Determining the suitable areas for winter wheat under climate change and assessing the risk of freezing injury are crucial for the cultivation of winter wheat.We used an optimized Maximum Entropy(MaxEnt)Model to predi...Determining the suitable areas for winter wheat under climate change and assessing the risk of freezing injury are crucial for the cultivation of winter wheat.We used an optimized Maximum Entropy(MaxEnt)Model to predict the potential distribution of winter wheat in the current period(1970-2020)and the future period(2021-2100)under four shared socioeconomic pathway scenarios(SSPs).We applied statistical downscaling methods to downscale future climate data,established a scientific and practical freezing injury index(FII)by considering the growth period of winter wheat,and analyzed the characteristics of abrupt changes in winter wheat freezing injury by using the Mann-Kendall(M-K)test.The results showed that the prediction accuracy AUC value of the MaxEnt Model reached 0.976.The minimum temperature in the coldest month,precipitation in the wettest season and annual precipitation were the main factors affecting the spatial distribution of winter wheat.The total suitable area of winter wheat was approximately 4.40×10^(7)ha in the current period.In the 2070s,the moderately suitable areas had the greatest increase by 9.02×10^(5)ha under SSP245 and the least increase by 6.53×10^(5)ha under SSP370.The centroid coordinates of the total suitable areas tended to move northward.The potential risks of freezing injury in the high-latitude and-altitude areas of the Loess Plateau,China increased significantly.The northern areas of Xinzhou in Shanxi Province,China suffered the most serious freezing injury,and the southern areas of the Loess Plateau suffered the least.Environmental factors such as temperature,precipitation and geographical location had important impacts on the suitable area distribution and freezing injury risk of winter wheat.In the future,greater attention should be paid to the northward boundaries of both the winter wheat planting areas and the areas of freezing injury risk to provide the early warning of freezing injury and implement corresponding management strategies.展开更多
[Objective] The aim was to explore effects of occurrence frequency of freezing injury on rapeseed production and remedial measures. [Method] The re- search utilized three turns of freezing injury during the growth per...[Objective] The aim was to explore effects of occurrence frequency of freezing injury on rapeseed production and remedial measures. [Method] The re- search utilized three turns of freezing injury during the growth period of winter-sown rapeseed in 2013, analyzed the freezing injury resistibility and the remedial mea- sures of ten varieties(combination). [Result] The results showed that for different va- rieties (combinations) of rapeseed, compared cutting ones with non-cutting ones, the plant silique, seeds number and seed weight increased in diverse level, "however, the plant yield remained the same. Correlation analysis suggested that freezing in- jury had the greatest impact on plant silique. [Conclusion] After cutting processing, yield traits were able to partially recover and showed kind of positive correlation with Plant yield.展开更多
[Objective]The paper was to explore the influence of near-surface low temperature on cultivation of soft-seed pomegranate,and to provide guidance for planting location of soft-seed pomegranate.[Method]Taking 10 soft-s...[Objective]The paper was to explore the influence of near-surface low temperature on cultivation of soft-seed pomegranate,and to provide guidance for planting location of soft-seed pomegranate.[Method]Taking 10 soft-seed pomegranate planting plots under different site conditions as the research objects,the near-surface low temperature of 45-50 cm was dynamically monitored from December 1,2018 to February 20,2019,and comparative analysis was made based on the local meteorological data over the same period.[Result]The near-surface low temperature of each temperature monitoring point was lower than the local meteorological data,which were all in the range of low temperature causing freezing in-jury of pomegranate trees,but the degree of freezing injury was different.The variation of near-surface low temperature was positively correlated with the altitude of terrain,but negatively correlated with the difference of topography and landform.When the local topography and landform were similar,the accumulation time of near-surface low temperature was negatively correlated with the altitude of terrain,while the duration of low tem-perature directly affected the degree of freezing injury.[Conclusion]The development of soft-seed pomegranate cultivation in Tunisia along Huang Mangling region in Henan Province refers to the local meteorological data.Meantime,it is also necessary to pay attention to the impact of regional microclimate environment,especially early monitoring of near-surface temperature to select suitable site and natural conditions.展开更多
