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水稻硅转运子基因的克隆与表达载体的构建 被引量:4
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作者 陈虞超 宋玉霞 +1 位作者 石磊 陈晓军 《西北农业学报》 CAS CSCD 北大核心 2009年第4期191-196,共6页
提取水稻根中总RNA,根据NCBI中水稻硅转运子基因Lsi1(AB222272)、Lsi2(AB222273)的mR-NA序列设计2对引物,采用RT-PCR技术扩增硅转运子基因Lsi1、Lsi2。并利用DNA重组技术分别构建了两个植物表达载体pCAMBIA2300-35S-Lsi1-OCS和pCAMBIA23... 提取水稻根中总RNA,根据NCBI中水稻硅转运子基因Lsi1(AB222272)、Lsi2(AB222273)的mR-NA序列设计2对引物,采用RT-PCR技术扩增硅转运子基因Lsi1、Lsi2。并利用DNA重组技术分别构建了两个植物表达载体pCAMBIA2300-35S-Lsi1-OCS和pCAMBIA2300-35S-Lsi2-OCS。克隆的水稻硅转运子基因Lsi1、Lsi2与NCBI中已发表的核苷酸序列的同源性为99.33%、99.65%,氨基酸同源性都为100%。为后续培育其他硅转基因树木或农作物,增加其硅元素转运能力、提高其抗逆性奠定了实验基础。 展开更多
关键词 硅转运子 Lsi1 lsi2 表达载体
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A New Type Anticorrosion Coating for Ocean Reinforced Concrete Structures 被引量:1
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作者 程琪 耿桂生 +2 位作者 罗锋 吴三余 赵大林 《China Ocean Engineering》 SCIE EI 2000年第3期385-390,共6页
Corrosion of reinforced concrete structures is a serious problem in ocean engineering. As an orientation of study, anticorrosion coating technique is developed and widely applied, but many problems need to be solved. ... Corrosion of reinforced concrete structures is a serious problem in ocean engineering. As an orientation of study, anticorrosion coating technique is developed and widely applied, but many problems need to be solved. LSW-2 type anticorrosion coating for maritime reinforced concrete structures is characterized by sea water resistance, salt fog resistance, moisture and heal resistance as well as impermeability to chlorions. The new type coating can be applied to wet concrete surface by conventional construction technique. It is a breakthrough in solving the above mentioned problem. The paper mainly introduces the test results, the property indices, coating procedure, construction technique and economic benefit of Ihe coating. 展开更多
关键词 LSI V- 2 type anticorrosion coating:property construction techmique reinforced coneret ocean engineering
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