In an attempt to understand the effects of high energy shock wave (HESW)on renal function, we studied prospectively 40 patients with nephrolithiasis in 4 groups,using same voltage with different numbers of shock wave ...In an attempt to understand the effects of high energy shock wave (HESW)on renal function, we studied prospectively 40 patients with nephrolithiasis in 4 groups,using same voltage with different numbers of shock wave therapy to identify the difference of effects on renal function. Stone burdens and posit ion were similar in these groups. Each group received 1500, 2000, 2500 or 3000 puises at 12. 5 KV on JT-3lithotripotor respectively. All the groups had significantly increased the levels of urinary NAG, β2MG, ALB and serum β2MG, which reached the highest values on 1-3days after ESWL (P<0. 001), and then decreased to the pre-ESWL levels except urinary NAG in group Cand D and serum β2MG which were still significantly higher (P<0.05) than those before-ESWL on the 7th day after ESWL. There was significant correlation between either urinary NAG (γ=0. 977, P<0. 05) or β2MG (γ=0. 933, P<0. 001) with the number of shock wave. In addition, urinary NAG and β2MG increased significantly when the number of shock waves was over 2500 shots.These above findings suggest that shock wave had induced acute changes in renal functions and transient renal tubular damages, although these functional changes recovered within one week, and the tubular damage might last longer than 7 days , In order to avoid serious renal damage, it’s necessary to limit the energy level of shock waves under 12. 5 KV×2500 shots by using JT-3 lithotriptor.展开更多
Stem cell transplantation and low-energy shock-wave therapy （LESWT） have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in di...Stem cell transplantation and low-energy shock-wave therapy （LESWT） have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups： the diabetic group （n = 6）, the LESWT group （n = 6）, the bone marrow-derived mesenchymal stem cell （BMSC） transplantation group （n = 15）, and the combination of LESWT and BMSC transplantation group （n = 15）. One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed： the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.展开更多
体外冲击波碎石(ESWL)是一种无创治疗泌尿系结石的医疗技术,临床上有95%以上的成功率,冲击波能量是这种医疗技术成功的关键因素。为此,阐述了冲击波的形成、传播、聚焦,冲击波发生器的电场能量和碎石原理等,然后从ESWL成功率密度分布中...体外冲击波碎石(ESWL)是一种无创治疗泌尿系结石的医疗技术,临床上有95%以上的成功率,冲击波能量是这种医疗技术成功的关键因素。为此,阐述了冲击波的形成、传播、聚焦,冲击波发生器的电场能量和碎石原理等,然后从ESWL成功率密度分布中分析冲击波最佳能量。ESWL病例入选条件是门诊输尿管结石患者,年龄在18~83岁之间,左、右单侧或双侧输尿管具有放射影像学结石特征,ESWL数据完整,并且输尿管结石ESWL成功者。通过建立能量的ESWL成功率密度分布函数,得到其数学期望和均方差,从而获得ESWL成功的最佳能量。研究结果表明:从入选的5 747例ESWL病例中,获得ESWL成功率密度分布函数的数学期望为535 m J/mm^2,均方差为157 m J/mm^2,最佳能量在418~732 m J/mm^2范围内;该最佳能量可以通过能级来确定,能级一般分为Ⅰ级(约10 m J/mm^2)、Ⅱ级(20~40 m J/mm^2)、Ⅲ级(30~300 m J/mm^2)、Ⅳ级(400~1 200 m J/mm^2);可以根据结石的大小和密度来选择能级。该结果有助于临床医师根据实际情况选择能量,以提高ESWL成功率。展开更多
目的观察低能量体外冲击波(ESW)对膝骨关节炎大鼠软骨细胞凋亡的影响。方法将30只裸大鼠按随机数字表法分为空白组(n=10)、模型组(n=10)、低能量ESW组(n=10);模型组与低能量ESW组均建立KOA模型,空白组不予以建模;建模6周后,拆除石膏,低...目的观察低能量体外冲击波(ESW)对膝骨关节炎大鼠软骨细胞凋亡的影响。方法将30只裸大鼠按随机数字表法分为空白组(n=10)、模型组(n=10)、低能量ESW组(n=10);模型组与低能量ESW组均建立KOA模型,空白组不予以建模;建模6周后,拆除石膏,低能量ESW组予以低能量(0.1 m J/mm2)ESW治疗,冲击1000次。4周后,处死大鼠,行组织形态大体观察及染色观察,并采用TENUL方法对软骨细胞凋亡情况进行检测。结果干预4周末,空白组软骨外观显光亮蓝白色,无裂痕、缺损,软骨结构、潮线显示清楚;模型组膝关节呈现典型的KOA病理改变;而低能量ESW组软骨触摸有软化,少许脱落,潮线模糊。低能量ESW组Moran评分为(5.93±0.71)分,明显高于模型组的(4.32±0.76)分(P<0.05);低能量ESW组Mankin评分、细胞凋亡率分别为(8.03±1.07)分、(12.12±1.59)%,均明显低于模型组的(9.78±1.76)分、(14.25±2.23)%,差异有统计学意义(P<0.05)。结论低能量ESW可有效抑制KOA大鼠软骨细胞凋亡,对KOA治疗有重要参考价值。展开更多
