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黑果枸杞LrANS基因的克隆及表达分析 被引量:3
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作者 王联星 马得森 +5 位作者 史国民 高思丹 郭佳磊 赵伟民 李凤珍 何涛 《中国细胞生物学学报》 CAS CSCD 2019年第10期1929-1937,共9页
以黑果枸杞为材料,利用同源克隆和RACE技术克隆了花青素合成相关基因LrANS(Gen Bank登录号为MK713977)。序列分析表明, LrANS基因cDNA全长为1 527 bp,包含1 290 bp的开放阅读框,编码429个氨基酸;亚细胞定位显示, LrANS主要分布于细胞质... 以黑果枸杞为材料,利用同源克隆和RACE技术克隆了花青素合成相关基因LrANS(Gen Bank登录号为MK713977)。序列分析表明, LrANS基因cDNA全长为1 527 bp,包含1 290 bp的开放阅读框,编码429个氨基酸;亚细胞定位显示, LrANS主要分布于细胞质及细胞膜上;同源序列比对表明, LrANS与烟草Nt ANS的氨基酸序列相似性较高,达到77.2%。RT-PCR分析显示, LrANS基因在根、茎、叶、花、青果、紫果和黑果中均有表达,且在黑果中的表达显著高于其他组织;在紫外胁迫下, LrANS基因的表达显著下降。推测LrANS基因在花青素合成中发挥着重要作用。 展开更多
关键词 黑果枸杞 花青素 lrans基因 紫外胁迫
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过表达LrAN2基因对马铃薯中花青素和糖苷生物碱含量的影响 被引量:2
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作者 李红艳 李洁雅 +3 位作者 李响 叶广继 周云 王舰 《作物学报》 CAS CSCD 北大核心 2023年第4期988-995,共8页
目前对马铃薯(Solanum tuberosum L.)中MYB基因参与的花青素研究较为深入,但对影响马铃薯品质和安全的糖苷生物碱(steroidal glycoalkaloids,SGAs)在不同组织中的变化规律及调控机制尚不完全清楚。LrAN2是黑果枸杞(Lycium ruthenicum)中... 目前对马铃薯(Solanum tuberosum L.)中MYB基因参与的花青素研究较为深入,但对影响马铃薯品质和安全的糖苷生物碱(steroidal glycoalkaloids,SGAs)在不同组织中的变化规律及调控机制尚不完全清楚。LrAN2是黑果枸杞(Lycium ruthenicum)中的MYB基因,与黑果枸杞果实中花青素的积累有关。本研究以野生型大西洋、2种转LrAN2大西洋株系(LrAN2oe#66和LrAN2oe#200)为试验材料,检测不同组织中的花青素和SGAs含量,并对SGAs生物合成相关基因的表达量分析。pH示差法检测花青素发现,仅在转基因植株(LrAN2oe#200)叶片中检测到一定量的花青素(12 mg 100 g-1FW)。高效液相色谱-串联质谱法检测SGAs发现,3个材料不同组织SGAs含量变化为叶片>薯皮>薯肉。薯皮中的SGAs含量无显著性差异;薯肉中LrAN2oe#66的SGAs含量较对照降低、LrAN2oe#200对照显著增加1.3倍,但未超出安全标准(0.2mg g-1FW);叶片中LrAN2oe#66的SGAs含量较对照增加1倍、LrAN2oe#200较对照显著增加3.8倍。qRT-PCR分析基因表达量发现,StHMG1和StSGT2基因受到LrAN2基因的调控在转基因植株中显著上调。本研究结果对马铃薯植株中花青素含量的积累具有指导意义,为进一步解析马铃薯资源中花青素及SGAs的调控机制提供理论依据。 展开更多
关键词 LrAN2基因 马铃薯 花青素 糖苷生物碱
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枸杞MYB转录因子LrAN2在番茄中的过量表达分析 被引量:2
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作者 罗香怡 李云 +3 位作者 曹东 魏乐 宗渊 刘宝龙 《西北农业学报》 CAS CSCD 北大核心 2021年第9期1374-1381,共8页
为明确黑枸杞LrAN2基因在花青素合成代谢中的调控机制,在与枸杞同属的番茄中做了进一步验证。黑枸杞LrAN2基因参与调控黑色果实花青素生物合成代谢,同时诱导烟草产生紫色表型,受限于枸杞转基因组培技术,选择枸杞亲缘物种番茄进行LrAN2... 为明确黑枸杞LrAN2基因在花青素合成代谢中的调控机制,在与枸杞同属的番茄中做了进一步验证。黑枸杞LrAN2基因参与调控黑色果实花青素生物合成代谢,同时诱导烟草产生紫色表型,受限于枸杞转基因组培技术,选择枸杞亲缘物种番茄进行LrAN2转基因功能分析及其调控机制。基于前期分离的LrAN2开放阅读框序列,通过表达载体构建,荧光定量分析,花青素含量的测定以及液相色谱分析方式对转基因番茄进行检测和分析。结果表明,在番茄材料Maker中过量表达LrAN2基因,得到深绿色番茄植株。转基因番茄的各个组织部位均有不同程度的花青素积累,花、叶和根细胞中都存在颜色较深色素沉着,叶片中花青素含量和LrAN2基因的表达量都是最高。LrAN2的过表达上调DFR和F3′5′H的表达水平,同时激活飞燕草色素和矢车菊色素的积累。说明LrAN2基因能够调控花青素的合成。 展开更多
关键词 番茄 花青素 LrAN2
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The effect of single-tree selction system on soil properties in an oriental beech stand of Hyrcanian forest, north of Iran
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作者 Kambiz Abrari Vajari Hamid Jalilvand +2 位作者 Mohammad Reza Pourmajidian Kambiz Espahbodi Alireza Moshki 《Journal of Forestry Research》 SCIE CAS CSCD 2011年第4期591-596,共6页
