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Cutaneous Myiasis: Is <i>Lucilia cuprina</i>Safe and Acceptable for Maggot Debridement Therapy?
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作者 Howard Joesphia Kingu Simon Kamande Kuria +3 位作者 Martin Herrer Villet Jane Nthekeleng Mkhize Adupa Dhaffala John Michael Iisa 《Journal of Cosmetics, Dermatological Sciences and Applications》 2012年第2期79-82,共4页
Preservation of viable tissue is important in wound management. It is achieved by small, incremental removal of devitalised, necrotic and infected tissues. Maggot debridement therapy (MDT) is used in septic necrotic w... Preservation of viable tissue is important in wound management. It is achieved by small, incremental removal of devitalised, necrotic and infected tissues. Maggot debridement therapy (MDT) is used in septic necrotic wounds that fail to respond to conventional modalities. MDT has relied on Lucilia cuprina, which consumes only necrotic tissues, as opposed to Lucilia cuprina, which devours both flesh and necrotic tissues. Recent findings have shown that L. cuprina consumes mainly necrotic and very small amounts of viable tissues and may be used in MDT where L. sericata is very rare or absent. Here we describe wound healing in a patient from rural South Africa with cutaneous myiasis. Our findings agree with workers who indicated that L. cuprina could be used in MDT. 展开更多
关键词 MAGGOTS Blow Flies lucilia cuprina Mthatha
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Cloning & sequence identification of Hsp27 gene and expression analysis of the protein on thermal stress in Lucilia cuprina 被引量:3
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作者 Manish K. Singh Pramod K. Tiwari 《Insect Science》 SCIE CAS CSCD 2016年第4期555-568,共14页
Hsp27, a highly conserved small molecular weight heat shock protein, is widely known to be developmentally regulated and heat inducible. Its role in thermotolerance is also implicated. This study is a sequel of our ea... Hsp27, a highly conserved small molecular weight heat shock protein, is widely known to be developmentally regulated and heat inducible. Its role in thermotolerance is also implicated. This study is a sequel of our earlier studies to understand the molecular organization of heat shock genes/proteins and their role in development and thermal adaptation in a sheep pest, Lucilia cuprina (blowfly), which exhibits unusually high adaptability to a variety of environmental stresses, including heat and chemicals. In this report our aim was to understand the evolutionary relationship of Lucilia hsp27 gene/protein with those of other species and its role in thermal adaptation. We sequence characterized the Lchsp27 gene (coding region) and analyzed its expression in various larval and adult tissues under normal as well as heat shock conditions. The nucleotide sequence analysis of 678 bps long-coding region of Lchsp27 exhibited closest evolutionary proximity with Drosophila (90.09%), which belongs to the same order, Diptera. Heat shock caused significant en- hancement in the expression ofLchsp27 gene in all the larval and adult tissues examined, however, in a tissue specific manner. Significantly, in Malpighian tubules, while the heatinduced level ofhsp27 transcript (mRNA) appeared increased as compared to control, the protein level remained unaltered and nuclear localized. We infer that Lchsp27 may have significant role in the maintenance of cellular homeostasis, particularly, during summer months, when the fly remains exposed to high heat in its natural habitat. 展开更多
关键词 heat shock protein HSP27 lucilia cuprina thermal stress
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自然条件下铜绿蝇蛹发育形态及其法医学意义 被引量:3
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作者 王贺 王辉 +3 位作者 王卫杰 李嘉 赵文爱 齐莉莉 《昆虫学报》 CAS CSCD 北大核心 2017年第2期222-228,共7页
