Genetic Transformation of Watermelon with Pumpkin DNA by Low Energy Ion Beam-mediated Introduction

The No.601 watermelon (citrullus lanatus) seeds were treated with 25 keV N+ implantation at the dosage of 7.8 ×1016 ions/cm2. After treatment, watermelon seeds were incubated with 380μg/μl pumpkin (Cucubita, maxima Duch) DNA solution at 35 ℃ for 5 hours. By two-generations of selection and resistance screening at seedling stage, one transformed material was selected out, whose rind color is similar to that of the donor pumpkin and whose size of seeds is between that of the donor and the receptor. Using AFLP (amplified fragment length polymorphism) technique, two polymorphic DNA fragments were amplified. This primarily testified that the donor DNA fragments/gene were introduced into the receptor cell and integrated into the genomic DNA of the receptor.

Bioeffects of Low Energy Ion Beam Implantation:DNA Damage,Mutation and Gene Transter

Low-energy ion beam implantation （10 - 200 keV） has been proved to have a wide range of biological effects and is broadly used in the breeding of crops and micro-organisms.To understand its mechanisms better and facilitate its applications, the developments in the bioeffects of low energy ion beam implantation in the past twenty years are summarized in this paper.

Preliminary Studies on Base Substitutions and Repair of DNA Mismatch Damage Stimulated by Low Energy N^+ Ion Beam Implantation in Escherichia coli

Ever since the low energy N+ ion beam has been accepted that the mutation effects of ionizing radiation are attributed mainly to direct or indirect damage to DNA. Evidences based on naked DNA irradiation in support of a mutation spectrum appears to be consistent, but direct proof of such results in vivo are limited. Using mutS, dam and/or dcm defective Eschericha coli imitator strains, an preliminary experimental system on induction of in vivo mutation spectra of low energy N+ ion beam has been established in this study. It was observed that the mutation rates of rifampicin resistance induced by N+ implantation were quite high, ranging from 9.2 x 10~8 to 4.9× 10~5 at the dosage of 5.2×1014 ions/cm2. Strains all had more than 90-fold higher mutation rate than its spontaneous mutation rate determined by this method. It reveals that base substitutions involve in induction of mutation of low energy nitrogen ion beam implantation. The mutation rates of mutator strains were nearly 500-fold (GM2929), 400-fold (GM5864) and 6-fold larger than that of AB1157. The GM2929 and GM5864 both lose the ability of repair DNA mismatch damage by virtue of both dam and dcm pathways defective (GM2929) or failing to assemble the repair complex (GM5864) respectively. It may explain the both strains had a similar higher mutation rate than GM124 did. It indicated that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N+ implantation. The further related research were also discussed.

A segmented conical electric lens for optimization of the beam spot of the low-energy muon facility at PSI: a Geant4 simulation analysis

The low-energy muon facility at PSI provides nearly fully polarized positive muons with tunable energies in the ke V range to carry out muon spin rotation(LE-μSR)experiments with nanometer depth resolution on thin films,heterostructures, and near-surface regions. The low-energy muon beam is focused and transported to the sample by electrostatic lenses. In order to achieve a minimum beam spot size at the sample position and to enable the steering of the beam in the horizontal and vertical direction, a special electrostatic device has been implemented close to the sample position. It consists of a cylinder at ground potential followed by four conically shaped electrodes,which can be operated at different electric potential. In LE-μSR experiments, an electric field at the sample along the beam direction can be applied to accelerate/decelerate muons to different energies(0.5–30 keV). Additionally, a horizontal or vertical magnetic field can be superimposed for transverse or longitudinal field μSR experiments. The focusing properties of the conical lens in the presence of these additional electric and magnetic fields have been investigated and optimized by Geant4 simulations. Some experimental tests were also performed and show that the simulation well describes the experimental setup.

Electron-beam radiation effects on the structure and properties of polypropylene at low dose rates

While the high-energy radiation effects on polypropylene, which are crucial for the cable industry for nuclear power plants, have been thoroughly studied, the property changes of PP at low-dose-rate electron-beam irradiation are far from elucidated. Herein, the influence of electron-beam irradiation on the structure and properties of PP was examined. The static EB irradiation conditions were 1.2 MeV at a low dose rate of 20 kGy/h to achieve absorbed doses ranging from 45, to 60, 100, and 200 kGy.The molecular structure was first evaluated by measuring the carboxyl index and the relative radical concentrations via Fourier transform infrared spectroscopy and electron spin resonance, respectively. Mechanical, differential scanning colorimetric, and rheological tests were carried out to further investigate the changes in the properties(tensile, crystalizing, and viscoelastic properties) of irradiated PP, which showed good agreement with the structural analysis results. We found that radio-oxidative degradation(chain scission) was predominant, which can be due to the low dose rate facilitating oxygen diffusion into the PP matrix during electron-beam irradiation.

