Study on Early Warning Service of Low-Temperature Chilling Injury Risk in Shenyang Facilities Agriculture

On the basis of summarizing the research on disaster risk at home and abroad, Shenyang, one of the cities with the most frequent meteorological disasters, was taken as an example. Cold chilling injury is one of the major agricultural meteorological disasters, which poses a serious threat to the sustainable development of agriculture and food security in Jilin and Shenyang. Based on meteorological observation data of recent 65 years in Shenyang and microclimate monitoring data of agricultural facilities in two years, this paper makes statistical analysis by mathematical statistics and calculates the highest outdoor temperature, the lowest indoor air temperature and the highest ceiling temperature according to the meteorological grade index of agricultural facilities’ low temperature chilling damage, low temperature and other meteorological elements of grade indicators, analysis of greenhouse production trend of greenhouse climate change, and to discuss the relationship between temperature inside and outside the greenhouse and the facility microclimate conditions in agricultural cold weather disastrous and early warning services. The results showed that in the 21st century, the number of days with low temperature of ≤-20°C increased significantly compared with those in the 1970s, 1980s and 1990s. The risk of low temperature chilling injury in facility agriculture in Shenyang was dominated by mild and moderate low temperature risk. Shenyang low temperature cold damage warning services standards to reduce the impact of chilling injury on agricultural facilities and development.

Research Progress and Outlook of Low Temperature Chilling Injury in Northeast China

Focused on the major agro-meteorological disaster in northeast China area—low temperature chilling injury,the research progresses of low temperature chilling injury in northeast China were reviewed systematically.The basic concepts which included the connotation and extension of chilling damage,the circulation characteristics and effect factors which formed summer low temperature in northeast China,the prediction,forecast and defense of low temperature chilling injury were summarized and done the outlook.

Methyl Jasmonate Reduces Chilling Injury and Maintains Postharvest Quality in Peaches

Zhaohui peaches(Prunus persica Batsch)were treated with 0(CK), 1, 10 or 100 μmol L-1 methyl jasmonate(MeJA)vapor at 20℃ for 24 h before stored at 0℃ for 35 d. The untreated fruits showed chilling injury(CD symptoms after 4 wk of storage, as indicated by increased fruit firmness and reduced ex-tractable juice, which is referred as leatheriness. Treatment with 1 and 10 μmol L-1 MeJA promoted normal ripening and softening, maintained higher levels of extractable juice, titratable acidity, pectinesterase(PE)and polygalacturonase(PC)activities, inhibited increases in fruit fresh weight loss, decay incidence, electrolyte leakage and MDA content, and improved color development, thereby preventing chilling injury symptoms development and maintaining edible quality. MeJA treatment also delayed the climacteric rise in respiratory rate, but promoted ethylene production during the later period of cold storage, suggesting that ethylene may involve in CI development of peaches. These results indicate that 1 and 10 μmol L-1 MeJA treatments could be used to reduce CI development and decay incidence in peaches.

Reduction of Chilling Injury and Ultrastructural Damage in Cherry Tomato Fruits After Hot Water Treatment

The effects of hot water treatment in alleviating chilling injury and reducing ultrastructural damage of mature-green cherry tomatoes （Lycopersicun esculentum cv. cerasiform Alef） were investigated. Mature-green cherry tomato fruits were treated in water at 40℃ or 45℃ for 5 min or 15 min, and then stored at 5℃ for 19 days followed by ripening at 20℃. Water treatment at 40℃ for 15 min increased tolerance of cherry tomato fruits to chilling stress, indicating as low outbreak of skin lesion, high color a＊ value, and low electrolyte leakage. Treated fruits showed typical climacteric respiration and developed normal red color with chlorophyll degradation and lycopene accumulation during ripening, while fruits without treatment failed to develop red color and suffered skin lesion. After 19 days of chilling, heated fruits showed the conversion of chloroplast to chromoplast with the disappearance of thylakoids. Mitochondria and other cell organelles were not adversely affected in treated fruits. However, ultrastructures in pericarp cells in control fruits severely damaged with extensive disorganization of cytoplasm, swelled chloroplasts, distorted and unstacked thylakoids. Chloroplast was the first and most severely impacted organelle by chilling stress. Hot water treatment （40℃ for 15 min） before storage alleviated chilling injury in cherry tomato fruits. The results suggest that chilling injury is related with the damage of cell structure under chilling stress.

