅词话》饮食叙事的时空特征比较明显,一方面,兰陵笑笑生刻画了以自然地理条件为基础的特色饮食物象,时令性与地域性特点突出;另一方面,书中的饮食场景描写深入而细致,展现了西门庆等人的性格特征、生活状态以及世俗人情等多方面的内容。从小说史角度来看,世情小说《金瓶梅词话》的饮食叙事具有开创性意义,在饮食物象选择与场景构建方面对《林兰香》《红楼梦》等作品也有着启迪作用。

佛教莲纹对中国传统织物花卉纹的影响

佛教东传引入的植物装饰纹样,推动了中国古代植物纹饰的发展,其中佛教莲纹的流传极大促进了中国古代莲纹装饰的繁盛。织物上的莲纹,亦在佛教莲纹的流传中逐渐兴起。在其影响下,织物莲纹的造型、色彩,以及莲花与其他物象的组合纹样不断丰富衍变。佛教莲纹模式化的圆花造型,推动了北朝织物上圆花纹饰和唐代宝花纹饰的形成,从而为中国传统织物团花的流行奠定了基础。本土莲纹在吸收、借鉴佛教莲纹的基础上,结合世俗生活,生成许多新的纹样形式和吉祥意象,并在明清吉祥纹样大流行之际广泛运用于织物,促进了中国传统织物纹样的丰富和发展。

花粉辅助合成钴蓝中空微球及性能研究

采用荷花花粉辅助共沉淀法制备球状氢氧化物/花粉前驱体,再经高温烧结制得中空微球钴蓝粉末样品。通过调节反应体系的pH值,探讨制备中空微球钴蓝结构的工艺条件。进一步对钴蓝样品进行表征,并提出中空微球钴蓝形成机理。结果表明:pH值对钴蓝的微观形貌及性能影响较大。当pH值为8.5时,制得形貌可控的中空微球钴蓝样品,微球层内存在二级多孔结构,样品的比表面积和比表面能增加,有助于提高光学性能。其呈色性能较优(L*=40.86,a*=-9.81,b*=-39.26),荷花花粉模板在蓝紫光区450—490 nm波段的反射率比未加花粉模板高出约13%,通过对无机钴蓝颜料性能研究将为化学材料的制备提供新方法。

张竹坡与明清小说文法理论的新发展

明清通俗小说文法论在明末清初金圣叹、毛伦毛宗岗父子、张竹坡等人的手中得以建构与发展。张竹坡在金圣叹的基础上发展了小说文法论,从创作论的角度反向拆解《金瓶梅》的文法,还原作者的构思,注重文法在小说情节推进中动态照应的文章之美,揭示出了《金瓶梅》复合单线乃至初步网状叙事结构,这是其在小说文法论建构上的贡献。张竹坡的文法论建构推动了清代通俗小说的创作和批评,催生出了《红楼梦》的网状叙事结构这一璀璨的明珠。

《金瓶梅》的地域性实证问题

明代的运河码头城市山东临清,“十九皆徽商占籍”。《金瓶梅》中的清河,并不是山东的清河,而是更多有着淮安清河的影子。《金瓶梅》中的清河所指与山东的运河码头临清相混淆。徽商和清河等的书写,皆展现了运河文化的流动性。从文化流动性的维度,解读《金瓶梅》与京杭大运河文化之间的关系,发现不能坐实《金瓶梅》故事中的地理位置、地名;也不能以为故事委实是发生在山东进而通过山东方言尤其是临清方言来印证或考索小说作者的籍贯。《金瓶梅》叙事的地域性实证研究往往忽视了运河文化的流动性而导致缘木求鱼。大运河的交通,促成人口、物质、文化等的流动,而正是这种流动性生成了包括明清小说在内的运河文化。运河文化的流动性解构了小说叙事实证研究的地域性。