Impact of Low-Energy Ion Beam Implantation on the Expression of Ty1-copia-like Retrotransposons in Wheat(Triticum aestivum)

Retrotransposon-like elements are major constituents of most eukaryotic genomes. For example, they account for roughly 90% of the wheat （Triticum aestivum） genome, Previous study on a wheat strain treated by low-energy N^＋ ions indicated the variations in AFLP （Amplified Fragment Length Polymorphism ） markers, One such variation was caused by the re-activation of Tyl-copia-like retrotransposons, implying that the mutagenic effects of lowenergy ions might work through elevated activation of retrotransposons, In this paper an expression profile of Tyl-copia-like retrotransposons in wheat treated by low-energy N^＋ ions is reported, The reverse transcriptase （RT） domains of these retrotransposons were amplified by reverse-transcriptional polymerase chain reaction （RT-PCR） and sequentially cloned, 42 and 65 clones were obtained from the treated （CL） and control materials （CK）, respectively, Sequence analysis of each clone was performed by software. Phylogeny and classification were calculated responding to the sequences of the RT domains. All the results show that there is much difference in the RT domain between the control sample and the treated sample, Especially, the RT domains from the treated group encode significantly more functional ORF （open reading frames） than those from the control sample, This observation suggests that the treated sample has higher activation of retrotransposons, possibly as a consequence of low-energy ion beam irradiation, It also suggests that retrotransposons in the two groups impact the host gene expression in two different ways and carry out different functions in wheat cells.

The feasibility and safety of high-intensity focused ultrasound combined with low-dose external beam radiotherapy as supplemental therapy for advanced prostate cancer following hormonal therapy

The aim of this study was to investigate the feasibility and safety of high-intensity focused ultrasound （HIFU） combined with （＋） low-dose external beam radiotherapy （LRT） as supplemental therapy for advanced prostate cancer （PCa） following hormonal therapy （HT）. Our definition of HIFU＋LRT refers to treating primary tumour lesions with HIFU in place of reduced field boost irradiation to the prostate, while retaining four-field box irradiation to the pelvis in conventional-dose external beam radiotherapy （CRT）. We performed a prospective, controlled and non-randomized study on 120 patients with advanced PCa after HT who received HIFU, CRT, HIFU＋LRT and HT alone, respectively. CT/MR imaging showed the primary tumours and pelvic lymph node metastases visibly shrank or even disappeared after HIFU ＋LRT treatment. There were significant differences among four groups with regard to overall survival （OS） and disease-specific survival （DSS） curves （P=0.018 and 0.015）. Further comparison between each pair of groups suggested that the long-term DSS of the H IFU ＋ LRT group was higher than those of the other three groups, but there was no significant difference between the HIFU＋LRT group and the CRT group. Multivariable Cox＇s proportional hazard model showed that both HIFU＋LRT and CRT were independently associated with DSS （P=0.001 and 0.035） and had protective effects with regard to the risk of death. Compared with CRT, HIFU ＋LRT significantly decreased incidences of radiation-related late gastrointestinal （GI） and genitourinary （GU） toxicity grade ≥ II. In conclusion, long-term survival of patients with advanced PCa benefited from strengthening local control of primary tumour and reRional lymph node metastases after HT. As an alternative to CRT, HIFU＋LRT showed Rood efficacy and better safety.

Mutation Breeding of an Organic Phosphorus-Solubilizing Bacterium B3 by Low Energy Ion Beam Implantation

A preliminary study on the mutation breeding of an organic phosphorus-solubilizing bacterium B3 by low-energy N^＋ ion beam was conducted. Analysis of dosage vs. survival rate and mutation rate showed that the dosage of 3×10^15 ions/cm^2 was optimal for the mutation. Some parameters for determining the efficient phosphorus content were studied to simplify the protocols for screening. Ultimately one reliable mutant was screened out under plating and flask-culturing screening conditions. Importantly, a novel phosphorus-dissolving mechanism is reported for the first time in terms of bio-surfactant production, which indicates that bio-surfactant might play a vital role in phosphorus-dissolving for some phosphobacteria.

Evidence for Base Substitutions and Repair of DNA Mismatch Damage Induced by Low Energy N^+ Ion Beam Implantation in E. coli

Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.