On the basis of summarizing the research on disaster risk at home and abroad, Shenyang, one of the cities with the most frequent meteorological disasters, was taken as an example. Cold chilling injury is one of the ma...On the basis of summarizing the research on disaster risk at home and abroad, Shenyang, one of the cities with the most frequent meteorological disasters, was taken as an example. Cold chilling injury is one of the major agricultural meteorological disasters, which poses a serious threat to the sustainable development of agriculture and food security in Jilin and Shenyang. Based on meteorological observation data of recent 65 years in Shenyang and microclimate monitoring data of agricultural facilities in two years, this paper makes statistical analysis by mathematical statistics and calculates the highest outdoor temperature, the lowest indoor air temperature and the highest ceiling temperature according to the meteorological grade index of agricultural facilities’ low temperature chilling damage, low temperature and other meteorological elements of grade indicators, analysis of greenhouse production trend of greenhouse climate change, and to discuss the relationship between temperature inside and outside the greenhouse and the facility microclimate conditions in agricultural cold weather disastrous and early warning services. The results showed that in the 21st century, the number of days with low temperature of ≤-20°C increased significantly compared with those in the 1970s, 1980s and 1990s. The risk of low temperature chilling injury in facility agriculture in Shenyang was dominated by mild and moderate low temperature risk. Shenyang low temperature cold damage warning services standards to reduce the impact of chilling injury on agricultural facilities and development.展开更多
Non-freezing cold injury is a prevalent cause of peripheral nerve damage, but its pathogenic mechanism is poorly understood, and treatment remains inadequate. Glucocorticoids have anti-inflammatory and lipid peroxidat...Non-freezing cold injury is a prevalent cause of peripheral nerve damage, but its pathogenic mechanism is poorly understood, and treatment remains inadequate. Glucocorticoids have anti-inflammatory and lipid peroxidation-inhibiting properties. We therefore examined whether dexamethasone, a synthetic glucocorticoid compound, would alleviate early-stage non-freezing cold injury of the sciatic nerve. We established Wistar rat models of non-freezing cold injury by exposing the left sciatic nerve to cold(3–5°C) for 2 hours, then administered dexamethasone(3 mg/kg intraperitoneally) to half of the models. One day after injury, the concentration of Evans blue tracer in the injured sciatic nerve of rats that received dexamethasone was notably lower than that in the injured sciatic nerve of rats that did not receive dexamethasone; neither Evans blue dye nor capillary stenosis was observed in the endoneurium, but myelinated nerve fibers were markedly degenerated in the injured sciatic nerve of animals that received dexamethasone. After dexamethasone administration, however, endoneurial vasculopathy was markedly improved, although damage to the myelinated nerve fiber was not alleviated. These findings suggest that dexamethasone protects the blood-nerve barrier, but its benefit in non-freezing cold injury is limited to the vascular system.展开更多
Using Tongxian No.2 as material, the effects of different film-covering time, different sowing time and different planting density on the occurrence of freeze injury and yield of fresh broad beans were investigated. T...Using Tongxian No.2 as material, the effects of different film-covering time, different sowing time and different planting density on the occurrence of freeze injury and yield of fresh broad beans were investigated. The randomized block design was adopted. The results showed that with the delayed film covering, the incidence of mild freeze injury and number of headless seedlings were increased correspondingly, but the yield was increased; with the delayed sowing, the branch number per plant, effective branch number per plant, incidence of mild freeze injury and number of headless seedlings were all reduced, and the broad beans, sowed on September 30 th, obtained the highest yield; planting density showed on effect on the occurrence of freeze injury, and the yield was increased with the increase of planting density. Under the same film-covering time, the incidence of freeze injury was reduced with the delayed sowing time and it showed no changes when planting density was changed, but the yield was increased with the increase of planting density and it was highest when broad bean seeds were sowed on September 30th;under the same sowing time, the incidence of freeze injury was increased with the delayed film-covering time and it showed no changes when planting density was changed, and the yield was increased with the delayed film-covering time and increased planting density; under the same planting density, the incidence of freeze injury was increased with the delayed film-covering time but was reduced with the delayed sowing time, and the yield was increased with the delayed film-covering time and it was highest when the broad bean seeds were sowed on September30 th. Under same film-covering time and sowing time, the total branch number per plant and effective branch number per plant were reduced, but the yield was increased with the increase of planting density; under same film-covering time and planting density, the incidence of freeze injury was reduced with the delayed sowing time, and the yield was highest when broad bean seeds were sowed on September30th; under same sowing time and planting density, the incidence of freeze injury and the yield were all increased with the delayed film-covering time.展开更多
