To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars(W1240, W1721, W025) and an ordinary rice ...To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars(W1240, W1721, W025) and an ordinary rice cultivar(H9405) with five N treatments were carried out to determine the effects of N application rate and genotype on protein fractions contents and Glutelin/Prolamin ratio(Glu/Pro). The difference of protein fraction concentrations affected by N application rate existed in genotypes. Ordinary rice cultivar had a larger increase in glutlein concentration affected by N application rate than low-glutelin rice cultivars did. Glutelin in H9405 had a increase of 30.6 and 41.0% under the N4 treatment(360 kg N ha^(–1)) when compared with N0 treatment(no fertilizer N) in 2010 and 2011 respectively, while all the low-glutelin rice cultivars showed relatively smaller increases for two years. Variance analysis showed no significant effect of N application rate on glutelin in W1240 and W025 while the effects on albumin, globulin and prolamin were significant in low-glutelin rice. What's more, N treatment had no significant i nfluence on Glu/Pro ratios in low-glutelin rice cultivars while a significant increase in Glu/Pro ratio was observed in ordinary rice cultivar. So low-gultelin rice cultivars showed a different pattern from ordinary rice cultivars when influenced by N application rate.展开更多
To investigate the effect of low light (LL, 50% natural light) during grain filling (GF) stage on rice transamination, amino acid (AA) accumulation, nutritional value, and cooking quality in three different rice...To investigate the effect of low light (LL, 50% natural light) during grain filling (GF) stage on rice transamination, amino acid (AA) accumulation, nutritional value, and cooking quality in three different rice genotypes, transaminase activities and AA levels in grains during GF stage and the traits that significantly affected rice quality (physical appearance, cooking quality, and nutritional value) were analyzed. LL did not disturb transamination in rice grains during GF stage, as minimal impact was found on alanine and aspartate transaminase activities. Nevertheless, most AAs in caryopses, including lysine and threonine, increased in response to LL, except for sulfur-containing AAs. These results suggest that AA metabolism and accumulation in rice grains were rarely suppressed by LL during GF stage. Rice nutritional ingredients at harvest, such as major protein components including glutelin and most important essential amino acids (EAAs) including lysine and threonine, increased significantly in response to LL, whereas most protein and EAA ratios were rarely affected. However, LL markedly affected physical appearance of rice grains by reducing brown rice rate, milled rice rate, and 1000-grain weight and increasing the chalkiness rate. In addition, cooking qualities decreased in response to LL, while breakdown values and amylose levels decreased and setback values increased. We concluded that LL during GF stage decreased the cooking quality of rice, but could potentially improve the nutritional value of rice.展开更多
慢性肾脏病(chronic kidney disease,CKD)发病率在我国呈逐年上升趋势,低蛋白饮食(low protein diet,LPD)是延缓CKD病情发展有效的食疗方法之一。本文就近年来日益受到广泛关注的低蛋白调整大米的研究方法进行了综述和展望,分析了包括...慢性肾脏病(chronic kidney disease,CKD)发病率在我国呈逐年上升趋势,低蛋白饮食(low protein diet,LPD)是延缓CKD病情发展有效的食疗方法之一。本文就近年来日益受到广泛关注的低蛋白调整大米的研究方法进行了综述和展望,分析了包括育种栽培、物理方法、化学方法、生物技术降解等方法的优势与不足。其中利用生物技术降解整粒大米蛋白,能够在保持大米原有完整性和口感的同时大幅度降低其蛋白含量,产品更适合肾病患者长期食用。我国约2/3居民以大米为主食,肾病患者人数越来越多,而国内相关的低蛋白产品还相对较少,无法大众化,故从改善患者主食方面研制出符合患者饮食习惯的低蛋白大米的市场前景非常可观。展开更多
基金provided by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B14, 2012BAD20B05, 2012BAD04B08)
文摘To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars(W1240, W1721, W025) and an ordinary rice cultivar(H9405) with five N treatments were carried out to determine the effects of N application rate and genotype on protein fractions contents and Glutelin/Prolamin ratio(Glu/Pro). The difference of protein fraction concentrations affected by N application rate existed in genotypes. Ordinary rice cultivar had a larger increase in glutlein concentration affected by N application rate than low-glutelin rice cultivars did. Glutelin in H9405 had a increase of 30.6 and 41.0% under the N4 treatment(360 kg N ha^(–1)) when compared with N0 treatment(no fertilizer N) in 2010 and 2011 respectively, while all the low-glutelin rice cultivars showed relatively smaller increases for two years. Variance analysis showed no significant effect of N application rate on glutelin in W1240 and W025 while the effects on albumin, globulin and prolamin were significant in low-glutelin rice. What's more, N treatment had no significant i nfluence on Glu/Pro ratios in low-glutelin rice cultivars while a significant increase in Glu/Pro ratio was observed in ordinary rice cultivar. So low-gultelin rice cultivars showed a different pattern from ordinary rice cultivars when influenced by N application rate.