Low Velocity Impact Response Analysis of Shape Memory Alloy Reinforced Composite Beam

The low velocity impact responses of shape memory alloy （ SMA ） reinforced composite beams were analyzed by employing the finite element method. The finite element dynamic equntion was solved by the Newmark direct integration method, the impact contact force was determined asing the Hertzian contact law, and the influence of SMA .fibers on stiffiwss matrix is studied. Numerical results show that the SMA fibers can effectively improve the low velocity impact response property of composite beam.

Analysis of a Partial Male-Sterile Mutant of Arabidopsis thaliana Isolated from a Low-Energy Argon Ion Beam Mutagenized Pool

A screen for Arabidopsis fertility mutants, mutagenized by low-energy argon ion beam, yielded two partial male-sterile mutants tc243-1 and tc243-2 which have similar phenotypes. tc243-2 was investigated in detail. The segregation ratio of the mutant phenotypes in the M2 pools suggested that mutation behaved as single Mendelian recessive mutations, tc243 showed a series of mutant phenotypes, among which partial male-sterile was its striking mutant characteristic. Phenotype analysis indicates that there are four factors leading to male sterility, a. Floral organs normally develop inside the closed bud, but the anther filaments do not elongate sufficiently to position the locules above the stigma at anthesis, b. The anther locules do not dehisce at the time of flower opening （although limited dehiscence occurs later）, c. Pollens of mutant plants develop into several types of pollens at the trinucleated stage. as determined by staining with DAPI （4＇,6-diamidino-2-phenylindole）. which shows a variable size. shape and number of nucleus. d. The viability of pollens is lower than that of the wild type on the germination test in vivo and vitro.

Beam Characteristics at Low Dose Monitor Unit Settings for Vero4DRT

The stability of delivery of low monitor unit (MU) setting is important especially for step-and-shoot intensity-modulated radiotherapy (IMRT), because the nature of the technique is inherent to repeat beam on/off according to the number of the segments. This study evaluates the dose linearity and profile flatness/symmetry under low MU settings for Vero4DRT, a new linear-accelerator based irradiation system that currently implements step-and-shoot IMRT. To evaluate the dose linearity and flatness/symmetry, the point doses and beam profiles were measured as functions of MU and dose rates. The accuracy of dose delivery depended on the dose rate. Under all dose rates, the dose was linear within 1% above 5 MU and within 2% above 3 MU. The beam symmetry was degraded in-line compared with crossline, although both profiles were symmetric within 2% at all dose settings. The profile flatness was also within 2% above 5 MU at any dose rate and showed no significant variation among the low MU settings. To ensure stable beam delivery without increasing the treatment time of Vero4DRT, we recommend a delivery of 5 MU per segment at a dose rate of 500 MU/min.

Slice-wise reconstruction for low-dose cone-beam CT using a deep residual convolutional neural network

Because of the growing concern over the radiation dose delivered to patients, X-ray cone-beam CT(CBCT) imaging of low dose is of great interest. It is difficult for traditional reconstruction methods such as Feldkamp to reduce noise and keep resolution at low doses. A typical method to solve this problem is using optimizationbased methods with careful modeling of physics and additional constraints. However, it is computationally expensive and very time-consuming to reach an optimal solution. Recently, some pioneering work applying deep neural networks had some success in characterizing and removing artifacts from a low-dose data set. In this study,we incorporate imaging physics for a cone-beam CT into a residual convolutional neural network and propose a new end-to-end deep learning-based method for slice-wise reconstruction. By transferring 3D projection to a 2D problem with a noise reduction property, we can not only obtain reconstructions of high image quality, but also lower the computational complexity. The proposed network is composed of three serially connected sub-networks: a cone-to-fan transformation sub-network, a 2D analytical inversion sub-network, and an image refinement sub-network. This provides a comprehensive solution for end-to-end reconstruction for CBCT. The advantages of our method are that the network can simplify a 3D reconstruction problem to a 2D slice-wise reconstruction problem and can complete reconstruction in an end-to-end manner with the system matrix integrated into the network design. Furthermore, reconstruction can be less computationally expensive and easily parallelizable compared with iterative reconstruction methods.

A New Synthesis Method for Sum and Difference Beam Pattern with Low Sidelobe

A new method for sum and difference beam pattern synthesis with low sidelobe is proposed.In the situation of phase taper weighting,it makes use of Fourier transform pair relationship existing between the array factor and the element excitations and uses a loop iterative way to derive the array element excitations according to given array factor.The iteration does not stop until the array element excitations meet the initial sidelobe requirements.The method overcomes the defects of traditional methods,such as more searching times,larger amount of calculation and poor robustness.In addition,it can also synthesize the low sidelobe pattern in case of element failures.Simulation verifies the effectiveness of the method.

