Changes in plasma calcitonin gene-related peptide and serum neuron specific enolase in rats with acute cerebral ischemia after low-frequency electrical stimulation with different waveforms and intensities

Following acute cerebral ischemia in rats, plasma calcitonin gene-related peptide decreased and the level of serum neuron specific enolase and the volume of the infarction increased. Square-wave and triangular-wave electrical stimulation with low or high intensities could increase the plasma calcitonin gene-related peptide, decrease the serum neuron specific enolase and reduce the infarction volume in the brain in rats with cerebral ischemia. There was no significant difference between different wave forms and intensities. The experimental findings indicate that low-frequency electrical stimulation with varying waveforms and intensities can treat acute cerebral ischemia in rats.

Pulsed electrical stimulation protects neurons in the dorsal root and anterior horn of the spinal cord after peripheral nerve injury

Most studies on peripheral nerve injury have focused on repair at the site of injury, but very few have examined the effects of repair strategies on the more proximal neuronal cell bodies. In this study, an approximately 10-mm-long nerve segment from the ischial tuberosity in the rat was transected and its proximal and distal ends were inverted and sutured. The spinal cord was subjected to pulsed electrical stimulation at T10 and L3, at a current of 6.5 m A and a stimulation frequency of 15 Hz, 15 minutes per session, twice a day for 56 days. After pulsed electrical stimulation, the number of neurons in the dorsal root ganglion and anterior horn was increased in rats with sciatic nerve injury. The number of myelinated nerve fibers was increased in the sciatic nerve. The ultrastructure of neurons in the dorsal root ganglion and spinal cord was noticeably improved. Conduction velocity of the sciatic nerve was also increased. These results show that pulsed electrical stimulation protects sensory neurons in the dorsal root ganglia as well as motor neurons in the anterior horn of the spinal cord after peripheral nerve injury, and that it promotes the regeneration of peripheral nerve fibers.

Effect of low-frequency pulse percutaneous electric stimulation on peripheral nerve injuries at different sites

