Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal d...Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.展开更多
目的探讨低频脉冲治疗仪结合手法按摩对缺乳初产妇母乳喂养情况的影响。方法选取沂南县人民医院2020年10月至2022年10月收治的112例缺乳初产妇为研究对象,采用随机数字表法分为对照组和研究组,各56例。对照组采用低频脉冲治疗仪干预,研...目的探讨低频脉冲治疗仪结合手法按摩对缺乳初产妇母乳喂养情况的影响。方法选取沂南县人民医院2020年10月至2022年10月收治的112例缺乳初产妇为研究对象,采用随机数字表法分为对照组和研究组,各56例。对照组采用低频脉冲治疗仪干预,研究组采用低频脉冲治疗仪结合手法按摩干预,比较两组乳房胀痛程度(VAS)、母乳喂养状况(MBFES)及母乳喂养成功率。结果研究组产后3、4 d VAS评分低于对照组(P<0.05);两组组内不同时间点VAS评分比较,差异有统计学意义(P<0.05)。研究组干预后MBFES评分高于对照组(P<0.05);研究组母乳喂养成功率高于对照组(P<0.05)。结论低频脉冲治疗仪结合手法按摩可以缓解产妇乳房胀痛,改善母乳喂养状况,提高母乳喂养成功率。展开更多
基金supported by a grant of the Korea Health Technology R & D Project through the Korea Health Industry Development Institute(KHIDI)funded by the Ministry of Health&Welfare,Republic of Korea(grant number:HI15C1535)
文摘Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.
文摘目的探讨低频脉冲治疗仪结合手法按摩对缺乳初产妇母乳喂养情况的影响。方法选取沂南县人民医院2020年10月至2022年10月收治的112例缺乳初产妇为研究对象,采用随机数字表法分为对照组和研究组,各56例。对照组采用低频脉冲治疗仪干预,研究组采用低频脉冲治疗仪结合手法按摩干预,比较两组乳房胀痛程度(VAS)、母乳喂养状况(MBFES)及母乳喂养成功率。结果研究组产后3、4 d VAS评分低于对照组(P<0.05);两组组内不同时间点VAS评分比较,差异有统计学意义(P<0.05)。研究组干预后MBFES评分高于对照组(P<0.05);研究组母乳喂养成功率高于对照组(P<0.05)。结论低频脉冲治疗仪结合手法按摩可以缓解产妇乳房胀痛,改善母乳喂养状况,提高母乳喂养成功率。