迈腾B8前照灯结构及故障诊断逻辑分析

介绍了迈腾B8灯光系统的主要组成:灯具、开关、保险、接地、继电器、控制单元。进而介绍迈腾B8前大灯的组成及前照灯的灯光控制系统组成。归纳、绘制了迈腾B8的前大灯电路控制原理图,表述了相关的控制单元、用电设备、开关、继电器的关联。在迈腾B8的远光电路控制原理基础上,解释了LED前大灯的工作机理及近光、远光的电流路径。基于电流形成路径,总结了故障诊断时的电压、电阻检测条件、方法与结果。

基于异形坯钢种低成本脱氧的实践与研究

基于现场实际生产模型,结合工业工程手法,对异形坯生产过程中的脱氧剂进行研究。发现生产过程中降低出钢氧,同时调整脱氧剂结构,能够有效降低脱氧剂成本,实现低成本脱氧的目的。

近光灯八点配光设计

基于GB、ECE和FMVSS三个标准体系中近光灯照明区域关于Ⅲ区内八点照度的配光要求,研究通过优化近光透镜(PES)模组结构实现八点照度配光设计,论述了两种光学方案及其工作原理。采用光线追踪的方式,详细分析其结构特征、质量保证,并模拟其配光结果,对比两种方案优缺点,为灯具设计提供理论依据和设计方案。

高黏橡胶改性沥青制备及其流变性能研究

排水路面是促进可持续发展和建设海绵城市的有效方法之一,该文研究适用于排水沥青路面的高黏改性沥青,采用主要成分为SBS颗粒和高黏橡胶颗粒对基质沥青进行改性,通过正交试验,分别采用60℃动力黏度、软化点、针入度、延度、弹性恢复指标确定高黏橡胶改性沥青的最佳掺量。选择常用的TPS改性剂和XC改性剂作为对照组进行对比分析,利用弯曲梁流变试验(BBR)和动态剪切流变试验(DSR)对三种高黏沥青的低温抗裂性和高温稳定性进行分析和评价,结果表明根据正交试验获得的最佳配方所制得的高黏橡胶改性沥青性能优良,为高黏改性沥青在道路工程领域中的开发和应用提供有价值的参考。

低预应力CFRP板加固RC梁模型试验研究

为研究低预应力CFRP加固RC梁的力学性能,设计进行了CFRP加固方法的对比模型梁试验。通过对直接粘贴、机械张拉粘贴锚固成为低应力CFRP板及粘贴加热伸长后回缩成为低预应力CFRP板这3种方法,将同规格的CFRP板粘贴在混凝土试验梁表面,通过分级加载试验,获得不同施工方法的CFRP板加固后开裂荷载、极限荷载、裂缝发展及荷载-挠度曲线等。试验结果表明:3种施工方法下进行机械张拉低预应力CFRP板加固对原梁抗剪承载力性能提高较大。在正常使用阶段,机械张拉低预应力CFRP板加固梁和加热张拉低预应力CFRP板加固梁出现斜裂缝的荷载等级高于直接粘贴CFRP加固梁出现斜裂缝的荷载等级。机械张拉低预应力CFRP板加固梁和加热张拉低预应力CFRP板加固梁产生的裂缝宽度小于直接粘贴CFRP板加固梁产生的裂缝宽度。相对最明显的是机械张拉低预应力CFRP板,裂缝一直维持在较低水平。粘贴锚固的低预应力CFRP加固方式操作相对简单,可应用于桥梁维修或拆除前临时加固工程等。

高增益低旁瓣宽扇形波束脊形波导缝隙天线阵列设计

提出了一种具有高增益低副瓣的脊形波导缝隙阵列天线,中心工作频率为24.125 GHz,其包括一个八路馈电网络和一个尺寸为400 mm×65 mm的8×40辐射缝隙。通过波束合成方法提取天线阵列的期望激励分布,采用截止模式功率分配器灵活控制功率比。使用三维电磁仿真软件HFSS综合仿真计算,在中心频率处,获得仰角平面上的旁瓣电平(sidelobe level,SLL)和半功率波束宽度(half power beam width,HPBW)分别为-20.9 dB和54.5°、方位角平面上的SLL和HPBW分别为-27.8 dB和2.5°、峰值增益为23.2 dBi,仿真结果与理论分析一致。此天线可以同时实现低旁瓣的宽扇形波束,覆盖较宽的检测范围,并减轻来自其他方向的干扰,具有应用于空中探测、反无人机、气象雷达和成像雷达的潜力。