【目的】研究自然条件下铜绿蝇Lucilia cuprina蛹期蛹壳颜色变化和蛹的形态发育规律从而为死后间隔时间(postmortem interval,PMI)的推断提供理论依据。【方法】在室外自然条件下饲养铜绿蝇直至其化蛹,每12 h取蛹10头,固定、观察,并利... 【目的】研究自然条件下铜绿蝇Lucilia cuprina蛹期蛹壳颜色变化和蛹的形态发育规律从而为死后间隔时间(postmortem interval,PMI)的推断提供理论依据。【方法】在室外自然条件下饲养铜绿蝇直至其化蛹,每12 h取蛹10头,固定、观察,并利用图像分析系统收集并分析蛹壳颜色的变化信息;在体式显微镜下剥离蛹壳,观察蛹体外部形态变化并拍照记录,描述蛹期发育特征。【结果】自然条件下,铜绿蝇蛹壳颜色随时间呈现逐渐加深趋势,尤其在化蛹初期和临近羽化时变化明显,根据采集到的蛹壳颜色RGB值可做出标准色板。蛹的形态发育呈规律性变化,可将蛹期划分为8个阶段,包括隐头期、显头期、棕翅期、黑鬃胸期、黑鬃腹期、半红眼期、红眼期和预成虫期。【结论】在案发现场,蛹壳颜色标准色板可用于目视比色,初步粗略估算蛹期,同时依据蛹期发育阶段进程表可进一步推算化蛹后时间,从而推断PMI。 展开更多
关键词 铜绿蝇 法医昆虫学 嗜尸性昆虫 死后间隔时间 形态发育 标准色板
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不同温度下铜绿蝇蛹期发育形态观察 被引量:3
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作者 王贺 王玲 李泽民 《中国法医学杂志》 CSCD 2008年第5期324-326,I0001,共4页
目的观察不同温度下铜绿蝇蛹形态变化特征规律并进行发育阶段划分。方法在16℃、20℃、24℃、28℃、32℃下饲养铜绿蝇,于化蛹起,每隔12h取样1次,直至半数羽化。解剖样本,在体视显微镜下观察其体表形态变化。结果铜绿蝇蛹形态发育过程包... 目的观察不同温度下铜绿蝇蛹形态变化特征规律并进行发育阶段划分。方法在16℃、20℃、24℃、28℃、32℃下饲养铜绿蝇,于化蛹起,每隔12h取样1次,直至半数羽化。解剖样本,在体视显微镜下观察其体表形态变化。结果铜绿蝇蛹形态发育过程包含Ⅰ蛹体分头、胸、腹;Ⅱ头咽骨贴壁;Ⅲ原头出现;Ⅳ呼吸角破壳;Ⅴ翅与足出现及褐化;Ⅵ鬃毛出现及褐化;Ⅶ复眼轮廓形成及色素化等7个时间标志性特征,并据此将蛹期划分为9个阶段。结论在已知现场环境温度的前提下,蝇蛹形态变化具有阶段性特征,以此划分蛹期阶段,可望作为推断死后间隔时间(PMI)的参考。 展开更多
关键词 法医昆虫学 死后间隔时间 形态变化 铜绿蝇 蛹期
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铜绿蝇幼虫在不同脏器组织源食物上生长发育变化
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作者 王尧 杜琳敏 +2 位作者 袁增琼 李学博 万立华 《重庆医科大学学报》 CAS CSCD 北大核心 2015年第2期285-288,共4页
目的:研究铜绿蝇幼虫在不同组织源的食物上生长发育变化。方法:在26℃恒温、相对湿度70%条件下,用猪的肌肉组织、肝脏组织、脑组织、肌肉与脂肪的混合物(6︰4)分别饲养铜绿蝇幼虫。每隔12 h测体长和体质量,化蛹后测蛹的体长和体质量。... 目的:研究铜绿蝇幼虫在不同组织源的食物上生长发育变化。方法:在26℃恒温、相对湿度70%条件下,用猪的肌肉组织、肝脏组织、脑组织、肌肉与脂肪的混合物(6︰4)分别饲养铜绿蝇幼虫。每隔12 h测体长和体质量,化蛹后测蛹的体长和体质量。统计幼虫及蛹的死亡率。推算各组幼虫发育总历期和成虫的性别比。结果:40%脂肪混合组与其他3组相比生长缓慢,达到最大个体的时间延迟24 h以上。肝脏组幼虫体长(15.02±1.39)mm和40%脂肪混合组体长(14.85±1.43)mm明显小于脑组织组(17.53±2.08)mm、肌肉组(16.82±2.15)mm(F=5.440,P=0.003);肝脏组幼虫体质量(72.8±14.2)mg和40%脂肪混合组体质量(70.4±15.4)mg均明显小于脑组织组(89.6±19.2)mg和肌肉组(85.9±16.7)mg(F=3.320,P=0.030)。40%脂肪混合组蛹体长及体重均明显小于脑组织组和肌肉组(F=7.890,P=0.001;F=9.280,P=0.001)。40%脂肪混合组幼虫和蛹的死亡率均明显高于脑组织组、肌肉组、肝脏组(F=3.470,P=0.040;F=4.720,P=0.035)。结论:铜绿蝇幼虫在40%脂肪混合食物上生长发育历期较长,肝脏组和40%脂肪混合组幼虫及蛹的体长和体质量较小。 展开更多
关键词 死后间隔时间 铜绿蝇幼虫 法医昆虫学
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铜绿蝇蜕皮激素受体在杆状病毒载体系统中的表达
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作者 代小江 Huang Q Sue 庞义 《病毒学报》 CAS CSCD 北大核心 2000年第2期189-192,共4页
The baculovirus expression vector, Trichoplusia ni nucleopolyhedrovirus, with the advantage of polyhedral inclusion body formation in recombinant viruses, was used to express the ecdysteroid receptor of the Australian... The baculovirus expression vector, Trichoplusia ni nucleopolyhedrovirus, with the advantage of polyhedral inclusion body formation in recombinant viruses, was used to express the ecdysteroid receptor of the Australian sheep blowfly Lucilia cuprina(LcEcR). pSXIVVI +X3/2 baculovirus transfer vector was chosen for a 2.8kb LcEcR cDNA subcloning since pSXIVVI +X3/2 contains an efficient translational initiation signal (ATG) and it allows the LcEcR cDNA fusion to N-terminal codons in the correct reading frame. The resulting transfer plasmid pSXIVVI +X3-LcEcR was cotransfected into BT1-Tn-5B1-4 cells with the parental virus TnNPV-SVI -G minus polyhedral inclusion body, which expresses β-galactosidase gene. After 3~4 runs of plaque purification, three TnNPV-LcEcR clones were obtained with the LcEcR gene under the dual control of synthetic and XIV promoters. These three TnNPV-LcEcR clones all showed white phenotype when stained with X-gal. Western blot analysis showed 2~3 specific polypeptides with molecular weight ranging from 70~90kD. Three TnNPV-LcEcR clones expressed different level of LcEcR polypeptides in BT1-Tn-5B1-4 cells. The TnNPV-LcEcR-1 clone expressed the highest level of LcEcR polypeptides in BT1-Tn-5B1-4 cells 48~72h post infection. 展开更多
关键词 铜绿蝇 蜕皮激素受体 杆状病毒载体系统
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