Freeze injury is an usual disaster for winter wheat in Shanxi Province, China, and monitoring freeze injury is of important economic significance. The aim of this article is to monitor and analyze the winter wheat fre...Freeze injury is an usual disaster for winter wheat in Shanxi Province, China, and monitoring freeze injury is of important economic significance. The aim of this article is to monitor and analyze the winter wheat freeze injury using remote sensing data, to monitor the occurrence and spatial distribution of winter wheat freeze in time, as well as the severity of the damage. The winter wheat freeze injury was monitored using multi-temporal moderate-resolution imaging spectroradiometer (MODIS) data, combined with ground meteorological data and field survey data, the change of normalized difference vegetation index (NDVI) before and after freeze injury was analyzed, as well as the effect of winter wheat growth recovery rate on yield. The results showed that the NDVI of winter wheat decreased dramatically after the suffering from freeze injury, which was the prominent feature for the winter wheat freeze injury monitoring. The degrees of winter wheat freeze injury were different in the three regions, of which, Yuncheng was the worst severity and the largest freeze injury area, the severity of freeze injury correlates with the breeding stage of the winter wheat. The yield of winter wheat showed positive correlation with its growth recovery rate (r=0.659^** which can be utilized to monitor the severity of winter wheat freeze injury as well as its impact on yield. It can effectively monitor the occurrence and severity of winter wheat freeze injury using horizontal and vertical profile distribution and growth wheat freeze injury in Shanxi Province. recovery rate, and provide a basis for monitoring the winter展开更多
Winter wheat freeze injury is one of the main agro-meteorological disasters affecting wheat production. In order to evaluate the severity of freeze injury on winter wheat systematically, we proposed a grey-system mod...Winter wheat freeze injury is one of the main agro-meteorological disasters affecting wheat production. In order to evaluate the severity of freeze injury on winter wheat systematically, we proposed a grey-system model (GSM) to monitor the degree and the distribution of the winter wheat freeze injury. The model combines remote sensing (RS) and geographic information system (GIS) technology. It gave examples of wheat freeze injury monitoring applications in Gaocheng and Jinzhou of Hebei Province, China. We carried out a quantitative evaluation method study on the severity of winter wheat freeze injury. First, a grey relational analysis (GRA) was conducted. At the same time, the weights of the stressful factors were determined. Then a wheat freezing injury stress multiple factor spatial matrix was constructed using spatial interpolation technology. Finally, a winter wheat freeze damage evaluation model was established through grey clustering algorithm (GCA), and classifying the study area into three sub-areas, affected by severe, medium or light disasters. The evaluation model were verified by the Kappa model, the overall accuracy reached 78.82% and the Kappa coefficient was 0.6754. Therefore, through integration of GSM with RS images as well as GIS analysis, quantitative evaluation and study of winter wheat freeze disasters can be conducted objectively and accurately, making the evaluation model more scientific.展开更多
In forest ecosystems, gap formation changes the allocation of abiotic resources and thus affects the survival and growth of understory plants. However, how tree seedling survival and growth respond to low-temperature ...In forest ecosystems, gap formation changes the allocation of abiotic resources and thus affects the survival and growth of understory plants. However, how tree seedling survival and growth respond to low-temperature events and the influencing mechanisms remain unclear. To clarify how low-temperature event limits the survival and growth of tree seedlings in the montane regions of eastern Liaoning Province, northeast China, we investigated temperature and light intensity within secondary forest gaps, and the survival and growth of Juglans mandshurica seedlings after a low-temperature event in the spring of 2014. Damage to seedlings due to low temperature sig- nificantly varied in