Salicylic acid alleviates postharvest chilling injury of sponge gourd(Luffa cylindrica)

Effect of salicylic acid（SA）on chilling injury（CI）of sponge gourd during storage（9 days,9°C）plus shelf life（2 days,20°C）was evaluated in this study.SA treatment at the concentration of 1.5 mmol L^-1 significantly reduced postharvest CI of sponge gourds.Besides,the application of SA could effectively decrease the electrolyte leakage,reduce the accumulation of malondialdehyde（MDA）and total phenolics,enhance the activities of antioxidant enzymes such as superoxide dismutase（SOD）,catalase（CAT）and ascorbate peroxidase（APX）,and inhibit the activities of phenylalanine ammonia-lyase（PAL）and polyphenol oxidase（PPO）.The beneficial effects of SA could be attributed to preserved membrane integrity,inhibited membrane peroxidation,enhanced antioxidant system and suppressed activities of browning related enzymes.In a sense,SA as a postharvest tool may be commercially used in alleviating CI of sponge gourd.

Chilling Injury, Physicochemical Properties, and Antioxidant Enzyme Activities of Red Pitahaya (Hylocereus polyrhizus) Fruits under Cold Storage Stress

Low-temperature storage is extensively used to optimize the postharvest life of various fresh fruits.However,red pitahaya(Hylocereus polyrhizus)fruits are sensitive to chilling injury(CI),which leads to the limitation of low-temperature storage.In this study,red pitahaya fruits were stored at 2,4,6,8,and 10℃,respectively,for 27 days to determine the appropriate storage temperature.During the storage of red pitahaya fruits,storage at 8℃ was more effective in suppressing decay and maintaining quality than other low temperatures.Lowtemperature(2,4,and 6℃)storage decreased weight loss(WL)and maintained higher content of titratable acidity(TA),soluble sugars(SS),and total phenolics(TP)but different degrees of CI were detected.No CI was observed at 8℃ and 10℃.Red pitahay as stored at 8 and 10℃ were associated with better color evaluation,lower electrolyte leakage(EL),respiration rate,and lipoxygenase(LOX)activity,and higher fruit firmness,superoxide dismutase(SOD)activity,and catalase(CAT)activity.However,higher storage temperature(10℃)resulted in higher metabolic activity leading to lower quality and antioxidant capacities compared with 8℃.Therefore,our results demonstrated that red pitahaya stored at 8℃ exhibited a protective effect on fruit quality and resisted CI development during storage.

Regulations of m^(6)A methylation on tomato fruit chilling injury

Tomato fruit are sensitive to chilling injury(CI)during cold storage.Several factors have been discovered to be involved in chilling injury of tomato fruit.Plant hormones play an important regulatory role,however,the relationship between chilling injury and N6-methyladenosine(m^(6)A)methylation of transcripts in plant hormone pathways has not been reported yet.In order to clarify the complex regulatory mechanism of m^(6)A methylation on chilling injury in tomato fruit,Nanopore direct RNA sequencing was employed.A large number of enzymes and transcription factors were found to be involved in the regulation process of fruit chilling injury,which were associated with plant hormone,such as 1-aminocyclopropane 1-carboxylate synthase(ACS),aspartate aminotransferase(AST),auxin response factor(ARF2),ethylene response factor 2(ERF2),gibberellin 20-oxidase-3(GA20ox)and jasmonic acid(JA).By conjoint analysis of the differential expression transcripts related to chilling injury andm^(6)Amethylation differential expression transcripts 41 differential expression transcripts were identified involved in chilling injury including 1-aminocyclopropane-1-carboxylate oxidase(ACO)and pectinesterase(PE)were down-regulated and heat shock cognate 70 kD protein 2(cpHSC70),HSP70-binding protein(HspBP)and salicylic acid-binding protein 2(SABP2)were up-regulated.Our results will provide a deeper understanding for chilling injury regulatory mechanism and post-harvest cold storage of tomato fruit.