BACKGROUND： The postoperative recovery of nerve function in patients with peripheral nerve injury is always an important problem to solve after treatment. The electric stimulation induced electromagnetic field can nourish nerve, postpone muscular atrophy, and help the postoperative neuromuscular function. OBJECTIVE： To observe the effects of low-frequency pulse percutaneous electric stimulation on the functional recovery of postoperative patients with peripheral nerve injury, and quantitatively evaluate the results of electromyogram （EMG） examination before and after treatment. DESIGN ： A retrospective case analysis SETTING： The Sixth People＇s Hospital affiliated to Shanghai Jiaotong University PARTICIPANTS： Nineteen postoperative inpatients with peripheral nerve injury were selected from the De- partment of Orthopaedics, the Sixth People＇s Hospital affiliated to Shanghai Jiaotong University from June 2005 to January 2006, including 13 males and 6 females aged 24-62 years with an average of 36 years old. There were 3 cases of brachial plexus nerve injury, 3 of median nerve injury, 7 of radial nerve injury, 3 of ul- nar nerve injury and 3 of common peroneal nerve injury, and all the patients received probing nerve fiber restoration. Their main preoperative manifestations were dennervation, pain in limbs, motor and sensory disturbances. All the 19 patients were informed with the therapeutic program and items for evaluation. METHODS： ① Low-frequency pulse percutaneous electric stimulation apparatus： The patients were given electric stimulation with the TERESA cantata instrument （TERESA-0, Shanghai Teresa Health Technology, Co., Ltd.）. The patients were stimulated with symmetric square waves of 1-111 Hz, and the intensity was 1.2-5.0 mA, and it was gradually adjusted according to the recovered conditions of neural regeneration following the principle that the intensity was strong enough and the patients felt no obvious upset. They were treated for 4- 24 weeks, 10-30 minutes for each time, 1-3 times a day, and 6 weeks as a course. ② EMG examination was applied to evaluate the recoveries of recruitment, motor conduction velocity （MCV） and sensory conduction velocity （SCV） before and after treatment. The patients were examined with the EMG apparatus （DIS- A2000C, Danmark） before and after the treatment of percutaneous electric stimulation. ③Standards for evaluating the effects included cured （complete recovery of motor functions, muscle strength of grade 5, no abnormality in EMG examination）, obviously effective [general recovery of motor function, muscle strength of grade 4, no or a few denervation potentials, motor conduction velocity （MCV） and sensory conduction velocity （SCV）], improved （partial recovery of motor function, muscle strength of grade 3, denervation potentials and reinneration potentials, slowed MCV and SCV, invalid （no obvious changes of motor function）. MAIN OUTCOME MEASURES： ① Ameliorated degree of the nerve function of the postoperative patients with peripheral nerve injury treated with percutaneous electric stimulation; ② Changes of EMG examination before and after treatment. RESULTS： All the 19 postoperative patients with peripheral nerve injury were involved in the analysis of results. ① Comparison of nerve function before and after treatment in 19 patients with peripheral nerve injury of different sites： For the patients with radial nerve injury （n=7）, the nerve functions all completely recovered after 8-week treatment, and the cured and obvious rate was 100% （7/7）; For the patients with brachial plexus nerve injury （n=3）, 1 case had no obvious improvement, and the cured and obvious rate was 67% （2/3）; For the patients with common peroneal nerve injury （n=3）, the extension of foot dorsum generally recovered in 1 case of nerve contusion after 4-week treatment, and the cured and obvious rate was 67% （2/3）; For the patients with median nerve injury （n=3）, muscle strength was obviously recovered, and the cured and obvious rate was 100% （3/3）; For the patients with ulnar nerve injury （n=3）, 1 case only had recovery of partial senses, and the cured and obvious rate was 67% （2/3）. Totally 9 cases were cured, 7 were obviously effective, 1 was improved, and only 2 were invalid. After 4 courses, the cured rate of damaged nerve function after four courses was 47% （9/19）, and effective rate was 89% （17/19）.② Comparison of EMG examination before and after treatment： Before and after percutaneous electric stimulation, he effective rates of recruitment, MCV and SCV were 89% （17/19）, 58% （11/19）, 47% （9/19） respectively, and there were extremely obvious differences （P〈 0.01）. CONCLUSION： ①Low-frequency pulse percutaneous electric stimulation can improve the nerve function of postoperative patients with peripheral nerve injury of different sites, especially that the injuries of radial nerve and median nerve recover more obviously. ②Percutaneous electric stimulation can ameliorate the indexes of EMG examination, especially the recruitment, in postoperative patients with peripheral nerve injury.

Effects of cervical low-frequency electrical stimulation with various waveforms and densities on body mass,liver and kidney function,and death rate in ischemic stroke rats

Low-frequency electrical stimulation has resulted in favorable effects in the treatment of post-stroke dysphagia. However, the safety of cervical low-frequency electrical stimulation remains unclear because of numerous nerves and blood vessels in the neck. In the present study, rats with ischemic stroke underwent low-frequency electrical stimulation, and systemic and local effects of electrical stimulation at different densities and waveforms were investigated. Electrical stimulation resulted in no significant effects on body mass, liver or kidney function, or mortality rate. In addition, no significant adverse reaction was observed, despite overly high intensity of low-frequency electrical stimulation, which induced laryngismus, results from the present study suggested that it is safe to stimulate the neck with a low-frequency electricity under certain intensities.

Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway

AIM: To investigate the effects of different parameters of gastric electrical stimulation (GES) on interstitial cells of Cajal (ICCs) and changes in the insulin-like growth factor 1 (IGF-1) signal pathway in streptozotocin-induced diabetic rats. METHODS: Male rats were randomized into control, diabetic (DM), diabetic with sham GES (DM + SGES), diabetic with GES1 (5.5 cpm, 100 ms, 4 mA) (DM + GES1), diabetic with GES2 (5.5 cpm, 300 ms, 4 mA) (DM + GES2) and diabetic with GES3 (5.5 cpm, 550 ms, 2 mA) (DM + GES3) groups. The expression levels of c-kit, M-SCF and IGF-1 receptors were evaluated in the gastric antrum using Western blot analysis. The distribution of ICCs was observed using immunolabeling for c-kit, while smooth muscle cells and IGF-1 receptors were identified using alpha-SMA and IGF-1R antibodies. Serum level of IGF-1 was tested using enzyme-linked immunosorbent assay. RESULTS: Gastric emptying was delayed in the DM group but improved in all GES groups, especially in the GES2 group. The expression levels of c-kit, M-SCF and IGF-1R were decreased in the DM group but increased in all GES groups. More ICCs (c-kit(+)) and smooth muscle cells (alpha-SMA(+)/IGF-1R(+)) were observed in all GES groups than in the DM group. The average level of IGF-1 in the DM group was markedly decreased, but it was up-regulated in all GES groups, especially in the GES2 group. CONCLUSION: The results suggest that long-pulse GES promotes the regeneration of ICCs. The IGF-1 signaling pathway might be involved in the mechanism underlying this process, which results in improved gastric emptying.

