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A low-power Rijndael S-Box based on pass transmission gate and composite field arithmetic 被引量:2
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作者 ZENG Yong-hong ZOU Xue-cheng +1 位作者 LIU Zheng-lin LEI Jian-ming 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2007年第10期1553-1559,共7页
Using composite field arithmetic in Galois field can result in the compact Rijndael S-Box. However, the power con- sumption of this solution is too large to be used in resource-limited embedded systems. A full-custom ... Using composite field arithmetic in Galois field can result in the compact Rijndael S-Box. However, the power con- sumption of this solution is too large to be used in resource-limited embedded systems. A full-custom hardware implementation of composite field S-Box is proposed for these targeted domains in this paper. The minimization of power consumption is implemented by optimizing the architecture of the composite field S-Box and using the pass transmission gate (PTG) to realize the logic functions of S-Box. Power simulations were performed using the netlist extracted from the layout. HSPICE simulation results indicated that the proposed S-Box achieves low power consumption of about 130 μW at 10 MHz using 0.25 μm/2.5 V technology, while the consumptions of the positive polarity reed-muller (PPRM) based S-Box and composite field S-Box based on the conventional CMOS logic style are about 240 μW and 420 μW, respectively. The simulations also showed that the presented S-Box obtains better low-voltage operating property, which is clearly relevant for applications like sensor nodes, smart cards and radio frequency identification (RFID) tags. 展开更多
关键词 Composite field Rijndael S-Box FULL-CUSTOM pass transmission gate (PTG) low power consumption low-VOLTAGE
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Memristor bridge-based low pass filter for image processing 被引量:5
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作者 YU Yongbin YANG Nijing +1 位作者 YANG Chenyu NYIMA Tashi 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2019年第3期448-455,共8页
This paper highlights the memristor bridge-based lowpass filter (LPF) and improved image processing algorithms along with a novel adaptive Gaussian filter for denoising image and a new Gaussian pyramid for scale invar... This paper highlights the memristor bridge-based lowpass filter (LPF) and improved image processing algorithms along with a novel adaptive Gaussian filter for denoising image and a new Gaussian pyramid for scale invariant feature transform (SIFT). First, a novel kind of LPF based on the memristor bridge is designed, whose cut-off frequency and other traits are demonstrated to change with different time and memristance. In light of the changeable parameter of the memristor bridge-based LPF, a new adaptive Gaussian filter and an improved SIFT algorithm are presented. Finally, experiment results show that the peak signalto- noise ratio (PSNR) of our denoising is bettered more than 2.77 dB compared to the corresponding of the traditional Gaussian filter, and our improved SIFT performances including the number of matched feature points and the percent of correct matches are higher than the traditional SIFT, which verifies feasibility and effectiveness of our algorithm. 展开更多