Effect of Exogenous Salicylic Acid on Physiological Parameters of Cucumber and Tomato Fruits During Chilling Injury Temperature Storage

Harvested tomato(Lycopersicum esculentum Mill)and cucumber (Cucumis Sativus L.)were immersed in 0, 0.01 g/L, 0.05 g/L, 0.1 g/L or 0 g/L, 0.001 g/L, 0.01 g/L, 0.05 g/L, 0.1 g/L salicylic acid solutions for 15 min, respectively. Some of physiological parameters of the fruits related to chilling injury were measured during cold storage (2℃±1℃ ). It showed that the cell membrane electrolyte leakage, MDA content and free proline content in tomato with 0.01 g/L and 0.1 g/L SA were lower than those of control to a various extent. The immersion in 0.001 g/L SA could significantly decrease the cell membrane electrolyte leakage and MDA content of cucumber stored at chilling injury temperature as well as decrease free proline content to some extent.

Seed Priming Improves Chilling Stress Tolerance in Rice (Oryza sativa L.) Seedlings

Chilling is one of the major abiotic stresses for plants,especially for rice cultivation.Many essential metabolic processes for growth and development are temperature-dependent.In that case,reducing the negative effects of cold stress using exogenous chemicals is a possible option.Therefore,the current study examined the effects of pre-sowing seed treatment with different chemicals,viz.hydrogen peroxide(H_(2)O_(2)),salicylic acid(SA),calcium chloride(CaCl_(2)),thiourea(TU),and citric acid(CA)on the germination of rice seeds(cv.BRRI dhan28)under chilling environments.Rice seeds were soaked in distilled water(control),10 mM CA,2 mM SA,10 mM CaCl_(2),10 mM H_(2)O_(2),and 10 mM TU solutions for 24 h.After that,seeds were exposed to chilling stress by incubating at 4±1℃ for 8 h,followed by at 25±2℃ for 16 h for 7 days.Exposure to chilling stress significantly reduced thefinal germination percent(13.6%),germination rate index(36.0%),coefficient of the velocity(25.0%),shoot fresh weight(44.4%),and root fresh weight(60.5%).Moreover,chilling induced oxidative damage and reduced the activity of antioxidant enzymes(catalase and ascorbate peroxidase).In contrast,treatments with H_(2)O_(2),SA,CaCl_(2),TU,and CA considerably enhanced germination indices and seedling growth compared to chilling stress condi-tions.The study showed that priming with H_(2)O_(2),SA,CaCl_(2),TU,and CA significantly boosted antioxidant enzyme activities and reduced MDA and H_(2)O_(2) contents in chilling-stressed rice plants,indicating less oxidative stress and improved tolerance.Principal component analysis showed that among these priming agents,H_(2)O_(2),SA,and CA are most effective in chilling stress mitigation.Therefore,using seed-treating chemicals to combat the effect of chilling stress can help rice seedlings grow better in the winter season.

Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress

Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage.Therefore,advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value.This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI,proline metabolism,and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control),0.1 (MT1),and 0.2 mmol L^(-1)(MT2) melatonin solution for 30 min.The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control.These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis,such as pyrroline-5-carboxylate synthetase (P5CS),pyrroline-5-carboxylate reductase (P5CR),ornithine D-aminotransferase (OAT),P5CS2,P5CR2,and OAT3.Additionally,proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group.Thus,melatonin treatment has regulated proline metabolism resulting in the accumulation of proline,subsequently contributing to enhancing the chilling tolerance of‘Keitt’mango fruit.

The location of 5′-nucleotidase in seedling cells in Yunnan rice varieties and the change of enzyme activity after chilling injury

The seedlings of three rice varieties (Damagu,Ligeng 2, and Xiuzinuo) were cultivated at 25 ±1℃. After chilling injury (at 2~C), blacksediments of lead phosphate appeared on plas-

Melatonin treatment induces chilling tolerance by regulating the contents of polyamine,γ-aminobutyric acid,and proline in cucumber fruit

