花旗泽仁对胰岛素抵抗大鼠脂肪因子的影响Ⅱ

目的:观察花旗泽仁对2型糖尿病胰岛素抵抗(Insulin Resistance,IR)大鼠血清瘦素(Leptin)和肿瘤坏死因子-a(TNF-a)的影响,探索花旗泽仁改善胰岛素抵抗的作用机制。方法:应用复合脂肪乳灌胃加链脲佐菌素腹腔注射的方法复制2型糖尿病胰岛素抵抗大鼠模型;采用放射性免疫分析法检测肿瘤坏死因子-α(TNF-α)和瘦素(Leptin)水平。结果:花旗泽仁明显降低了血清TNF-α和Leptin水平,差异显著(P<0.01,P<0.05)。结论:花旗泽仁可通过调节血清TNF-α和Leptin水平而改善2型糖尿病胰岛素抵抗状态。

Leptin receptor polymorphism in patients with asthma and its correlation with the disease progression

Objective:To study the leptin receptor Gln223Arg locus polymorphism in patients with asthma and its correlation with the disease progression.Methods: The patients who were diagnosed with bronchial asthma in our hospital between July 2014 and April 2017 were selected as the asthma group for the study, and the healthy volunteers who received physical examination in our hospital during the same period were selected as the control group. The leptin receptor gene Gln223Arg locus polymorphism was determined, and the contents of inflammatory mediators and airway remodeling indexes in serum as well as the contents of immune cells in peripheral blood were determined.Results: Eotaxin, Galectin-3, YKL-40, SAA, IL-33, ADAM33, NE and MMP9 contents in serum as well as Th17, Th9 and Tfh contents in peripheral blood of asthma group of patients with AA+AG genotype and GG genotype were significantly higher than those of control group whereas TIMP1 and TIMP2 contents in serum as well as Treg contents in peripheral blood were significantly lower than those of control group;Eotaxin, Galectin-3, YKL-40, SAA, IL-33, ADAM33, NE and MMP9 contents in serum as well as Th17, Th9 and Tfh contents in peripheral blood of asthma group of patients with AA+AG genotype were significantly higher than those of patients with GG genotype whereas TIMP1 and TIMP2 contents in serum as well as Treg content in peripheral blood were significantly lower than those of patients with GG genotype.Conclusion: The substitution from leptin receptor Gln223Arg locus polymorphism A G replacement in patients with asthma can aggravate the inflammatory response and immune response disorder as well as airway remodeling.

慢性丙型肝炎肝组织学改变与肝脏瘦素表达的关系

目的研究慢性丙型肝炎(CHC)肝组织学改变与肝脏瘦素表达的关系。方法未经治疗的53例成年CHC患者分为肝脏瘦素阳性组26例和阴性组27例,以单纯非酒精性脂肪性肝病(NAFLD)15例作为对照组,比较各组肝脏瘦素阳性率、肝脏炎症分级、纤维化分期和脂肪变分级有无差异。结果 CHC组脂肪变分级程度轻于NAFLD组(χ2=13.20,P<0.05),但两组分别在脂肪变1级和2级的肝脏瘦素阳性率差异无统计学意义(χ2=2.57,0.04,P均>0.05);两组在轻度炎症和轻度纤维化的瘦素表达阳性率差异有统计学意义,CHC组肝脏瘦素阳性率低于NAFLD组(χ2=4.54,6.86,P均<0.05)。两组CHC的肝脏瘦素阳性率在不同肝脏炎症分级、纤维化分期和是否发生肝脂肪变差异无统计学意义(χ2=0.106,0.846,0.000,P均>0.05),CHC的肝脏瘦素阳性率与肝脏炎症分级、纤维化分期、脂肪变分级和血清HCVRNA水平无明显相关性(P均>0.05)。结论 CHC发生肝脂肪变、肝脏炎症和纤维化程度均与肝脏瘦素表达无明显相关。