关键词 MEMRISTOR BRIDGE low-pass FILTER (LPF) adaptive GAUSSIAN FILTER image denoising GAUSSIAN pyramid
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Compact Low Pass Filter Design for L-Band Application
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作者 Dhirendra Kumar Asok De 《Journal of Electromagnetic Analysis and Applications》 2011年第4期115-117,共3页
An effective technique to design compact low pass filter has been proposed in this paper. The proposed method is highly effective for L-band applications. Low impedance microstrip lines are arranged such that they wor... An effective technique to design compact low pass filter has been proposed in this paper. The proposed method is highly effective for L-band applications. Low impedance microstrip lines are arranged such that they work as open stubs to increase the selectivity of the filter. Using the proposed technique about 57% size reduction has been realized with sharper roll off characteristics. An empirical expression is derived to determine the dimension of resonators. For cut-off frequency of 1.7 GHz the investigated method has been fabricated and tested. There is a close agreement be-tween simulated and measured 展开更多
关键词 low pass Filter CHEBYSHEV PROTOTYPE Stepped IMPEDANCE ETC
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Design of a compact low-pass filter with wide stopband 被引量:1
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作者 杨雪霞 薛玉杰 《Journal of Shanghai University(English Edition)》 CAS 2008年第6期495-497,共3页
This paper presents a novel low-pass filter (LPF) with sharp rejection, wide stopband and compact size, which are realized by the defected ground structure (DGS) and the defected microstrip structure (DMS). The ... This paper presents a novel low-pass filter (LPF) with sharp rejection, wide stopband and compact size, which are realized by the defected ground structure (DGS) and the defected microstrip structure (DMS). The equivalent circuit model is proposed and the circuit parameters are extracted by the circuit simulation software. The parameters measured are 3 dB cutoff frequency fc of 5.2 GHz, the insertion loss less than 0.5 dB from DC to 4.0 GHz and S21 less than -20 dB within the wide stopband from 6 GHz to 16 GHz. The results of the circuit optimization agree well with those of the full wave simulation and the measured ones, which validate the effectiveness of the equivalent circuit model. The size of the proposed LPF is decreased compared with normal LPF. This LPF can be applied in rectennas to eliminate high order harmonics. 展开更多
关键词 low-pass filter (LPF) defected ground structure (DGS) defected microstrip structure (DMS) equivalent circuit
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DAPSO and PSO-VAF in Linear Phase Digital Low Pass FIR Filter Design