The mechanism of melatonin(MT)induced chilling tolerance in harvested cucumber fruit was investigated at commercial maturity.In this study,cucumber fruits were treated with 100μmol L^(-1) MT at 4℃ and 90% relative humidity for 15 d of storage.In comparison with the control,cucumber treatment with MT resulted in reduced chilling injury(CI),decreased electrolyte leakage and enhanced firmness.The fruits treated with MT showed higher chlorophyll contents in storage conditions with suppressed chlorophyllase enzyme activity.MT treatment increased arginine decarboxylase(ADC)and ornithine decarboxylase(ODC)enzyme activities.Moreover,enhanced expression of the Cucumis sativus ADC(CsADC)and C.sativus ODC(CsODC)genes resulted in the accumulation of polyamine contents.Similarly,proline levels exhibited higher levels among treated fruits.Meanwhile,the proline synthesizing enzymes △1-pyrroline-5-carboxylate syntheses(P5CS)and ornithine aminotransferase(OAT)were significantly increased,while a catabolic enzyme of proline dehydrogenase(PDH)activity was inhibited by treatment.In addition,MT induced expression of C.sativus OAT(CsOAT)and C.sativus P5CS(CsP5CS)genes.Cucumber fruits treated with MT also exhibited higher γ-aminobutyric acid(GABA)content by enhanced GABA transaminase(GABA-T)and glutamate decarboxylase(GAD)enzyme activities and a higher C.sativus GAD(CsGAD)gene expression.To sum up,the results show that MT treatment enhanced chilling tolerance,which was associated with the regulation of polyamines,as well as proline and γ-aminobutyric acid.

Effects of 1-MCP on proline,polyamine,and nitric oxide metabolism in postharvest peach fruit under chilling stress

Peach(Prunus persica Batsch‘Yuhualu’)fruit are sensitive to chilling injury(CI).Proline,polyamine(PA),and nitric oxide(NO)are important small regulators of various metabolic pathways under chilling stress that mitigate CI.Ethylene is known to promote senescence and CI,while 1-methylcyclopropene(1-MCP)is an antagonist that inhibits the effects of ethylene.However,how1-MCP and ethylene affect proline,PA,and NO levels under chilling stress remains unclear.To address these questions,1-MCP(1μL·L^(−1))and ethylene(1μL·L^(−1))treatments were applied to peach fruit.Fruit were stored at 4°C for 28 d,then moved to 25°C for 3 d immediately after cold storage.Peach fruit exhibited CI symptoms after 7 d of cold storage with enhanced electrolyte leakage and malondialdehyde contents.The 1-MCP treatment significantly(P<0.05)restrained peach CI,and fruit did not exhibit CI symptoms until 14 d of cold storage.Proline and PAs in peach under chilling stress weremostly synthesized from glutamate and arginine,which were catalyzed by1-pyrroline-5-carboxylate synthetase and arginine decarboxylase,respectively.1-MCPtreated fruit exhibited higher proline and PA contents and enhanced chilling tolerance compared to the control,while ethylene-treated fruit had lower proline and PA contents and reduced chilling tolerance.Ethylene-treated fruit,which exhibited more severe CI symptoms compared to the control,had significantly(P<0.05)lower NO contents and NO synthase activities.However,NOmay not be a direct acting factor in 1-MCPinduced chilling tolerance,as 1-MCP-treated fruit had lower NO contents and NO synthase activities compared to the control.In conclusion,proline and PA clearly played direct and important roles in 1-MCP-induced peach chilling tolerance,while NO may not be actively involved.

Effect of Maturity Stage on the Gene Expression of Antioxidative Enzymes in Cucumber (Cucumis sativus L.) Fruits Under Chilling Stress

The gene expression pattems of antioxidative enzymes in cucumber （Cucumis sativus L.） fruits at four different maturity stages, immature （3-8 d after anthesis （DAA）, mature （9-16 DAA）, breaker （17-22 DAA）, and yellow （35-40 DAA）, were determined before and after cold storage at 2℃ for 9 d and after subsequent rewarming at 20℃ for 2 d. The electrolyte leakage and malondialdehyde content in cucumber fruits were increased after cold storage and subsequent rewarming. Increased expressions of peroxidase, ascorbate peroxidase （APX）, and monodehydroascorbate reductase after cold storage played an important role in cucumber fruits to cope with chilling injury. The elevated cyt-superoxide dismutase, catalase, APX and dehydroascorbate reductase after subsequent rewarming in cucumber fruits facilitated the recovery from chilling stress. The highest expression levels of all the seven antioxidative enzyme genes in yellow fruits might be responsible for the enhanced chilling tolerance. Cucumber fruits at earlier developmental stages was more susceptible to chilling stress than those at later stages. The relative higher gene expressions of antioxidative enzymes genes at earlier developmental stages may be the responses to the sever oxidative stress caused by chilling injury.