文摘To investigate the effect of low light (LL, 50% natural light) during grain filling (GF) stage on rice transamination, amino acid (AA) accumulation, nutritional value, and cooking quality in three different rice genotypes, transaminase activities and AA levels in grains during GF stage and the traits that significantly affected rice quality (physical appearance, cooking quality, and nutritional value) were analyzed. LL did not disturb transamination in rice grains during GF stage, as minimal impact was found on alanine and aspartate transaminase activities. Nevertheless, most AAs in caryopses, including lysine and threonine, increased in response to LL, except for sulfur-containing AAs. These results suggest that AA metabolism and accumulation in rice grains were rarely suppressed by LL during GF stage. Rice nutritional ingredients at harvest, such as major protein components including glutelin and most important essential amino acids (EAAs) including lysine and threonine, increased significantly in response to LL, whereas most protein and EAA ratios were rarely affected. However, LL markedly affected physical appearance of rice grains by reducing brown rice rate, milled rice rate, and 1000-grain weight and increasing the chalkiness rate. In addition, cooking qualities decreased in response to LL, while breakdown values and amylose levels decreased and setback values increased. We concluded that LL during GF stage decreased the cooking quality of rice, but could potentially improve the nutritional value of rice.
文摘采用挤压工艺制备低蛋白重组米,以崩解值为评价指标,以机筒温度、物料含水率、螺杆转速为考察因素,通过试验得出最佳工艺参数为机筒温度102℃、物料含水率30%、螺杆转速132 r/min,此条件下得到的低蛋白重组米崩解值达到最大值(1 113.9 c P),外观和颜色上非常接近粳米。初步的饮食干预试验可得,喂食低蛋白重组米的肾损伤大鼠体重高于标准饮食和正常大米组,且低蛋白重组米组大鼠的血肌酐、尿素氮、白蛋白水平与标准饮食和正常大米组相比差异显著(Ρ<0.05),表明低蛋白重组米由于含有的蛋白质含量较少,对肾损伤大鼠的肾脏具有一定的保护作用。
文摘慢性肾脏病(chronic kidney disease,CKD)发病率在我国呈逐年上升趋势,低蛋白饮食(low protein diet,LPD)是延缓CKD病情发展有效的食疗方法之一。本文就近年来日益受到广泛关注的低蛋白调整大米的研究方法进行了综述和展望,分析了包括育种栽培、物理方法、化学方法、生物技术降解等方法的优势与不足。其中利用生物技术降解整粒大米蛋白,能够在保持大米原有完整性和口感的同时大幅度降低其蛋白含量,产品更适合肾病患者长期食用。我国约2/3居民以大米为主食,肾病患者人数越来越多,而国内相关的低蛋白产品还相对较少,无法大众化,故从改善患者主食方面研制出符合患者饮食习惯的低蛋白大米的市场前景非常可观。