Treatment time influences the effects of a low-frequency pulsed electric field on synthesis of tyrosine hydroxylase and dopamine in PC12 cells

BACKGROUND： Electromagnetic radiation can influence dopamine （DA） synthesis in brain tissues or ceils, but electromagnetic frequencies, intensities, and radiation time can produce different effects. In addition, the signal pathway by which electromagnetic radiation influences DA synthesis remains controversial. OBJECTIVE： To determine tyrosine hydroxylase （TH） expression in PC12 cells and DA levels in cell culture media after different periods of low-frequency pulsed electric field （LF-PEF） stimulation, and to determine how LF-PEF signaling stimulates TH synthesis using inhibitors. DESIGN, TIME AND SETTING： A parallel, controlled, cell experiment was performed at the Laboratory of Cell Biology, School of Life Science, East China Normal University, between January and October 2006. MATERIALS： PC12 cells were purchased from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China. Nerve growth factor was purchased from PeproTech, USA. The protein kinase A inhibitor, H-89, and mitogen-activated protein kinase kinase inhibitor, U0126, were purchased from Sigma, USA. METHODS： （1） Following routine culture in Dulbecco＇s modified eagle medium, primary PC12 cells were stimulated under LF-PEF （pulse frequency 50.Hz, pulse width 20 μs, peak field strength 1 V/m） for 5, 10, 15, 20, and 30 minutes. （2） Inhibitors （H-89 or U0126, 1 μmol/L） were added 30 minutes before LF-PEF stimulation for 10 minutes. MAIN OUTCOME MEASURES： （1） TH expression was determined by Western blot in PC12 cells at 0.5, 1,2, 3, and 4 days after LF-PEF stimulation. Similarly, DA was measured by high-performance liquid chromatography in media at 2, 3, 4, or 5 days after LF-PEE （2） TH expression was detected 1 day after H-89 or U0126 treatment and LF-PEE RESULTS： （1） Short-term LF-PEF stimulation （5 and 10 minutes） increased TH expression and media DA levels after short-term culture （2 days） （P 〈 0.01）, but both parameters decreased with longer culture （3 4 days） （P 〈 0.01）. Long-term LF-PEF stimulation （15, 20, or 30 minutes） decreased TH and DA synthesis, followed by a rapid increase （P 〈 0.01）. （2） H89 could completely inhibit TH expression in PC12 cells stimulated by LF-PEF for 10 minutes, while the inhibition rate of U0126 was 53.2%. CONCLUSION： Short-term LF-PEF first promotes then inhibits, while long-term LF-PEF first inhibits then promotes, TH and DA synthesis. LF-PEF stimulation regulates TH expression primarily by activating protein kinase A to regulate DA synthesis.

Central Opioid Peptide-containing Neurons Mediates Therapeutic Effect of Short-pulse Gastric Electrical Stimulation on Dyspepsia-like Symptoms in Dogs