Transcriptome and genome analysis to identify C2H2 genes participating in low-temperature conditioning-alleviated postharvest chilling injury of peach fruit

Objectives:This work intended to identify candidate C2H2 genes participating in low-temperature conditioning(LTC)-alleviated postharvest chilling injury of peach fruit.Materials and Methods:For LTC treatment,fruit were pre-stored at 10℃for 5 d and then transferred to 0℃storage.Fruit firmness was measured by a hardness tester.H_(2)O_(2)content was determined by luminosity measurement model using a multifunctional enzyme labeler.Identification of C2H2 family members was performed by HMMSCAN according to peach genome.The cis-acting element of gene promoters was analyzed using the Plant CARE website.Weighted gene coexpression network analysis(WGCNA)was performed by the WGCNA package in the BMK Cloud platform.Results:LTC treatment decreased flesh browning rate and H_(2)O_(2)production of‘Beijing No.9’peach.Transcription factor identification of differentially expressed genes in 0℃and the LTC treatment indicated that peach C2H2 participated in the regulation of chilling injury.A total of 47 C2H2 genes were identified based on peach genome.Real-time quantitative polymerase chain reaction(qRT-PCR),phylogenetic analysis and promoter cis-acting element analysis revealed that ZFP21 was involved in the regulation of LTC-alleviated chilling injury in peach.WGCNA and dual luciferase assay suggested that ZFP21 participated in LTC-alleviated chilling injury by downregulating the expression of reactive oxygen species-related genes Rboh.Conclusions:Our investigation,based on genome and RNA-seq,revealed that ZFP21 was involved in LTC treatment-alleviated chilling injury of peach fruit.This work is useful for the identification of peach cold tolerance-related genes and the study of C2H2 family in peach.

海南岛冬季瓜菜寒害气象指标及发生规律研究

利用海南岛18个气象站的1971—2020年冬季(12月至翌年2月)气象数据和灾情资料,构建考虑阴雨寡照影响、能量化寒害过程影响程度且具备生物学特征的冬季瓜菜寒害气象指标。在此基础上结合Mann-Kendall趋势检验法和ArcGIS技术,得出海南岛冬季瓜菜寒害时空变化规律。结果表明:冬季各月及各月轻至重度瓜菜寒害发生概率较高的地区主要分布在海南岛西北部和中部,出现概率较低的地区主要分布在西部、南部、东部沿海一带。冬季各月瓜菜寒害均主要以轻、中度等级为主,二者占比高达88.3%。3个月中,1月瓜菜寒害发生次数最多(占比达48.6%),瓜菜轻至重度寒害亦均在1月出现最多(占比分别为50.2%、43.4%和40.2%)。近50年海南岛冬季各月瓜菜寒害年发生次数不显著减少的站点占多数(占比分别为83.3%、94.4%和72.2%),各月不同等级瓜菜寒害中除2月重度瓜菜寒害年发生次数呈增加趋势的站点较多(占比38.9%)外,其余等级瓜菜寒害年发生次数不显著减少的站点亦占多数(占比61.1%~88.9%)。冬季各月不同等级瓜菜寒害的影响范围均按寒害轻至重顺序明显缩小。3个月之间相比,12月和2月瓜菜寒害影响范围接近且明显小于1月,轻度和中度寒害影响范围月之间亦表现出类似规律,重度寒害影响范围12月和1月接近且明显大于2月。近50年海南岛冬季各月及各月所有等级瓜菜寒害影响范围均在缩小。总体而言,近50年海南岛冬季瓜菜寒害年发生次数减少、影响范围缩小,对海南岛冬季瓜菜种植有利。但2月东、中、西部地区瓜菜重度寒害年发生次数在增加,需加以关注。

近60年新疆棉花苗期低温冷害时空变化规律

利用新疆不同棉区46个国家基本气象站1961-2022年日平均气温,结合棉花苗期低温冷害监测指标,通过气候倾向率、MK突变检验、累积距平等方法,分析棉花苗期轻度、中度、重度低温冷害的时间和空间变化规律,为新疆不同棉区制定合理的防灾减灾措施提供科学依据。结果表明:1961-2022年全疆棉区苗期气温呈极显著上升趋势,气候倾向率为0.30℃·10a^(-1)。北疆、南疆和东疆棉区分别在1999年、2004年和2003年轻度苗期冷害发生频次较少。北疆棉区于2006年、南疆和东疆棉区于2008年中度苗期冷害发生频次较少。1975年、2003年北疆和南疆棉区分别进入重度苗期冷害较少发生的阶段。近60a新疆棉花苗期低温冷害多发生于20世纪80和90年代,轻度和中度冷害发生年份分别占研究时段的79.0%和37.1%,重度冷害发生年份仅占8.1%。棉花苗期低温冷害多发生于北疆棉区,其次是南疆棉区,东疆低温冷害发生最少。

