目的通过研究原发性高血压(EH)患者的血清Lp(a)、Lp-PLA2水平与血压变异性(BPV)的特征,进一步探讨EH患者血清中Lp(a)和Lp-PLA2水平与BPV之间的关系。方法选取2022年9月至2023年9月于内蒙古自治区人民医院门诊及住院诊疗的EH患者240例。...目的通过研究原发性高血压(EH)患者的血清Lp(a)、Lp-PLA2水平与血压变异性(BPV)的特征,进一步探讨EH患者血清中Lp(a)和Lp-PLA2水平与BPV之间的关系。方法选取2022年9月至2023年9月于内蒙古自治区人民医院门诊及住院诊疗的EH患者240例。将患者分为A组:Lp(a)、Lp-PLA2均正常,115例;B组:仅Lp(a)升高,48例;C组:仅Lp-PLA2升高,42例;D组:Lp(a)、Lp-PLA2均升高,35例。探索Lp(a)和Lp-PLA2水平与血压变异性之间的相关性。结果B组各时段变异系数(CV)均高于A组(P<0.05);C组24 h SBPCV、24 h DBP CV、nSBP CV、dDBP CV、dSBP CV高于A组(P<0.05);D组各时段CV均高于A组(P<0.05)。Lp(a)、Lp-PLA2与BPV呈正相关(rs>0,P<0.05)。B组、C组以及D组的Lp(a)、Lp-PLA2和24 h SBP CV、24 h DBP CV间均呈显著正相关关系(rs>0,P<0.05)。结论EH患者血清Lp(a)、Lp-PLA2水平与BPV呈正相关,且血清Lp(a)、Lp-PLA2均升高的患者BPV也升高。展开更多
Background The benefits of combining benzoic acid and essential oils(BAO)to mitigate intestinal impairment during the weaning process have been well established,while the detailed underlying mechanism has not been ful...Background The benefits of combining benzoic acid and essential oils(BAO)to mitigate intestinal impairment during the weaning process have been well established,while the detailed underlying mechanism has not been fully elucidated.Previous research has primarily focused on the reparative effects of BAO on intestinal injury,while neglecting its potential in enhancing intestinal stress resistance.Methods In this study,we investigated the pre-protective effect of BAO against LPS-induced stress using a modified experimental procedure.Piglets were pre-supplemented with BAO for 14 d,followed by a challenge with LPS or saline to collect blood and intestinal samples.Results Our findings demonstrated that BAO supplementation led to significant improvements in piglets’final weight,average daily gain,and feed intake/body gain ratio.Additionally,BAO supplementation positively influenced the composition of intestinal microbiota,increasing beneficial Actinobacteriota and Alloprevotella while reducing harmful Desulfobacterota,Prevotella and Oscillospira.Furthermore,BAO supplementation effectively mitigated oxidative disturbances and inflammatory responses induced by acute LPS challenge.This was evidenced by elevated levels of T-AOC,SOD,and GSH,as well as decreased levels of MDA,TNF-α,and IL-6 in the plasma.Moreover,piglets subjected to LPS challenge and pre-supplemented with BAO exhibited significant improvements in intestinal morphological structure and enhanced integrity,as indicated by restored expression levels of Occludin and Claudin-1 compared to the non-supplemented counterparts.Further analysis revealed that BAO supplementation enhanced the jejunal antioxidative capacity by increasing GSH-Px levels and decreasing MDA levels under the LPS challenge and stimulated the activation of the Nrf2 signaling pathway.Additionally,the reduction of TLR4/NF-κB/MAPK signaling pathways activation and proinflammatory factor were also observed in the jejunal of those piglets fed with BAO.Conclusions In summary,our study demonstrates that pre-supplementation of BAO enhances the anti-stress capacity of weaned piglets by improving intestinal microbiota composition,reinforcing the intestinal barrier,and enhancing antioxidative and anti-inflammatory capabilities.These effects are closely associated with the activation of Nrf2 and TLR4/NF-κB/MAPK signaling pathways.展开更多
