A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo I, Hae II...A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo I, Hae III, and RSA I) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P. arcuatum (ME 131), which had the same pattern to P. micans (ME160 and 04). The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electropho- resis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum (ME132) could not be differentiated from P. micans (ME160 and 04), it was probably mis-identified, since they are quite differ- ent morphologically. P. redfieldii (ME138) could also not be distinguished form P. triestinium (ME132), it should be regarded as a synonym of P. triestinium. Unexpectedly, a restriction site was found in P. micans, compared with previous sequence data.展开更多
基金Project supported by National Basic Research Priorities Program (2001CB409701, 2001CB409710) and supported by NSFC (40376040, 40025614)* Project supported by National Basic Research Priorities Program (2001CB409701, 2001CB409710) and supported by NSFC (40376040, 40025614)
文摘A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo I, Hae III, and RSA I) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P. arcuatum (ME 131), which had the same pattern to P. micans (ME160 and 04). The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electropho- resis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum (ME132) could not be differentiated from P. micans (ME160 and 04), it was probably mis-identified, since they are quite differ- ent morphologically. P. redfieldii (ME138) could also not be distinguished form P. triestinium (ME132), it should be regarded as a synonym of P. triestinium. Unexpectedly, a restriction site was found in P. micans, compared with previous sequence data.