This study investigated whether the curative effect of short-pulse gastric electrical stimulation （GES） on the vasopressin-induced dyspeptic symptoms was mediated by central opioid peptide-producing neurons. Five female beagle dogs implanted with 1 pair of electrodes in gastric serosa were used in a two-experiment study. In experiment one, the brain was scanned by positron emission tomography in 3 dogs with and without short-pulse GES, and the radioactivity in nuclei of solitary tract （NST） and hypothalamus was detected. Experiment two was composed of 4 sessions. In session one, the dogs were injected with vasopressin in the absence of short-pulse GES. With session two, the short-pulse GES was simultaneously given via the electrodes with the injection of vasopressin. In sessions three and four, naloxone and naloxone methiodide was administered respectively in the presence of short-pulse GES. Motion sickness-like symptoms were scored and compared among the different sessions. The results showed that the short-pulse GES significantly increased the radioactivity in NST and hypothalamic nuclei （P〈0.05, vs control）. The short-pulse GES could ameliorate the vasopressin-induced motion sickness-like symptoms in dogs. Naloxone, but not naloxone methiodide could attenuate the curative effects of short-pulse GES. It is concluded that NST and hypothalamic nuclei may participate in the mediation of the curative effects of short-pulse GES on dyspepsia-like symptoms. Central opioid peptide-containing neurons presumably mediate the therapeutic effect on dyspeptic symptoms of short-pulse GES.

Effect of Unilateral Low-Frequency Stimulation of Hippocampus on Rapid Kindling—Induced Seizure Development in Rats

Since the last decade deep brain stimulation has been proposed as an alternative treatment for patients who do not become seizure-free with the current pharmacological treatments and cannot undergo resective surgical procedure. However, the optimal stimulation parameters remain undetermined and active research in humans and animals is necessary. The present study was designed to investigate the effect of unilateral Low Frequency Stimulation (LFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK;n = 6) received only RK stimulus, while the treated group (LFS-hRK;n = 8) received also LFS (biphasic square wave pulses, 1 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing on day 3, 62% (P < 0.05) of the animals receiving LFS treatment were still not fully kindled staying in stages 0-III (P < 0.01). The number of stimulations needed to achieve generalized seizures (stage IV-V of Racine scale) was significantly higher (P < 0.05) in the LFS group with respect to control group. No significant differences in the cumulative daily afterdischarge duration were observed between both groups. These findings suggest that preemptive LFS can significantly decrease the incidence of hippocampus-kindled seizures and delay the progression and secondary generalization of focal seizures.

QUANTITATIVE ANALYSIS ON CHANGES OF HEPATIC HEMODYNAMICS AFTER TRANSCUTANEOUS ELECTRIC PULSE STIMULATION OF LOCAL POINTS BY USING COLOR DOPPLER ULTRASOUND DIAGNOSTIC APPARATUS

Objective: To evaluate the effect of transcutaneous electric pulse stimulation (TEPS) on hepatic blood flow and parenchymal microcirculation in patients with fatty liver. Methods: A total of 31 fatty liver volunteer patients were observed in this study. Changes of color Doppler energy (CDE) images before and after TEPS of local points nearby the liver were recorded by using color Doppler ultrasound diagnostic apparatus (ACUSON 128XP/10C). Sum of color pixel area (SCPA), average of color value (ACV) and SCPA×ACV (integral) of the hepatic flow images were analyzed by an image processing system, single blind method and paired t-test. Programmed TEPS (0.5- 150 Hz / 2 000 Hz , 10- 25 V ) was applied to the right Qimen (期门 LR 14)-Jingmen (京门 GB 25), Fuai (腹哀 SP 16)-Ganshu (肝俞 BL 18) respectively for 15 min. Results: Compared with basic values of pretreatment, SCPA, ACV and SCPA×ACV increased significantly (t=2.71, P<0.02; t=3.42, P<0.01; and t=8.15, P<0.001) after TEPS, meaning improvement of hepatic blood flow supply. Conclusion: TEPS of acupoints near the liver can improve hepatic blood flow and hepatic parenchymal microcirculation in patients with fatty liver.