A case study was conducted in beech forests of northern Iran to determine the effect of the created gaps on some soil properties in beech stand. Changes of soil properties in small (60-150 mE), medium (151-241 mE)... A case study was conducted in beech forests of northern Iran to determine the effect of the created gaps on some soil properties in beech stand. Changes of soil properties in small (60-150 mE), medium (151-241 mE), large (242-332 m^2) and very large (333-550 m^2) gaps, as well as under closed stands were studied eight years after-the gap creation. Soil samples were taken from three depths, 0-10, 10-20 and 20-30 cm. The gaps were different from their around undisturbed stands in terms of the following soil parameters: Mg^+2 concentration of 0-10 cm at medium gap size, bulk density of 10-20 cm at very large gap size as well as K^+ and Ca^+2 concentrations at 20-30 cm at small and large gap sizes, respectively. Furthermore, the size of the gaps had no effect on soil characteristics through the whole profile. Water saturation percent (Sp %) at 0-10cm as well as P and Mg^+2 at 20--30 cm was different amongst undisturbed stands around different gap sizes. The center and the edges of the gap were different only in terms of organic carbon at the depth of 10-20 cm. Significant differences were observed between gaps and closed canopy regarding P and Ca^+2 at depth 0--10 cm and 10-20 cm, respectively. It can be concluded that applied silvicultural system for harvesting trees which created these gaps might be suitable for conservation and forest management in the region. 展开更多
关键词 gap soil Fagus orientalis single-tree selection system northern lran
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Tectonic Setting of the Barm Firuz Lake,Zagros Mountains,Iran:Inferred from Structural and Karstic Evidence 被引量:1
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作者 Babak Samani 《Journal of Earth Science》 SCIE CAS CSCD 2014年第5期932-938,共7页
This paper presents the role of tectonic and karstic processes in the formation and evolution of Barm Firuz Lake within the Zagros Mountains of Iran. This lake with elevation of 3 340 m from sea level is located in th... This paper presents the role of tectonic and karstic processes in the formation and evolution of Barm Firuz Lake within the Zagros Mountains of Iran. This lake with elevation of 3 340 m from sea level is located in the crest of the Barm Firuz anticline. Results show that the structural evolution of the Barme Firuz Lake is related to both tectonic and karst processes. Therefore the term of tectonokarstic has been used for structural evolution of the lake. Structural studies especially on the stylolite structures revealed the occurrence of an important component of simple shear deformation in the study area. Based on structural and karstic evidence around the lake, the fault-dissolution sink model with oblique pure shear component is suggested as kinematic model of the Barm Firuz Lake evolution in the Zagros Mountains of Iran. 展开更多
关键词 TECTONIC KARST Barm Firuz Lake Zagros Mountains lran.
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