Objective:To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.Methods:RAW 264.7 cells were treated with 2.5μg/mL of LPS,50...Objective:To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.Methods:RAW 264.7 cells were treated with 2.5μg/mL of LPS,50μM of coumarin,and 50μM eugenol for 24 h.The viability of the cells was assessed using MTT assay.The production of nitric oxide was determined using Griess reagent and DCFH-DA was used to measure the production of reactive oxygen species.The protein expression of NLRP3,IL-1β,NF-κB,and cyclooxygenase 2 was assessed using Western blot analysis.Results:Coumarin and eugenol showed anti-inflammatory effects against LPS-induced inflammatory response by ameliorating the expression of NLRP3 inflammasome and NF-κB,which further led to a subsequent reduction in IL-1β,nitric oxide,and reactive oxygen species.Conclusions:Coumarin and eugenol exert their anti-inflammatory activities by modulating the NLRP3 inflammasome pathway and NF-κB.These compounds may have promising therapeutic applications for the treatment of various inflammatory diseases.展开更多
There is a substantial body of empirical research that has found the fund return distributions to exhibit pronounced peakiness, heavy tails, and skewness, deviating from a normal distribution. Addressing the limitatio...There is a substantial body of empirical research that has found the fund return distributions to exhibit pronounced peakiness, heavy tails, and skewness, deviating from a normal distribution. Addressing the limitations of the traditional Sharpe ratio, which assumes a normal distribution of returns and uses standard deviation to measure investment risk, this paper primarily employs the Value at Risk (VaR) based on Lp quantile to adjust excess returns of funds. This method offers superior robustness, is capable of capturing asymmetry and heavy-tailed characteristics, and is more flexible, providing a better description of the tail risk in fund returns. Empirical studies have shown that using the Sharpe ratio corrected with the Lp quantile is feasible for evaluating and ranking the performance of open-end funds.展开更多
As extremely important methods, Lp regression methods have attracted the attention of either theoretical or empirical researchers all over the world. As special cases of that, quantile and expectile regression (with p...As extremely important methods, Lp regression methods have attracted the attention of either theoretical or empirical researchers all over the world. As special cases of that, quantile and expectile regression (with p = 1 and p = 2, respectively) are well acquainted with. In contrast with them, general Lp regression (with p equals 1 and 2) has recently been found to have many unexpected properties by some studies, especially when 1 p Lp quantile regression under various p settings and shows some recent advances in Lp quantile regression, theoretically and empirically.展开更多
目的以传统中药蒲公英的主要成分蒲公英甾醇为研究对象,探讨蒲公英甾醇的体外抗炎作用及其作用机制。方法培养小鼠腹腔巨噬细胞RAW264.7进行试验分组,检测RAW264.7细胞COX-2和i NOS m RNA和蛋白的表达,并检测ERK 1/2、JNK和p38 MAPK及...目的以传统中药蒲公英的主要成分蒲公英甾醇为研究对象,探讨蒲公英甾醇的体外抗炎作用及其作用机制。方法培养小鼠腹腔巨噬细胞RAW264.7进行试验分组,检测RAW264.7细胞COX-2和i NOS m RNA和蛋白的表达,并检测ERK 1/2、JNK和p38 MAPK及其磷酸化水平的表达。结果蒲公英甾醇分别不同程度的抑制了i NOS和COX-2 m RNA和蛋白的表达,且呈一定的剂量依赖关系;显著抑制了LPS诱导的RAW 264.7细胞p38和ERK 1/2激酶的磷酸化,并呈剂量依赖关系;但对JNK激酶磷酸化的抑制作用不显著。结论蒲公英甾醇通过调节p38和ERK 1/2 MAPKs的磷酸化水平抑制i NOS和COX-2的表达,从而发挥体外抗炎作用。展开更多
文摘目的通过研究原发性高血压(EH)患者的血清Lp(a)、Lp-PLA2水平与血压变异性(BPV)的特征,进一步探讨EH患者血清中Lp(a)和Lp-PLA2水平与BPV之间的关系。方法选取2022年9月至2023年9月于内蒙古自治区人民医院门诊及住院诊疗的EH患者240例。将患者分为A组:Lp(a)、Lp-PLA2均正常,115例;B组:仅Lp(a)升高,48例;C组:仅Lp-PLA2升高,42例;D组:Lp(a)、Lp-PLA2均升高,35例。探索Lp(a)和Lp-PLA2水平与血压变异性之间的相关性。结果B组各时段变异系数(CV)均高于A组(P<0.05);C组24 h SBPCV、24 h DBP CV、nSBP CV、dDBP CV、dSBP CV高于A组(P<0.05);D组各时段CV均高于A组(P<0.05)。Lp(a)、Lp-PLA2与BPV呈正相关(rs>0,P<0.05)。B组、C组以及D组的Lp(a)、Lp-PLA2和24 h SBP CV、24 h DBP CV间均呈显著正相关关系(rs>0,P<0.05)。结论EH患者血清Lp(a)、Lp-PLA2水平与BPV呈正相关,且血清Lp(a)、Lp-PLA2均升高的患者BPV也升高。
基金supported by the National Key Research and Development Program(2021YFD1300400)Natural Science Foundation of Guangdong Province(2021A1515010944)Science and Technology Projects in Guangzhou(202201011730).