Vegetative Development of <i>Sparassis crispa</i>in Various Growth Conditions and Effect of Electric Pulse Simulation on Its Fruit Body Production

This study was carried out to detect the favorable mycelial growth conditions and effect of electric pulse stimulation on fruit body production of SC-1 strain of Sparassis crispa. The optimum growth in PDA was found at 25°C (67 mm colony diameter in 28 days) followed by 20°C (63 mm colony diameter in 28 days). There was no mycelial growth at 35°C. The most favourable initial media pH range was found to be 5 - 7 and colony diameter measurements were not statistically different among these pH values (p > 0.05). However, the highest growth was obtained at pH 6 (57 mm colony diameter in 28 days). A basal medium composed of 0.05 g MgSO4, 0.46 g KH2PO4, 1.0 g K2HPO4, 120 μg thiamine-HCl, 20 g agar and 1000 ml of distilled water was used to investigate growth response of S. crispato different carbon and nitrogen sources. In 28 days, fructose and glucose exhibited best growth (49.4 mm and 31.6 mm colony diameters respectively) and there was no growth on the basal medium supplemented with galactose. Basal medium supplemented with glycine and alanine as nitrogen sources resulted in best growth, 54.4 mm and 50.5 mm colony diameters respectively. There was no mycelial growth in culture medium supplemented with ammonium acetate, ammonium phosphate, arginine and histidine. Electric pulse stimulation improved the fruiting body production. The yields obtained from all bottles in which electric pulse was applied were significantly higher than the yields from the bottles in which electric pulse was not applied. The percent increases of fresh weight yield from control on 100, 120, 130, and 170 kilovolts were 36%, 44%, 75% and 81% respectively. As regard to dry weight yield, the percent increases from control on 100, 120, 130, and 170 kilovolts were 27%, 54%, 63% and 67% respectively.

Calcium Phosphate Coating on Al_2O_3 Ceramics by a Biomimetic Method Using Electric Pulse Technique

The preparation of calcium phosphate （CP） coating on alumina ceramics using electric pulse stimulating methoe has been investigated. The cup-shaped alumina ceramics were soaked in a simulated body fluid （SBF）, and a square pulse potential with frequency of 1 Hz and voltage of 110 V was applied between the inner and outer surfaces of the alumina cup. Surface morphology of CP coatings during different deposition periods was observed by a Philips XL-30 scanning electron microscope （SEM）. Compositional analysis was examined by EDAX. The mechanism of nucleation and growth of CP coating was discussed. SEM result indicates that the coating comprises of a large number of tiny needle-like grains and has a porous microstructure. There is a strong bond between the deposited layer and Al2O3 substrate, which may be due to the gentle growth of the biomimetic method. The EDAX analysis indicates that main composition of the coating is calcium and phosphor. The formation of CP coating may be contributed to the stimulation of electric pulse and the high ions concentration which is 1.5 times of the concentration of SBF solution （1.5SBF solution）. Such surface functionalization method by electric pulse potential can be used to prepare CP coating on various electric-insulating bioinert materials for improving their bioactive character.

Hippocampal High-Frequency Stimulation Inhibites the Progression of Rapid Kindling-Induced Seizure in Rats

Epilepsy is one of the most common serious neurological disorders. Pharmacoresistant epilepsy patients are poorly controlled or their seizures are refractory to drug treatment. Resective surgery is frequently a promising therapy in this population, however, not all the patients meet the eligibility criteria for the surgical treatment. Deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation parameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimulation (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by our group. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK;n = 7) received only RK stimulus, while the treated group (HFS-hRK;n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 -III (p 0.01). HFS group needed a higher number of stimulations to achieve stage III (p 0.05) with respect to control group. However, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present significant differences compared with LFS in any of studied parameters. The findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), we found no conclusive information on which treatment is most efficient.

通督调神针刺联合低频脉冲穴位电刺激治疗脑卒中后吞咽障碍(假性球麻痹)临床研究

目的观察通督调神针刺联合低频脉冲穴位电刺激对脑卒中后吞咽障碍(DAS)(假性球麻痹)的临床疗效。方法按随机数字表法将60例DAS(假性球麻痹)患者按入院顺序随机分为治疗组与对照组各30例;两组患者均接受脑卒中西医常规治疗,并统一进行吞咽功能康复训练;对照组予普通针刺法,治疗组予通督调神针刺联合低频脉冲穴位电刺激治疗。所有患者治疗6 d为1个疗程,疗程间休息1 d,共治疗2个疗程。2个疗程后比较两组标准吞咽功能评价量表(SSA)、洼田饮水试验等级评分,并评价两组临床疗效。结果1)两组SSA量表评分在治疗后均较前降低(P<0.05),且治疗组SSA评分比对照组降低更明显(P<0.05)。2)治疗后两组患者洼田饮水试验评分比较,差异有统计学意义(P<0.05)。3)治疗组总有效率为83.33%,显著高于对照组的60.00%(P<0.05)。结论通督调神针刺联合低频脉冲穴位电刺激治疗在降低SSA评分及洼田饮水试验等级评分能力上均优于普通针刺法,可明显改善DAS(假性球麻痹)患者的吞咽功能。