different aspects. Seedlings in gaps on southeast-facing slopes were the most seriously damaged, followed by those in gaps on northeast-facing slopes. In contrast, seedlings in west-facing gaps and in control plots without slope aspect were not damaged. The freezing injury index for seedlings was negatively correlated with minimum temperature (r = - 0.608, P 〈 0.01), but it was positively correlated with light intensity (r= 0.818, P 〈 0.01). In addition, height and root collar diameter of damaged seedlings were significantly lower than those of the undamaged seedlings (P 〈 0.01) during the early growing season (April-July), but no significant difference were observed during the late growing season (July-Oc- tober) (P 〉 0.05). The extent of seedling damage was directly related to slope aspect. Low temperature and high light intensity were found to be the dominant factors affecting extent of damage to seedlings on southeast- and northeast-facing slopes.展开更多
The susceptibility was compared between murine bone marr ow hemopoietic cells and splenic lymphocytes to four major factors of cryopreservation process: toxicity of dimethyl sulfoxide (DMSO), cooling rate,Tris-NH4Cl t...The susceptibility was compared between murine bone marr ow hemopoietic cells and splenic lymphocytes to four major factors of cryopreservation process: toxicity of dimethyl sulfoxide (DMSO), cooling rate,Tris-NH4Cl treatment, and dilution after thawing. When the concentration of DMSO was over 20%, the proliferative function or viability of both kinds of cells decreased markedly. Injury to hemopoietic stem cells and lymphoeytes was found more severe in rapid cooling than in slow cooling, and the intensity of iujury seemed alike under the same fast cooling condition. Pretreatment of bone marrow cells with Tris-NH4Cl did not significantly accentuate the injury of frozen-thawed hemopoietic stem cells. Dilution of the frozen-thawed cells in ice bath or at a fast rate was harmful to both cells. No significant differential susceptibilities of the two kinds of cells to each factor were found. These results indicate that selective destruction or inactivation of lymphocytes by cold treatment among bone marrow cells may be impossible.展开更多
Sperm freezing is widely used in ART clinics around the globe. Very little has actually changed with respect to cryopreservation protocols and methodology of freezing over the last 50 years. The aim of this paper is t...Sperm freezing is widely used in ART clinics around the globe. Very little has actually changed with respect to cryopreservation protocols and methodology of freezing over the last 50 years. The aim of this paper is to briefly review the basic principles that underlie freezing and ice crystal formation and also provide a brief overview of newer sperm freezing techniques like sperm vitrification and freeze drying of sperm.展开更多
基金supported by the National Natural Science Foundation of China(31201168)the Basic Research Program of Shanxi Province,China(20210302123411)the earmarked fund for Modern Agro-industry Technology Research System,China(2022-07).
文摘Determining the suitable areas for winter wheat under climate change and assessing the risk of freezing injury are crucial for the cultivation of winter wheat.We used an optimized Maximum Entropy(MaxEnt)Model to predict the potential distribution of winter wheat in the current period(1970-2020)and the future period(2021-2100)under four shared socioeconomic pathway scenarios(SSPs).We applied statistical downscaling methods to downscale future climate data,established a scientific and practical freezing injury index(FII)by considering the growth period of winter wheat,and analyzed the characteristics of abrupt changes in winter wheat freezing injury by using the Mann-Kendall(M-K)test.The results showed that the prediction accuracy AUC value of the MaxEnt Model reached 0.976.The minimum temperature in the coldest month,precipitation in the wettest season and annual precipitation were the main factors affecting the spatial distribution of winter wheat.The total suitable area of winter wheat was approximately 4.40×10^(7)ha in the current period.In the 2070s,the moderately suitable areas had the greatest increase by 9.02×10^(5)ha under SSP245 and the least increase by 6.53×10^(5)ha under SSP370.The centroid coordinates of the total suitable areas tended to move northward.The potential risks of freezing injury in the high-latitude and-altitude areas of the Loess Plateau,China increased significantly.The northern areas of Xinzhou in Shanxi Province,China suffered the most serious freezing injury,and the southern areas of the Loess Plateau suffered the least.Environmental factors such as temperature,precipitation and geographical location had important impacts on the suitable area distribution and freezing injury risk of winter wheat.In the future,greater attention should be paid to the northward boundaries of both the winter wheat planting areas and the areas of freezing injury risk to provide the early warning of freezing injury and implement corresponding management strategies.