文摘In an attempt to understand the effects of high energy shock wave (HESW)on renal function, we studied prospectively 40 patients with nephrolithiasis in 4 groups,using same voltage with different numbers of shock wave therapy to identify the difference of effects on renal function. Stone burdens and posit ion were similar in these groups. Each group received 1500, 2000, 2500 or 3000 puises at 12. 5 KV on JT-3lithotripotor respectively. All the groups had significantly increased the levels of urinary NAG, β2MG, ALB and serum β2MG, which reached the highest values on 1-3days after ESWL (P<0. 001), and then decreased to the pre-ESWL levels except urinary NAG in group Cand D and serum β2MG which were still significantly higher (P<0.05) than those before-ESWL on the 7th day after ESWL. There was significant correlation between either urinary NAG (γ=0. 977, P<0. 05) or β2MG (γ=0. 933, P<0. 001) with the number of shock wave. In addition, urinary NAG and β2MG increased significantly when the number of shock waves was over 2500 shots.These above findings suggest that shock wave had induced acute changes in renal functions and transient renal tubular damages, although these functional changes recovered within one week, and the tubular damage might last longer than 7 days , In order to avoid serious renal damage, it’s necessary to limit the energy level of shock waves under 12. 5 KV×2500 shots by using JT-3 lithotriptor.
文摘Stem cell transplantation and low-energy shock-wave therapy （LESWT） have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups： the diabetic group （n = 6）, the LESWT group （n = 6）, the bone marrow-derived mesenchymal stem cell （BMSC） transplantation group （n = 15）, and the combination of LESWT and BMSC transplantation group （n = 15）. One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed： the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.
文摘体外冲击波碎石(ESWL)是一种无创治疗泌尿系结石的医疗技术,临床上有95%以上的成功率,冲击波能量是这种医疗技术成功的关键因素。为此,阐述了冲击波的形成、传播、聚焦,冲击波发生器的电场能量和碎石原理等,然后从ESWL成功率密度分布中分析冲击波最佳能量。ESWL病例入选条件是门诊输尿管结石患者,年龄在18~83岁之间,左、右单侧或双侧输尿管具有放射影像学结石特征,ESWL数据完整,并且输尿管结石ESWL成功者。通过建立能量的ESWL成功率密度分布函数,得到其数学期望和均方差,从而获得ESWL成功的最佳能量。研究结果表明:从入选的5 747例ESWL病例中,获得ESWL成功率密度分布函数的数学期望为535 m J/mm^2,均方差为157 m J/mm^2,最佳能量在418~732 m J/mm^2范围内;该最佳能量可以通过能级来确定,能级一般分为Ⅰ级(约10 m J/mm^2)、Ⅱ级(20~40 m J/mm^2)、Ⅲ级(30~300 m J/mm^2)、Ⅳ级(400~1 200 m J/mm^2);可以根据结石的大小和密度来选择能级。该结果有助于临床医师根据实际情况选择能量,以提高ESWL成功率。
文摘目的观察低能量体外冲击波(ESW)对膝骨关节炎大鼠软骨细胞凋亡的影响。方法将30只裸大鼠按随机数字表法分为空白组(n=10)、模型组(n=10)、低能量ESW组(n=10);模型组与低能量ESW组均建立KOA模型,空白组不予以建模;建模6周后,拆除石膏,低能量ESW组予以低能量(0.1 m J/mm2)ESW治疗,冲击1000次。4周后,处死大鼠,行组织形态大体观察及染色观察,并采用TENUL方法对软骨细胞凋亡情况进行检测。结果干预4周末,空白组软骨外观显光亮蓝白色,无裂痕、缺损,软骨结构、潮线显示清楚;模型组膝关节呈现典型的KOA病理改变;而低能量ESW组软骨触摸有软化,少许脱落,潮线模糊。低能量ESW组Moran评分为(5.93±0.71)分,明显高于模型组的(4.32±0.76)分(P<0.05);低能量ESW组Mankin评分、细胞凋亡率分别为(8.03±1.07)分、(12.12±1.59)%,均明显低于模型组的(9.78±1.76)分、(14.25±2.23)%,差异有统计学意义(P<0.05)。结论低能量ESW可有效抑制KOA大鼠软骨细胞凋亡,对KOA治疗有重要参考价值。