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作者 Mudit Shukla G. R. Mishra 《Circuits and Systems》 2014年第3期57-67,共11页
Digital filters play a key role in the field of digital signal processing. This paper presents a linear phase digital low pass finite impulse response (FIR) filter design using particle swarm optimization and its two ... Digital filters play a key role in the field of digital signal processing. This paper presents a linear phase digital low pass finite impulse response (FIR) filter design using particle swarm optimization and its two new variants, dynamic and adjustable particle swarm optimization (DAPSO) and particle swarm optimization with variable acceleration factor (PSO-VAF) and illustrates the superiority of the PSO-VAF method over PSO based methods. Two fitness functions are considered. The fitness1 is used to find the possible minimum ripples in pass band and stop band in case of PSO, DAPSO and PSO-VAF. Fitness2 is able to control the ripples in both bands separately. A comparison of simulation results demonstrates the performance of PSO and its methods in designing digital low pass FIR filters. 展开更多
关键词 DIGITAL FILTERS Design low pass FIR FILTERS PSO DAPSO PSO-VAF
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Design of Sixth-Order Lowpass Elliptic Switched-Capacitor Filter
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作者 Dong Yan Liang Yin +2 位作者 Xiaowei Liu Wenning Jiang Qiuye Lv 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2016年第6期47-53,共7页
In this paper the design and implementation of sixth-order lowpass elliptic switched-capacitor filter( SCF) for interface circuit of Micro-Electro-Mechanical System( MEMS) sensor are presented. This work aims to lower... In this paper the design and implementation of sixth-order lowpass elliptic switched-capacitor filter( SCF) for interface circuit of Micro-Electro-Mechanical System( MEMS) sensor are presented. This work aims to lower total harmonic distortion( THD) without deteriorating other performances. After system design in Simulink,the filter is realized in transistor level and finally fabricated in Central Semiconductor Manufacturing Corporation( CSMC) 0.5 μm metal-oxide-semiconductor( CMOS) technology. Typical measured results are: it operates with 25: 1 clock-to-corner frequency ratio and a 10 k Hz maximum corner frequency. The maximum passband ripple is about 0.49 d B and the minimum stopband rejection is 40 d B for the temperature from-20 ℃to 80 ℃. For the 250 k Hz clock frequency setting,given the 1 k Hz,- 8 d BVrms input signal,the measured worst case THD is-64 d B. The active area of the chip is 2.8 mm2 with 8 pads. The analog power dissipation is10 m W from a 5 V power supply. 展开更多
关键词 SWITCHED-CAPACITOR lowpass elliptic filter biquad
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Design of low-pass filter based on a novel defected ground structure
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作者 钟小明 李国辉 《Journal of Shanghai University(English Edition)》 CAS 2007年第4期396-399,共4页