中医定向透药疗法和中频脉冲电刺激治疗偏瘫肩痛的疗效差异分析

目的 分析偏瘫肩痛患者实施中医定向透药与中频脉冲电刺激治疗的效果。方法 随机选取潍坊市中医院于2022年11月—2023年11月收治的60例偏瘫肩痛患者为研究对象,掷骰子分组为透药组与刺激组,各30例。透药组行中医定向透药治疗,刺激组行中频脉冲电刺激治疗。比较两组疼痛情况、肩关节活动度、肢体功能情况及有效率。结果 治疗后,透药组疼痛评分低于刺激组,肩关节活动度高于刺激组,上肢功能及上肢自理能力优于刺激组,差异有统计学意义(P均<0.05)。透药组治疗总有效率为96.67%,高于刺激组的76.67%,差异有统计学意义(χ^(2)=5.192,P<0.05)。结论 中医定向透药疗法可大幅减轻肩痛问题,拓展肩关节活动度,提升肩关节功能,有效率高,相比中频脉冲电刺激疗效更好。

低频脉冲电刺激联合改良站立位重心转移能力训练在脑卒中后偏瘫康复治疗中的应用效果及对周围神经电生理学的影响

目的探讨低频脉冲电刺激联合改良站立位重心转移能力训练在脑卒中后偏瘫康复治疗中的应用效果及对周围神经电生理学的影响。方法选择2021年2月至2022年3月收治的60例脑卒中后偏瘫患者为研究对象,按照随机数字表法将其分为对照组与观察组,各30例。对照组采取常规康复治疗,观察组在对照组基础上施加低频脉冲电刺激联合改良站立位重心转移能力训练。比较两组的患肢肢体运动功能、步频、步速及周围神经电生理学指标。结果干预后,观察组的上肢与下肢运动功能评分均高于对照组(P<0.05)。干预后,观察组的步频、步速均高于对照组(P<0.05)。干预后,观察组的胫神经与腓总神经运动神经传导速度(MCV)与感觉传导速度(SCV)均高于对照组(P<0.05)。结论低频脉冲电刺激联合改良站立位重心转移能力训练在脑卒中后偏瘫康复治疗中的应用效果满意,可改善上肢与下肢运动功能,促进步态恢复,也能调节周围神经电生理学指标,值得推广。

低频脉冲电刺激联合任务导向康复训练在脑卒中后偏瘫康复治疗中的应用效果及对肢体肌力的影响

目的探讨低频脉冲电刺激联合任务导向康复训练在脑卒中后偏瘫康复治疗中的应用效果及对肢体肌力的影响。方法选取2020年3月至2022年2月于我院就诊的60例脑卒中后偏瘫患者为研究对象,随机将其分为对照组和观察组,各30例。两组患者入院后均给予基础治疗及对症治疗,对照组采用常规康复训练,观察组在对照组基础上加用低频脉冲电刺激联合任务导向康复训练。比较两组的干预效果。结果观察组的肢体肌力恢复总有效率高于对照组(P<0.05)。干预后,观察组的髋关节与膝关节前屈、后伸肌力高于对照组,髋关节与膝关节前屈、后伸肌张力低于对照组(P<0.05)。干预后,观察组的Fugl-Meyer下肢运动功能评分量表(FMA-LE)、Holden步行功能分级量表(FAC)评分及髋关节与膝关节活动度高于对照组(P<0.05)。结论低频脉冲电刺激联合任务导向康复训练用于脑卒中后偏瘫康复治疗中的效果显著,可增强肌力,促进肢体功能及关节活动度恢复,值得推广。