基金Supported by Rapeseed Industry System Construction of Yunnan Agricultural Department~~
文摘[Objective] The aim was to explore effects of occurrence frequency of freezing injury on rapeseed production and remedial measures. [Method] The re- search utilized three turns of freezing injury during the growth period of winter-sown rapeseed in 2013, analyzed the freezing injury resistibility and the remedial mea- sures of ten varieties(combination). [Result] The results showed that for different va- rieties (combinations) of rapeseed, compared cutting ones with non-cutting ones, the plant silique, seeds number and seed weight increased in diverse level, "however, the plant yield remained the same. Correlation analysis suggested that freezing in- jury had the greatest impact on plant silique. [Conclusion] After cutting processing, yield traits were able to partially recover and showed kind of positive correlation with Plant yield.
文摘[Objective]The paper was to explore the influence of near-surface low temperature on cultivation of soft-seed pomegranate,and to provide guidance for planting location of soft-seed pomegranate.[Method]Taking 10 soft-seed pomegranate planting plots under different site conditions as the research objects,the near-surface low temperature of 45-50 cm was dynamically monitored from December 1,2018 to February 20,2019,and comparative analysis was made based on the local meteorological data over the same period.[Result]The near-surface low temperature of each temperature monitoring point was lower than the local meteorological data,which were all in the range of low temperature causing freezing in-jury of pomegranate trees,but the degree of freezing injury was different.The variation of near-surface low temperature was positively correlated with the altitude of terrain,but negatively correlated with the difference of topography and landform.When the local topography and landform were similar,the accumulation time of near-surface low temperature was negatively correlated with the altitude of terrain,while the duration of low tem-perature directly affected the degree of freezing injury.[Conclusion]The development of soft-seed pomegranate cultivation in Tunisia along Huang Mangling region in Henan Province refers to the local meteorological data.Meantime,it is also necessary to pay attention to the impact of regional microclimate environment,especially early monitoring of near-surface temperature to select suitable site and natural conditions.
文摘On the basis of summarizing the research on disaster risk at home and abroad, Shenyang, one of the cities with the most frequent meteorological disasters, was taken as an example. Cold chilling injury is one of the major agricultural meteorological disasters, which poses a serious threat to the sustainable development of agriculture and food security in Jilin and Shenyang. Based on meteorological observation data of recent 65 years in Shenyang and microclimate monitoring data of agricultural facilities in two years, this paper makes statistical analysis by mathematical statistics and calculates the highest outdoor temperature, the lowest indoor air temperature and the highest ceiling temperature according to the meteorological grade index of agricultural facilities’ low temperature chilling damage, low temperature and other meteorological elements of grade indicators, analysis of greenhouse production trend of greenhouse climate change, and to discuss the relationship between temperature inside and outside the greenhouse and the facility microclimate conditions in agricultural cold weather disastrous and early warning services. The results showed that in the 21st century, the number of days with low temperature of ≤-20°C increased significantly compared with those in the 1970s, 1980s and 1990s. The risk of low temperature chilling injury in facility agriculture in Shenyang was dominated by mild and moderate low temperature risk. Shenyang low temperature cold damage warning services standards to reduce the impact of chilling injury on agricultural facilities and development.
基金supported by grants from"Shihuida"Scientific Research Program of Sichuan Province Medical Association of China,No.SHD12-21Medical Scientific Research Program of Health Bureau of Yibin City in China
文摘Non-freezing cold injury is a prevalent cause of peripheral nerve damage, but its pathogenic mechanism is poorly understood, and treatment remains inadequate. Glucocorticoids have anti-inflammatory and lipid peroxidation-inhibiting properties. We therefore examined whether dexamethasone, a synthetic glucocorticoid compound, would alleviate early-stage non-freezing cold injury of the sciatic nerve. We established Wistar rat models of non-freezing cold injury by exposing the left sciatic nerve to cold(3–5°C) for 2 hours, then administered dexamethasone(3 mg/kg intraperitoneally) to half of the models. One day after injury, the concentration of Evans blue tracer in the injured sciatic nerve of rats that received dexamethasone was notably lower than that in the injured sciatic nerve of rats that did not receive dexamethasone; neither Evans blue dye nor capillary stenosis was observed in the endoneurium, but myelinated nerve fibers were markedly degenerated in the injured sciatic nerve of animals that received dexamethasone. After dexamethasone administration, however, endoneurial vasculopathy was markedly improved, although damage to the myelinated nerve fiber was not alleviated. These findings suggest that dexamethasone protects the blood-nerve barrier, but its benefit in non-freezing cold injury is limited to the vascular system.