A novel defected ground structure (DGS) for the microstrip line is proposed in this paper. The DGS lattice has more defect parameters so that it can provide better performance than the conventional dumbbell-shaped D... A novel defected ground structure (DGS) for the microstrip line is proposed in this paper. The DGS lattice has more defect parameters so that it can provide better performance than the conventional dumbbell-shaped DGS. Selectivity is improved by 97.2% with a sharpness factor of 24.6%. The method is applied to the design of a low-pass filter to confirm validity of the proposed DGS. 展开更多
关键词 defected ground structure (DGS) low-pass filter bandgap sharpness factor SELECTIVITY
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Microstrip Low-Pass Elliptic Filter Design Based on Implicit Space Mapping Optimization
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作者 Saeed Tavakoli Mahdieh Zeinadini Shahram Mohanna 《International Journal of Communications, Network and System Sciences》 2010年第5期462-465,共4页
It is a time-consuming and often iterative procedure to determine design parameters based on fine, accurate but expensive, models. To decrease the number of fine model evaluations, space mapping techniques may be empl... It is a time-consuming and often iterative procedure to determine design parameters based on fine, accurate but expensive, models. To decrease the number of fine model evaluations, space mapping techniques may be employed. In this approach, it is assumed both fine model and coarse, fast but inaccurate, one are available. First, the coarse model is optimized to obtain design parameters satisfying design objectives. Next, auxiliary parameters are calibrated to match coarse and fine models’ responses. Then, the improved coarse model is re-optimized to obtain new design parameters. The design procedure is stopped when a satisfactory solution is reached. In this paper, an implicit space mapping method is used to design a microstrip low-pass elliptic filter. Simulation results show that only two fine model evaluations are sufficient to get satisfactory results. 展开更多
关键词 IMPLICIT Space Mapping OPTIMIZATION MICROSTRIP low-pass ELLIPTIC FILTER Surrogate Model
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Compact Metamaterial Microstrip Low-Pass Filter
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作者 Sudhakar Sahu Rabindra Kishore Mishra Dipak Ranjan Poddar 《Journal of Electromagnetic Analysis and Applications》 2011年第10期399-405,共7页
Complimentary hexagonal-omega structures are used to design compact, low insertion loss (IL), low pass filter with sharp cut-off. It has been designed for improvement of roll-off performance based on both μ and ε ne... Complimentary hexagonal-omega structures are used to design compact, low insertion loss (IL), low pass filter with sharp cut-off. It has been designed for improvement of roll-off performance based on both μ and ε negative property of the complimentary hex-omega structure while maintaining the filter pass-band performance. By properly designing