1例1型糖尿病性胃轻瘫合并乙型病毒性肝炎患者的中西医结合护理体会

本文总结1例1型糖尿病性胃轻瘫合并乙型病毒性肝炎患者的中西医结合护理经验。基于护理评估和中医辨证,在心理护理、用药护理、饮食指导等常规护理基础上,采用中频电脉冲疗法进行穴位按摩,有效改善患者恶心呕吐的症状,减轻痛苦,促进患者康复。

低频脉冲电刺激治疗运动发育迟缓患儿的疗效分析

目的:观察低频脉冲电刺激辅助治疗运动发育迟缓患儿的临床疗效。方法:回顾性选取2021年6月至2023年5月本院收治的运动发育障碍的患儿202例作为研究对象。根据治疗方式的不同将患儿分为对照组(98例,采用常规运动训练治疗)与观察组(104例,采用低频脉冲电刺激辅助常规运动训练治疗)。分析比较两组的运动能力(Peabody运动发育量表)、治疗效果、日常生活活动能力[日常生活活动能力量表(Activity of Daily Living Scale,ADL)]及并发症。结果:治疗后,两组的精细运动商(Fine Motor Quotient,FMQ)、总运动商(Total motor quotient,TMQ)及粗大运动商(Gross motor quotient,GMQ)评分均升高,且观察组的各项评分均明显高于对照组(P<0.05)。观察组治疗总有效率明显高于对照组(P<0.05)。治疗后,两组的ADL评分均显著升高,且观察组治疗后1m、3m、5mADL评分均明显高于对照组(P<0.05)。观察组的并发症发生率明显低于对照组(P<0.05)。结论:对运动发育迟缓的患儿辅以低频脉冲电刺激治疗,能提高治疗效果,改善运动能力和日常生活活动能力,减少并发症。

耳穴压豆联合中频脉冲电刺激治疗脑卒中后尿潴留的临床效果

目的 观察耳穴压豆联合中频脉冲电刺激治疗脑卒中后尿潴留(URAS)的效果。方法 选取2021年5月至2022年5月在河南省中医院治疗的62例URAS患者,根据随机数字表法分为对照组(常规治疗联合中频脉冲电刺激)和试验组(常规治疗、中频脉冲电刺激联合耳穴压豆),对比两组效果。结果 试验组治疗有效率高于对照组,并发症发生率低于对照组(χ^(2)=4.292、4.026,P=0.038、0.044);治疗后试验组膀胱功能指标(膀胱最大容量、最大尿流量、残余尿量、排尿量、排尿积分)、生活质量评分(躯体功能、社会功能、情绪功能、活力、生理功能、心理功能、精神状态)、临床症状评分(腹部坠胀、疼痛、溢尿、尿频、尿不尽、排尿不畅)优于对照组(P<0.05)。治疗前两组患者的膀胱功能评分差异无统计学意义(P>0.05);治疗后试验组评分[(15.39±2.14)分]高于对照组[(9.23±1.14)分](P<0.05)。结论 耳穴压豆和中频脉冲电刺激治疗URAS的效果显著。

5 Hz经颅脉冲电刺激对EEG影响的研究

经颅电刺激(Transcranial Electrical Stimulation,tES)可以对人体脑电图(Electroencephalogram,EEG)状态产生相应的影响,因此可以通过特定频率的电流刺激实现对人体情绪、记忆和睡眠状态的干预。本研究采用经颅脉冲电流刺激(Transcranial Pulsed Current Stimulation,tPCS)方式对人体进行经颅电刺激,主要探究在前额叶区域(F3阳极和F4阴极)的5 Hz刺激频率下0.2、0.4和0.6 mA不同刺激处方对EEG信号的影响。对采集的EEG数据进行功率谱密度、能量占比和功率比值分析,结果表明:0.6 mA电刺激的实验组提高了Theta频带(4~8 Hz)能量和Alpha前半段频带(8~10 Hz)能量,这种现象在左侧脑区的中央区(Fp1)和顶叶区(C3)更明显。同时发现5 min、5 Hz、0.4 mA的刺激处方在刺激后对Theta频带能量占比影响要高于5 min、5 Hz、0.6 mA。