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund[CX(12)3006]Jiangsu Province Science and Technology Support Program,China(BE2013352)Study on Saving the Cost Facility Cultivation Techniques of High-quality,Safe and Efficient in Fresh Faba Bean(HL2014029)~~
文摘Using Tongxian No.2 as material, the effects of different film-covering time, different sowing time and different planting density on the occurrence of freeze injury and yield of fresh broad beans were investigated. The randomized block design was adopted. The results showed that with the delayed film covering, the incidence of mild freeze injury and number of headless seedlings were increased correspondingly, but the yield was increased; with the delayed sowing, the branch number per plant, effective branch number per plant, incidence of mild freeze injury and number of headless seedlings were all reduced, and the broad beans, sowed on September 30 th, obtained the highest yield; planting density showed on effect on the occurrence of freeze injury, and the yield was increased with the increase of planting density. Under the same film-covering time, the incidence of freeze injury was reduced with the delayed sowing time and it showed no changes when planting density was changed, but the yield was increased with the increase of planting density and it was highest when broad bean seeds were sowed on September 30th;under the same sowing time, the incidence of freeze injury was increased with the delayed film-covering time and it showed no changes when planting density was changed, and the yield was increased with the delayed film-covering time and increased planting density; under the same planting density, the incidence of freeze injury was increased with the delayed film-covering time but was reduced with the delayed sowing time, and the yield was increased with the delayed film-covering time and it was highest when the broad bean seeds were sowed on September30 th. Under same film-covering time and sowing time, the total branch number per plant and effective branch number per plant were reduced, but the yield was increased with the increase of planting density; under same film-covering time and planting density, the incidence of freeze injury was reduced with the delayed sowing time, and the yield was highest when broad bean seeds were sowed on September30th; under same sowing time and planting density, the incidence of freeze injury and the yield were all increased with the delayed film-covering time.
基金supported by grants from the Key Tech-nologies R&D Program of Shanxi Province, China(20060311140)the Open Project Program of Weather Bureau of Shanxi Province, China (SX053001)
文摘Freeze injury is an usual disaster for winter wheat in Shanxi Province, China, and monitoring freeze injury is of important economic significance. The aim of this article is to monitor and analyze the winter wheat freeze injury using remote sensing data, to monitor the occurrence and spatial distribution of winter wheat freeze in time, as well as the severity of the damage. The winter wheat freeze injury was monitored using multi-temporal moderate-resolution imaging spectroradiometer (MODIS) data, combined with ground meteorological data and field survey data, the change of normalized difference vegetation index (NDVI) before and after freeze injury was analyzed, as well as the effect of winter wheat growth recovery rate on yield. The results showed that the NDVI of winter wheat decreased dramatically after the suffering from freeze injury, which was the prominent feature for the winter wheat freeze injury monitoring. The degrees of winter wheat freeze injury were different in the three regions, of which, Yuncheng was the worst severity and the largest freeze injury area, the severity of freeze injury correlates with the breeding stage of the winter wheat. The yield of winter wheat showed positive correlation with its growth recovery rate (r=0.659^** which can be utilized to monitor the severity of winter wheat freeze injury as well as its impact on yield. It can effectively monitor the occurrence and severity of winter wheat freeze injury using horizontal and vertical profile distribution and growth wheat freeze injury in Shanxi Province. recovery rate, and provide a basis for monitoring the winter
基金supported by the National Natural Science Foundation of China (41101395, 41101397 and 41001199)the Beijing New Star Project on Science & Technology,China (2010B024)the Key Technologies R&D Program of China during the 12th Five-Year Plan period(2012BAH29B04)