and loading the hexagonal-omega structure in the ground of microstrip line not only improve the roll-off of the low pass filter, but also reduced the filter size. The simulated results indicate that the proposed filter achieves a flat pass band with no ripples as well as selectivity of 19.68 dB/GHz, corresponding to 5-unit cells hex-omega structures. This significantly exceeds the 5.6 dB/GHz selectivity of the conventional low pass filter design, due to sub-lambda dimensions of the hex-omega structure. A prototype filter implementing area is: 0.712λg x 0.263λg, λg being the guided wavelength at 3-dB cut-off frequency (fc). The proposed filter has a size smaller by 36.2%. 展开更多
关键词 Hex-Omega Structure MICROSTRIP low-pass Filter METAMATERIAL Sharp CUT-OFF
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CD34+/CD38low/-/CD123+细胞表达水平对急性髓系白血病预后的意义 被引量:5
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作者 向立丽 邱国强 +3 位作者 谢晓宝 岑建农 胡绍燕 顾伟英 《中国实验血液学杂志》 CAS CSCD 北大核心 2017年第1期61-65,共5页
目的:探讨急性髓系白血病(AML)患者CD34^+/CD38^(low/-)/CD123^+细胞表达水平,并分析CD34^+/CD38^(low/-)/CD123^+表达水平与预后的相关性。方法:采用流式细胞术检测148例初诊AML患者骨髓单个核细胞中CD34^+/CD38^(low/-)/CD123^+表达水... 目的:探讨急性髓系白血病(AML)患者CD34^+/CD38^(low/-)/CD123^+细胞表达水平,并分析CD34^+/CD38^(low/-)/CD123^+表达水平与预后的相关性。方法:采用流式细胞术检测148例初诊AML患者骨髓单个核细胞中CD34^+/CD38^(low/-)/CD123^+表达水平,分析CD34^+/CD38^(low/-)/CD123^+细胞表达水平与完全缓解率、无病生存率和总体生存率相关性。结果:初诊AML患者骨髓细胞中CD34^+/CD38^(low/-)/CD123^+阳性细胞中位表达率为2.8%(0.01%-67%)。AML患者CD34^+/CD38^(low/-)/CD123^+高表达与NPM1野生型呈正相关(x^2=5.194,P=0.023),而与FLT3-ITD突变阳性无相关性(x^2=0.418,P>0.05)。多变量回归分析显示,CD34^+/CD38^(low/-)/CD123^+高表达与较低的完全缓解率(P=0.035)、较差的无病生存(P<0.001)、较短的总生存(P<0.001)相关。结论:CD34^+/CD38^(low/-)/CD123^+表达率与AML患者治疗的反应及生存密切相关,在临床中可做为快速识别患者治疗失败的风险预后指标。 展开更多
关键词 急性髓系白血病 CD34+/CD38low/-/CD123+ 预后
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CD4^+CD25^+CD127^(low/-)调节性T细胞在晚期胃癌中的表达及意义 被引量:13
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作者 钟锐 冯龙 +3 位作者 兰琼玉 易烽明 蒋为民 吴建兵 《重庆医学》 CAS 北大核心 2016年第15期2065-2069,共5页
目的研究CD4^+CD25^+CD127low/-调节性T细胞(Treg)在晚期胃癌患者外周血中的表达水平及其对化疗疗效判断的意义。方法采用流式细胞术(FCM)检测30例晚期胃癌患者和10例健康体检者外周血中CD4^+CD25^+CD127low/-Treg的表达水平。结果晚期... 目的研究CD4^+CD25^+CD127low/-调节性T细胞(Treg)在晚期胃癌患者外周血中的表达水平及其对化疗疗效判断的意义。方法采用流式细胞术(FCM)检测30例晚期胃癌患者和10例健康体检者外周血中CD4^+CD25^+CD127low/-Treg的表达水平。结果晚期胃癌患者外周血中CD4^+CD25^+CD127low/-Treg占CD4^+T淋巴细胞的百分比为(10.41±2.85)%,明显高于健康体检者(5.05±1.32)%(t=8.04,P<0.01),也明显高于化疗2周期后(7.07±1.45)%,(t=5.72,P<0.01)和化疗4周期后(7.30±1.65)%(t=5.17,P<0.01)。晚期胃癌患者化疗2周期后外周血中CD4^+CD25^+CD127low/-Treg占CD4^+T淋巴细胞的百分比为(7.07±1.45)%,与化疗4周期后(7.30±1.65)%比较,差异无统计学意义(t=0.57,P>0.05)。晚期胃癌患者外周血中CD4^+CD25^+CD127low/-Treg表达水平与患者性别、年龄、胃癌相关肿瘤抗原及肿瘤部位无相关性(P>0.05),低分化程度与中分化程度差异无统计学意义(P>0.05)。晚期胃癌患者化疗2周期后缓解组(完全缓解^+部分缓解)外周血CD4^+T细胞中CD4^+CD25^+CD127low/-Treg的水平为(6.25±1.08)%与化疗2周期后未缓解组(病变稳定^+疾病进展)的(7.98±1.27)%比较,差异有统计学意义(t=4.02,P<0.01)。结论晚期胃癌患者外周血中CD4^+CD25^+CD127low/-Treg的表达水平与肿瘤缓解情况有关。 展开更多
关键词 胃肿瘤 T淋巴细胞 流式细胞术 化疗 晚期胃癌 CD4+CD25+CD127low/-调节性T细胞
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Complementary Pass-Transistor Adiabatic Logic Circuit Using Three-Phase Power Supply 被引量:1
