OBJECTIVE: To observe the clinical efficacy of Bushenjiangya(reinforcing kidney and lowering blood pressure) decoction in curing hypertension of kidney Qi deficiency type. METHODS: First 60 patients with hypertension ...OBJECTIVE: To observe the clinical efficacy of Bushenjiangya(reinforcing kidney and lowering blood pressure) decoction in curing hypertension of kidney Qi deficiency type. METHODS: First 60 patients with hypertension of kidney Qi deficiency type were divided into control group and treatment group randomly; the conventional western medicine was given to patients of the control group for antihypertensive therapy, while the traditional Chinese medicine Bushenjiangya decoction was added for patients in the treatment group based on the therapy for those in control group; after 2 months, the blood pressure, clinical efficacy and urine microalbumin changes of patients in the two groups before and after the treatment were observed. RESULTS: a. The systolic pressure of patients in the two groups was all reduced obviously(P<0.05); b. The total effective rate of experiment group in antihypertensive effect was 76.7%, while that of control group was 83.3%(P>0.05); c. The total effective rate of experiment group in TCM syndrome effect was 86.7%, while that of control group was 63.3%, showing that the effect of experiment group was better than that of control group(P<0.05); d. The 24 h urine microalbumin reduction of experiment group was better than that of control group(P<0.05). CONCLUSIONS: The curative effect of Bushenjiangya decoction in combination with the western medicine is better than that of simple western medicine for hypertension of kidney Qi deficiency type, and also the urine microalbumin can be obviously reduced to protect the kidney function.展开更多
Serine proteinase,purified from the hepatopancreas of Pacific white shrimp(Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen(ASC)isolated from Nile tilapia(Oreochromis sp.)skin to produce angioten...Serine proteinase,purified from the hepatopancreas of Pacific white shrimp(Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen(ASC)isolated from Nile tilapia(Oreochromis sp.)skin to produce angiotensin I-converting enzyme(ACE)inhibitory peptides(ACEIPs).A series of column chromatography assays were used to separate the ACEIPs.A peptide,NPARTCR,was isolated as it exhibited high ACE inhibition potential.Further digestion of this peptide by a proline specific endopeptidase(PSEP),produced a pentapeptide ARTCR with ACE inhibitory activity(IC_(50))of 77.0 pmol/L.Both NPARTCR and ARTCR inhibited ACE in a non-competitive manner.An in vivo study in rats demonstrated that ARTCR has ACE inhibitory activity via lowering systolic blood pressure in spontaneously hypertensive rats(SHRs).These results suggest that processing by-products from shrimp and tilapia are ideal raw materials for the production of serine proteinase and collagen,respectively.Serine proteinase and collagen are both ideal raw materials that can be used to derive ACE inhibitory active peptides against hypertension.展开更多
文摘OBJECTIVE: To observe the clinical efficacy of Bushenjiangya(reinforcing kidney and lowering blood pressure) decoction in curing hypertension of kidney Qi deficiency type. METHODS: First 60 patients with hypertension of kidney Qi deficiency type were divided into control group and treatment group randomly; the conventional western medicine was given to patients of the control group for antihypertensive therapy, while the traditional Chinese medicine Bushenjiangya decoction was added for patients in the treatment group based on the therapy for those in control group; after 2 months, the blood pressure, clinical efficacy and urine microalbumin changes of patients in the two groups before and after the treatment were observed. RESULTS: a. The systolic pressure of patients in the two groups was all reduced obviously(P<0.05); b. The total effective rate of experiment group in antihypertensive effect was 76.7%, while that of control group was 83.3%(P>0.05); c. The total effective rate of experiment group in TCM syndrome effect was 86.7%, while that of control group was 63.3%, showing that the effect of experiment group was better than that of control group(P<0.05); d. The 24 h urine microalbumin reduction of experiment group was better than that of control group(P<0.05). CONCLUSIONS: The curative effect of Bushenjiangya decoction in combination with the western medicine is better than that of simple western medicine for hypertension of kidney Qi deficiency type, and also the urine microalbumin can be obviously reduced to protect the kidney function.
基金This work was sponsored by the National Key R&D Program of China(2018YFD0901004)the National Natural Scientific Foundations of China(31471640,31702372).
文摘Serine proteinase,purified from the hepatopancreas of Pacific white shrimp(Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen(ASC)isolated from Nile tilapia(Oreochromis sp.)skin to produce angiotensin I-converting enzyme(ACE)inhibitory peptides(ACEIPs).A series of column chromatography assays were used to separate the ACEIPs.A peptide,NPARTCR,was isolated as it exhibited high ACE inhibition potential.Further digestion of this peptide by a proline specific endopeptidase(PSEP),produced a pentapeptide ARTCR with ACE inhibitory activity(IC_(50))of 77.0 pmol/L.Both NPARTCR and ARTCR inhibited ACE in a non-competitive manner.An in vivo study in rats demonstrated that ARTCR has ACE inhibitory activity via lowering systolic blood pressure in spontaneously hypertensive rats(SHRs).These results suggest that processing by-products from shrimp and tilapia are ideal raw materials for the production of serine proteinase and collagen,respectively.Serine proteinase and collagen are both ideal raw materials that can be used to derive ACE inhibitory active peptides against hypertension.