文摘Winter wheat freeze injury is one of the main agro-meteorological disasters affecting wheat production. In order to evaluate the severity of freeze injury on winter wheat systematically, we proposed a grey-system model (GSM) to monitor the degree and the distribution of the winter wheat freeze injury. The model combines remote sensing (RS) and geographic information system (GIS) technology. It gave examples of wheat freeze injury monitoring applications in Gaocheng and Jinzhou of Hebei Province, China. We carried out a quantitative evaluation method study on the severity of winter wheat freeze injury. First, a grey relational analysis (GRA) was conducted. At the same time, the weights of the stressful factors were determined. Then a wheat freezing injury stress multiple factor spatial matrix was constructed using spatial interpolation technology. Finally, a winter wheat freeze damage evaluation model was established through grey clustering algorithm (GCA), and classifying the study area into three sub-areas, affected by severe, medium or light disasters. The evaluation model were verified by the Kappa model, the overall accuracy reached 78.82% and the Kappa coefficient was 0.6754. Therefore, through integration of GSM with RS images as well as GIS analysis, quantitative evaluation and study of winter wheat freeze disasters can be conducted objectively and accurately, making the evaluation model more scientific.
基金supported by the National Key Research and Development Program of China(2016YFC0500302)the National Nature Scientific Foundation Project of China(31200432)
文摘In forest ecosystems, gap formation changes the allocation of abiotic resources and thus affects the survival and growth of understory plants. However, how tree seedling survival and growth respond to low-temperature events and the influencing mechanisms remain unclear. To clarify how low-temperature event limits the survival and growth of tree seedlings in the montane regions of eastern Liaoning Province, northeast China, we investigated temperature and light intensity within secondary forest gaps, and the survival and growth of Juglans mandshurica seedlings after a low-temperature event in the spring of 2014. Damage to seedlings due to low temperature sig- nificantly varied in different aspects. Seedlings in gaps on southeast-facing slopes were the most seriously damaged, followed by those in gaps on northeast-facing slopes. In contrast, seedlings in west-facing gaps and in control plots without slope aspect were not damaged. The freezing injury index for seedlings was negatively correlated with minimum temperature (r = - 0.608, P 〈 0.01), but it was positively correlated with light intensity (r= 0.818, P 〈 0.01). In addition, height and root collar diameter of damaged seedlings were significantly lower than those of the undamaged seedlings (P 〈 0.01) during the early growing season (April-July), but no significant difference were observed during the late growing season (July-Oc- tober) (P 〉 0.05). The extent of seedling damage was directly related to slope aspect. Low temperature and high light intensity were found to be the dominant factors affecting extent of damage to seedlings on southeast- and northeast-facing slopes.
文摘The susceptibility was compared between murine bone marr ow hemopoietic cells and splenic lymphocytes to four major factors of cryopreservation process: toxicity of dimethyl sulfoxide (DMSO), cooling rate,Tris-NH4Cl treatment, and dilution after thawing. When the concentration of DMSO was over 20%, the proliferative function or viability of both kinds of cells decreased markedly. Injury to hemopoietic stem cells and lymphoeytes was found more severe in rapid cooling than in slow cooling, and the intensity of iujury seemed alike under the same fast cooling condition. Pretreatment of bone marrow cells with Tris-NH4Cl did not significantly accentuate the injury of frozen-thawed hemopoietic stem cells. Dilution of the frozen-thawed cells in ice bath or at a fast rate was harmful to both cells. No significant differential susceptibilities of the two kinds of cells to each factor were found. These results indicate that selective destruction or inactivation of lymphocytes by cold treatment among bone marrow cells may be impossible.
文摘Sperm freezing is widely used in ART clinics around the globe. Very little has actually changed with respect to cryopreservation protocols and methodology of freezing over the last 50 years. The aim of this paper is to briefly review the basic principles that underlie freezing and ice crystal formation and also provide a brief overview of newer sperm freezing techniques like sperm vitrification and freeze drying of sperm.