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作者 胡建平 邬杨波 张卫强 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2004年第8期918-924,共7页
A new low power quasi adiabatic logic,complementary pass transistor adiabatic logic (CPAL),is presented.The CPAL circuit is driven by a new three phase power clock,and its non adiabatic loss on output loads can b... A new low power quasi adiabatic logic,complementary pass transistor adiabatic logic (CPAL),is presented.The CPAL circuit is driven by a new three phase power clock,and its non adiabatic loss on output loads can be effectively reduced by using complementary pass transistor logic and transmission gates.Furthermore,the minimization of the energy consumption can be obtained by choosing the optimal size of bootstrapped nMOS transistors,thus it has more efficient energy transfer and recovery.A three phase power supply generator with a small control logic circuit and a single inductor is proposed.An 8 bit adder based on CPAL is designed and verified.With MOSIS 0 25μm CMOS technology,the CPAL adder consumes only 35% of the dissipated energy of a 2N 2N2P adder and is about 50% of the dissipated energy of a PFAL adder for clock rates ranging from 50 to 200MHz. 展开更多
关键词 complementary pass transistor logic adiabatic logic low power 3 phase power clock generator
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E-cadherin、N-cadherin与CD44^+/CD24^(-/low)表型在乳腺癌中表达的相关性及其意义 被引量:16
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作者 包俊杰 吴诚义 《中国癌症杂志》 CAS CSCD 北大核心 2010年第8期596-601,共6页
背景与目的:上皮细胞-间质转化(epithelial-mesenchymal transition,EMT)可以诱导上皮细胞来源的肿瘤表达CD44+/CD24-/low表型细胞,上皮性钙黏蛋白(E-cadherin)和神经性钙黏蛋白(N-cadherin)是分别表达于上皮细胞和间质细胞的两个重要... 背景与目的:上皮细胞-间质转化(epithelial-mesenchymal transition,EMT)可以诱导上皮细胞来源的肿瘤表达CD44+/CD24-/low表型细胞,上皮性钙黏蛋白(E-cadherin)和神经性钙黏蛋白(N-cadherin)是分别表达于上皮细胞和间质细胞的两个重要钙黏蛋白,癌细胞从E-cadherin表达向N-cadherin表达转移是EMT的一个重要机制,此钙黏蛋白转移增加了癌细胞的侵袭转移性。本研究旨在探讨CD44+/CD24-/low表型、E-cadherin及N-cadherin在乳腺癌中的表达及其意义。方法:采用免疫组织化学检测15例正常乳腺组织、20例乳腺癌癌旁组织(癌边缘2~5cm)、58例乳腺癌组织及18例腋窝转移淋巴结组织中CD44+/CD24-/low表型、E-cadherin及N-cadherin的表达,分析其与乳腺癌发生及浸润转移的关系。结果:正常乳腺组织、乳腺癌癌旁组织、乳腺癌组织和腋窝转移淋巴结组织中CD44+/CD24-/low表型表达率分别为6.7%、10.0%、41.4%和44.4%,E-cadherin阳性表达率分别为100%、90.0%、51.7%和33.3%,N-cadherin阳性表达率分别为0、15.0%、44.8%和55.6%,3种蛋白在正常乳腺组织、乳腺癌癌旁组织、乳腺癌组织和腋窝转移淋巴结组织中的表达差异均有统计学意义(P<0.05)。CD44+/CD24-/low表型表达与E-cadherin及N-cadherin表达有显著差异,且与E-cadherin表达关联程度较强,与N-cadherin表达关联程度相对较弱。E-cadherin表达与N-cadherin表达有显著差异,且具有高度相关性(P<0.05)。结论:CD44+/CD24-/low表型表达增加、E-cadherin蛋白表达降低及N-cadherin蛋白反常表达与乳腺癌的发生及浸润转移有密切的关系;在乳腺癌发展过程中,E-cadherin向N-cadherin转化及CD44+/CD24-/low表型表达增加可能发挥重要作用。 展开更多
关键词 乳腺癌 CD44+/CD24-/low表型 E-CADHERIN蛋白 N-cadherin蛋白 双重免疫组织 化学染色
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B淋巴细胞白血病骨髓B细胞CD34^+CD38^+和CD34^+CD38^(low/-)细胞亚群的临床意义 被引量:3
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作者 郝乐 刘艳荣 +5 位作者 王亚哲 常艳 秦亚溱 李金兰 李玲娣 黄晓军 《中国实验血液学杂志》 CAS CSCD 北大核心 2012年第4期801-805,共5页