文摘Background The benefits of combining benzoic acid and essential oils(BAO)to mitigate intestinal impairment during the weaning process have been well established,while the detailed underlying mechanism has not been fully elucidated.Previous research has primarily focused on the reparative effects of BAO on intestinal injury,while neglecting its potential in enhancing intestinal stress resistance.Methods In this study,we investigated the pre-protective effect of BAO against LPS-induced stress using a modified experimental procedure.Piglets were pre-supplemented with BAO for 14 d,followed by a challenge with LPS or saline to collect blood and intestinal samples.Results Our findings demonstrated that BAO supplementation led to significant improvements in piglets’final weight,average daily gain,and feed intake/body gain ratio.Additionally,BAO supplementation positively influenced the composition of intestinal microbiota,increasing beneficial Actinobacteriota and Alloprevotella while reducing harmful Desulfobacterota,Prevotella and Oscillospira.Furthermore,BAO supplementation effectively mitigated oxidative disturbances and inflammatory responses induced by acute LPS challenge.This was evidenced by elevated levels of T-AOC,SOD,and GSH,as well as decreased levels of MDA,TNF-α,and IL-6 in the plasma.Moreover,piglets subjected to LPS challenge and pre-supplemented with BAO exhibited significant improvements in intestinal morphological structure and enhanced integrity,as indicated by restored expression levels of Occludin and Claudin-1 compared to the non-supplemented counterparts.Further analysis revealed that BAO supplementation enhanced the jejunal antioxidative capacity by increasing GSH-Px levels and decreasing MDA levels under the LPS challenge and stimulated the activation of the Nrf2 signaling pathway.Additionally,the reduction of TLR4/NF-κB/MAPK signaling pathways activation and proinflammatory factor were also observed in the jejunal of those piglets fed with BAO.Conclusions In summary,our study demonstrates that pre-supplementation of BAO enhances the anti-stress capacity of weaned piglets by improving intestinal microbiota composition,reinforcing the intestinal barrier,and enhancing antioxidative and anti-inflammatory capabilities.These effects are closely associated with the activation of Nrf2 and TLR4/NF-κB/MAPK signaling pathways.
基金supported by the Defence Institute of Physiology and Allied Sciences.
文摘Objective:To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.Methods:RAW 264.7 cells were treated with 2.5μg/mL of LPS,50μM of coumarin,and 50μM eugenol for 24 h.The viability of the cells was assessed using MTT assay.The production of nitric oxide was determined using Griess reagent and DCFH-DA was used to measure the production of reactive oxygen species.The protein expression of NLRP3,IL-1β,NF-κB,and cyclooxygenase 2 was assessed using Western blot analysis.Results:Coumarin and eugenol showed anti-inflammatory effects against LPS-induced inflammatory response by ameliorating the expression of NLRP3 inflammasome and NF-κB,which further led to a subsequent reduction in IL-1β,nitric oxide,and reactive oxygen species.Conclusions:Coumarin and eugenol exert their anti-inflammatory activities by modulating the NLRP3 inflammasome pathway and NF-κB.These compounds may have promising therapeutic applications for the treatment of various inflammatory diseases.
文摘There is a substantial body of empirical research that has found the fund return distributions to exhibit pronounced peakiness, heavy tails, and skewness, deviating from a normal distribution. Addressing the limitations of the traditional Sharpe ratio, which assumes a normal distribution of returns and uses standard deviation to measure investment risk, this paper primarily employs the Value at Risk (VaR) based on Lp quantile to adjust excess returns of funds. This method offers superior robustness, is capable of capturing asymmetry and heavy-tailed characteristics, and is more flexible, providing a better description of the tail risk in fund returns. Empirical studies have shown that using the Sharpe ratio corrected with the Lp quantile is feasible for evaluating and ranking the performance of open-end funds.
文摘As extremely important methods, Lp regression methods have attracted the attention of either theoretical or empirical researchers all over the world. As special cases of that, quantile and expectile regression (with p = 1 and p = 2, respectively) are well acquainted with. In contrast with them, general Lp regression (with p equals 1 and 2) has recently been found to have many unexpected properties by some studies, especially when 1 p Lp quantile regression under various p settings and shows some recent advances in Lp quantile regression, theoretically and empirically.
文摘目的以传统中药蒲公英的主要成分蒲公英甾醇为研究对象,探讨蒲公英甾醇的体外抗炎作用及其作用机制。方法培养小鼠腹腔巨噬细胞RAW264.7进行试验分组,检测RAW264.7细胞COX-2和i NOS m RNA和蛋白的表达,并检测ERK 1/2、JNK和p38 MAPK及其磷酸化水平的表达。结果蒲公英甾醇分别不同程度的抑制了i NOS和COX-2 m RNA和蛋白的表达,且呈一定的剂量依赖关系;显著抑制了LPS诱导的RAW 264.7细胞p38和ERK 1/2激酶的磷酸化,并呈剂量依赖关系;但对JNK激酶磷酸化的抑制作用不显著。结论蒲公英甾醇通过调节p38和ERK 1/2 MAPKs的磷酸化水平抑制i NOS和COX-2的表达,从而发挥体外抗炎作用。