基于高分辨格点数据东北水稻延迟型冷害风险评估及保险费率厘定

利用5km×5km空间分辨率3h时间分辨率的气象格点数据集,研究东北地区水稻延迟型冷害风险及其保险费率的厘定。基于东北地区1981-2010年5-9月平均温度的格点数据集和99个气象站的站点观测数据,以相关系数和均方根误差评价格点气象数据在东北地区的可用性。以日平均气温稳定通过10℃和18℃的日数作为获取水稻气候安全种植区域的指标,在水稻气候安全种植范围内,分析东北地区水稻延迟型冷害的空间分布特征,确定保险费率。结果表明,东北地区1981-2010年5-9月平均温度气象站点观测数据与格点数据的相关系数高,均方根误差小,表明格点数据在东北地区可用。水稻气候安全种植区域占东北的56.5%,主要分布在辽宁省、吉林省中西部、黑龙江省西南部和东北部、蒙东西部及东部与辽宁和吉林省接壤的区域。在水稻气候安全种植区内,水稻延迟型冷害发生频率呈南低北高,中间低东西高的分布特征,且重度延迟型冷害发生频率最高。低温冷害风险指数空间分布与之相似,内蒙古西部和东北部、黑龙江北部和吉林西部局部地区是风险指数的高值区。东北地区1981-2010年水稻延迟型冷害的天气指数保险费率在空间分布上与东北地区低温冷害风险指数的空间分布相似,呈南部低,北部高,中间低,东西高的特征,整个区域的保险费率在0.010~0.094,可为保险公司制定具体费率提供参考。

黑龙江省大豆延迟型冷害指标构建及检验

黑龙江省大豆生长季冷害发生频次高、影响重,构建黑龙江省大豆延迟型冷害指标,对大豆防灾减灾、保障大豆安全生产具有重要意义。利用1961-2020年黑龙江省73县(市、区)大豆产量资料及气象站逐日温度资料,以5-9月各月月平均温度之和的距平值为致灾因子,利用致灾因子和减产率的线性关系确定冷害阈值,构建黑龙江省大豆延迟型冷害指标并验证。结果表明:研究区大豆在5个种植区轻度冷害阈值分别为-1.8℃、-1.9℃、-1.9℃、-2.0℃、-2.0℃,中度冷害阈值分别为-2.0℃、-2.2℃、-2.3℃、-2.4℃、-2.6℃,重度冷害阈值分别为-2.2℃、-2.5℃、-2.7℃、-2.9℃、-3.1℃。经检验,大豆延迟型冷害指标对冷害发生趋势判识平均准确率83%,对冷害发生等级判识平均准确率62%,典型冷害年判识冷害平均准确率93%。综上所述,大豆延迟型冷害指标界定及评价黑龙江省大豆生长季延迟型冷害准确率较高,指标获取容易且实用性强。

基于理化分析和机器学习研究低温气调对采后番荔枝冷害的影响

本文旨在探究气调包装对番荔枝冷害的影响,并通过机器学习解析各指标与冷害的关系。以室温(RT,25℃)、低温(LT,10℃)和低温气调包装(CA,10℃)贮藏条件下的果实为试验材料,测定7 d内果肉硬度、冷害指数(CI)、总酚、相对电导率(EC)、苯丙氨酸裂解酶(PAL)活性等理化指标。结果表明,低温贮藏有效延缓番荔枝后熟,抑制软化,但易造成冷害现象,表现为果肉出现水浸状损伤,细胞膜透性增加,丙二醛积累。在此基础上,建立多种机器学习算法,筛选出预测CI的最优模型为Ridge回归。解释性分析(SHapley Additive exPlanations,SHAP)显示,贮藏时间、可溶性固形物、失重率、可溶性蛋白和总酚对模型贡献较大,与低温胁迫紧密联系。低温结合气调通过增加可溶性物质和酚类含量,提高机体渗透调节和清除活性氧能力,从而维持膜结构完整性以缓解低温冷害。