本研究比较发病时免疫表型为CD34+CD38+和CD34+CD38low/-两组B淋巴细胞白血病(B-ALL)的生物学特性,并探讨其临床意义。选取54例初发B-ALL并经多参数流式细胞术检测为CD34+的B-ALL患者,根据CD38表达不同将其分为CD34+CD38+组(n=35)和CD34... 本研究比较发病时免疫表型为CD34+CD38+和CD34+CD38low/-两组B淋巴细胞白血病(B-ALL)的生物学特性,并探讨其临床意义。选取54例初发B-ALL并经多参数流式细胞术检测为CD34+的B-ALL患者,根据CD38表达不同将其分为CD34+CD38+组(n=35)和CD34+CD38low/-组(n=19)。利用实时定量PCR的方法分别进行BCR-ABL,TEL-AML1和WT1基因的检测。随访标本利用7色流式细胞术检测微量残留病(MRD),平均随访时间12个月(1-28个月),平均随访间隔2个月(1-5个月)。结果表明,两组患者发病时WBC,血小板和血红蛋白水平及BCR-ABL,TEL-AML1和WT1基因阳性表达的比例均无统计学差异(P>0.05)。在诱导缓解后,CD34+CD38+组微量残留病阳性(MRD+)为28.57%(10/35),CD38low/-组MRD+为68.42%(13/19),CD34+CD38low/-组MRD+的检出率明显高于CD34+CD38+组(P<0.01)。在复发率上,CD34+CD38+组有2例,分别在第94天和第245天复发,复发率为5.71%(2/35)。CD34+CD38low/-组有7例复发,复发率为36.84%(7/19),中位复发时间263 d(46-468 d),两组间具有明显统计学差异(P<0.01)。本研究以16岁为年龄分界点,CD34+CD38+组16岁以上患者为8人(8/35),CD34+CD38low/-组16岁以上患者为10人(10/19),两组间有统计学差异(P<0.05)。结论:CD34+CD38low/-患者在成人中比例较高,且CD34+CD38low/-组在治疗后更易出现MRD+和复发。 展开更多
关键词 CD34+CD38+ CD34+CD38low/- B淋巴细胞白血病 微小残留病
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纳米TiO_2-PASS复合絮凝剂的制备及其性能 被引量:2
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作者 王志刚 王丽贤 刘贵平 《纺织高校基础科学学报》 CAS 2014年第4期525-530,共6页
采用溶胶-凝胶法制备了纳米TiO2-PASS复合絮凝剂,对所得复合絮凝剂的成分、结构与光催化性能的关系进行研究,并采用XRD、FI-IR对样品进行表征.结果表明,TiO2-PASS复合絮凝剂的最佳制备条件为PASS用量2mL、钛酸丁酯用量5mL、无水乙醇用量... 采用溶胶-凝胶法制备了纳米TiO2-PASS复合絮凝剂,对所得复合絮凝剂的成分、结构与光催化性能的关系进行研究,并采用XRD、FI-IR对样品进行表征.结果表明,TiO2-PASS复合絮凝剂的最佳制备条件为PASS用量2mL、钛酸丁酯用量5mL、无水乙醇用量24mL、冰乙酸用量1mL、去离子水用量3mL、浓盐酸用量0.3mL.光催化实验结果表明,当复合絮凝剂煅烧温度500℃、模拟染料废水pH=2.0、光照时间120min时光催化性能最佳,活性艳兰废水的脱色率达到95%.XRD、FI-IR图谱表明PASS掺杂的样品晶型均为锐钛矿型,PASS的掺杂改变了TiO2晶相转变温度,提高了TiO2晶体结晶度和粒子尺寸,有利于复合絮凝剂性能的发挥. 展开更多
关键词 溶胶-凝胶法 纳米TIO2 pass掺杂 光催化
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CD4^+ CD25^(high) CD127^(low/-)调节性T细胞在多发性骨髓瘤患者外周血的表达 被引量:3
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作者 高鹏 王雪野 +6 位作者 肖中平 付堃 孔令环 王雪梅 李凌君 封韬 韩梅 《中国老年学杂志》 CAS CSCD 北大核心 2014年第20期5673-5675,共3页
目的探讨多发性骨髓瘤(MM)患者外周血CD4+CD25highCD127low/-调节性T(Tregs)细胞表达水平及临床意义。方法采用流式细胞术(FCM)检测30例MM患者和20例健康对照者外周静脉血CD4+CD25highCD127low/-Tregs细胞水平。结果 1 MM组CD4+CD25high... 目的探讨多发性骨髓瘤(MM)患者外周血CD4+CD25highCD127low/-调节性T(Tregs)细胞表达水平及临床意义。方法采用流式细胞术(FCM)检测30例MM患者和20例健康对照者外周静脉血CD4+CD25highCD127low/-Tregs细胞水平。结果 1 MM组CD4+CD25high CD127low/-Tregs细胞表达明显高于正常对照组(P<0.05)。2MM组不同国际分期系统(ISS)分期CD4+CD25highCD127low/-Treg细胞水平均高于正常对照组,随着疾病进展,Ⅲ期明显高于Ⅱ期(P<0.05)。3MM组化疗后外周血CD4+CD25highCD127low/-Tregs细胞水平较化疗前显著下降(P<0.05)。结论 MM患者外周血CD4+CD25highCD127low/-Treg细胞比例明显升高,可能是MM免疫逃逸的一个重要机制;进展期Tregs的变化为MM患者及时进行临床干预提供实验依据。 展开更多
关键词 多发性骨髓瘤 CD4+CD25highCD127low/-调节性T细胞 流式细胞术
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慢性乙型肝炎肝纤维化患者外周血CD4^+CD25^+CD127^(low/-) T细胞与人肝星状细胞共培养的实验研究 被引量:4
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作者 金晓芝 陈永平 +4 位作者 程瑗 潘黎静 潘陈为 戴志娟 叶超 《医学研究杂志》 2011年第8期70-73,共4页
目的探讨CD4+CD25+CD127low/-T细胞在慢性乙型病毒性肝炎肝纤维化发病机制中的作用。方法按肝纤维化S分期将慢性乙型肝炎肝纤维化患者分为S1~S2期6例和S3~S4期7例;采用磁激活细胞分选法(MACS)提取13例患者和6例健康对照者外周血CD4+CD25... 目的探讨CD4+CD25+CD127low/-T细胞在慢性乙型病毒性肝炎肝纤维化发病机制中的作用。方法按肝纤维化S分期将慢性乙型肝炎肝纤维化患者分为S1~S2期6例和S3~S4期7例;采用磁激活细胞分选法(MACS)提取13例患者和6例健康对照者外周血CD4+CD25+CD127low/-T细胞;分别将CD4+CD25+CD127low/-T细胞以不同比例与人肝星状细胞株(hepaticstellate cell,HSC)共培养5天,检测各组HSC的增殖及TGF-β1的分泌。结果 CD4+CD25+CD127low/-T细胞与HSC之比,当健康对照组和S1~S2期组为1.5∶1,S3~S4期组为2∶1时,HSC的增殖速度和TGF-β1分泌量均在同组不同比例中达最高值。各组同比例之间,当比例为1.5∶1时,健康对照组HSC的增殖速度显著高于S3~S4期组(P<0.05);在比例为1∶1~2∶1范围内,S1~S2期组和S3~S4期组HSC的TGF-β1分泌量远高于同比例健康对照组(P<0.05)。结论 CD4+CD25+CD127low/-T细胞可能通过自身细胞数量和功能的双重调节,影响肝星状细胞的增殖和分泌,参与了慢乙肝肝纤维化的进展。 展开更多
关键词 慢性乙型病毒性肝炎 肝纤维化 CD4+CD25+CD127low/- Treg MACS
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HIV感染者外周血CD4^+ CD25^+ Foxp3^+ /CD127^(low/-)调节性T细胞表达水平对疾病进展的影响 被引量:6
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作者 陈远林 陈新瑞 +2 位作者 秦立新 杨一芬 唐亚梅 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第11期1188-1191,1193,共5页
目的:探讨HIV感染者外周血中CD4+CD25+Foxp3+调节性T细胞(Treg)、CD4+CD25+CD127low/-Treg的水平及其与其他免疫指标的关系。方法:采集68例未经抗HIV治疗的HIV/AIDS患者(长期不进展组即LTNP组29例、典型进展的HIV感染组27例、AIDS组12例... 目的:探讨HIV感染者外周血中CD4+CD25+Foxp3+调节性T细胞(Treg)、CD4+CD25+CD127low/-Treg的水平及其与其他免疫指标的关系。方法:采集68例未经抗HIV治疗的HIV/AIDS患者(长期不进展组即LTNP组29例、典型进展的HIV感染组27例、AIDS组12例)及20例健康成人的外周抗凝全血,经免疫荧光染色,应用流式细胞仪分析CD4+T细胞、CD8+T细胞、NK细胞及CD4+CD25+Foxp3+/CD127low/-Treg的含量,并进行统计学分析。结果:除CD8+T细胞外,HIV/AIDS患者外周血中CD4+T细胞、NK细胞、CD4+/CD8+结果均明显低于健康对照组(P<0.05);随着疾病的进展,LTNP组、HIV组、AIDS组CD4+T细胞百分比、绝对值计数,CD8+T细胞绝对计数,NK细胞绝对计数,CD4+/CD8+比值逐渐下降,而CD8+T细胞百分比逐渐上升。对CD4+CD25+Foxp3+Treg与CD4+CD25+CD127low/-Treg百分含量、绝对计数进行多重比较发现,各组间CD4+CD25+Foxp3+Treg与CD4+CD25+CD127low/-Treg所占CD4+T细胞百分含量的差异均有统计学意义(P<0.05),并且随着疾病的发展,CD4+CD25+Foxp3+/CD127low/-Treg细胞百分含量逐渐上升,LTNP组与健康对照组之间、HIV组和AIDS组之间CD4+CD25+Foxp3+/CD127low/-Treg绝对计数差异无统计学意义(P>0.05),其余各组间的差异均有统计学意义(P<0.05),并且随着疾病的发展,CD4+CD25+Foxp3+/CD127low/-Treg绝对计数逐渐下降。结论:CD4+CD25+Foxp3+/CD127low/-Treg在HIV持续感染的免疫发病机制中有一定作用。 展开更多
关键词 HIV CD4+CD25+Foxp3+调节性T细胞 CD4+CD25+CD127low/-调节性T细胞 长期不进展者
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三阴乳腺癌CD44^+/CD24^(-/LOW)细胞与CXCR4表达的临床意义 被引量:1
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作者 何惠华 杨继洲 +1 位作者 李蓓 敖启林 《中国组织化学与细胞化学杂志》 CAS CSCD 2014年第2期190-194,共5页
目的探讨CD44+/CD24-/low表型在三阴乳腺癌中的临床病理意义以及与CXCR4表达的相关性。方法根据ER、PR、Her-2表达情况将118例乳腺癌分为三阴及非三阴组,应用免疫组织化学检测其CD44、CD24及CXCR4表达情况,分析CD44+/CD24-/low表型及CX... 目的探讨CD44+/CD24-/low表型在三阴乳腺癌中的临床病理意义以及与CXCR4表达的相关性。方法根据ER、PR、Her-2表达情况将118例乳腺癌分为三阴及非三阴组,应用免疫组织化学检测其CD44、CD24及CXCR4表达情况,分析CD44+/CD24-/low表型及CXCR4在三阴乳腺癌中表达情况及其与临床病理的关系。结果 34例三阴乳腺癌肿瘤细胞CD44+/CD24-/low表型阳性率为55.9%,CD44+/CD24-/low阳性与一般临床特征不相关,但与远处转移或复发密切相关。CXCR4的表达与CD44+/CD24-/low阳性细胞成正相关。结论三阴乳腺癌的复发和远处转移可能与CD44+/CD24-/low较高的表达率有关,并且可能是由CXCR4介导的。 展开更多
关键词 三阴乳腺癌 CD44+/CD24-/low表型细胞 CXCR4蛋白 随访
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类风湿关节炎患者CD4^+CD25^+CD127^(LOW/-)调节性T细胞检测及意义 被引量:4
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作者 张智贤 曾桦 《检验医学与临床》 CAS 2013年第22期2984-2985,2987,共3页
目的研究CD4+CD25+CD127 LOW/-调节性T细胞(Tregs)在类风湿关节炎患者(RA)外周血中的比例改变,并探讨其在疾病进程中的意义。方法选取活动期及稳定期RA患者,采用流式细胞术,检测外周血中CD4+CD25+CD127 LOW/-Treg细胞占CD4+细胞的比例,... 目的研究CD4+CD25+CD127 LOW/-调节性T细胞(Tregs)在类风湿关节炎患者(RA)外周血中的比例改变,并探讨其在疾病进程中的意义。方法选取活动期及稳定期RA患者,采用流式细胞术,检测外周血中CD4+CD25+CD127 LOW/-Treg细胞占CD4+细胞的比例,并与健康者进行比较。结果 RA患者CD4+CD25+细胞所占比例与健康对照组差异无统计学意义;而活动期患者外周血CD4+CD25+CD127 LOW/-Treg细胞明显低于稳定期组和健康对照组(P<0.05)。结论类风湿性关节炎活动期时CD4+CD25+CD127 LOW/-Treg细胞明显减少,这群调节性T细胞可能参与了类风湿性关节炎的疾病进程。 展开更多
关键词 I类风湿性关节炎 流式细胞术 CD4+CD25+CD127low -调节性T细胞
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